Deregulation of ceRNA Networks in Frontal Cortex and Choroid Plexus of Brain during SARS-CoV-2 Infection Aggravates Neurological Manifestations: An Insight from Bulk and Single-Cell Transcriptomic AnalysesSARS-CoV-2 감염 중 뇌의 전두엽 피질 및 맥락총 신경총에서 ceRNA 네트워크의 규제 완화는 신경 증상을 악화시킵니다: 대량 및 단일 세포 전사체 분석의 통찰력Article Published on 2022-08-012022-09-11 Journal: Advanced Biology [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] Analysis analyzed Brain cell type cell types choroid Choroid plexus competing competing endogenous RNA network cortex deregulated differentially expressed downregulated downregulation downstream excitatory explain Frontal Gene Expression help Human brain impairment indicated Initially insight landscape lncRNA MALAT1 mapping mechanism MIRLET7BHG miRNA miRNAs mRNA NEAT1 network neurological Neuron neurons NorAD pathway enrichment Plexus reconstructed regulatory circuit RNA RNA-Seq SARS-CoV-2 single-cell RNA-seq STXBP5 STXBP5-AS1 therapeutic Therapeutics transcriptomic upregulated upregulation Variability [DOI] 10.1002/adbi.202101310 PMC 바로가기 [Article Type] Article
GMI, a protein from Ganoderma microsporum, induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virusGanoderma microsporum의 단백질인 GMI는 SARS-CoV-2 Spike-pseudotyped 바이러스의 감염을 완화하기 위해 ACE2 분해를 유도합니다Article Published on 2022-08-012022-09-11 Journal: Phytomedicine : international journal of phytother [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] ACE2 ACE2 expression activating addition alleviate bafilomycin blocking Cell cell viability cells cloned Cytometry deaths Degradation determined by downregulated dynamin Effect Efficacy examined exhibit expressing flow cytometry fluorescence microscopy fluorescent function Fungal immunomodulatory proteins fungal protein GFP GMI green fluorescent protein HEK293T host cells Human immunomodulatory immunomodulatory functions in vitro induce infecting Infection inhibited inhibiting Interaction lentiviral lung epithelial lung tissue lysosome macrophages mechanism MG132 mice MTT nebulizer not affect overexpressing pandemic Prevent prevention proteasome Protein protein level pseudovirus reduced rescued respiratory responsible S2 domain SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus infection Severe acute respiratory syndrome spike Spike protein Therapeutic strategies triggered Type Vaccine viability viral infection virus was determined western blot [DOI] 10.1016/j.phymed.2022.154215 PMC 바로가기 [Article Type] Article
Human surfactant protein D facilitates SARS-CoV-2 pseudotype binding and entry in DC-SIGN expressing cells, and downregulates spike protein induced inflammation인간 계면활성제 단백질 D는 DC-SIGN 발현 세포에서 SARS-CoV-2 유사형 결합 및 진입을 촉진하고 스파이크 단백질 유도 염증을 하향 조절합니다Article Published on 2022-07-282022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, [키워드] ACE2 Analysis Anti-viral binding carbohydrate cause Cell cell interaction cell line cell surface receptor cells chemokine chemokines collectins controls COVID-19 cytokine cytokine response Cytokines DC-SIGN dendritic cell domain downregulate downregulated eight epithelial expressing facilitate fragment of HEK 293T HEK293T cell HEK293T cells help Human IFN-α IL- 6 IL-1β IL-8 immune function immune response immunomodulatory effect increase independent independent of infection assay infection assays Inflammation inhibit SARS-CoV-2 innate immune innate immune system Interaction intercellular involved Macrophage macrophages MHC class MHC class II modulating mRNA expression mRNA level mRNA levels N-terminal naïve T cell naïve T cells NF-κB pathogen Pathogens pro-inflammatory cytokine pro-inflammatory cytokines Protein pseudotype RANTES receptors Replication rfhSP-D. RNA virus RT-qPCR SARS- CoV-2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2 spike protein shown SIGN spike Spike protein surface receptor T cells the Spike THP-1 TNF-α treated Treatment Vero Cell Vero cells viral entry viruses [DOI] 10.3389/fimmu.2022.960733 PMC 바로가기 [Article Type] Article
