Attenuation of SARS-CoV-2 replication and associated inflammation by concomitant targeting of viral and host cap 2′-O-ribose methyltransferasesArticle Published on 2022-09-012022-11-15 Journal: The EMBO Journal [Category] COVID19(2023년), SARS, 치료제, [키워드] AHCY Anti-SARS-CoV-2 Activity Antiviral antiviral activity antiviral drug antivirals attenuation Concomitant COVID-19 COVID-19 treatments Drug screening DZNep enzyme Ex vivo exhibit functional Host host-directed hyperinflammation identify Immune escape immunomodulatory effect in silico in vitro in vivo independent Infection Inflammation inhibitor Interaction Lung fibrosis marker Methylation methyltransferase pathogen reduce SAM SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication Side effects Stage suppresse Treatment Viral RNA virus replication [DOI] 10.15252/embj.2022111608 PMC 바로가기
Replication of SARS-CoV-2 Omicron BA.2 variant in ex vivo cultures of the human upper and lower respiratory tractArticle Published on 2022-09-012022-11-16 Journal: EBioMedicine [Category] SARS, 변종, [키워드] airway antibody bronchial Bronchial tissue characterized China clinical manifestation Committee comparable Culture Delta delta variant disease severity Ex vivo explain explained Hong Kong human lung tissue immune evasion Infectious disease Lower respiratory tract lung lung tissue nasal nasal tissue observation omicron Omicron BA.2 parenchymal pathogenicity Patient Region replaced replicated Replication respiratory tissue SARS-CoV-2 strain Strains supported tested tissue Transmissibility Transmission university variant virus viruses wild-type wild-type virus [DOI] 10.1016/j.ebiom.2022.104232 PMC 바로가기
Anti-PEG Antibodies Boosted in Humans by SARS-CoV-2 Lipid Nanoparticle mRNA VaccineArticle Published on 2022-08-232022-11-16 Journal: ACS Nano [Category] COVID19(2023년), SARS, 치료기술, [키워드] antibodies antibody association biomolecular coronas Blood BNT162b2 cell interaction cell interactions clinical impact clinically correlated COVID-19 dose elevated environmental exposure Ex vivo glycol Human human blood assay identify IgG IgM immunoglobulins increase in Lipid LNP Moderna mRNA mRNA vaccination mRNA vaccine mRNA-1273 nanoparticle Neutralizing antibody response overcome particle−immune cell interactions. PEGylated lipid nanoparticle Pfizer-BioNTech phagocyte plasma receiving SARS-CoV-2 SARS-CoV-2 vaccine significant increase significantly systemic reactogenicity the SARS-CoV-2 vaccination Vaccine [DOI] 10.1021/acsnano.2c04543 PMC 바로가기
A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike인간화된 나노바디 파지 디스플레이 라이브러리는 SARS CoV-2 스파이크의 강력한 결합제를 생성합니다Article Published on 2022-08-102022-09-11 Journal: PLoS ONE [Category] SARS, 변종, 진단, [키워드] (alpha affinity antibody antibody treatment B.1.1.7 binding modes complex effective Ex vivo humanized mitigate molecular dynamics Molecular dynamics simulation molecular dynamics simulations N501Y N501Y mutation nanobodies nanobody neutralization Neutralizing Neutralizing antibodies primary human airway RBD RBD mutation reducing SARS CoV-2 SARS-CoV-2 SARS-CoV-2 infections SARS-CoV-2 spike protein SARS-CoV-2 variants shown Spike protein Structural analysis the SARS-CoV-2 variant Viral viral burden WA1 [DOI] 10.1371/journal.pone.0272364 PMC 바로가기 [Article Type] Article
Nonproductive exposure of PBMCs to SARS-CoV-2 induces cell-intrinsic innate immune responsesSARS-CoV-2에 대한 PBMC의 비생산적 노출은 세포 고유의 타고난 면역 반응을 유도합니다Article Published on 2022-08-012022-09-11 Journal: Molecular Systems Biology [Category] SARS, 치료기술, [키워드] absence ACE2 receptor analyzed baseline bystander cell bystander cells Cell COVID-19 Culture Cytokines detectable Ex vivo experiment exposure to expression expression profile expression profiles healthy donor healthy donors human PBMC immune responses in vivo induce infected cell Infection innate immune response Interaction interferon interferon-stimulated gene interferon-stimulated genes interferon-stimulated genes. Invasion ISG ISGs Mild monocyte Monocytes mounted Particle Patient PBMC PBMCs physical proinflammatory cytokines respiratory tract response RT-PCR SARS-CoV SARS-CoV-2 severe COVID-19 Single-cell RNA sequencing STAT stimulate susceptibility triggered virus particle virus particles [DOI] 10.15252/msb.202210961 PMC 바로가기 [Article Type] Article
Exosomal Vaccine Loading T Cell Epitope Peptides of SARS-CoV-2 Induces Robust CD8 + T Cell Response in HLA-A Transgenic MiceSARS-CoV-2의 엑소좀 백신 로딩 T 세포 에피토프 펩티드는 HLA-A 형질전환 마우스에서 강력한 CD8 + T 세포 반응을 유도합니다Article Published on 2022-07-292022-09-11 Journal: International journal of nanomedicine [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 adjuvant Administered CD8 control group Coronavirus-2 current elicited epitope epitope peptide Ex vivo exosomal vaccine Exosome Exosomes fold followed by fraction frequencies Frequency fused generate HLA-A human leukocyte antigen human leukocyte antigen-A IFN-γ induce injected intracellular cytokine staining leukocyte mice Modification Neutralizing antibodies neutralizing antibody NMR nuclear nuclear magnetic resonance organ toxicity peak peptide peptides purification purified response robust SARS-CoV-2 SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus significantly subcutaneously T cell T cell response T cell responses T cells the SARS-CoV-2 virus the severe acute respiratory syndrome coronavirus-2. transgenic mice transmission electron microscopy Vaccine vaccine group western blotting wild-type [DOI] 10.2147/IJN.S367494 PMC 바로가기 [Article Type] Article
