Insight into molecular characteristics of SARS-CoV-2 spike protein following D614G point mutation, a molecular dynamics studyD614G 점 돌연변이에 따른 SARS-CoV-2 스파이크 단백질의 분자 특성에 대한 통찰력, 분자 역학 연구Article Published on 2022-08-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 변종, [키워드] addition Amino acid amino acid sequence asymmetric bioinformatics catastrophic changes in Characteristics D614G D614G mutation Effect effective high mortality rate insight molecular molecular dynamics mutant Mutation negative charge Point mutation polar positive Positive Charge Protein public health Receptor binding residue risk factor SARS-CoV-2 SARS-CoV-2 spike protein secondary structure sequence spike stability Substitution tertiary structure the SARS-CoV-2 Treatment virus [DOI] 10.1080/07391102.2021.1872418 PMC 바로가기 [Article Type] Article
Evolutionary dynamics of SARS-CoV-2 circulating in Yogyakarta and Central Java, Indonesia: sequence analysis covering furin cleavage site (FCS) region of the spike protein족자카르타와 인도네시아 중부 자바에서 순환하는 SARS-CoV-2의 진화 역학: 스파이크 단백질의 푸린 절단 부위(FCS) 영역을 포괄하는 서열 분석Article Published on 2022-08-012022-09-11 Journal: International microbiology : the official journal [Category] COVID19(2023년), SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Analysis cDNA cDNA synthesis circulating clade Cluster Clusters confirmed COVID-19 patients coronavirus COVID-19 pandemic COVID-19 patient D614 variant D614G D614G mutation evolutionary dynamics FCS FIVE furin furin cleavage furin cleavage site G614 variant Genome GISAID indicated Indonesia Mutation Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples new SARS-CoV-2 new virus PCR amplification Protein Q677H Region replaced responsible retrieved RNA SARS-CoV-2 SARS-CoV-2 isolate SARS-CoV-2 isolates sequence Sequence analysis Sequencing severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 silent spike Spike protein synthesis the spike protein were used [DOI] 10.1007/s10123-022-00239-8 PMC 바로가기 [Article Type] Article
Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike설계된 이황화물은 잠긴 SARS-CoV-2 스파이크의 구조적 역학을 보여줍니다Article Published on 2022-07-292022-09-11 Journal: PLoS Pathogens [Category] COVID19(2023년), SARS, 변종, [키워드] Acidic Alter Analysis cellular compartment change changes in conformation conformational change conformations D614G D614G mutation Disulfide disulfide bond domain extracellular space functional high-resolution structure hinge interact modulate Mutation Neutral pre-fusion Protein provide RBD Receptor binding domain Regulation reveal SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 spike SARS-CoV-2 spikes sequence Structure structures transition variants of concern viral fitness virion virus wild-type SARS-CoV-2 [DOI] 10.1371/journal.ppat.1010583 PMC 바로가기 [Article Type] Article
Heterologous saRNA Prime, DNA Dual-Antigen Boost SARS-CoV-2 Vaccination Elicits Robust Cellular Immunogenicity and Cross-Variant Neutralizing Antibodies이종 saRNA 프라임, DNA 이중 항원 부스트 SARS-CoV-2 백신 접종으로 강력한 세포 면역원성과 교차 변형 중화 항체 유도Article Published on 2022-07-152022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료법, [키워드] Adenovirus animal model animal models B.1.617.2 Beta boost CD4+ CD8+ T-cell combinations COVID-19 D614G mutation Delta distribution DNA dual dual antigen ENCODE enhanced expression followed by furin furin cleavage region Glutamine Heterologous highest homologous homologous or heterologous Humoral response humoral responses IgG immune response immune responses immunogenicity lipid carrier MHC class I MHC class I and II mice motif N antigen Neutralizing nucleic acid nucleocapsid omicron peptide peptides prefusion conformation prime proline prolines protease pseudovirus receiving regimen regimens Region response RNA vaccine SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant self-amplifying RNA sequence sera serotype spike Stimulation strain Strains Support T-cell T-cell responses vaccination Vaccine Vaccines variant wild type Wuhan [DOI] 10.3389/fimmu.2022.910136 PMC 바로가기 [Article Type] Article
Split T7 promoter-based isothermal transcription amplification for one-step fluorescence detection of SARS-CoV-2 and emerging variantsSARS-CoV-2 및 신흥 변이체의 1단계 형광 검출을 위한 분할 T7 프로모터 기반 등온 전사 증폭Article Published on 2022-07-152022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] 16S rRNA addition amplification applicability applied Bacterial coronavirus disease D614G mutation detect evidence of experimental evidence fluorescence detection isothermal isothermal amplification less Light-up RNA aptamer multiplex nasopharyngeal pandemic promoter RNA rRNA SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants selective sensitivity Sensitivity and specificity specificity split Split T7 promoter STAR T7 RNA polymerase Three-way junction Transcription variant Viral RNA viral RNA detection [DOI] 10.1016/j.bios.2022.114221 PMC 바로가기 [Article Type] Article