SARS-CoV-2 nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl – accumulation in respiratory epitheliumSARS-CoV-2 뉴클레오캡시드 단백질은 단백질-단백질 상호작용 매개 세포내 Cl-호흡 상피에서의 축적을 통해 과염증을 유발합니다Article Published on 2022-07-272022-09-11 Journal: Signal Transduction and Targeted Therapy [Category] COVID19(2023년), SARS, 신약개발, 치료법, [키워드] abrogated Airway inflammation CAMP cell lines cells CFTR Concentration COVID-19 cystic fibrosis Cytokine storms Defense demonstrated depleting downregulated elicit epithelial epithelial cell epithelial cells expression fibrosis glucocorticoid Host host defense human airway epithelial hyperinflammation immune response immune responses Inflammatory response Inflammatory responses inhibition inhibitor Intracellular knockout microRNA modulating murine model murine models N protein pathogen phosphodiesterase Phosphorylation promoted Protein pulmonary inflammation raised reducing regulated respiratory epithelium resulting robust Rolipram SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleocapsid second messenger selective serum SGK1 signaling pathway Smad3 structural protein suggested sustained targeting therapeutic strategy transmembrane Trigger upregulation [DOI] 10.1038/s41392-022-01048-1 PMC 바로가기 [Article Type] Article
Human Lung Fibroblasts Exhibit Induced Inflammation Memory via Increased IL6 Gene Expression and Release인간 폐 섬유아세포는 증가된 IL6 유전자 발현 및 방출을 통해 유도된 염증 기억을 나타냅니다Article Published on 2022-07-222022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] ACTA2 affected Analysis ANOVA Cell cell line cell lines chronic respiratory disease chronic respiratory diseases Col1a1 contribute COVID-19 determine determined by downregulated ELISA fibroblast fibroblasts fibrosis followed by Gene Expression Human human lung IL1B IL2 IL6 IL8 Immunity Increased induced Inflammatory inflammatory memory Inflammatory response inhibition inhibitor interleukin-6 investigated involved lung memory Mmp8 mRNA expression multiple groups NF-κB not affect pathway Pathways Poly (I:C) POSTN predict qRT-PCR Release respiratory diseases respond SARS-CoV-2 SARS-CoV-2 spike protein significantly significantly increased Simultaneous Simultaneous treatment Spike protein statistical significance subsequent TGFB1 TLR3 TNFα trained immunity treated Treatment two-way ANOVA viral infection was determined were measured were used Withdrawal [DOI] 10.3389/fimmu.2022.921728 PMC 바로가기 [Article Type] Article
Transcriptional landscape of human neuroblastoma cells in response to SARS-CoV-2SARS-CoV-2에 대한 인간 신경모세포종 세포의 전사적 풍경Article Published on 2022-07-062022-09-11 Journal: BMC neuroscience [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] Activation acute respiratory syndrome acute respiratory syndrome coronavirus Analysis applied approach Cell cells Central nervous system changes in chemokines contagious coronavirus Cytokines decrease differentially expressed gene Differentially expressed genes downregulated function functions Gene ontology host mRNA identify Infection Injury involved knowledge Kyoto mechanism mRNA mRNAs Neuroinflammation Neurological symptom Neurological symptoms NF-κB pathogenic Pathways Patient performed receptors reported RNA RNA sequencing SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 signaling pathways significantly SK-N-SH cells. transcriptional profile transcriptional profiles transcriptomic Treatment upregulated [DOI] 10.1186/s12868-022-00728-6 PMC 바로가기 [Article Type] Article
Sars-Cov-2 Spike Protein-Induced Damage of hiPSC-Derived CardiomyocytesSars-Cov-2 스파이크 단백질에 의한 hiPSC 유래 심근세포 손상Article Published on 2022-07-012022-09-12 Journal: Advanced Biology [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] ACE2 alteration Ang II angiotensin-converting enzyme 2 Cardiomyocytes chromatin cleavage complex connexin Culture damage downregulated Effect endocytic expression fluctuation functional hiPSC host cells incubated investigated margination molecular pluripotent stem cell Protein Proteins receptor SARS-CoV-2 spike Spike protein Spike proteins Structure subunit the spike protein undergo virus while [DOI] 10.1002/adbi.202101327 PMC 바로가기 [Article Type] Article
