Use of LoopDeelab during the COVID-19 Pandemic: An Innovative Device for Field DiagnosisArticle Published on 2022-09-172022-11-15 Journal: Viruses [Category] SARS, 치료기술, [키워드] analysed Analysis attack rate collected Concordance conducted COVID COVID-19 COVID-19 outbreak Device diagnosis of SARS-CoV-2 diagnostic double-blind Epidemics evaluate facilitate field France Health Infection intrinsic knowledge LAMP PCR Loopdeelab Loopdeetect management Measures molecular nasopharyngeal swab specimen PCR Rapid RAPID COVID Research protocol RT-LAMP RT-PCR SARS-CoV-2 Seegene, Korea Sensitivity and specificity [DOI] 10.3390/v14092062 PMC 바로가기
Development and Analytical Validation of a One-Step Five-Plex RT-ddPCR Assay for the Quantification of SARS-CoV-2 Transcripts in Clinical SamplesArticle Published on 2022-09-132022-11-16 Journal: Analytical Chemistry [Category] SARS, 치료기술, [키워드] analytical sensitivity Analytical specificity analyzed applied calibrator Characteristics clinical Concordance controls correlation COVID-19 pandemic development Direct DNA and RNA in-silico Inclusion Internal control linear methodology Metrics molecular primer probe provide Quality control quantification Region reproducibility RNA RT-qPCR Sample SARS-CoV-2 SARS-CoV-2 detection sensitivity Spearman specimen Standardization transcripts validation viral concentrations [DOI] 10.1021/acs.analchem.2c00868 PMC 바로가기
Differences in systemic and mucosal SARS-CoV-2 antibody prevalence in a prospective cohort of Dutch childrenArticle Published on 2022-09-092022-11-15 Journal: Frontiers in Immunology [Category] SARS, 진단, [키워드] 95% CI affected antibodies antibody antibody assay Antibody assays Antibody prevalence association children clinical variables cohort study Concordance cross-sectional Demographic variables difference evaluate healthy children Humoral immunity IgA IgG IgG positivity Immunity Immunocompromised infected children Infection investigated medical service MONITOR mucosal mucosal antibody response mucosal IgG Netherland Non-invasive performed positive Prevalence prospective cohort RBD RBD-specific IgG Receptor binding domain reduced Saliva saliva antibodies. SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 spike serum Sex Symptom vaccination [DOI] 10.3389/fimmu.2022.976382 PMC 바로가기
Clinical sensitivity of rapid antigen test during a COVID-19 outbreak in Taipei, May to June 2021Research article Published on 2022-09-012022-10-05 Journal: Journal of the Formosan Medical Association = Taiw [Category] SARS, 진단, 치료기술, [키워드] Accuracy acute respiratory syndrome Antigen antigen detection city clinical Community Concordance coronavirus COVID-19 COVID-19 outbreak Cycle threshold value diagnosing diagnosis of SARS-CoV-2 diagnostic early phase evaluated identify implementation individual individuals infected individual Infection linear moderate outbreak Patient PCT positive Prevalence prevalence of COVID-19 quarantine Rapid Rapid antigen detection tests Result Reverse transcription-polymerase chain reaction RT-PCR RT-PCR assay SARS-CoV-2 SARS-COV-2 infection sensitivity study population Taipei tested [DOI] 10.1016/j.jfma.2022.03.016 [Article Type] Research article
Validation of laboratory developed serology assays for detection of IgG antibody to severe acute respiratory syndrome coronavirus 2 in the South African population남아프리카 인구에서 중증 급성 호흡기 증후군 코로나바이러스 2에 대한 IgG 항체 검출을 위해 실험실에서 개발한 혈청학 분석의 검증Article Published on 2022-09-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 African antibody approved assays authority Beta characterization circulating collected commercial immunoassay Concordance confirmation continent Control coronavirus country Coverage cross-reactive antibodies cut-off value Delta demonstrated described determine diagnostic differentiation ELISA enzyme IFA IgA IgG IgG antibody IgM immunosorbent Infection infection rate investigated Laboratory Laboratory developed lateral flow limit Local mammalian molecular pandemic Patient persistence Population populations positive positive predictive values Predictive predictive values product Quality assurance resource respiratory RT-PCR RT-PCR positive patient SARS-CoV-2 SARS-CoV-2 spike protein sensitivity Serological assay serological assays serology assay serology assays Serology assays. serum sample serum samples severe acute respiratory syndrome Coronavirus shown small sample size South South Africa specificity Spike protein Surveillance tested Vaccine validation variant [DOI] 10.1016/j.jviromet.2022.114571 PMC 바로가기 [Article Type] Article
