immunofluorescence assay – Page 2 – Communicable Diseases Research

In-House Immunofluorescence Assay for Detection of SARS-CoV-2 Antigens in Cells from Nasopharyngeal Swabs as a Diagnostic Method for COVID-19

Article

Published on 2021-12-132022-10-31
Journal: Diagnostics
[Category] COVID-19,

Evaluation of different platforms for the detection of anti-SARS coronavirus-2 antibodies, Thailand

항 -SARS 코로나 바이러스 2 항체의 검출을위한 다른 플랫폼의 평가, 태국

Research

Published on 2021-12-062022-09-01
Journal: BMC Infectious Diseases
[Category] SARS, 진단, 치료기술,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 analysed analysis anti-SARS-CoV-2 anti-SARS-CoV-2 antibody antibodies antibody Antibody detection Architect assays Asymptomatic Chemiluminescence assay comparable coronavirus Coronavirus-2 country COVID-19 detect determine Disease diagnosis ELISA evaluated False-positive results feasibility four group four groups help IgG immunofluorescence assay immunological indirect immunofluorescence individuals Infection infection rate Laboratory large sample microneutralization Microneutralization assay Negative predictive value platform positive Positive predictive value Predictive value Prevalence respiratory Result risk SARS-coronavirus-2 SARS-CoV-2 SARS-CoV-2 antibodies sensitivity sera serum serum samples severe acute respiratory syndrome Coronavirus specificity symptomatic total Ig Vaccines
[DOI] 10.1186/s12879-021-06921-y PMC 바로가기 [Article Type] Research

D-dimer as a biomarker for assessment of COVID-19 prognosis: D-dimer levels on admission and its role in predicting disease outcome in hospitalized patients with COVID-19

COVID-19 예후 평가를위한 바이오 마커로서의 D-Dimer : COVID-19를 가진 입원 환자에서 입원 및 질병 결과를 예측하는 데있어서 D-Dimer 수준.

Research Article

Published on 2021-08-262022-08-13
Journal: PLoS ONE
[Category] Biochemistry, MERS, SARS, 바이오마커,

[키워드] 95% CI Accuracy adjusted Admission age an area analyzed Biomarker calculate characteristic clinical confirmed COVID-19 case coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 case COVID-19 patients Cox proportional hazard Cox proportional hazards regression analysis Cutoff cutoff value D-dimer D-dimer level D-dimer value determine died dimer disease Elevation fibrinogen Fibrinogen Equivalent Unit hazard ratio hospital Hospital mortality Hospital stay hospitalized patient hospitals in Kathmandu immunofluorescence assay laboratory parameter Laboratory parameters Mortality on admission outcome Patient patients with COVID-19 poor prognosis predict Predictive Predictive value Prognosis receiver operating characteristic receiver operating characteristic curve recorded regression analysis reported respiratory Respiratory illness Result ROC Curve sensitivity specificity surviving patient surviving patients thrombotic disorders two group two groups was measured was used with COVID-19
[DOI] 10.1371/journal.pone.0256744 PMC 바로가기 [Article Type] Research Article

Remdesivir and Cyclosporine Synergistically Inhibit the Human Coronaviruses OC43 and SARS-CoV-2

Pharmacology

Published on 2021-08-132022-10-31
Journal: Frontiers in Pharmacology
[Category] COVID-19,

[키워드] Cell Combination COVID-19 cyclosporine Cytokine storm drug fibroblast HCoV-OC43 HCT-8 Human human coronavirus OC43 human lung IFA IL-6 IL-8 immunofluorescence assay inhibited inhibitor inhibitory activity OC43 reduced Remdesivir SARS-CoV-2 synergistic treatment for COVID-19
[DOI] 10.3389/fphar.2021.706901 PMC 바로가기 [Article Type] Pharmacology

Highly feasible immunoprotective multicistronic SARS-CoV-2 vaccine candidate blending novel eukaryotic expression and Salmonella bactofection

새로운 진핵생물 발현과 살모넬라 박토펙션을 혼합한 매우 실현 가능한 면역보호 멀티시스트론 SARS-CoV-2 백신 후보

Article

Published on 2021-07-222022-09-11
Journal: Journal of advanced research
[Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단,

[키워드] Analysis anti-SARS-CoV-2 immune responses Antibody titers antigen-specific antibody Antiviral CD4 CD8 Cell-mediated immunity Cellular immune response co-expression COVID-19 Cytometry demonstrated determine develop DNA domain effective Efficacy Efficiency elicited epitope Epitopes eukaryotic expressing expression feasible Flow flow cytometry Frame fused gene delivery heptad repeat highest humoral immune responses immunized mice immunofluorescence assay immunogenicity IMPROVE in vivo increase in investigated mechanism membrane protein mice mRNA Multicistronic expression multivalent vaccine neutralizing antibody titers nsp13 offer Open reading frame pandemic peptide polarization Protein protein expression raised RBD recall Receptor-binding domain recorded RNA robust Safe Salmonella SARS-CoV-2 SARS-CoV-2 gene SARS-CoV-2 genes SARS-CoV-2 neutralizing antibody SARS-CoV-2 protein SARS-CoV-2 proteins SARS-CoV-2 vaccine SARS-CoV-2 variant SARS-CoV-2 variants sera Spike protein splenocyte splenocytes suggested T cell T cells targets Th1 Th1 response Th2 immunity the spike protein the vaccine Vaccine vaccine candidate vaccine construct variant Variant. variants were used western blot Western blot analysis
[DOI] 10.1016/j.jare.2021.07.007 PMC 바로가기 [Article Type] Article

