Single-Point Mutations in the N Gene of SARS-CoV-2 Adversely Impact Detection by a Commercial Dual Target Diagnostic AssaySARS-CoV-2의 N 유전자에 있는 단일점 돌연변이는 상업용 이중 표적 진단 분석에 의한 역효과 검출Research Article Published on 2021-11-172022-09-10 Journal: Microbiology Spectrum [Category] MERS, SARS, 변종, 진단, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 acute SARS-CoV-2 infection amplicons amplification amplified assays binding Cepheid Cepheid Xpert coronavirus Critical detection determine Diagnosis diagnostic diagnostic assay diagnostic tests early diagnosis eight FIVE gene target Genetic genomic gold gold standard highlight Impact knowledge Mutation N gene nucleic acid amplification tests occur pandemic patients PCR product point mutations position Rapid diagnostic test Real-time PCR Region reported respiratory responsible SARS-CoV-2 SARS-CoV-2 genome sequenced Sequencing severe acute respiratory syndrome Coronavirus single-point mutation single-point mutations target the SARS-CoV-2 genome whole genome whole genomes [DOI] 10.1128/Spectrum.01494-21 PMC 바로가기 [Article Type] Research Article
Concordance between PCR-based extraction-free saliva and nasopharyngeal swabs for SARS-CoV-2 testingResearch Article Published on 2021-10-152022-10-31 Journal: HRB Open Research [Category] COVID-19, [키워드] 95% CI Accuracy acute respiratory syndrome analysed Analysis Asymptomatic Cohort Concordance coronavirus correlation COVID-19 testing demonstrated detect extraction-free method gene target gold standard greater hospital invasive less matching Nasopharyngeal swab nasopharyngeal swabs NPs nucleic acid participant Population positive processed protocol RdRP recruited Result RNA RT-qPCR Saliva saliva specimens saliva test SalivaDirect SARS-CoV-2 SARS-CoV-2 testing SARS-CoV2 sensitivity significant difference specificity specimens symptomatic Symptomatic patients [DOI] 10.12688/hrbopenres.13353.2 PMC 바로가기 [Article Type] Research Article
A SARS-CoV-2 mutant from B.1.258 lineage with ∆H69/∆V70 deletion in the Spike protein circulating in Central Europe in the fall 20202020 년 가을 중부 유럽에서 순환하는 스파이크 단백질에서 ∆H69/∆V70 삭제 된 B.1.258 계보로부터의 SARS-COV-2 돌연변이 체Short Report Published on 2021-08-272022-09-10 Journal: Virus Genes [Category] MERS, SARS, 변종, [키워드] ∆H69/∆V70 Analysis analyzed B.1.1.7 B.1.1.7 variant B.1.177 B.1.258 caused circulating collected Czech Republic Deletion domain Europe European country fraction gene target import in some Lineage mutant N-terminal domain N439K substitution NTD Phylogenetic analysis Protein RBD Receptor-binding domain Region replaced REPLACED BY RT-qPCR test SARS-CoV-2 SARS-CoV-2 mutant SARS-CoV-2 mutants Sequencing Slovakia spike Spike protein supplementary material the receptor-binding domain the Spike variant virus [DOI] 10.1007/s11262-021-01866-5 PMC 바로가기 [Article Type] Short Report
Discrepancy between PCR based SARS-CoV-2 tests suggests the need to re-evaluate diagnostic assaysResearch Note Published on 2021-08-172022-10-27 Journal: BMC Research Notes [Category] COVID-19, [키워드] Accuracy amplification conducted COVID-19 COVID19 diagnosis of COVID-19 diagnostic discrepancy eight examined gene target Initially investigated maintain Mild Modification new SARS-CoV-2 objective pathogen Patient PCR PCR assay PCR assays PCR efficiency positive primer binding sites reaction reduced Regular respiratory respiratory sample Result Roche SARS-CoV-2 SARS-CoV-2 test severe COVID-19 spike spike gene strain Strains supplementary material TaqPath tested the patient variant Variation [DOI] 10.1186/s13104-021-05722-5 PMC 바로가기 [Article Type] Research Note
Discrepancy between PCR based SARS-CoV-2 tests suggests the need to re-evaluate diagnostic assaysResearch Note Published on 2021-08-172022-10-27 Journal: BMC Research Notes [Category] Coronavirus, COVID-19, MERS, SARS, [키워드] Accuracy amplification conducted COVID-19 COVID19 diagnosis of COVID-19 diagnostic discrepancy eight examined gene target Initially investigated maintain Mild Modification new SARS-CoV-2 objective pathogen Patient PCR PCR assay PCR assays PCR efficiency positive primer binding sites reaction reduced Regular respiratory respiratory sample Result Roche SARS-CoV-2 SARS-CoV-2 test severe COVID-19 spike spike gene strain Strains supplementary material TaqPath tested the patient variant Variation [DOI] 10.1186/s13104-021-05722-5 PMC 바로가기 [Article Type] Research Note
Discrepancy between PCR based SARS-CoV-2 tests suggests the need to re-evaluate diagnostic assaysPCR 기반 SARS-COV-2 테스트 간의 불일치는 진단 분석을 다시 평가해야합니다.Research Note Published on 2021-08-172022-09-10 Journal: BMC Research Notes [Category] 변종, 치료기술, 치료제, [키워드] Accuracy amplification conducted COVID-19 COVID19 diagnosis of COVID-19 diagnostic discrepancy eight examined gene target Initially investigated maintain Mild Modification new SARS-CoV-2 objective pathogen Patient PCR PCR assay PCR assays PCR efficiency positive primer binding sites reaction reduced Regular respiratory respiratory sample Respiratory samples Result robustness Roche SARS-CoV-2 SARS-CoV-2 test severe COVID-19 spike spike gene strain Strains supplementary material TaqPath tested the patient variant Variation [DOI] 10.1186/s13104-021-05722-5 PMC 바로가기 [Article Type] Research Note
