CTD of SARS-CoV-2 N protein is a cryptic domain for binding ATP and nucleic acid that interplay in modulating phase separationSARS-CoV-2 N 단백질의 CTD는 상분리를 조절하는 데 상호작용하는 ATP 및 핵산 결합을 위한 비밀 도메인입니다Article Published on 2022-02-012022-09-11 Journal: Protein Science : A Publication of the Protein Soc [Category] COVID19(2023년), SARS, 변종, 치료법, [키워드] affinity anti-SARS-CoV-2 ATP ATP: Nucleic acid bind binding binding domain cellular Complete Concentration Critical CTD domain drug target function generate gRNA host cell interface intrinsically life cycle liquid-liquid phase separation (LLPS) living cell modulating MOST N protein NMR NMR spectroscopy NTD nucleic acid nucleocapsid nucleocapsid (N) protein. offer pandemic probe Protein regions representing residue Result SARS-CoV-2 SARS-CoV-2 N protein SARS-CoV-2 nucleocapsid SARS-CoV-2 variants structures target targets the SARS-CoV-2 unique variant viral life cycle virus [DOI] 10.1002/pro.4221 PMC 바로가기 [Article Type] Article
Secondary Structures of the Transmembrane Domain of SARS-CoV-2 Spike Protein in Detergent MicellesArticle Published on 2022-01-182022-10-29 Journal: International Journal of Molecular Sciences [Category] COVID-19, SARS, [키워드] conformation COVID-19 Critical detergent detergent micelles domain form helical structure host cells linker micelle NMR NMR spectroscopy proline Protein Receptor binding Relaxation residue S-protein SARS-CoV-2 secondary secondary structure shown side chain solvent spike Spike protein Structural analysis Structure transmembrane transmembrane domain viral entry viral infectivity [DOI] 10.3390/ijms23031040 PMC 바로가기 [Article Type] Article
NMR‐Based Analysis of Nanobodies to SARS‐CoV‐2 Nsp9 Reveals a Possible Antiviral Strategy Against COVID‐19SARS -COV -2 NSP9에 대한 나노 바디의 NMR 기반 분석Research Article Published on 2021-10-272022-09-10 Journal: Advanced Biology [Category] MERS, SARS, 치료제, [키워드] Against analysis antiviral strategy applied B‐cell characterized complex coronavirus coronavirus disease COVID‐19 COVID‐19 patient COVID‐19 patients COVID‐19 diagnostic epitope Epitopes expressed host cell identify immunoassay immunoassays implication Lineage molecular molecular dynamics monomeric nanobodies nanobody New NMR spectroscopy nonstructural protein nonstructural proteins Nsp9 Nsps nuclear magnetic resonance oligomerization Patient Protein purified recognize Replication required Result RTC Saliva saliva samples SARS‐CoV‐2 SARS‐CoV‐2 Strategy supported therapeutic Transcription unique Viral viral replication [DOI] 10.1002/adbi.202101113 PMC 바로가기 [Article Type] Research Article
Partial structure, dampened mobility, and modest impact of a His tag in the SARS-CoV-2 Nsp2 C-terminal regionOriginal Article Published on 2021-10-112022-10-31 Journal: European Biophysics Journal [Category] COVID-19, SARS, [키워드] assigned atomic level backbone C-terminal cleavage conformational conformations cryo-EM Defense eukaryotic cells flexible Host human protein information interact Interaction Intrinsically disordered proteins molecule N-terminal NMR spectroscopy not affect NSP2 nuclei Partial physiological predicted preference promote Protein Region regulate residues SARS-CoV-2 supplementary material targets the SARS-CoV-2 translation initiation Vesicle viral growth virus while X-ray crystallography [DOI] 10.1007/s00249-021-01575-9 PMC 바로가기 [Article Type] Original Article
The NIH Lipo-COVID Study: A Pilot NMR Investigation of Lipoprotein Subfractions and Other Metabolites in Patients with Severe COVID-19Article Published on 2021-08-262022-10-28 Journal: Biomedicines [Category] COVID-19, [키워드] alteration Atherosclerosis beta-hydroxybutyrate branched-chain amino acid cholesterol complex interplay COVID-19 Diseases elevated evidenced HDL hepatic dysfunction Inflammation intensive care investigation LDL lipids lipoprotein lipoprotein X (LpX), lipoprotein Z (LpZ), GlycA marker metabolites NIH NMR NMR spectroscopy Other Participants Patient patients with COVID-19 pilot study plasma plasma lipoprotein Prognostic markers reduced reported respiratory viral infections SARS-CoV-2 severe COVID-19 triglycerides TRL utility was used [DOI] 10.3390/biomedicines9091090 PMC 바로가기 [Article Type] Article
