Remdesivir, Molnupiravir and Nirmatrelvir remain active against SARS-CoV-2 Omicron and other variants of concernArticle Published on 2022-02-012022-10-27 Journal: Antiviral Research [Category] COVID-19, [키워드] active against Alpha Antiviral antiviral activity antivirals Beta Cell Compound conserved Delta delta variant EIDD-1931 FIVE Gamma GFP GS-441524 high-content imaging in vitro Infection molnupiravir MPro Nirmatrelvir observation omicron other variant parent nucleoside Post-infection protease Protease inhibitor Remdesivir RNA polymerase SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV2 serial dilution susceptible target gene target protein variants of concern Viral RNA virus VoC was determined [DOI] 10.1016/j.antiviral.2022.105252 PMC 바로가기 [Article Type] Article
Remdesivir, Molnupiravir and Nirmatrelvir remain active against SARS-CoV-2 Omicron and other variants of concernArticle Published on 2022-02-012022-10-27 Journal: Antiviral Research [Category] Coronavirus, COVID-19, COVID19(2023년), Fulltext, SARS, [키워드] active against Alpha Antiviral antiviral activity antivirals Beta Cell Compound conserved Delta delta variant EIDD-1931 FIVE Gamma GFP GS-441524 high-content imaging in vitro Infection molnupiravir MPro Nirmatrelvir observation omicron other variant parent nucleoside Post-infection protease Protease inhibitor Remdesivir RNA polymerase SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV2 serial dilution susceptible target gene target protein variants of concern Viral RNA virus VoC was determined [DOI] 10.1016/j.antiviral.2022.105252 PMC 바로가기 [Article Type] Article
Remdesivir, Molnupiravir and Nirmatrelvir remain active against SARS-CoV-2 Omicron and other variants of concernRemdesivir, Molnupiravir 및 Nirmatrelvir는 SARS-Cov-2 Omicron 및 기타 변형에 대해 계속 활성화되어 있습니다.Article Published on 2022-02-012022-09-10 Journal: Antiviral Research [Category] 변종, 치료제, [키워드] active against Alpha Antiviral antiviral activity antivirals Beta Cell Compound conserved Delta delta variant Delta variants determined by EIDD-1931 FIVE Gamma GFP GS-441524 high-content imaging Highlights in vitro Infection molnupiravir MPro Nirmatrelvir nucleoside observation omicron other variant parent nucleoside Post-infection protease Protease inhibitor Remdesivir RNA polymerase SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV2 serial dilution serial dilutions susceptible target gene Target genes target protein target proteins variants variants of concern Viral Viral RNA virus VoC was determined [DOI] 10.1016/j.antiviral.2022.105252 PMC 바로가기 [Article Type] Article
Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and ReplicationArticle Published on 2022-01-182022-10-28 Journal: Viruses [Category] COVID-19, SARS, [키워드] A97V antiviral activity autonomous BAC Bacterial Cell culture chromosome clone coronavirus COVID-19 COVID-19 pandemic decrease drug Efficacy exhibited experiments F480L facilitated fluorescent full-length SARS-CoV-2 functional Genes genomic region genomic RNA GFP growth Healthcare system in vitro Infectious virus inhibited membrane modify molecular clone Mutation nanoluciferase non-infectious Nsp12 P323L phenotype platform polymerase polymorphism Protein provide recombinant Remdesivir Replication replicon reporter Research resistance RNA virus SARS-CoV-2 SARS-CoV-2 mutants SARS-CoV-2 susceptibility SARS-CoV-2 variants sequence Substitution susceptibility System transcript Treatment utility Vaccines Vero E6 cell viral gene virus virus isolate [DOI] 10.3390/v14020172 PMC 바로가기 [Article Type] Article
Development of a Single-Cycle Infectious SARS-CoV-2 Virus Replicon Particle System for Use in Biosafety Level 2 LaboratoriesVirus-Cell Interactions Published on 2021-12-012022-10-30 Journal: Journal of Virology [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] 3CL activity acute respiratory syndrome addition anti-CoV drug antiviral drug antiviral drugs biosafety Biosafety Level 2 Biosafety Level 3 BSL2 BSL3 Cell cell types conditions contagious coronavirus CoV decrease deleting development drug dose ENCODE expressing expression fluorescent GFP glycoprotein glycoprotein G host cells Immunity Infection Laboratory lack murine mutant neutralized Particle platform Protease inhibitor Protein Remdesivir replication of SARS-CoV-2 replicon reporter reporters Research robust S gene SARS-CoV-2 SARS-CoV-2 biology SARS-CoV-2 research SARS-CoV-2 virus Scientific community serum spike gene Support System Treatment Vaccine variety vesicular stomatitis virus viral envelope viral genome viral glycoproteins VSV [DOI] 10.1128/jvi.01837-21 PMC 바로가기 [Article Type] Virus-Cell Interactions
