Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system원핵생물 발현 시스템에서 기능적으로 활성인 재조합 SARS-CoV-2(COVID-19) 3C 유사 프로테아제 및 가용성 불활성 3C 유사 프로테아제-RBD 키메라의 생산Article Published on 2022-06-202022-09-11 Journal: Epidemiology and Infection [Category] COVID19(2023년), SARS, 치료제, [키워드] 3C-like protease acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition antigenicity approach blocked carrier cells Chimer chimeric chymotrypsin co-expressed complex coronavirus COVID-19 COVID-19 vaccines cysteine cysteine protease cysteine proteases dimeric disease drug ELISAs enzyme Escherichia coli Escherichia coli BL21 exhibit expression function inactive inhibitors inhibitory main protease non-structural protein non-structural proteins Papain Papain-like protease PLPro polyproteins pp1a pp1ab processing produced Production prokaryotic expression system protease Proteases Protein purification RBD Receptor binding domain RTC SARS-CoV-2 Serine serum severe acute respiratory syndrome Coronavirus solubility Spike protein target tested the Spike Transcription vaccinated individual vaccinated individuals viral replication [DOI] 10.1017/S0950268822001078 PMC 바로가기 [Article Type] Article
Inhibition of SARS-CoV-2 replication by zinc gluconate in combination with hinokitiol히노키티올과 함께 글루콘산아연에 의한 SARS-CoV-2 복제 억제Article Published on 2022-06-012022-09-11 Journal: Journal of inorganic biochemistry [Category] COVID19(2023년), SARS, 치료제, [키워드] 3CLpro acute respiratory syndrome coronavirus antiviral therapy approach binding catalytic site chymotrypsin Combination Concentration coronavirus 2 coronavirus disease Coronavirus disease 2019 Coronavirus infections COVID-19 elevated Enzyme inhibition exhibited experimental evidence global public health gluconate hinokitiol in vitro inhibit inhibited inhibition inhibitory Ionophore mammalian cells metal ion MPro pandemic Papain Papain-like protease PLPro potential benefit potential benefits protease Protease activity Proteases protein structure proteolytic proteolytic activity residues respiratory SARS-CoV-2 SARS-CoV-2 replication screened Severe acute respiratory syndrome severe acute respiratory syndrome coronavirus 2 significantly small molecule target Treatment Zinc zinc gluconate Zinc gluconate. [DOI] 10.1016/j.jinorgbio.2022.111777 PMC 바로가기 [Article Type] Article
Mass Spectrometric Assays Reveal Discrepancies in Inhibition Profiles for the SARS-CoV-2 Papain-Like Protease질량 분석 분석은 SARS-CoV-2 파파인 유사 프로테아제에 대한 억제 프로필의 불일치를 보여줍니다Article Published on 2022-05-042022-09-11 Journal: ChemMedChem [Category] COVID19(2023년), SARS, 치료제, [키워드] applied assays auranofin clinical development cysteine functional highlighting in vitro inhibit inhibition inhibitor inhibitors M pro mass mass spectrometry mechanisms MONITOR MPro Nirmatrelvir non-structural proteins Nucleophilic cysteine protease Papain Papain-like protease PF-07321332 PF-07321332/nirmatrelvir PLPro profile protease protease inhibition Proteases reported SARS-CoV-2 SARS-CoV-2 main protease SARS-CoV-2 main protease/Mpro SARS-CoV-2 papain-like protease/PLpro selected selective targets the SARS-CoV-2 utility viral protease inhibition. viral replication [DOI] 10.1002/cmdc.202200016 PMC 바로가기 [Article Type] Article
