Strong SARS-CoV-2 N-Specific CD8 + T Immunity Induced by Engineered Extracellular Vesicles Associates with Protection from Lethal Infection in Mice강력한 SARS-COV-2 N- 특이 적 CD8 + T 면역에 의해 유도 된 세포 외 소포에 의해 유도 된 면역은 마우스에서 치명적인 감염으로부터 보호됩니다.Article Published on 2022-02-062022-09-10 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 변종, [키워드] airways anti-SARS-CoV-2 vaccine antibody waning Antigen Associate Bronchoalveolar lavage fluid CD8 CD8+ T cell immunity cells characterized Combination described distribution DNA dose Efficacy Efficiency Evidence EVs expected expressing extracellular vesicle Extracellular vesicles fused highest idea immune memory immune response Immunity immunization immunized mice induce induced injection Intramuscular injection lethal lethal doses limitation Localization long-lasting lung Lungs Lymphocytes memory cell mice N-terminus Nef percentage platform prophylactic strategy protection Protein remained SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 antigens SARS-COV-2 infection spleen Strong T cell T cells T lymphocyte T lymphocytes target tissue Vaccines variants vector vectors Viral viral variant Viral variants were infected [DOI] 10.3390/v14020329 PMC 바로가기 [Article Type] Article
Antibodies to the N-Terminal Domain of Angiotensin-Converting Enzyme (ACE2) That Block Its Interaction with SARS-CoV-2 S ProteinSARS-CoV-2 S 단백질과의 상호작용을 차단하는 안지오텐신 전환 효소(ACE2)의 N-말단 도메인에 대한 항체Article Published on 2022-02-012022-09-11 Journal: Doklady. Biochemistry and biophysics [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] ACE2 ACE2 receptor angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 antibody block Cell cell membrane COVID-19 COVID-19 pandemic entry receptor enzyme evaluated Ex vivo human ACE2 in vitro interact Interaction main entry receptor MOST N-terminus New coronavirus peplomers peptides protective antibodies receptor receptor. S protein SARS-CoV-2 SARS-CoV-2 S the S protein Vaccinations virus [DOI] 10.1134/S160767292201001X PMC 바로가기 [Article Type] Article
A vesicular stomatitis virus-based prime-boost vaccination strategy induces potent and protective neutralizing antibodies against SARS-CoV-2수포성 구내염 바이러스 기반 프라임-부스트 백신 접종 전략은 SARS-CoV-2에 대한 강력하고 보호적인 중화 항체를 유도합니다Research Article Published on 2021-12-162022-09-12 Journal: PLoS Pathogens [Category] SARS, 진단, [키워드] Adding avirulent chimeric COVID-19 COVID-19 pandemic COVID-19 vaccine cytoplasmic tail demonstrated derivative develop effective effective vaccine expressed expression form full-length full-length spike protein G protein Genome hallmark Health help highest immune system immunization immunized mice induce infections lung damage majority N-terminus Neutralizing antibodies neutralizing antibody pandemic peptide Prevent Protective protective neutralizing antibody Protein protein expression pseudotype RBD Receptor-binding domain recognize reduced resulting S1 subunit Safe SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 protein SARS-CoV-2 spike proteins signal peptide significantly Spike protein stomatitis strain subsequent T cell T cell responses the receptor-binding domain the S1 subunit transmembrane domain Vaccinating Vaccination strategy Vaccine Vaccines variants of concern vesicular stomatitis vesicular stomatitis virus viral disease Viral diseases VSV [DOI] 10.1371/journal.ppat.1010092 PMC 바로가기 [Article Type] Research Article
Comparison of SARS-CoV-2- and HCoV-Specific T Cell Response Using IFN-γ ELISpotArticle Published on 2021-08-102022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] acute respiratory syndrome addition analysis Cell cellular immunity Cohort convalescent coronavirus coronavirus Coronaviruses cross-reactivity detect detectable ELISPOT Endemic Evidence examined groups help herd immunity human endemic coronavirus IFN-γ ELISPOT Immunity Immunocompromised patients individual individuals Infection N-terminus neutralizing antibody no correlation performed Prevent Previous studies receive response SARS-CoV-2 SARS-CoV-2 vaccination Spearman stimulated T cell T cells T-cell Response the spike protein to define Vaccine Volunteer volunteers [DOI] 10.3390/diagnostics11081439 PMC 바로가기 [Article Type] Article
