Comprehensive mapping of SARS-CoV-2 interactions in vivo reveals functional virus-host interactions생체 내 SARS-CoV-2 상호 작용의 포괄적 인 매핑은 기능적 바이러스-숙주 상호 작용을 나타냅니다Article Published on 2021-08-252022-09-10 Journal: Nature Communications [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] approach bind Cell cells cellular Comprehensive element epigenomics functional Genome global health Health Host host mRNA identify Illumina in viral in vivo Interaction interactions Methylation mitochondrial molecular mutant observé pathogenicity platform regions report reveal RNA RNA sequencing RNA-RNA interaction RNA-RNA interactions RNAs SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 viral secondary structure Sequencing small nucleolar RNA structures subgenomic RNA subgenomic RNAs targeted therapy the SARS-CoV-2 genome untranslated regions Viral viral genome virus virus genome virus infection Virus-host interaction Virus-host interactions wildtype Δ382 [DOI] 10.1038/s41467-021-25357-1 PMC 바로가기 [Article Type] Article
SARS-CoV-2 viral proteins NSP1 and NSP13 inhibit interferon activation through distinct mechanismsSARS-COV-2 바이러스 단백질 NSP1 및 NSP13은 별개의 메커니즘을 통해 인터페론 활성화를 억제합니다.Research Article Published on 2021-06-242022-08-13 Journal: PLoS ONE [Category] Biochemistry, MERS, SARS, 유전자 메커니즘, 진단, [키워드] Activation acute respiratory syndrome acute respiratory syndrome coronavirus bind bypasse caused coronavirus Disease progression downregulate global pandemic host mRNA identify IFIT1 immune evasion infecting inhibit innate immune activation interferon interferon signaling IRF3 Last mechanism mechanisms molecular mechanism molecular mechanisms multiple innate immune activation pathways NF-κB NF-κB activation NF-κB phosphorylation NF-κB promoter signaling nsp1 nsp13 nuclear translocation pathway phospho-IRF3 phospho-TBK1 Protein protein expression protein level reduce reduced respiratory SARS-CoV-2 SARS-CoV-2 pathogenesis SARS-CoV-2 viral severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 TBK1 the SARS-CoV-2 These data translation Viral protein Viral proteins virus infection [DOI] 10.1371/journal.pone.0253089 PMC 바로가기 [Article Type] Research Article
Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1Research Article Published on 2021-06-162022-10-30 Journal: Microbiology Spectrum [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Basis binding C terminus coronavirus crystal structure fluorescent full-length function functional fusing host mRNA host protein inhibit inhibitory mRNA N terminus N-terminal nonspecific nsp1 pathogenic Protein protein translation restored ribosome ribosomes SARS-CoV SARS-CoV-2 significantly translation weakened [DOI] 10.1128/Spectrum.00169-21 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 uses a multipronged strategy to impede host protein synthesis Research Published on 2021-05-122022-10-05 Journal: Nature [Category] SARS, 유전자 메커니즘, [키워드] accelerated cellular cellular mRNAs coronavirus SARS-CoV-2 cytosolic Degradation Effect facilitate Gene Expression Host host mRNA host protein impairment implicated infected cells Infection innate immune innate immune genes mechanism mRNA mRNA export nuclear pandemic Prevent Protein protein synthesis reduction in repress ribosomes RNA sequencing SARS-CoV-2 SARS-COV-2 infection suppress synthesized RNA transcribed transcript translated translation variety viral transcript [DOI] 10.1038/s41586-021-03610-3 [Article Type] Research
SARS-CoV-2 ORF6 Disrupts Bidirectional Nucleocytoplasmic Transport through Interactions with Rae1 and Nup98Research Article Published on 2021-04-132022-10-29 Journal: mBio [Category] COVID-19, MERS, SARS, [키워드] accessory protein accessory proteins acute respiratory syndrome Amino acid block C terminus contribute coronavirus coronavirus disease COVID-19 cytoplasm disrupt elevated ENCODE expression Factors Genome growth host antiviral responses host cell host mRNA host proteins in vitro inhibitor interact Interaction interferon mock-infected cells mRNA mRNA export mRNAs Mutation nuclear nucleocytoplasmic transport Nup98 ORF6 pandemic pathogenicity potential mechanism presymptomatic transmission Prevent Protein protein-protein interaction Rae1 reduced replicate reporter reporter expression required responsible RNA virus SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-infected cell shown significantly Support symptom onset the SARS-CoV-2 These data transcribed transcript translate Transport Virology virus VSV M while [DOI] 10.1128/mBio.00065-21 PMC 바로가기 [Article Type] Research Article