Docking Analysis of Some Bioactive Compounds from Traditional Plants against SARS-CoV-2 Target ProteinsSARS-CoV-2 표적 단백질에 대한 전통적인 식물의 일부 생리 활성 화합물의 도킹 분석Article Published on 2022-04-202022-09-11 Journal: Molecules [Category] COVID19(2023년), SARS, 치료제, [키워드] Absorption binding affinity binding energy binding site bioactive compounds caffeic acid Cepharanthine Chimera Compound COVID-19 COVID-19 patients Curcumin cytochrome P450 Cytochrome P450 enzymes demonstrated docked drug-likeness exhibited Express expressed flavin global pandemic inhibited inhibiting inhibitor inhibitors inhibitory effect introduced Ligand ligands Medicine molecular molecular docking Multidrug resistance Papain Papain-like protease pharmacokinetic plant plants PLPro predicted prediction protease Protein protocol Quinine RdRP required Research Result RNA RNA dependent RNA polymerase RNA polymerase rule S-protein SARS-CoV-2 Spike protein suggested target target protein target proteins tested the disease theaflavin these compound these compounds Traditional traditional plants. treating COVID-19 patient Treatment viral spike protein Withaferin A [DOI] 10.3390/molecules27092662 PMC 바로가기 [Article Type] Article
Computational investigation of binding of chloroquinone and hydroxychloroquinone against PLPro of SARS-CoV-2SARS-CoV-2의 PLPro에 대한 클로로퀴논과 하이드록시클로로퀴논의 결합에 대한 전산 조사Article Published on 2022-04-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] Amino acid amino acid residue anticipated approved binding binding affinity carried Chloroquine cleavage complex conformational coronavirus SARS-CoV-2 COVID-19 docked drug target effective Epidemic FDA FDA approved drug HCQ Hydroxychloroquine identify Immune suppression inhibitor inhibitors of SARS-CoV-2 integrity involved MDs mechanism mechanism of action molecular molecular docking molecular dynamics Molecular dynamics simulation molecular dynamics simulations molecular target Mortality MPro novel Novel coronavirus pandemic Papain PLPro polyprotein processing protease repurposing rising SARS-CoV-2 SARS-CoV2 Simulation study the SARS-CoV-2 therapeutic interventions therapeutic targeting [DOI] 10.1080/07391102.2020.1844804 PMC 바로가기 [Article Type] Article
In Silico Screening of Natural Products as Potential Inhibitors of SARS-CoV-2 Using Molecular Docking Simulation분자 도킹 시뮬레이션을 사용한 SARS-CoV-2의 잠재적 억제제로서의 천연물 스크리닝Article Published on 2022-03-012022-09-11 Journal: Chinese journal of integrative medicine [Category] SARS, 치료제, [키워드] 3CL 3CL pro ABL1 acting activated acute respiratory syndrome acute respiratory syndrome coronavirus angiotensin angiotensin converting enzyme anti-SARS-CoV-2 berbamine betulinic acid binding affinity calcineurin carried Cepharanthine chymotrypsin Chymotrypsin-like protease Compound compounds coronavirus derivative drug emodin enzyme glucogallin homolog human coronaviruses in-silico juglanin leukemia molecular molecular docking molecular docking studies Molecular docking study MOST murine natural natural products-derived anti-SARS-CoV-2 candidates NFAT non-structural protein nonstructural proteins nsp10 nsp13 nsp14 Nsp15 Other Papain Papain-like protease performed Potential product protease Protein quercetin RdRP receptor RNA-dependent RNA polymerase S protein SARS-CoV-2 screened Screening Serine serine 2 severe acute respiratory syndrome Coronavirus Simulation spike glycoprotein structural proteins T-cell T-cells tanshinone tanshinone I target target protein target proteins these compound these compounds transmembrane [DOI] 10.1007/s11655-021-3504-5 PMC 바로가기 [Article Type] Article
Exploring naphthyl derivatives as SARS-CoV papain-like protease (PLpro) inhibitors and its implications in COVID-19 drug discoverySARS-CoV 파파인 유사 프로테아제(PLpro) 억제제로서의 나프틸 유도체 및 COVID-19 약물 발견에 미치는 영향 탐색Article Published on 2022-02-012022-09-11 Journal: Molecular diversity [Category] COVID19(2023년), SARS, 치료제, [키워드] 3CLpro acute respiratory syndrome Analysis applied approach biochemical broad spectrum caused connection coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 derivative Drug discovery dynamic Dynamic simulation Environmental safety enzyme event HCoV Health Health Organization help human coronavirus implication Infection inhibiting inhibitor inhibitors Interaction lifecycle molecular molecular docking multitude Naphthyl derivative novel novel corona virus Novel coronavirus novel coronavirus disease Papain Papain-like protease PLPro protease Proteases provided public health Research robust SARS-CoV SARS-CoV PLpro SARS-CoV-2 Severe acute respiratory syndrome starting point target viral replication World Health Organization [DOI] 10.1007/s11030-021-10198-3 PMC 바로가기 [Article Type] Article
