Open reading frame – Page 2 – Communicable Diseases Research

Performance of the TaqMan COVID-19 Pooling Kit for detection of SARS-CoV-2 in asymptomatic and symptomatic populations

무증상 및 유증상 집단에서 SARS-CoV-2 검출을 위한 TaqMan COVID-19 풀링 키트의 성능

Article

Published on 2022-06-102022-09-11
Journal: PLoS ONE [Category] COVID19(2023년), SARS, 치료기술, 치료법, 치료제,

Genomic Epidemiology of Imported Cases of COVID-19 in Guangdong Province, China, October 2020 – May 2021

2020년 10월 - 2021년 5월 중국 광둥성에서 수입된 COVID-19 사례의 게놈 역학

Article

Published on 2022-05-202022-09-11
Journal: Biomedical and environmental sciences : BES [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amino acid Analysis analyzed Asian B.1.1.7 B.1.351 Characteristics China Complete coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient COVID-19 patients D614G mutation detect Diversity Europe evaluate evaluated Frame Frequency genome sequence Government Guangdong Guangzhou hazard High-throughput sequencing High-throughput sequencing technology highest homology infected with SARS-CoV-2 Influenza initiative Lineage lineages Missense mutations molecular MOST Mutation Next-generation sequencing North America nucleotide occurred Open reading frame ORF1a ORF7b Orf9b pandemic pangolin Patient peaking Phylogenetic tree proportion Protein reported SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spike protein suggested the spike protein The United States throat swab treat United States variant variants viral sequence viral sequences was used were used
[DOI] 10.3967/bes2022.055 PMC 바로가기 [Article Type] Article

Isothermal gene amplification coupled MALDI-TOF MS for SARS-CoV-2 detection

SARS-CoV-2 검출을 위한 등온 유전자 증폭 결합 MALDI-TOF MS

Article

Published on 2022-05-152022-09-11
Journal: Talanta [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료법, 치료제,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification Analysis coronavirus COVID-19 current demonstrated detect early diagnosis effective false-negative false-negative results Frame Gene detection infected individual infected individuals isothermal lack MALDI-TOF MS MERS MERS-CoV Mutation nucleocapsid Open reading frame other coronaviruses pandemic pseudovirus risk Rolling Rolling circle amplification Saliva SARS-CoV SARS-CoV-2 SARS-CoV-2 detection SARS-like coronavirus severe acute respiratory syndrome Coronavirus specificity temperature Urine Vaccines Virus analysis. water
[DOI] 10.1016/j.talanta.2022.123297 PMC 바로가기 [Article Type] Article

Analysis of clinical characteristics of 66 pediatric patients with B.1.617.2 (Delta) variant of COVID-19

COVID-19의 B.1.617.2(델타) 변이형 소아 환자 66명의 임상적 특성 분석

Article

Published on 2022-05-012022-09-11
Journal: World Journal of Pediatrics [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료법,

[키워드] 24 hour 24 hours accumulate acute respiratory syndrome acute respiratory syndrome coronavirus Admission age All the patient all the patients Analysis analyzed antibody B.1.617.2 B.1.617.2 (Delta) cardiac troponin Cardiac troponin I Characteristics children children with COVID-19 clinical Clinical characteristics clinical experience Clinical features clinical symptom Clinical symptoms Cluster coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus disease 2019 (COVID-19) COVID-19 Cycle threshold value Cycle threshold values D-dimer Delta description disease onset erythrocyte sedimentation erythrocyte sedimentation rate evaluate Frame Gender Genome gradually increased group help hospital IgG IgM IgM antibodies IgM antibody Immunoglobulin Incubation period laboratory data medical staff Mild moderate nucleocapsid protein Open reading frame open reading frame 1 Pediatric patient positive respiratory sedimentation rate severe acute respiratory syndrome Coronavirus statistically significant difference Symptom tested titers troponin I variant
[DOI] 10.1007/s12519-022-00529-1 PMC 바로가기 [Article Type] Article

Profiling of SARS-CoV-2 Subgenomic RNAs in Clinical Specimens

임상 표본에서 SARS-CoV-2 서브게놈 RNA의 프로파일링

Article

Published on 2022-04-272022-09-11
Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제,

[키워드] accessory protein age applied association canonical canonical sgRNA canonical sgRNAs changes in Characteristics clinical Clinical characteristics clinical samples collected COVID-19 COVID-19 patient COVID-19 patients deleted diagnostic discontinuous transcription event expression profile followed by Frame frameshift full-length genomic RNA identify independent Infection Isolation large cohort life cycle majority management marker MONITOR monitoring Next-generation sequencing Numerous Open reading frame ORF10 ORF3a ORF6 ORF7a ORF7b ORF8 Patient PCR positive Potential biomarker potential biomarkers produced profile Profiling progression Protein provide real-time RT-PCR respiratory respiratory sample Respiratory samples RNA RNA-Seq RT-PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 sgRNA SARS-CoV-2 sgRNAs sgRNA sgRNAs specimen specimen collection Stool stool samples subgenomic RNA subgenomic RNAs subgenomic. the patient transcript translation Treatment treatment of COVID-19 patients undetectable variant Viral load
[DOI] 10.1128/spectrum.00182-22 PMC 바로가기 [Article Type] Article

Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8

SARS-CoV-2 ORF8에 의한 인터루킨-17A의 바이러스 모방

Article

Published on 2022-04-262022-09-11
Journal: mBio [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘,