Patients with infectious diseases undergoing mechanical ventilation in the intensive care unit have better prognosis after receiving metagenomic next-generation sequencing assayResearch article Published on 2022-09-012022-10-05 Journal: International Journal of Infectious Diseases [Category] 비임상, [키워드] 28-day mortality Analysis analyzed approaches assays assigned baseline Concordance Culture diagnostic evaluate Health hypertension ICU Immunosuppressed independent risk factor Infectious disease intensive care intensive care unit mechanical ventilation medical record metagenomic Metagenomic next-generation sequencing Mortality Next-generation sequencing objective on mechanical ventilation pathogen Pathogens diagnosis Patient polymerase chain Prognosis prognosis of patient receiving reducing Result severe pneumonia single-center the patient traditional methods [DOI] 10.1016/j.ijid.2022.07.062 [Article Type] Research article
Rapid and qualitative identification of SARS-CoV-2 mutations associated with variants of concern using a multiplex RT-PCR assay coupled with melting analysisArticle Published on 2022-09-012022-11-16 Journal: International Journal of Infectious Diseases [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] amplified Analysis Beta characterized Complete Concordance contributing to Delta detect Direct E484K examined expected faster Gamma Genetic variant L452R melting Melting analysis multiplex Mutation mutations N501Y Nasopharyngeal swab nasopharyngeal swabs omicron positive public health Rapid Rapid identification RT-PCR RT-PCR assay Sanger method Sanger sequencing SARS-CoV-2 SARS-CoV-2 mutation SARS-CoV-2 variants sensitivity Sensitivity and specificity Seven specificity Spread Surveillance the SARS-CoV-2 variant variants of concern Viral load was performed [DOI] 10.1016/j.ijid.2022.06.032 PMC 바로가기
Development of a simple genotyping method based on indel mutations to rapidly screen SARS-CoV-2 circulating variants: Delta, Omicron BA.1 and BA.2SARS-CoV-2 순환 변이체: Delta, Omicron BA.1 및 BA.2를 신속하게 스크리닝하기 위한 indel 돌연변이에 기반한 간단한 유전형 분석법 개발Article Published on 2022-09-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] Analytical specificity circulating circulating SARS-CoV-2 variants concern Concordance COVID-19 case Delta Delta VOC develop development facilitate flexible genotyping identify implementation melting curve analysis Mutation mutations omicron Partial partial sequencing performed primer public health qPCR assay qPCR assays S gene SARS-CoV-2 Screening screening method Sequencing verification VOCs [DOI] 10.1016/j.jviromet.2022.114570 PMC 바로가기 [Article Type] Article
Comparison between nucleic acid amplification tests, antigen immunofluorescence assay, and in vitro infectivity in SARS-CoV-2 diagnosisArticle Published on 2022-09-012022-11-16 Journal: Brazilian journal of microbiology : [publication o [Category] COVID19(2023년), SARS, 치료기술, [키워드] amplification analyzed Antigen antigen test Cell Concordance correlated cycle threshold Diagnosis functional greater immunofluorescence assay in vitro increased risk infect Infection initial Laboratory NAAT Nasopharyngeal swab nucleic acid amplification nucleic acid amplification test POCT point-of-care positive Rapid test response SARS-CoV-2 SARS-CoV-2 detection specimen Swab tested viral load quantification virus transmission [DOI] 10.1007/s42770-022-00758-6 PMC 바로가기
Limited Correlation between SARS-CoV-2 Serologic Assays for Identification of High-Titer COVID-19 Convalescent Plasma Using FDA ThresholdsArticle Published on 2022-08-312022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] antibody assay standardization assays authorization can be used CCP clinical trials Clinical use Cohort collected comparable Concordance consequence convalescent convalescent plasma COVID-19 COVID-19 convalescent plasma criteria Cutoff cutoff value Donor drug Emergency Emergency use authorization EUA Expanded Access Program FDA FDA EUA FIVE food high-titer CCP high-titer convalescent plasma highlight hospitalized patients identification identify immunosuppressed patients indicate Limited lowest monoclonal antibody multiple serological assay Pandemics Pathogens positive precluded provided ranged reduce released Roche SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant serologic serologic assay serological Serological assay serological assays serology serum sample significantly Standardization therapeutic therapeutic option therapy threshold Treatment [DOI] 10.1128/spectrum.01154-22 PMC 바로가기