Novel suction-based in vivo cutaneous DNA transfection platformResearch Article Published on 2021-11-052022-10-28 Journal: Science Advances [Category] COVID-19, MERS, SARS, [키워드] absence antibody production applied Chinese correlation demonstrated description DNA DNA uptake DNA vaccine Efficiency Electroporation expression expression patterns GFP histological Host humoral immune response in vivo indicate injection middle modeling moderate notable novel Plasmid platform provide SARS-CoV-2 Specific Strong Therapies tissue injury utility Vaccines [DOI] 10.1126/sciadv.abj0611 PMC 바로가기 [Article Type] Research Article
Susceptibility of Dog, Hamster, and Mouse Cells to the Replication-Competent Adenovirus 11p E1/E3 Green Fluorescence Protein Vector Has Implications for the Selection of Animal Vaccine ModelsMicrobiology Published on 2021-10-272022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] Ad5 Adenovirus animal animal cells animal model Antigen can be used candidate CD46 Cell cell line cell lines cellular receptor CHO cells Critical cytotoxicity DNA replication dog dose E1/E3 vector Efficacy Express expressed expression Fluorescence generate GFP GFP expression green hamster host cell Human in vitro experiments infect infecting Infection manifested MDCK cells Model mouse not express Protein qPCR assay RCAd11pGFP SARS-CoV-2 vaccine Selection specificity susceptibility the vaccine Trial undetectable Vaccine Vaccines vector viral DNA virus virus infection virus particle [DOI] 10.3389/fmicb.2021.698999 PMC 바로가기 [Article Type] Microbiology
A simple, sensitive, and low‐cost FACS assay for detecting antibodies against the native SARS‐CoV‐2 spike protein천연 SARS-CoV-2 스파이크 단백질에 대한 항체를 검출하기 위한 간단하고 민감하며 저렴한 FACS 분석Original Article Published on 2021-05-122022-09-10 Journal: Immunity, Inflammation and Disease [Category] SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antibodies antibody Antibody Response Antibody responses Antibody titer Antibody titers applied Asymptomatic Care CHO cell CHO cells coronavirus COVID‐19 COVID‐19 patient COVID‐19 patients detectable develop Disease progression donors Epidemic expression vector expression vectors FACS flow cytometry Germany GFP Hamburg Health Health care HEK cell help hospital hospitalized patients household contacts human serum identify individual Infectious diseases Infectious virus lab Microscopy MONITOR nuclear Protein Recombinant protein recombinant proteins remained respiratory responses SARS‐CoV‐2 secondary antibody social distancing spike Spike protein Symptom symptomatic contacts the epidemic transfected cell transfected cells trials utility vaccination Virology virus [DOI] 10.1002/iid3.446 PMC 바로가기 [Article Type] Original Article
Sensitivity of infectious SARS-CoV-2 B.1.1.7 and B.1.351 variants to neutralizing antibodies Research Published on 2021-03-262022-10-05 Journal: Nature Medicine [Category] SARS, 변종, 유전자 메커니즘, 진단, [키워드] acute respiratory syndrome antibody B.1.1.7 B.1.351 B.1.351 variant B1.351 circulating collected convalescent convalescent individual convalescent sera coronavirus D614G examined gene encoding GFP immune evasion immunized individual individuals infected individuals Infection less Mutation nasal swab natural infection Neutralization assay neutralized neutralizing antibody neutralizing titers Pfizer positive raise reduction in remained reporter cell risk of infection SARS-CoV-2 SARS-CoV-2 antibody SARS-CoV-2 B.1.1.7 SARS-CoV-2 variant sensitivity sera Serological assay South Africa Spike protein spread to strain Symptoms tested the spike protein United Kingdom vaccinated individual vaccination vaccine dose vaccinees variant virus [DOI] 10.1038/s41591-021-01318-5 [Article Type] Research
Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteinsArticle Published on 2021-03-192022-10-31 Journal: Communications Biology [Category] Coronavirus, SARS, [키워드] Affect Analysis Chromatography develop expressed expression false-negative False-positive FSEC functional fused GFP High-throughput screening Jin membrane membrane protein Membrane proteins mitigate nanobody peptide powerful tool Proteins purification stability structural biology Topology [DOI] 10.1038/s42003-021-01891-y PMC 바로가기 [Article Type] Article