Hydrothermal assisted biogenic synthesis of silver nanoparticles: A potential study on virulent candida isolates from COVID-19 patientsArticle Published on 2022-10-062022-11-15 Journal: PLoS ONE [Category] 진단, [키워드] atomic biological activity Candida Candida albican Candida albicans Cell cell membrane coated COVID-19 COVID-19 patient effective Fourier transform High-resolution Ion isolate mechanism Microscopy Modification Morphological PCR question reduction silver nanoparticle TEM Testing thymus Till transmission electron microscopy virulence virulent vulgaris [DOI] 10.1371/journal.pone.0269864 PMC 바로가기
SARS-CoV-2 spike protein aggregation is triggered by bacterial lipopolysaccharideArticle Published on 2022-10-012022-11-16 Journal: Febs Letters [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] aggregate aggregation analyses assays bacterial lipopolysaccharide binding boost COVID-19 demonstrated endotoxins Evidence gel High dose Inflammation Invasion lipopolysaccharide LPS Microscopy Molecular simulation oligomers peptide predicted Protein protein-aggregation Proteins S protein SARS-CoV-2 SARS-CoV-2 spike protein sequence Spike protein spike protein. triggered virus [DOI] 10.1002/1873-3468.14490 PMC 바로가기
Molecular Basis of Mink ACE2 Binding to SARS-CoV-2 and Its Mink-Derived VariantsArticle Published on 2022-09-142022-11-15 Journal: Journal of Virology [Category] SARS, 변종, 유전자 메커니즘, 진단, [키워드] ACE2 acute respiratory syndrome addition angiotensin-converting enzyme 2 Basis binding binding affinity binding mechanism changes in complexes coronavirus Cross-species transmission cryo-EM cryo-EM structure demonstrated examined expressing F486L facilitate highlight hosts Human human cell human monoclonal antibodies humans identify indicated Infection information Interaction mechanism Microscopy mink molecular mechanism Mutation N501T neutralized prevented Protein pseudovirus RBD RBD variant RBD variants receptor reduced residue SARS-CoV-2 SARS-CoV-2 variant sera Structure the binding affinity the RBD the receptor-binding domain the SARS-CoV-2 transmitted variant variants virus entry Virus entry. viruses Y453F [DOI] 10.1128/jvi.00814-22 PMC 바로가기
Molecular Analyses of Clinical Isolates and Recombinant SARS-CoV-2 Carrying B.1 and B.1.617.2 Spike Mutations Suggest a Potential Role of Non-Spike Mutations in Infection KineticsArticle Published on 2022-09-122022-11-15 Journal: Viruses [Category] SARS, 변종, 진단, [키워드] acute respiratory syndrome addition analysis B.1 B.1.617.2 BAC BAC mutagenesis backbone Bacterial bronchial epithelial cells Carrying chromosome clinical comparable coronavirus Cytopathic effect disease Efficiency en passant mutagenesis epithelial cell line evaluate generate glycoprotein human lung Infection infectious virus inhibitors isolate isolates Kinetics less Microscopy monoclonal antibody Mutagenesis Mutation neutralization nonstructural proteins nucleocapsid Numerous Post-vaccine Potential progression raise recombinant RNA role SARS-CoV-2 sera severity spike spike mutation spike mutations Spike protein spike protein. Substitution suggested susceptible Syncytia Transmissibility Treatment variant viral fitness viral spike virus Virus life cycle VoC [DOI] 10.3390/v14092017 PMC 바로가기
SARS-CoV-2 Production, Purification Methods and UV Inactivation for Proteomics and Structural StudiesArticle Published on 2022-09-082022-11-16 Journal: Viruses [Category] SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome biosafety can be used cell activation conditions core Coronavirus-2 COVID-19 died downstream effective experiments faster handling inactivated inactivation Laboratory Microscopy pandemic pathogenicity Production purification resulting SARS-CoV-2 Sequencing Seven significantly size exclusion structural studies. sucrose cushion ultracentrifugation UV inactivation virus [DOI] 10.3390/v14091989 PMC 바로가기
Stem cells, cell therapies, and bioengineering in lung biology and disease 2021Article Published on 2022-09-012022-11-15 Journal: American journal of physiology. Lung cellular and [Category] 진단, [키워드] airway Analysis biennial bioengineering Biology Cell cells cellular college COVID-19 pandemic Cystic differentiation disease Extracellular Extracellular matrix feature foundation heart human lung lung Lung diseases lung regeneration lung tissues Medicine Microscopy plasticity pluripotent stem cells pluripotent stem cells. proteomics recommendation Regulation remained Research Signaling Society STEM stem cell Therapies translational Treatment University of Vermont viral infection [DOI] 10.1152/ajplung.00113.2022 PMC 바로가기