Clinical and virologic factors associated with outcomes of COVID-19 before and after vaccination among Veterans: Retrospective analysis from six New England states제대군인의 백신 접종 전후 코로나19 결과와 관련된 임상 및 바이러스학적 요인: 뉴잉글랜드 6개 주의 후향적 분석Article Published on 2022-07-012022-09-11 Journal: Journal of hospital medicine [Category] COVID19(2023년), SARS, 변종, [키워드] 30-Day mortality Admission affecting age Analysis B.1 B.1.617.2 clinical clinical and demographic factors Clinical outcome clinical outcomes Cohort correlated COVID-19 D614G mutation demographic factor died England Epidemiology Factor Healthcare system Hospitalization ICU care increasing age infected with COVID-19 intensive care intensive care unit admission Lineage male MOST New Older outcome required sequenced the mean vaccination veteran veterans viral genomes were infected white with COVID-19 women [DOI] 10.1002/jhm.12852 PMC 바로가기 [Article Type] Article
Structural Dynamics of the SARS-CoV-2 Spike Protein: A 2-Year Retrospective Analysis of SARS-CoV-2 Variants (from Alpha to Omicron) Reveals an Early Divergence between Conserved and Variable EpitopesSARS-CoV-2 스파이크 단백질의 구조 역학: SARS-CoV-2 변이체(알파에서 오미크론까지)의 2년 후향적 분석을 통해 보존된 에피토프와 가변 에피토프 간의 초기 차이가 드러났습니다.Article Published on 2022-06-152022-09-11 Journal: Molecules [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] abrogated ACE2 binding ADE analyzed antibodies antibody Antibody-dependent enhancement antiviral strategy approaches bind binding circulating conformational change conserved COVID-19 infection D614G D614G mutation Delta determined by Divergence domain Dynamics epitope Epitopes Evolution facilitate facilitating antibodies flat help host cells linear mimicking molecular Molecular epidemiology Mutation N-terminal domain Neutralizing Neutralizing antibodies neutralizing antibody NTD omicron pandemic Retrospective analysis risk SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 genomes SARS-CoV-2 strains SARS-CoV-2 variants selected spike Spike protein the SARS-CoV-2 the spike protein Vaccine Variability variable was determined [DOI] 10.3390/molecules27123851 PMC 바로가기 [Article Type] Article
The phylodynamics of SARS-CoV-2 during 2020 in Finland Research Published on 2022-06-102022-10-05 Journal: Communications Medicine [Category] 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome analyses Analysis B.1 lineage Bayesian caused circulating collected coronavirus country D614G mutation early stage Effect European fatality Finnish Google mobility data incidence independent Infection Intervention investigated Italy Lineage pandemic prevalent public health measures replaced Result SARS-CoV-2 SARS-CoV-2 lineage sequence sequenced Spain Spread strain subsequent suggested virus virus genome [DOI] 10.1038/s43856-022-00130-7 [Article Type] Research
Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction현장 진료 실시간 중합효소 연쇄반응을 이용한 SARS-CoV-2 동시 검출 및 스파이크 D614G 돌연변이 식별Article Published on 2022-06-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Analysis applied Chain Reaction contagiousness coronavirus COVID-19 D614G D614G mutation detect Detection sensitivity detection system Diagnosis equipment high mortality Human labeling Laboratory Molecular diagnosis Multiple multiplex Mutation PCR platform POCT point-of-care polymerase chain polymerase chain reaction probe probes rapid detection method Real-time PCR respiratory virus respiratory viruses reverse transcription PCR rRT-PCR SARS-CoV-2 SARS-CoV-2 mutant SARS-CoV-2 mutants Sensitivity and specificity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Simultaneous specificity spike Spike D614G mutation. strain was reduced wild type [DOI] 10.1016/j.jviromet.2022.114513 PMC 바로가기 [Article Type] Article
A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assayHRM 및 프로브 기반 RT-PCR 분석을 사용하여 쿠르드-이란 인구의 다섯 번째 피크에서 SARS-COV-2의 우세한 변이를 식별하기 위한 스크리닝 전략Article Published on 2022-06-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, [키워드] caused country D614G D614G mutation Del69-70 mutation diagnostic test dominant variant E484K E484K mutation E484Q mutation genomic Genomic surveillance high resolution HRM identify indicated Iran Kurdistan L452R L452R mutation melting mortality rate Mutation operation overlapped positive patient probe Probe-based RT-PCR reduce Resolution RT-PCR RT-PCR assay SARS CoV SARS CoV-2 SARS-CoV-2 positive patients screened screening strategy Sequencing suggested Surveillance T478K T478K mutation. Transmissibility variant variants whole-genome sequencing [DOI] 10.1016/j.jviromet.2022.114514 PMC 바로가기 [Article Type] Article