Prolonged detection of complete viral genomes demonstrated by SARS-CoV-2 sequencing of serial respiratory specimens연속 호흡기 시편의 SARS-COV-2 시퀀싱에 의해 입증 된 완전한 바이러스 게놈의 장기 검출Research Article Published on 2021-08-052022-09-10 Journal: PLoS ONE [Category] MERS, SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 advanced disease can not clinically Complete confirmed case confirmed cases coronavirus COVID19 patient COVID19 patients demonstrated Diagnosis disease stage early stage gene target Infection initial mapped methodology MONITOR N gene nCoV NGS positive Prevent Prolonged RdRP Real-time PCR reduced required respiratory respiratory sample respiratory specimen SARS-CoV-2 SARS-CoV-2 sequencing sequence Sequencing severe acute respiratory syndrome Coronavirus Spread Stage stages tested the disease the SARS-CoV-2 Transcription triplex Viral viral genome viral sequence viral sequences Viral transmission whole genome [DOI] 10.1371/journal.pone.0255691 PMC 바로가기 [Article Type] Research Article
The role of microRNAs in modulating SARS-CoV-2 infection in human cells: a systematic review인간 세포에서 SARS-CoV-2 감염을 조절하는 microRNA의 역할: 체계적인 검토Article Published on 2021-07-012022-09-11 Journal: Infection, genetics and evolution : journal of mol [Category] SARS, 변종, [키워드] ACE2 addition Analysis applied article association caused cell interactions cleavage Complex network Complex networks COVID-19 COVID-19 patients Critical database disease eligibility criteria Evidence expression level expression levels function Gene Expression gene target Genes Genome Genome sequencing highlight host cells host genes host miRNA host miRNAs host responses human disease human infection in silico independent infected cell Infection infections Interaction microRNA MicroRNAs miRNA miRNAs modulating MOST Mutation Pathologies predicted Prospective PROSPERO Proteins protocol provide Registered regulate reported Research review SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 isolate SARS-CoV-2 isolates searched selected Sequencing specimen suggested systematic review therapeutic target therapeutic targets TMPRSS2 translation viral genome virus virus infection [DOI] 10.1016/j.meegid.2021.104832 PMC 바로가기 [Article Type] Article
A Multiplex and Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Sensitive and Rapid Detection of Novel SARS-CoV-2새로운 SARS-CoV-2의 민감하고 신속한 검출을 위한 다중 및 비색 역전사 루프 매개 등온 증폭 분석Cellular and Infection Microbiology Published on 2021-06-292022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] 95% CI Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Care caused Chain Reaction clinical clinical samples Coefficient of variation Cohen’s kappa collected comparable coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 cross-reaction demonstrated detection develop diagnosis of SARS-CoV-2 diagnostic Diagnostic accuracy diagnostic sensitivity early diagnosis faster feature gene target Health Health care help individual Infection isothermal amplification LAMP Large-scale testing limit of detection Loop-mediated isothermal amplification membrane molecular multiplex Multiplex RT-LAMP novel ORF1ab gene Outbreaks pandemic Pathogens point-of-care polymerase chain reaction Precision Prevent Primary Health Care primer primer sets public health Rapid rapid molecular tests RdRP reaction reagent real-time reverse transcriptase-polymerase chain reaction resource-limited settings respiratory respiratory pathogen Respiratory pathogens reverse transcriptase reverse transcriptase-polymerase chain reaction reverse transcription RNA sample RNA-dependent RNA polymerase room temperature RT-LAMP RT-qPCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections sensitive severe acute respiratory syndrome Coronavirus specificity Swab swabs temperature tested timely diagnosis Variation Viral Viral RNA viruses [DOI] 10.3389/fcimb.2021.653616 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2Multiplex qPCR은 SARS-CoV-2의 글로벌 감시를 강화하기 위해 우려 변종을 식별합니다.Methods and Resources Published on 2021-05-072022-08-13 Journal: PLoS Biology [Category] Medicine and Health Sciences, SARS, 변종, 치료기술, 치료제, [키워드] 501Y.V1 501Y.V2 acute respiratory syndrome coronavirus B.1.1.7 B.1.351 Brazil can not circulating coronavirus coronavirus 2 COVID-19 Deletion detect ENhance escape gene target immune responses Laboratory lack lineages Local multiplex ORF1a other variant other variants P.1 PCR assay qPCR respiratory SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants Sequencing severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 South Africa Spread Surveillance TaqPath Thermo Fisher these viruses Transmissibility United Kingdom variant variants variants of concern viruses VoC [DOI] 10.1371/journal.pbio.3001236 PMC 바로가기 [Article Type] Methods and Resources