Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronavirusesArticle Published on 2022-10-162022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] acute respiratory syndrome Analysis antiviral drug Biological functions cell entry characterization characterized Coronavirus-2 D614G domains FIVE functional glycosylated help less membrane fusion mutant N-glycosylation O-glycosylation Recombinant spike protein S proteins S1 protein S1 subunit SARS-CoV-2 Sialylation spike Spike protein Spike proteins Vaccine β-coronavirus β-coronavirus. β-CoV β-CoVs [DOI] 10.1016/j.aca.2022.340394 PMC 바로가기
Carbohydrate-binding protein from stinging nettle as fusion inhibitor for SARS-CoV-2 variants of concernArticle Published on 2022-08-302022-11-15 Journal: Frontiers in Cellular and Infection Microbiology [Category] SARS, 변종, 치료제, [키워드] addition Antiviral antiviral activity assays Beta binding block Cell cell cultures cell types cell-cell fusion cells circulating Coronaviruses Deletion Delta demonstrated fusion inhibitor Gamma inhibit inhibited inhibitor interface investigated lectin lung epithelial membrane fusion monomeric mutant N-acetylglucosamine (GlcNAc) N-glycosylation omicron Prevent Protein pseudotyped SARS-CoV-2 remained Replication replication of SARS-CoV-2 S2 subunit SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant Spike protein SPR subsequent surface plasmon resonance tested the spike protein UDA Urtica variant variants of concern variants of SARS-CoV-2 Vero E6 cell viral replication viral spike protein virus entry viruses Wuhan-Hu-1 [DOI] 10.3389/fcimb.2022.989534 PMC 바로가기
Site-specific glycosylation of SARS-CoV-2: Big challenges in mass spectrometry analysisReview Published on 2022-08-012023-07-09 Journal: Proteomics [Category] COVID19(2023년), [키워드] glycoproteomics. N-glycosylation O-glycosylation SARS-CoV-2 glycoprotein [DOI] 10.1002/pmic.202100322 PMC 바로가기 [Article Type] Review
A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19코로나19 환자의 SARS-CoV-2 막관통 단백질에 대한 항체 반응을 평가하기 위해 유세포 분석과 결합된 세포 기반 시스템Article Published on 2022-02-222022-09-11 Journal: STAR protocols [Category] COVID19(2023년), SARS, 변종, [키워드] antibody Antibody Response Antibody responses approach Biology Cell Biology Cell-based Assays Complete Effectiveness evaluate Flow flow cytometry Flow Cytometry/Mass Cytometry glycosylation Health Sciences HEK293T cell HEK293T cells host cells insertion Laboratory membrane membrane protein Microbiology Molecular biology N-glycosylation patient sera patients with COVID-19 powerful tool protocol SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants transmembrane protein Vaccine viral infection [DOI] 10.1016/j.xpro.2022.101229 PMC 바로가기 [Article Type] Article
Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorResearch Article Published on 2021-12-202022-10-31 Journal: eLife [Category] COVID-19, SARS, [키워드] ACE2 angiotensin converting enzyme 2 binding binding affinity catalytic activity clinical test complex COVID-19 Critical deleterious effect effective Engineering enzyme glycosylation host cells Human human ACE2 Interaction Molecular dynamics simulation N-glycan N-glycosylation neutralize notable patients Protein receptor recombinant SARS-CoV-2 site-directed mutagenesis spike spike-ACE2 stability to define Treatment treatment for COVID-19 viral entry Virus neutralization viruses [DOI] 10.7554/eLife.73641 PMC 바로가기 [Article Type] Research Article
N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometryArticle Published on 2021-12-072022-10-29 Journal: Scientific Reports [Category] COVID-19, SARS, [키워드] ACE2 binding acute respiratory syndrome cause change changed characterized conditions coronavirus COVID-19 D614G D614G substitution decrease diagnostic tests distribution glycan glycosylated glycosylation glycosylation site increase in Infectious diseases membrane mutant N-glycosylation profile Protein Proteins proteomics reduction in remained SARS-CoV-2 SARS-CoV-2 spike glycoprotein SARS-CoV-2 variant secreted proteins Spike protein the SARS-CoV-2 therapeutic drug Transmission Vaccines variant virus wild type [DOI] 10.1038/s41598-021-02904-w PMC 바로가기 [Article Type] Article
Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro보존된 N-글리코실화를 표적으로 하는 것은 시험관 내에서 SARS-CoV-2 변이체 감염을 차단합니다Article Published on 2021-12-012022-09-11 Journal: EBioMedicine [Category] SARS, 변종, 진단, [키워드] ACE2 ADC adopted Alpha Anti-spike anti-spike antibody antibodies antibody approach assays association attenuate B.1.1.7 B.1.351 Beta binding block chromatin chromatin immunoprecipitation conserved contributed convalescent sera COVID-19 severity Deglycosylation determined by Effectiveness Efficiency ELISA flow cytometry Genome-wide association study glycosylated glycosylation glycosyltransferase GWAS Host host cell host cells immune response immunoblotting immunofluorescence Impact impair in vitro infected cells Infection inhibiting Interaction investigated involved knockdown luciferase MOST N-glycosylation N-glycosylation site N-linked glycosylation NCBI Neutralizing Neutralizing antibodies neutralizing antibody NF-κB NGI-1 positively correlated promoter Receptor binding domain reducing released retrieved SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2-infected cell SARS-CoV-2-infected cells severity Spike protein Spike proteins STT3A STT3A. targeting Vaccine Vaccines variant variants Viral viral entry viral spike protein viruses was determined with COVID-19 [DOI] 10.1016/j.ebiom.2021.103712 PMC 바로가기 [Article Type] Article
Site-Specific Glycosylation Patterns of the SARS-CoV-2 Spike Protein Derived From Recombinant Protein and Viral WA1 and D614G StrainsChemistry Published on 2021-11-192022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] ACE2 receptor Analysis analyzed Cell cell substrate characterized complex core Critical D614G D614G variant defined domain downstream Eclipse Efficiency glycan glycan shield glycans glycosylated glycosylation glycosylation pattern HEK293 high-mannose Host Host–pathogen interaction hydrophilic in viral Infection Interaction less Local microheterogeneity monomer Mutation N-glycan N-glycosylation N-glycosylation site O-glycosylation Orbitrap pattern predicted produced Protein RBD recent recombinant Recombinant protein reported reveal S1 and S2 S1 domain SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein spike Spike protein Spike proteins strain substrate the SARS-CoV-2 Vero Vero cells Vero E6 virus WA1 WA1 strain [DOI] 10.3389/fchem.2021.767448 PMC 바로가기 [Article Type] Chemistry
SARS-CoV-2 integral membrane proteins shape the serological responses of patients with COVID-19Article Published on 2021-09-292022-10-30 Journal: iScience [Category] COVID-19, [키워드] Abstract acute respiratory syndrome Anti-viral Antibody Response antigenicity binding compose coronavirus D614 variant develop Effectiveness elevated elicit elicited evaluated exhibited host cell host immune system Humoral response immune immune response immunogenic potential immunology Infection insertion Like M protein membrane membrane protein moderate N-glycosylation pandemic patients with COVID-19 patients with SARS-CoV-2 post-translational modification powerful tool Proteins reduced responses SARS-CoV-2 SARS-CoV-2 variant Scientific community serological response severe disease Severe patient transmembrane protein unique Vaccine Viral protein Virology with COVID-19 [DOI] 10.1016/j.isci.2021.103185 PMC 바로가기 [Article Type] Article
A Linkage-specific Sialic Acid Labeling Strategy Reveals Different Site-specific Glycosylation Patterns in SARS-CoV-2 Spike Protein Produced in CHO and HEK Cell SubstratesChemistry Published on 2021-09-242022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] ACE2 acid acute respiratory syndrome amidation ammonium hydroxide C-terminal trimerization motif cell substrate cells Chinese hamster CHO cell complex coronavirus determine diagnostics dimethylamine embryonic kidney EThcD evaluate exhibit Express glycan glycan shield glycoproteomics glycosylated glycosylation HEK cell high-mannose Host host immune system immune system Interaction modify N-glycosylation N-linked glycosylation pattern predicted presenting produced profile Protein reaction receptor recombinant S protein Research reveal S protein SARS-CoV-2 sialic acid site occupancy spike Spike protein Spike proteins Strategy substrate Support suspensions the S protein the SARS-CoV-2 These data transmembrane domain Vaccines viral envelope virus [DOI] 10.3389/fchem.2021.735558 PMC 바로가기 [Article Type] Chemistry