Exploring the Druggability of Conserved RNA Regulatory Elements in the SARS‐CoV‐2 GenomeResearch Article Published on 2021-08-032022-10-28 Journal: Angewandte Chemie (International ed. in English) [Category] Coronavirus, SARS, [키워드] assays binding Compound conserved COVID19-NMR defined druggability effective element elements experiments fragment screening functional Genome group in vivo Ligand NMR NMR spectroscopy nuclear nucleotides performed positive Region Regulatory RNA RNA genome RNAs SARS-CoV-2 SARS‐CoV‐2 targets [DOI] 10.1002/anie.202103693 PMC 바로가기 [Article Type] Research Article
NMR Based SARS-CoV-2 Antibody ScreeningNMR 기반 SARS-CoV-2 항체 스크리닝Article Published on 2021-06-022022-09-12 Journal: Journal of the American Chemical Society [Category] MERS, SARS, 진단, [키워드] accelerated ACE2 acute respiratory syndrome angiotensin-converting enzyme 2 antibody approach backbone Cell cell membranes complex coronavirus COVID-19 Efficacy entry receptor Host human SARS-CoV-2 identify Immunity Interaction molecular molecular epitope neutralization NMR NMR spectroscopy RBD RBD-specific mAbs Receptor binding domain recognize SARS-CoV-2 Screening Spike protein subsequent the RBD the SARS-CoV-2 therapy These data to define Vaccine viral infection [DOI] 10.1021/jacs.1c03945 PMC 바로가기 [Article Type] Article
Interactions of SARS-CoV-2 envelope protein with amilorides correlate with antiviral activitySARS-CoV-2 외피 단백질과 아밀로라이드의 상호작용은 항바이러스 활성과 관련이 있습니다Research Article Published on 2021-05-182022-09-10 Journal: PLoS Pathogens [Category] SARS, 치료제, [키워드] activity addition Amiloride amilorides antiviral activity antiviral potency Author binding binding affinity binding site biochemical budding C-terminal causative agent change channel inhibitors characterized chemical shift Complete complex conformation conformations contribute correlated COVID-19 COVID-19 treatment cytoplasmic domain Drug discovery E protein envelope exhibit form foundation full-length SARS-CoV-2 group Guidance Health hexadecylphosphocholine hydrophobic information inhibitor inhibitors Interaction interactions ion channel lack linker membrane protein molecular Mutation N-terminal NMR spectroscopy Novel coronavirus novel coronavirus SARS-CoV-2 pandemic pandemic of COVID-19 Pathogenesis Perturbation Protein Protein conformation pyrazine rapid progress residue residues respiratory respiratory infection responsible SARS-CoV-2 SARS-CoV-2 envelope structural protein structural proteins Structure threat transmembrane transmembrane domain Treatment truncation Vaccines Vero E6 VERO E6 cells Viral viral genome Viral proteins viral replication virion assembly virus-like particles while [DOI] 10.1371/journal.ppat.1009519 PMC 바로가기 [Article Type] Research Article
Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology ApplicationsMolecular Biosciences Published on 2021-05-102022-10-31 Journal: Frontiers in Molecular Biosciences [Category] COVID-19, [키워드] accelerate accessory proteins application approach approach Biology caused cell-free protein synthesis COVID-19 criteria Drug screening effort group Infectious disease Interaction International intrinsically disordered region Laboratory macromolecular majority molecular component NMR NMR spectroscopy nonstructural proteins organized produced Production Protein Proteins protocol provided recombinant Research resource RNA SARS-CoV-2 SARS-CoV-2 protein structural proteins the Betacoronavirus the SARS-CoV-2 valuable resource Viral protein [DOI] 10.3389/fmolb.2021.653148 PMC 바로가기 [Article Type] Molecular Biosciences
Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAsResearch Article Published on 2021-05-042022-10-28 Journal: Angewandte Chemie (International ed. in English) [Category] Coronavirus, SARS, [키워드] 2D NMR spectroscopy analyzed application approach boost CEST correlation demonstrated ENhance experiment explained faster Genome introduced labile limitation Magnetization magnitude mechanism NMR NMR spectroscopy NOESY nucleic acids proton protons recorded resulting RNA RNA fragment RNAs SARS-CoV-2 SARS‐CoV‐2 saturations selected selective sensitivity structures transfer [DOI] 10.1002/anie.202015948 PMC 바로가기 [Article Type] Research Article