[키워드] activate Activation acute respiratory syndrome acute respiratory syndrome coronavirus binding blood monocyte blood monocytes clinical presentation Clinical presentations Coagulation contribute coronavirus COVID-19 COVID-19 pathogenesis cytokine deleted disease disease pathogenesis dysregulation fibrosis Frame Gene Expression glycoprotein Host IL-17 IL-17A induce infected with SARS-CoV-2 Infection Inflammation Inflammatory response Inflammatory responses interleukin Mild milder infection mimicry molecular molecular mechanism Monocytes myriad Open reading frame ORF8 ORF8 protein outcome outcomes pathway potential therapeutic target Protein receptor reduced reveal robust SARS-CoV-2 SARS-CoV-2 variants secreted severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe inflammation Signaling significantly Transcriptome transcriptome analysis Trigger triggering upregulated variant viral IL-17. with mild disease
[DOI] 10.1128/mbio.00402-22 PMC 바로가기 [Article Type] Article

SARS-CoV2 RT-PCR assays: In vitro comparison of 4 WHO approved protocols on clinical specimens and its implications for real laboratory practice through variant emergence

SARS-CoV2 RT-PCR 분석: 임상 표본에 대한 4가지 WHO 승인 프로토콜의 시험관 내 비교 및 변이체 출현을 통한 실제 실험실 실습에 대한 의미

Article

Published on 2022-03-282022-09-11
Journal: Virology Journal [Category] SARS, 변종,

[키워드] Analysis analyzed approved Berlin protocol CDC Charité China choice COVID-19 demonstrated described Diagnosis evaluate false False negative False positive Genome Hong Kong implication in vitro Internal control Interpretation IPP Laboratory Multiplexing Mutation nasopharyngeal sample Nasopharyngeal samples Nasopharyngeal swab nasopharyngeal swabs New Nsp12 Nsp9 Open reading frame ORF1ab Paris PCR testing positive protocol provided Randomly RdRP recommendation RNA-dependent RNA polymerase RT-PCR RT-PCR testing SARS-CoV2 SARS-CoV2 genome selected sensitivity specimen specimens target targets tested Tunis variant Viral load WHO worsened
[DOI] 10.1186/s12985-022-01784-4 PMC 바로가기 [Article Type] Article

Assessment of SARS-CoV-2 RNA shedding in semen of 36 males with symptomatic, asymptomatic, and convalescent infection during the first and second wave of COVID-19 pandemic in Italy

이탈리아에서 COVID-19 대유행의 1차 및 2차 파동 동안 증상, 무증상 및 회복기 감염이 있는 36명의 남성 정액에서 SARS-CoV-2 RNA 배출 평가

Article

Published on 2022-03-012022-09-11
Journal: Asian journal of andrology [Category] SARS, 치료기술, 치료제,

[키워드] ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus-2 angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 assessment Asymptomatic Chain Reaction Clinical practice convalescent coronavirus coronavirus disease Coronavirus disease-19 Coronavirus-2 COVID-19 COVID-19 pandemic defined detectable detection determine Diagnosis enrolled Evidence evidence of Frame Infection interview Italy male medical history men Mild Nasopharyngeal swab nasopharyngeal swabs nested PCR no evidence of no symptom Open reading frame ORF Patient PCR persistence polymerase chain reaction positive public health real-time reverse transcriptase-polymerase chain reaction real-time RT-PCR receptor recovering from COVID-19 Result reverse transcriptase reverse transcriptase-polymerase chain reaction risk of transmission RT-PCR sampling SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA second wave Semen Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus sexual intercourse sexual transmission symptomatic Symptoms was performed
[DOI] 10.4103/aja2021103 PMC 바로가기 [Article Type] Article

Limited Recognition of Highly Conserved Regions of SARS-CoV-2

SARS-CoV-2의 고도로 보존된 지역에 대한 제한된 인식

Research Article

Published on 2022-02-232022-09-10
Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 치료법,

[키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antigen-specific CD8+ T cells approach CD8 circulating Community Comparative conservation conserved conserved regions convalescent coronavirus coronavirus disease coronavirus strains COVID-19 Critical cross-protective immunity cross-reactive disease encoded protein epitope epitope mapping Epitopes exposure to FIVE Frame generate immune protection immune response immune responses immunodominant implication individuals less likelihood Limited memory memory T cells nucleocapsid observation Open reading frame ORF1 ORF1ab overcome pandemic Participants polyprotein Preexisting immunity protective role Protein Recognition Region required respiratory robust SARS-CoV-2 SARS-CoV-2 infected cells seasonal coronaviruses Sequence analysis severe acute respiratory syndrome Coronavirus severe COVID-19 severe disease T cell T cell response T cell responses T cells T-cell epitope T-cell epitopes T-cell Response T-cell responses understanding unlikely vaccination volunteers
[DOI] 10.1128/spectrum.02780-21 PMC 바로가기 [Article Type] Research Article

Are There Hidden Genes in DNA/RNA Vaccines?

Immunology

Published on 2022-02-082022-11-01
Journal: Frontiers in Immunology [Category] COVID-19,

[키워드] Accessory genes administration antigenic bioinformatics clinical outcomes codon optimization connection contribute death DNA and RNA DNA vaccine Effects examined highlight inactivated information Moderna mRNA-1273 vaccine morbidity Mortality mRNA vaccine offer Open reading frame ORF ORF-Sh ORF2b ORFs overlap overlapping Pain peptides positive predicted produced Protein Proteins reported resources respiratory RNA vaccine SARS-CoV-2 SARS-CoV-2 protein selected sequence shown Spike protein structure-function the spike protein translation Treatment utility vaccination vaccination rate Vaccine vaccine candidate Vaccine design Vaccine development Vaccines virus wild-type
[DOI] 10.3389/fimmu.2022.801915 PMC 바로가기 [Article Type] Immunology