SARS-CoV-2 Infection Remodels the Phenotype and Promotes Angiogenesis of Primary Human Lung Endothelial Cells

Article

Published on 2021-07-032022-10-29
Journal: Microorganisms
[Category] COVID-19,

[키워드] absence ACE2 expression acute lung injury acute respiratory distress analyses Analysis Angiogenesis angiogenic response antiviral molecule ARDS conditioned COVID-19 Cytopathic effect detectable early phase Endothelial cell endothelial cell dysfunction expression Human Hypothesis identify immunofluorescence assay infect Infection Inflammation Inflammatory inhibit integrin Intussusceptive angiogenesis life-threatening lung manifestation Microarray microenvironment MX1 Particles pathogenic mechanism phenotype plethora potential therapeutic target pro-inflammatory proteome Respiratory complications SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection stimulated Support syndrome vascular dysfunction vascular leakage viral infection
[DOI] 10.3390/microorganisms9071438 PMC 바로가기 [Article Type] Article

Broad Severe Acute Respiratory Syndrome Coronavirus 2 Cell Tropism and Immunopathology in Lung Tissues From Fatal Coronavirus Disease 2019

광범위한 중증 급성 호흡기 증후군 코로나바이러스 2 치명적인 코로나바이러스 질병 2019에서 폐 조직의 세포 친화성 및 면역병리학

Article

Published on 2021-06-042022-09-11
Journal: The Journal of Infectious Diseases
[Category] MERS, SARS, 유전자 메커니즘, 진단,

[키워드] ACE2 ACE2-positive cell Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adaptive alveolar alveolar damage angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 Autopsy B cell B cells Cell cell tropism cells ciliated contributing to coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 Cytotoxic T cells DAD Depletion Diffuse alveolar damage endothelial cells excessive inflammation exhibited expression fatal COVID-19 goblet IL-6 immune cells immune responses immunofluorescence immunofluorescence assay immunohistochemistry Immunosuppression infected cell infected cells Infection infiltrating immune cell infiltrating immune cells Inflammation inflammatory cell inflammatory cells Innate immune cells interleukin-6 Lineage lung lung parenchyma Lungs macrophages marker mechanism Monocytes MOST natural killer Neutrophils NK cells Patient performed positive Protein pulmonary symptom pulmonary symptoms reflected regulatory T cells respiratory SARS-CoV-2 SARS-CoV-2 cell severe acute respiratory syndrome Coronavirus staining T cell T cells T helper cells thromboemboli. Thromboembolism tissue tissue damage to define tropism Viral viral infection Viral protein Viral proteins viral tropism
[DOI] 10.1093/infdis/jiab195 PMC 바로가기 [Article Type] Article

Shedding of infectious SARS-CoV-2 by hospitalized COVID-19 patients in relation to serum antibody responses

혈청 항체 반응과 관련하여 입원한 COVID-19 환자에 의한 감염성 SARS-CoV-2의 배출

Research

Published on 2021-05-272022-09-01
Journal: BMC Infectious Diseases
[Category] SARS, 진단, 치료기술,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 airway airways antibodies Antibody Response Antibody titers caused coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient cross-sectional cross-sectional study Culture detectable global pandemic Hospitalized hospitalized COVID-19 patient hospitalized COVID-19 patients IgG immunofluorescence assay immunofluorescence assay (IFA Immunoglobulin Immunoglobulin G in vitro Karolinska University Hospital lower risk median microneutralization Microneutralization assay Neutralizing antibodies neutralizing antibodies titer neutralizing antibodies titers Neutralizing antibody titer neutralizing antibody titers objective Patient patients PCR analysis performed positively correlated potential risk replicating virus respiratory Result SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 neutralizing antibodies SARS-CoV-2 neutralizing antibody SARS-CoV-2 RNA SARS-CoV-2 specific antibodies SARS-CoV-2 transmission SARS-CoV-2-specific IgG serum serum antibody response severe acute respiratory syndrome Coronavirus shedding specific antibody symptom duration symptom onset Total Transmission two patients Viral viral shedding virus were assessed
[DOI] 10.1186/s12879-021-06202-8 PMC 바로가기 [Article Type] Research

Maintenance of neutralizing antibodies over ten months in convalescent SARS‐CoV‐2 afflicted patients

회복기 SARS-CoV-2 환자에서 중화항체를 10개월 이상 유지

Original Article

Published on 2021-05-272022-09-01
Journal: Transboundary and emerging diseases
[Category] COVID19(2023년), MERS, SARS, 진단,

A Novel Immunofluorescence Assay for the Rapid Serological Detection of SARS-CoV-2 Infection

SARS-CoV-2 감염의 신속한 혈청학적 검출을 위한 새로운 면역형광 분석법

Article

Published on 2021-04-242022-09-01
Journal: Viruses
[Category] MERS, SARS, 변종, 진단, 치료기술,