Flipped over U: structural basis for dsRNA cleavage by the SARS-CoV-2 endoribonuclease뒤집힌 U: SARS-CoV-2 엔도리보뉴클레아제에 의한 dsRNA 절단의 구조적 기초Article Published on 2022-08-122022-09-11 Journal: Nucleic Acids Research [Category] SARS, 유전자 메커니즘, 치료법, [키워드] activate Activation active site cleavage cleave coronavirus Coronavirus replication Critical cryo-EM structure cryo-EM structures double-stranded dsRNA endoribonuclease evade form generate Host immune MDA5 mechanism Nsp15 Pattern recognition pattern recognition receptor platform processing recognize replication-transcription complex RNA SARS-CoV-2 SARS-CoV-2 Nsp15 single-stranded site-directed mutagenesis structures substrates the SARS-CoV-2 uridine Vesicle viral replication [DOI] 10.1093/nar/gkac589 PMC 바로가기 [Article Type] Article
Sequence-Specific Features of Short Double-Strand, Blunt-End RNAs Have RIG-I- and Type 1 Interferon-Dependent or -Independent Anti-Viral Effects짧은 이중 가닥, 평활 말단 RNA의 서열 특이적 특징은 RIG-I 및 유형 1 인터페론 의존성 또는 독립적 항바이러스 효과를 갖는다Article Published on 2022-06-282022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료법, 치료제, [키워드] adaptors Adenovirus Anti-viral anti-viral effect Antiviral Antiviral agents Antiviral effect antiviral effects assays Cell cell cycle cell cycle genes cell line cells cellular complementary cytoplasmic DNA DNA virus downregulated dsRNA dsRNAs Effect elicited fibroblast Gene Expression H3N2 human airway IFN IFN-alpha IFN-I IFNAR2 independent independent of indicated Infection Influenza influenza A influenza A virus Influenza virus interferon knock-out library MDA5 mechanism mimicking molecular patterns MYD88 organism Organisms pathogen pathogen-associated molecular patterns Pathogens Randomized receptor required retinoic acid retinoic acid-inducible gene I RIG-I RNA RNA therapy RNA virus RNAs SARS-CoV-2 sequence short double-strand blunt-end RNA. transcriptomics transfection Type ZNFX1 [DOI] 10.3390/v14071407 PMC 바로가기 [Article Type] Article
Anti-MDA5 Antibody Linking COVID-19, Type I Interferon, and Autoimmunity: A Case Report and Systematic Literature ReviewCOVID-19, I형 인터페론 및 자가면역을 연결하는 항MDA5 항체: 증례 보고 및 체계적인 문헌 검토Case Reports Published on 2022-06-272022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 바이오마커, [키워드] Activation anti-MDA5 syndrome antibody antiviral role Autoimmune Autoimmune disease Autoimmune diseases Cardiomyopathy Clinical features constitutional symptoms contribute correlated COVID-19 COVID-19 severity Cytokines differentiation antigen disease Disease activity elevated example explain Features IFN immune immune response immunological immunology in some induce Inflammatory inflammatory myositis interferons literature lung involvement Manifestations MDA5 mechanisms Melanoma myositis Patient Pattern recognition pattern recognition receptor protective effect Protective effects recognizing report review SARS-CoV-2 infected patients SARS-COV-2 infection searched severity signature similarities similarity skin manifestations Symptoms syndrome synthesis Trigger Type Type I IFN type I interferon type I interferon signature. type I interferons Viral viral infections virus viruses while with COVID-19 [DOI] 10.3389/fimmu.2022.937667 PMC 바로가기 [Article Type] Case Reports
Severe Acute Respiratory Syndrome Coronavirus 2 ORF8 Protein Inhibits Type I Interferon Production by Targeting HSP90B1 Signaling중증 급성 호흡기 증후군 코로나바이러스 2 ORF8 단백질은 HSP90B1 신호 전달을 표적으로 하여 I형 인터페론 생산을 억제합니다Article Published on 2022-05-232022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Amino acid Antiviral caused coronavirus coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID-19 exhibit explanation function Genotype genotypes global pandemic high mutation rate high mutation rates HSP90B1 IFN-I IFN-β immune evasion Immune suppression implication individual induce inhibit inhibitory effect interact interferon interferon response investigated IRF3 ISG15 leucine MDA5 mechanism Mutation no difference nuclear translocation ORF8 ORF8 protein pandemic pathogenic pathway poly(I:C) Production Protein provide reported respiratory RIG-I SARS-CoV-2 Serine severe acute respiratory syndrome Coronavirus signaling pathway strain suppress targeting the SARS-CoV-2 Treatment Type type I interferon variant [DOI] 10.3389/fcimb.2022.899546 PMC 바로가기 [Article Type] Article
Virus-specific editing identification approach reveals the landscape of A-to-I editing and its impacts on SARS-CoV-2 characteristics and evolution바이러스 특정 편집 식별 접근 방식은 A-to-I 편집 환경과 SARS-CoV-2 특성 및 진화에 미치는 영향을 보여줍니다.Article Published on 2022-03-212022-09-11 Journal: Nucleic Acids Research [Category] SARS, 변종, 유전자 메커니즘, 치료법, [키워드] accelerated activate Affect antigenicity Antiviral approach atlas average cell types Characteristics component components correlated dsRNA event Evidence Evolution expression framework Genome highlight Host hotspot Human IFN response Impact induce infected with SARS-CoV-2 innate immune response intensity MDA5 provide provided regulated resource reveal RNA RNA virus RNA-seq data SARS-CoV-2 SARS-CoV-2 evolution SARS-COV-2 infection spike gene the epidemic tissue viral dsRNA viral infectivity Viral RNA [DOI] 10.1093/nar/gkac120 PMC 바로가기 [Article Type] Article
Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine Research Published on 2022-03-142022-10-05 Journal: Nature Immunology [Category] 치료제, [키워드] adaptive analyzed antibody BNT162b2 BNT162b2 mRNA vaccine BNT162b2 vaccine CD8+ T cell cell death pathways circulating dependent on draining lymph node Efficacy IFN-γ immune responses Immunity immunization immunological mechanism inflammasome activation innate response interferon knockout mice MDA5 mice molecular mechanism natural killer cell necroptosis Pfizer-BioNTech pyroptosis response serum Signaling stimulate stimulated T cell response T cell responses Toll-like receptor type I underlie [DOI] 10.1038/s41590-022-01163-9 [Article Type] Research
All-Trans Retinoic Acid Attenuates Transmissible Gastroenteritis Virus-Induced Inflammation in IPEC-J2 Cells via Suppressing the RLRs/NF‐κB Signaling PathwayImmunology Published on 2022-01-312022-11-01 Journal: Frontiers in Immunology [Category] Coronavirus, [키워드] acid Activation all-trans retinoic acid anti-inflammatory activity ATRA attenuate Cell cells damaged downregulated downstream economic loss epithelial epithelial cell gastroenteritis IL-1β IL-6 IL-8 immunomodulatory properties indicated Infection Inflammation Inflammatory Inflammatory cytokine Inflammatory cytokines Inflammatory response inhibiting inhibitor Injury intestinal intestinal inflammation IPEC-J2 cells damage knockdown MAVS MDA5 mechanism molecular mechanism mRNA abundance mRNA expression NF-κB nuclear translocation occludin p65 Pathogenesis Phosphorylation prevented pro-inflammatory cytokines protective effect protein level reduced reduction resulting reversed RIG-I RLRs/NF‐κB pathway signaling molecule signaling pathway significantly Suppressing TGEV TLR3 TLR7 TNF-α Traf6 transmissible gastroenteritis virus Treatment TRIF triggered upregulation ZO-1 [DOI] 10.3389/fimmu.2022.734171 PMC 바로가기 [Article Type] Immunology
N7-Methylation of the Coronavirus RNA Cap Is Required for Maximal Virulence by Preventing Innate Immune RecognitionResearch Article Published on 2022-01-252022-10-29 Journal: mBio [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] ACE2 acute respiratory syndrome Brain CAP caused comparable Complete contribute coronavirus coronavirus disease CoV COVID-19 disease effective vaccine exhibited expression guanine IFN-I immune response immunological in vitro Infection Innate interferons ISG15 liver MDA5 MHV mice morbidity and mortality mouse hepatitis virus mutant mutant virus Mutation myeloid cell N7-methylation nonstructural protein 14 nonstructural protein 14 (nsp14) nsp14 pandemic Pathogenesis phenotype Prevent Preventing previous study Recognition reduced replicated required restored RNA RNA cap structure RNA methylation SARS-CoV-2 SARS-COV-2 infection Signaling significantly subsequent the vaccine therapy transgenic mice type I interferon understanding upregulated viral replication Viral RNA virulence wild-type [DOI] 10.1128/mbio.03662-21 PMC 바로가기 [Article Type] Research Article
Effects of IFIH1 rs1990760 variants on systemic inflammation and outcome in critically ill COVID-19 patients in an observational translational study관찰 중개 연구에서 중환자 COVID-19 환자의 전신 염증 및 결과에 대한 IFIH1 rs1990760 변이체의 효과Research Article Published on 2022-01-212022-09-10 Journal: eLife [Category] 변종, [키워드] 95% CI 95% confidence interval analyzed Anti-inflammatory anti-inflammatory phenotype applied benefit Blood cause Cell changes in clinical trial clinical trials coding Cohort Concentration Corticosteroid corticosteroid treatment Corticosteroids COVID COVID-19 COVID-19 patient COVID-19 patients Critically ill death determine Dexamethasone discharge distribution drug Effect ENCODE experiment expression Factor funding Gene Expression greater hazard ratio healthy volunteer healthy volunteers help hospital host response Human ICU IFIH1 IL-6 IL6 immune Immune cell immune cells immune system Impact in-silico increase in individual Infection Inflammation Inflammatory response intensive care intensive care unit Laboratory less lower expression Lung injury MDA5 mechanical ventilation mediators moderate to severe modulate mononuclear cell mononuclear cells Mortality outcome outcomes Pathways Patient patients patients with moderate peripheral Peripheral blood Peripheral blood mononuclear cells pharmacogenomics physician populations pro-inflammatory mediators prospectively studied Protein recognize Red reduce regulate respond risk RNA serum Serum IL-6 severe COVID-19 Spain steroid Steroids supported survived systemic inflammation team the patient translational treat treated Treatment triggered variant variants Variation Viral viral infections virus viruses were measured [DOI] 10.7554/eLife.73012 PMC 바로가기 [Article Type] Research Article
COVID-19 Disease and Dermatomyositis: A Mini-ReviewImmunology Published on 2022-01-132022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, [키워드] caused clinical feature clinical manifestation coronavirus disease COVID-19 COVID-19 patient deaths dermatomyositis develop effective high mortality immune system immunosuppressive drug induce Inflammatory cytokines interstitial Interstitial lung disease MDA5 pandemic Pathogenesis poor prognosis PROTECT pulmonary disease reported SARS-CoV-2 suppression systemic inflammatory response therapeutic effect Treatment [DOI] 10.3389/fimmu.2021.747116 PMC 바로가기 [Article Type] Immunology