Sequence-Specific Features of Short Double-Strand, Blunt-End RNAs Have RIG-I- and Type 1 Interferon-Dependent or -Independent Anti-Viral Effects짧은 이중 가닥, 평활 말단 RNA의 서열 특이적 특징은 RIG-I 및 유형 1 인터페론 의존성 또는 독립적 항바이러스 효과를 갖는다Article Published on 2022-06-282022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, 치료제, [키워드] adaptors Adenovirus Anti-viral anti-viral effect Antiviral Antiviral agents Antiviral effect antiviral effects assays Cell cell cycle cell cycle genes cell line cells cellular complementary cytoplasmic DNA DNA virus downregulated dsRNA dsRNAs Effect elicited fibroblast Gene Expression H3N2 human airway IFN IFN-alpha IFN-I IFNAR2 independent independent of indicated Infection Influenza influenza A influenza A virus Influenza virus interferon knock-out library MDA5 mechanism mimicking molecular patterns MYD88 organism Organisms pathogen pathogen-associated molecular patterns Pathogens Randomized receptor required retinoic acid retinoic acid-inducible gene I RIG-I RNA RNA therapy RNA virus RNAs SARS-CoV-2 sequence short double-strand blunt-end RNA. transcriptomics transfection Type ZNFX1 [DOI] 10.3390/v14071407 PMC 바로가기 [Article Type] Article
Monocytic-Myeloid Derived Suppressor Cells Suppress T-Cell Responses in Recovered SARS CoV2-Infected Individuals단핵구 골수 유래 억제 세포는 회복된 SARS CoV2에 감염된 개인의 T 세포 반응을 억제합니다Article Published on 2022-06-242022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제, [키워드] adaptive immune responses Antigen arginase caused CD11b CD14 CD33 CD57 Cell characterized clinical recovery coronavirus disease Coronavirus disease 2019 correlated COVID-19 CX3CR1 CXCL1 CXCL2 cytokine production Cytokine storm Cytokines depleting dominant downregulated DQA1 DQB1 effector memory expansion Frequency function functional functions Gene Expression HLA-B HLA-DQA1 HLA-DQB1 HLA-DR Hyperinflammatory Hyperinflammatory response IL-6 IL1β immune immune activation immune responses individual individuals infected individual Infection membrane monocytic-myeloid derived suppressor cells mononuclear cell NLRP3 nucleocapsid pathway Pathway analysis Pathways PBMC PD1 peptide peptide pool peptide pools Peripheral blood Peripheral blood mononuclear cells plasma quantity recall recovered regulate remained response RNA-Seq ROS SARS Coronavirus SARS coronavirus 2 SARS CoV2 infection shown spike glycoprotein subset suppress suppresse suppressed suppressor T cell T cell response T cell responses T-cell T-cell responses target TGFβ transcriptional transcriptomic transcriptomic (RNA-Seq). Vaccine viral infection virus [DOI] 10.3389/fimmu.2022.894543 PMC 바로가기 [Article Type] Article
Integration of omics data to generate and analyse COVID-19 specific genome-scale metabolic modelsomics 데이터를 통합하여 COVID-19 특정 게놈 규모 대사 모델 생성 및 분석Article Published on 2022-06-012022-09-11 Journal: Computers in biology and medicine [Category] 바이오마커, 유전자 메커니즘, 치료제, [키워드] addressed analyses Analysis Antiviral applied approach cholesterol cholesterol metabolism code Combination complex Context-specific models COVID-19 COVID-19 patients dataset datasets disease down-regulated downregulated downregulation enrichment FIVE generate genome-scale metabolic models host response include indicated Infection integration MEM metabolic Metabolic enrichment analysis metabolic pathway metabolic pathways metabolism Model Model extraction methods. observation observations predicted prediction preserved Previous studies previous study produced provided retinol separated targets transcriptomic Variability Vitamin D3 [DOI] 10.1016/j.compbiomed.2022.105428 PMC 바로가기 [Article Type] Article