Targeting the DEAD-Box RNA Helicase eIF4A with Rocaglates—A Pan-Antiviral Strategy for Minimizing the Impact of Future RNA Virus PandemicsReview Published on 2021-03-052022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] Antiviral broad-spectrum antiviral broad-spectrum antivirals candidate caused cellular complex Compound coronavirus COVID-19 CR-31-B drug eIF4A eukaryotic translation exhibiting fraction Future Helicase highlight host mRNA Impact increase in inhibitors inhibitory effect interactions Intervention limit maintaining mechanism of action mRNA outbreak pan-antiviral Pandemics protein synthesis Region Replication resulting RNA RNA secondary structure RNA virus RNA viruses rocaglates safety profile sequence Side effects silvestrol Strategy targeting therapeutic translation translation initiation unique Vaccine viral mRNA zoonotic origin Zotatifin [DOI] 10.3390/microorganisms9030540 PMC 바로가기 [Article Type] Review
Mechanisms of Coronavirus Nsp1-Mediated Control of Host and Viral Gene ExpressionReview Published on 2021-02-022022-10-31 Journal: Cells [Category] COVID-19, MERS, [키워드] activity acute respiratory syndrome Amino acid antiviral responses biological function cell interaction cellular cleavage contribute Control Coronaviruses CoVs disrupt endonucleolytic cleavage evade function Gene Expression genomes highly pathogenic Host host gene expression host gene expression suppression host mRNA human coronavirus identity involved mechanism MERS-CoV Middle East mRNA mRNA degradation nonstructural protein 1 nsp1 pathogenic SARS-CoV SARS-CoV-2 suppresse syndrome translation translational suppression viral mRNA viral pathogenicity Viral protein viral replication virulence virulence factor virus virus pathogenesis [DOI] 10.3390/cells10020300 PMC 바로가기 [Article Type] Review
SARS-CoV-2 nsp1: Bioinformatics, Potential Structural and Functional Features, and Implications for Drug/Vaccine DesignsMicrobiology Published on 2020-09-292022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] analyzed Antiviral bioinformatics Bioinformatics analysis caused Characteristics conserved coronavirus coronavirus disease COVID-19 drug drug and vaccine development exhibit functional host mRNA inflammation response Innate immunity non-structural protein notable nsp1 other coronavirus pandemic Pathogenesis pathogenesis and development pathogenic factor Potential Prophylaxis Protein protein sequence reveal SARS-CoV SARS-CoV-2 SARS-CoV-2 strain sequence sequence identity structure and function analysis tested translation Treatment strategies unique Vaccine viral infection Viral protein virulence Virus-host interaction [DOI] 10.3389/fmicb.2020.587317 PMC 바로가기 [Article Type] Microbiology
SARS Coronavirus nsp1 Protein Induces Template-Dependent Endonucleolytic Cleavage of mRNAs: Viral mRNAs Are Resistant to nsp1-Induced RNA CleavageResearch Article Published on 2011-12-082022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] 40S accelerated accompany acute respiratory syndrome Antiviral Author bind binding block cells characterized circumvent classical cleavage cleavage sites coronavirus cricket paralysis viruses Degradation demonstrated different element elements events exhibited facilitate Fever found function Hepatitis Host host gene expression host genes host mRNA host protein in vitro induce infected cells Infection inhibitor leader sequence Modification mRNA mRNAs nucleotide nucleotide sequence Protein protein synthesis recognize reporter Resistant ribosome RNA RNA modification SARS Coronavirus SARS infection severe severe symptom subunit suppress the expression susceptible translation type I type I interferon unique viral mRNA Viral protein virus virus replication [DOI] 10.1371/journal.ppat.1002433 PMC 바로가기 [Article Type] Research Article