SARS-CoV-2 infection and replication kinetics in different human cell types: The role of autophagy, cellular metabolism and ACE2 expressionArticle Published on 2022-11-012022-11-16 Journal: Life sciences [Category] SARS, 유전자 메커니즘, [키워드] A549 ACE2 ACE2 expression angiotensin ATP synthase autophagy BEAS-2B Brain Caco-2 can be used Cell cell line cell lines cellular cellular metabolism COVID-19 COX endothelial energy metabolism evaluated expression level HEK-293 human cell human cell line in vitro intestine intrinsically investigated kidney liver mechanism metabolism Mitochondria mitochondrial metabolism modified cell line molecular mechanism mRNA expression NDUFS2 overexpressed overexpressing overexpression Protein Replication replication kinetics respiratory tract RT-qPCR SARS-COV-2 infection SARS-CoV-2 replication SH-SY5Y susceptibility susceptible cell TMPRSS2 TMPRSS2 expression uninfected cell various tissue viral infection Viral load viral replication were assessed western blot [DOI] 10.1016/j.lfs.2022.120930 PMC 바로가기
Elucidation of the inhibitory activity of ivermectin with host nuclear importin α and several SARS-CoV-2 targetsArticle Published on 2022-11-012022-11-15 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 치료제, [키워드] 3CL pro 3CLpro acting antiviral activity approach binding binding free energy complex COVID-19 dimeric docked complexes docking explained FDA-approved drug FIVE heterodimeric Host in vitro activity inhibit inhibitor inhibitory activity Ivermectin MD simulation molecular molecular docking Molecular dynamics simulation molecular mechanism nuclear previous study protease Proteins reached Replication responsible SARS-CoV-2 SARS-CoV-2 replication shown Structural analysis structural property target translocation variety virus with COVID-19 [DOI] 10.1080/07391102.2021.1911857 PMC 바로가기
Porcine deltacoronavirus E protein induces interleukin-8 production via NF-κB and AP-1 activationArticle Published on 2022-11-012022-11-15 Journal: Veterinary microbiology [Category] SARS, 유전자 메커니즘, 치료제, [키워드] abrogated activated activating Activation acute respiratory syndrome Alpha antiviral immunity AP-1 binding C-terminal domain caused chemokine coronavirus coronavirus disease COVID-19 Cytokine release syndrome deleting demonstrated dependent on domain E E protein expression facilitate help IL-6 IL-8 induce Infection Inflammation inhibitor interleukin-6 IκBα life-threatening Local molecular mechanism motif NF-κB NF-κB pathway nuclear nuclear translocation p65 Pathogenesis PDCoV. Phosphorylation Proinflammatory cytokine promoter protein phosphorylation resulting SARS-CoV-2 Severe case upregulated [DOI] 10.1016/j.vetmic.2022.109553 PMC 바로가기
An explainable model of host genetic interactions linked to COVID-19 severityArticle Published on 2022-10-262022-11-16 Journal: Communications Biology [Category] 변종, 유전자 메커니즘, [키워드] age and gender Analysis classifiers Cohort contributing to covariates COVID-19 COVID-19 severity dataset Factor Features Follow-up Genetic GWAS high accuracy highest Host identify immune pathways immune system increased risk Inflammatory Interaction Italian patients MCC molecular mechanism offer outcome Patient phenotypic predict predicted Predictive screened several variant Severe COVID-19 Infection severity Signaling significantly Stratification stratified Support tested therapeutic intervention Thoracic variant viral infection WES with COVID-19 [DOI] 10.1038/s42003-022-04073-6 PMC 바로가기
Immunological and Pathological Peculiarity of Severe Acute Respiratory Syndrome Coronavirus 2 Beta VariantArticle Published on 2022-10-262022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 진단, [키워드] Activation acute respiratory syndrome Analysis Beta Beta variant blockade cascade cause CCL2 Characteristics Comparative analysis coronavirus coronavirus 2 COVID-19 COVID-19 pandemic CXCR2 cytokine cytokine release death Disease progression early stage evidenced IL-1β immunological induce Infection Inflammatory cytokine investigated K18-hACE2 late stage Lung pathology mice molecular mechanism Mortality neutrophil recruitment outbreak pathological pathological condition pathology phenotype presymptomatic provide reduced respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pathogenesis SARS-CoV-2 variant secreted cytokine Strains the SARS-CoV-2 Transcriptome transcriptomes Transmissibility Treatment variant viral infection weight loss [DOI] 10.1128/spectrum.02371-22 PMC 바로가기