Transient and durable T cell reactivity after COVID-19COVID-19 이후 일시적이고 지속적인 T 세포 반응성Article Published on 2022-07-262022-09-11 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), MERS, SARS, 진단, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus analyzed Antigen antigens Cell convalescent patient Convalescent patients coronavirus correlated COVID-19 cytokine Cytokines determine Ex vivo followed by IL-12 IL-13 IL-17 IL-2 Infection interferon interferon-γ interleukin long-lasting Longevity median N-terminal nucleocapsid protein Patient peptide peptides Polarity Protein reactivity remained respiratory retrieved SARS-CoV-2 severe acute respiratory syndrome Coronavirus stimulated synchronous T cell T cell cytokines T cell memory T helper T helper cells Transient triggered whole blood sample [DOI] 10.1073/pnas.2203659119 PMC 바로가기 [Article Type] Article
Longitudinal Assessment of SARS-CoV-2-Specific T Cell Cytokine-Producing Responses for 1 Year Reveals Persistence of Multicytokine Proliferative Responses, with Greater Immunity Associated with Disease Severity1년 동안 SARS-CoV-2-특이적 T 세포 사이토카인 생성 반응에 대한 종단 평가는 질병 중증도와 관련된 더 큰 면역과 함께 다중 사이토카인 증식 반응의 지속성을 보여줍니다Article Published on 2022-07-132022-09-11 Journal: Journal of Virology [Category] COVID19(2023년), SARS, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 all subjects assessment average CD4 CD8 Cell Cell-mediated immunity clone coronavirus Coronaviruses Critical cytokine cytokine response Cytokines declined demonstrated dominant ELISPOT ELISpot assay envelope enzyme-linked immunosorbent epitope Epitopes Ex vivo female Frequency gamma interferon granzyme B greater GzmB highlight IFN-γ IL-2 immune immune modeling. Immunity immunodominant immunodominant epitope immunodominant epitopes induce Infection interferon interleukin interleukin 2 kill longitudinal male median membrane nucleocapsid observation other coronaviruses peaked persistence post-symptom onset potent predicted produced producing proliferative PSO reduced regions response robust SARS-CoV-1 SARS-CoV-2 seasonal coronavirus seasonal coronaviruses severe acute respiratory syndrome Coronavirus severe disease structural protein structural proteins subject survivor T cell T cell immunity T cell memory T cell response T cell responses T cells target cells Vaccine viral infections Year [DOI] 10.1128/jvi.00509-22 PMC 바로가기 [Article Type] Article
SARS-CoV-2-specific T cell memory with common TCRαβ motifs is established in unvaccinated children who seroconvert after infection일반적인 TCRαβ 모티프를 가진 SARS-CoV-2-특이 T 세포 기억은 감염 후 혈청 전환되는 예방접종을 받지 않은 어린이에서 확립됩니다Article Published on 2022-07-122022-09-11 Journal: Immunity [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Adults analyzed B cells CD4 CD4(+) T cells CD8 CD8(+) T cells Cell children circulating clonal expansion comparable convalescent coronavirus COVID-19 Epitopes Ex vivo HLA class I Infection memory memory T memory T cell memory T cells motif ORF1a phenotype presenting recruited reduced respiratory response SARS-CoV-2 SARS-CoV-2 epitopes seroconvert seroconverted Seven severe acute respiratory syndrome Coronavirus stem cell T cell T cell memory T cell receptor T cells TCR sequencing tetramer tetramer-specific. to define vaccination virus [DOI] 10.1016/j.immuni.2022.06.003 PMC 바로가기 [Article Type] Article
Beneficial ex vivo immunomodulatory and clinical effects of clarithromycin in COVID-19Research article Published on 2022-07-012022-10-05 Journal: Journal of infection and chemotherapy : official j [Category] COVID19(2023년), SARS, 임상, [키워드] addition amoxicillin Anti-bacterial agents Antibiotics benefit CD4+ CD8+ clarithromycin Clinical effect Co-incubation confounder COVID COVID-19 COVID-19 patients Critical care Critically ill patient Cytokines decrease Effect Ex vivo had no healthy hospital ICU patient IFN-alpha IL-10 IL-2 IL-6 immunomodulation immunomodulatory immunomodulatory properties immunomodulatory property in viral increase in infections intensive care intracellular cytokine investigated lymphocyte Macrolide monocyte no effect PBMC phagocytosis reduction in mortality Result retrospective cohort study SARS-CoV-2 Spike protein stimulated synergistic T cell response TNF-alpha Univariate analysis was performed with COVID-19 [DOI] 10.1016/j.jiac.2022.04.001 [Article Type] Research article