Development and comparison of enzyme-linked immunosorbent assays based on recombinant trimeric full-length and truncated spike proteins for detecting antibodies against porcine epidemic diarrhea virusResearch Article Published on 2019-11-272022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] antibody caused Cell cell line development disease Efficacy ELISA ELISA kit ELISAs embryonic kidney enzyme-linked immunosorbent assay epidemic diarrhea exhibited expressed expression full-length full-length S Genotype high mortality Human embryonic kidney (HEK)-293 mammalian cell expression system humoral hyperimmune immunization immunostaining Impact indirect ELISA infected cell mucosal immune Neonatal nucleocapsid outbreak PEDV Porcine epidemic diarrhea (PED) Protein Result sensitivity Sensitivity and specificity sera Serological assay serological test serology specificity Spike protein Spike protein specific ELISA trimeric virus was selected [DOI] 10.1186/s12917-019-2171-7 PMC 바로가기 [Article Type] Research Article
Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitisResearch Published on 2018-12-122022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] amplified analysed antibody Antigen association bronchitis cDNA Cell Chromatography coding region coding sequence Combined combined use competent Concentration confidence interval controls demonstrated detect develop Diagnosis E. coli early diagnosis ELISA Escherichia coli evaluate examined expected expressed expression vector GE Healthcare GraphPad Prism GraphPad software greater IBV indirect ELISA Infectious Bronchitis Virus Invitrogen liquid molecular N protein nucleocapsid protein nucleoprotein PCR positive powerful tool Prevent primer Protein purification purified receiver-operating characteristics curves recombinant antigen required Result RT-PCR scored SDS-PAGE sensitivity sera serum dilution serum sample specificity subunit Test tested the disease the N protein USA was obtained were used Western blot analysis window yielding [DOI] 10.1186/s12985-018-1096-2 PMC 바로가기 [Article Type] Research
Detection of Salmonella Infection in Chickens by an Indirect Enzyme-Linked Immunosorbent Assay Based on Presence of PagC Antibodies in Seraarticle Published on 2018-02-282024-09-01 Journal: Foodborne Pathogens and Disease [Category] 살모넬라증, [키워드] Antibody detection indirect ELISA PagC Salmonella [Article Type] article