SARS-CoV-2 Diverges from Other Betacoronaviruses in Only Partially Activating the IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in Human Lung-Derived CellsArticle Published on 2022-10-262022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] activate Activation activities activity acute respiratory syndrome betacoronaviruses Cell cellular change coronavirus country Critical death downstream Endoplasmic reticulum enzyme ER stress examined expression of gene functions Gene Expression HCoV-OC43 Host host cell host cells host immune system Human human cell human cells imperative individual induce infect Infection inhibit interact IRE1α pathway less limit MERS-CoV MHV Middle East molecular mechanism mRNA murine OC43 Other other coronavirus pathway Prevent processed Replication required Respiratory Coronavirus respiratory syndrome coronavirus Reticulum RNase SARS-CoV SARS-CoV-2 SARS-COV-2 infection severe respiratory disease signaling pathway similarity splice Spread Stress tested the interferon transcription factor Trigger unfolded protein response UPR Vaccine viral replication virus XBP1 [DOI] 10.1128/mbio.02415-22 PMC 바로가기
On the Recognition of Natural Substrate CTP and Endogenous Inhibitor ddhCTP of SARS-CoV-2 RNA-Dependent RNA Polymerase: A Molecular Dynamics StudyArticle Published on 2022-10-242022-11-15 Journal: Journal of Chemical Information and Modeling [Category] COVID19(2023년), SARS, 치료제, [키워드] absence affecting Antiviral computational method COVID-19 outbreak Cryo-electron microscopy cytidine Dynamics endogenous enzyme help implication in silico information inhibitor inhibitory innate immune response investigated MD simulation mechanism molecular molecular mechanism multiple mutation natural novel coronavirus SARS-CoV-2 nucleotide pandemic Protein RdRP RdRp active site Recognition responsible RNA RNA sequence RNA-dependent RNA polymerase SARS-CoV-2 substrate viral replication virus [DOI] 10.1021/acs.jcim.2c01002 PMC 바로가기
Tools to Alleviate the Drug Resistance in Mycobacterium tuberculosisArticle Published on 2022-10-172022-11-15 Journal: Molecules [Category] 진단, [키워드] acid-fast bacillus Antibiotics Asymptomatic cause caused COVID-19 pandemic death detect diagnosed Diagnosis drug Drug discovery drug resistance drug-resistant estimates exhibiting Factor Frequency highlight Host intrinsically isolate isolates majority MDR molecular mechanism mutations Mycobacterium Mycobacterium tuberculosis Nucleic acid detection pathogen reduced reduction in resistance Treatment treatment regimen Tuberculosis WHO [DOI] 10.3390/molecules27206985 PMC 바로가기
Identification of serum metabolites enhancing inflammatory responses in COVID-19Article Published on 2022-10-012022-11-15 Journal: Science China. Life sciences [Category] COVID19(2023년), SARS, 치료제, [키워드] activate Activation acute respiratory syndrome Administered agmatine alleviate Antiviral caused changed characterized circulating metabolite coronavirus Coronavirus disease 2019 COVID-19 COVID-19 infection COVID-19 patient COVID-19 patients cytokine production drug Effect effector elevated elicit embryonic exhibiting fibroblast identification IFNs IL-6 IL-6 level Inflammation Inflammatory cytokine Inflammatory response interferon regulatory factor interferons IRFs macrophages metabolite metabolites metabolites. metabolome mice molecular mechanism NF-κB NF-κB pathway Proinflammatory cytokine putrescine responsible resulting SARS-CoV-2 serum severity significantly stages strong immune response systemic inflammatory responses Tandem mass spectrometry tested the disease TNF type I interferon underlie wildtype [DOI] 10.1007/s11427-021-2099-7 PMC 바로가기
SARS-CoV-2 and mutation RT-qPCR test positivity correlation with ABO and Rh blood typesSARS-CoV-2 및 돌연변이 RT-qPCR 검사 ABO 및 Rh 혈액형과 양성 상관관계Article Published on 2022-10-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] ABO ABO blood type Alpha Beta Blood blood type Chain Reaction correlation COVID-19 determine examined identify individual Infection molecular molecular mechanism Mutation Patient polymerase chain polymerase chain reaction positive qPCR qPCR test real-time polymerase chain reaction reverse transcription Rh blood type RT-qPCR RT-qPCR test SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 transmission [DOI] 10.1002/jmv.27923 PMC 바로가기 [Article Type] Article