Antibody Response to SARS-CoV-2 Membrane Protein in Patients of the Acute and Convalescent Phase of COVID-19COVID-19의 급성 및 회복기 환자에서 SARS-CoV-2 막 단백질에 대한 항체 반응Immunology Published on 2021-08-042022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 진단, 치료법, [키워드] acute disease acute phase Amino acid amino acids antibodies antibody antibody reactivity Antibody Response B-cell epitope mapping analyses Complete convalescent phase Course COVID-19 COVID-19 antibody COVID-19 patients disease ELISA epitope Epitopes fusion protein historical control IgM IgM response immunodominant immunodominant epitopes linear epitope longitudinal analysis M protein membrane membrane protein N- and C-termini N-terminus nucleocapsid protein overlap Patient peptide peptides phase plasma samples Protein raised RBD reactivity Recognition response SARS-CoV-2 SARS-CoV-2 protein SARS-CoV-2 proteins Serological assay serological assays Seropositivity significantly lower specific antibodies synthetic peptide the antibody response the SARS-CoV-2 understanding Vaccines [DOI] 10.3389/fimmu.2021.679841 PMC 바로가기 [Article Type] Immunology
Genome-wide analysis of protein–protein interactions and involvement of viral proteins in SARS-CoV-2 replicationResearch Published on 2021-07-222022-11-01 Journal: Cell & Bioscience [Category] COVID-19, [키워드] Acidic amino acids Analysis antiviral drug complex coronavirus protein FIVE function Gene Expression Impact inhibited Interaction Interaction map interactions mammalian molecular mechanism N N protein N-terminus NSP Nsp3 NTD positive Protein–protein interaction protein–protein interactions Proteins provided purified Replication reported reporter Result SARS-CoV SARS-CoV-2 SARS-CoV-2 replication screened significantly supplementary material supported Viral protein viral protein–protein interaction Viral proteins viral replication [DOI] 10.1186/s13578-021-00644-y PMC 바로가기 [Article Type] Research
Binding of SARS-CoV-2 Fusion Peptide to Host Endosome and Plasma MembraneArticle Published on 2021-07-132022-10-29 Journal: The journal of physical chemistry. B [Category] COVID-19, SARS, [키워드] acting bind binding C-terminus cellular membrane cleavage Connected conserved Contact eight element endosome flexible linker function fusion fusion peptide Host host cell host cell receptor human host identify Infection initial insertion MD simulation membrane membrane fusion molecular Multiple N-terminus plasma membranes proceed promote Protein required S1 domain S2′ site SARS-CoV-2 second stability subsequent the SARS-CoV-2 the SARS-CoV-2 virus viral envelope viral membrane viral spike virion virus [DOI] 10.1021/acs.jpcb.1c04176 PMC 바로가기 [Article Type] Article
Fusion Protein of Rotavirus VP6 and SARS-CoV-2 Receptor Binding Domain Induces T Cell ResponsesCommunication Published on 2021-07-022022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] antibodies antigen-presenting cells antigenic B cell B cell epitope BALB/c mice caused Cell cell-mediated immune response conditions conformational COVID-19 cross-reactive delivery platform displaying domain fusion fusion protein hACE2 human Angiotensin-converting enzyme humoral immune response Immunity immunize in vitro investigated long-lasting mRNA N-terminus pandemic platform populations produced RBD receptor respiratory response rotavirus S-specific antibody SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 spike protein T cell T cell epitope T cells Vaccine vaccine candidate Vaccines Viral vector VP6 was used [DOI] 10.3390/vaccines9070733 PMC 바로가기 [Article Type] Communication
Recombinant Human Thymosin Beta-4 Protects against Mouse Coronavirus InfectionResearch Article Published on 2021-04-212022-10-31 Journal: Mediators of Inflammation [Category] COVID-19, MERS, [키워드] acetylated Actin activity acute respiratory syndrome Angiogenesis Anti-inflammatory antioxidant BALB/c conducted coronavirus coronavirus Coronavirus infection COVID-19 CoVs disease evaluate experiment expressed function Host Human human CoV human tissues humans immune response infect inhibiting linear liver MERS MERS-CoV MHV MHV-A59 mice Middle East mouse mouse CoV mouse hepatitis virus N-terminus pathogenic coronavirus peptide promoter protective effect recombinant regulate required RNA genome SARS-CoV SARS-CoV-2 significantly increased survival rate syndrome treat Treatment viral pathogenesis virus replication wound healing [DOI] 10.1155/2021/9979032 PMC 바로가기 [Article Type] Research Article
Modification of N -terminal α-amine of proteins via biomimetic ortho- quinone-mediated oxidationArticle Published on 2021-04-152022-10-04 Journal: Nature Communications [Category] 변종, [키워드] amino acid residues approach assays biological processe Cell chemical modification complementary demonstrated diverse proteins generate inflammatory protein Macrophage Medicinal chemistry MIP-1β Modification Myoglobin N-terminal N-terminus Naturally offer oxidized peptides Protein Proteins reaction reactivity reduction selective SUMO2 unique viability [DOI] 10.1038/s41467-021-22654-7 PMC 바로가기 [Article Type] Article