Natural biflavones are potent inhibitors against SARS-CoV-2 papain-like protease천연 비플라본은 SARS-CoV-2 파파인 유사 프로테아제에 대한 강력한 억제제입니다Article Published on 2022-01-012022-09-11 Journal: Phytochemistry [Category] SARS, 치료제, [키워드] Antiviral antiviral immunity cleavage compounds COVID-19 COVID-19 treatment deISGylation. demonstrated effective enzyme exhibited immune evasion in viral inhibitor inhibitors of SARS-CoV-2 inhibitory effect involved molecular docking National natural Natural biflavone nine Papain Papain-like protease promotion protease SARS-CoV-2 Significant Traditional Chinese medicine traditional Chinese medicines Treatment Viral immune evasion viral replication viral spread Virtual screening [DOI] 10.1016/j.phytochem.2021.112984 PMC 바로가기 [Article Type] Article
Coronavirus Infection-Associated Cell Death Signaling and Potential Therapeutic Targets코로나바이러스 감염 관련 세포 사멸 신호 및 잠재적 치료 표적Review Published on 2021-12-092022-09-10 Journal: Molecules [Category] SARS, 치료제, [키워드] (RdRp 3CLpro ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 anti-apoptotic Anti-inflammatory antiviral properties Apoptosis assembly autophagy BCL-2 can be used caused celastrol cell death cell death pathways Chloroquine chloroquine/hydroxychloroquine Compound coronavirus CoV infection COVID-19 COVID-19 treatment Critical Curcumin cysteine Cytokines death deaths Effect Efficacy enzyme expression FIVE flavonoids Frame group Helicase host cell Hydroxychloroquine induce Infection Inflammation inhibiting inhibitor Inhibitory effects interactions involved L-cysteine life cycle mechanism mitigate modify molecular target molecules MPro name natural natural compounds necroptosis NF-κB NF-κB signaling NLRP3 inflammasome non-structural protein nonstructural protein 1 nsp1 Nsp12 occurred Open reading frame ORF7a outcomes Papain play roles PLPro Potential progression proliferation promote Propagation protease Protein proteinase pyroptosis RdRP Regulated cell death Replication responsible RNA RNA dependent RNA polymerase RNA polymerase SARS-CoV infection SARS-CoV-2 SARS-CoV-2-infected patients Serine serine 2 severe acute respiratory syndrome Coronavirus Signaling small molecule specific inhibitor spike Spike protein survival target targeted therapy the disease therapeutic targeting TMPRSS2 TNF transmembrane transmembrane protease serine 2 Treatment variety Viral viral entry viral infection viral life cycle virions XIAP yielding [DOI] 10.3390/molecules26247459 PMC 바로가기 [Article Type] Review
Investigating Fungi-Derived Bioactive Molecules as Inhibitor of the SARS Coronavirus Papain Like Protease: Computational Based StudyMedicine Published on 2021-10-212022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] amino acid residues analyses Analysis Antiviral antiviral therapy approach Auto-dock binding affinity candidate carcinogenicity catechin caused Compound compounds Computational approaches concerning COVID-19 COVID-19 pandemic Critical death develop deviation effective effort fluctuation fungi Gromacs growth hydrogen bonds infections inhibitor Interaction investigated less Like molecule outcomes Papain Papain-like protease PLPro protease Protease inhibitor radius of gyration RMSD RMSF SARS Coronavirus SARS-CoV-2 screened selected the binding affinity time-consuming Toxicity WHO World Health Organization [DOI] 10.3389/fmed.2021.752095 PMC 바로가기 [Article Type] Medicine