Myopathy as a cause of fatigue in long-term post-COVID-19 symptoms: Evidence of skeletal muscle histopathology코로나19 이후 장기 증상에서 피로의 원인인 근병증: 골격근 조직병리학의 증거Article Published on 2022-09-012022-09-11 Journal: European journal of neurology [Category] COVID19(2023년), SARS, 변종, [키워드] ABC acute disease acute infection affected age analyzed Antigen basal lamina biopsy Capillaries capillary cells changes changes in COVID-19 symptoms Cytochrome C cytochrome c oxidase diagnosed disease electromyography electron microscopy evidence of expression fatigue fiber histological change histological changes histopathological Histopathology human leukocyte antigen indication indications Inflammation Injury less leukocyte long-term COVID Microscopy Mild mitochondrial Most patients muscle muscle biopsy muscle fiber muscle weakness muscular myalgia myopathic Myopathy Patient patients post-COVID syndrome. Quantitative reduced reported SARS-CoV-2 Skeletal muscle skeletal muscles Symptoms syndrome T lymphocyte T lymphocytes two patients variant variety weakness [DOI] 10.1111/ene.15435 PMC 바로가기 [Article Type] Article
Repurposing vilanterol as a novel potential antifungal for Candida albicans: In-silico & in-vitro approachResearch article Published on 2022-09-012022-10-05 Journal: Medicine in Drug Discovery [Category] 신약개발, [키워드] Acridine orange and Ethidium bromide Alter Amino acids CYSCystine Analysis AO/EBAcridine orange and Ethidium bromide approach binding binding free energy C. albican C. albicans Candida Candida albican carried cause cell membrane chronic Conditions obstructive pulmonary disease Clinical and Laboratory Standards Institute clinical study CLSIClinical and Laboratory Standards Institute complex Computational drug Concentration COPDchronic Conditions obstructive pulmonary disease Cystine demethylase diffusion Dimethyl sulfoxide disrupt DMSODimethyl sulfoxide docking method dose drug drug interaction Drug screening drugs Erg11pLanosterol 14-alpha demethylase FDAThe United States Food and Drug Administration fluorescent fungicidal fungicidal activity glycine GLYGlycine Health High-throughput virtual screening HISHistidine histidine HTVSHigh-throughput virtual screening humans In-vitro infect infections inhibitory concentration initial Interaction lanosterol Lanosterol 14-alpha demethylase MDSMolecular Dynamics Simulations MICMinimum inhibitory concentration Microscopy Minimum inhibitory concentration MMGBSAMolecular mechanics energies with generalized born surface area Molecular dynamics simulation molecular dynamics simulations Molecular mechanics energies with generalized born surface area mortality rate PBSPhosphate buffer saline PDBProtein Data Bank Phenylalanine PHEPhenylalanine Phosphate buffer saline Phosphatidylcholines Picoseconds POPCPhosphatidylcholines Protein Data Bank psPicoseconds repurposing Safe SDStandard deviation Serine SERSerine SPStandard-precision Standard deviation Standard-precision tested The United States Food and Drug Administration tyrosine TYRTyrosine Variable-dielectric surface generalized born model 2.0 VSGB 2.0Variable-dielectric surface generalized born model 2.0 XPXtra-precision Xtra-precision Yeast extract/peptone/dextrose YPDYeast extract/peptone/dextrose [DOI] 10.1016/j.medidd.2022.100137 [Article Type] Research article
Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3 Research Published on 2022-08-302022-10-05 Journal: Nature Communications [Category] 변종, 유전자 메커니즘, 진단, [키워드] atomic binding cleavage Complete complex CRISPR Degradation degrade detect DNA DNA degradation double-stranded dsDNA dynamic elicit Escherichia coli exhibit Genome in vitro mechanism Microscopy multi non-specific NTS nucleic acid PAM Partial priming Protein R-loop recruited Release sequence single-stranded DNA spacer ssDNA Type type I [DOI] 10.1038/s41467-022-32618-0 [Article Type] Research
Repurposing the mitotic machinery to drive cellular elongation and chromatin reorganisation in Plasmodium falciparum gametocytes Research Published on 2022-08-272022-10-05 Journal: Nature Communications [Category] 신약개발, [키워드] cellular chromatin complex components cytoplasmic disrupt driven by Endoplasmic reticulum falciform involved kinetochore protein malaria membrane Microscopy microtubule organizing center microtubule-associated protein mitotic nuclear nuclear envelope nuclear membrane Plasmodium falciparum Prevent Protein Proteins repurposing [DOI] 10.1038/s41467-022-32579-4 [Article Type] Research