Human immunoglobulin from transchromosomic bovines hyperimmunized with SARS-CoV-2 spike antigen efficiently neutralizes viral variantsSARS-CoV-2 스파이크 항원으로 과면역된 트랜스염색체 소의 인간 면역글로불린은 바이러스 변이체를 효율적으로 중화합니다Article Published on 2022-04-292022-09-11 Journal: Human vaccines & immunotherapeutics [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus amino-acid anti-SARS-CoV-2 anti-SARS-CoV-2 immunoglobulin bovine convalescent coronavirus COVID-19 Deletion deletions DNA dose E484K effective Effectiveness followed by Human human immunoglobulin Human plasma Immunity immunization Immunoglobulin in vitro inhibit insect cells mAb mAbs monoclonal antibodies monoclonal antibody mutant N501Y neutralization neutralization potency neutralize Plasmid plasmid DNA polyclonal polyclonal antibodies produced purified reduce repeated respiratory resulting retained S gene S protein S variant S477N SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike antigen severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Spike protein stomatitis Substitution substitutions the SARS-CoV-2 therapeutic transchromosomic bovines Vaccines variant variants variants of concern vesicular stomatitis vesicular stomatitis virus viral variant VSV Wuhan strain [DOI] 10.1080/21645515.2021.1940652 PMC 바로가기 [Article Type] Article
Phosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics유행성 진단을 위한 규조류 Phaeodactylum tricornutum에서 SARS-CoV-2 수용체 결합 도메인의 인산염 조절 발현Article Published on 2022-04-292022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] alleviate anti-RBD antibiotic Antigen approach Betacoronavirus binding Biomedical research C-terminal can be used caused Cell coronavirus COVID-19 pandemic cross-reactive detect develop diagnostics domain Donor eukaryotic expressed expression expression plasmids finding glycosylated growth media histidine human ACE2 receptor inhibited iron lateral flow mammalian cell overexpression pandemic performed phosphate Plasmid polyclonal antibodies promoter purified RBD Receptor-binding domain retained SARS-CoV-2 SARS-CoV-2 proteins SARS-CoV-2 spike protein sensitivity serum specific IgG antibodies specific IgG antibody Spike protein Synthetic biology TEV protease the RBD the receptor-binding domain the SARS-CoV-2 Therapeutics Treatment type strain Viral antigen wild-type strain yield [DOI] 10.1038/s41598-022-11053-7 PMC 바로가기 [Article Type] Article
Detection of VAMP Proteolysis by Tetanus and Botulinum Neurotoxin Type B In Vivo with a Cleavage-Specific Antibody테타누스 및 보툴리눔 신경독소 B형에 의한 VAMP 단백질 분해의 생체 내 검출 및 절단 특이 항체 사용Article Published on 2022-04-142024-08-19 Journal: International Journal of Molecular Sciences [Category] 파상풍, [키워드] botulinum neurotoxins polyclonal antibodies SNARE proteins tetanus neurotoxins vesicle-associated membrane protein VAMP.
Evaluating Antibody Mediated Protection against Alpha, Beta, and Delta SARS-CoV-2 Variants of Concern in K18-hACE2 Transgenic MiceK18-hACE2 형질전환 마우스에서 우려되는 알파, 베타 및 델타 SARS-CoV-2 변이체에 대한 항체 매개 보호 평가Article Published on 2022-03-232022-09-11 Journal: Journal of Virology [Category] MERS, SARS, 변종, 진단, 치료법, [키워드] Alpha Alpha VOC angiotensin angiotensin converting enzyme Beta Brain can be used concern convalescent convalescent plasma COVID-19 COVID-19 pandemic COVID-19 patient Critically ill decrease Delta Delta VOC disease dose Efficacy elevated enzyme evaluate Evaluating hACE2 hcp Human IgG titer immune evasion immunization in vivo model K18 K18-hACE2 K18-hACE2 transgenic mice lethality lung Mediated mice modeling modeling COVID-19 monoclonal antibody Mortality not decreased nucleocapsid nucleocapsid IgG pandemic passive immunity passive immunization polyclonal antibodies polyclonal antibody problem protection RBD Receptor binding domain response SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variants serum single dose Strains survival These data transgenic Transgenic mouse Treatment treatment group Vaccine variants variants of concern Viral Viral RNA while [DOI] 10.1128/jvi.02184-21 PMC 바로가기 [Article Type] Article
Multiple SARS-CoV-2 Variants Exhibit Variable Target Cell Infectivity and Ability to Evade Antibody Neutralization다양한 SARS-CoV-2 변이체가 다양한 표적 세포 감염성과 항체 중화 회피 능력을 나타냄Article Published on 2022-03-162022-09-11 Journal: Frontiers in Immunology [Category] MERS, SARS, 변종, 진단, [키워드] Ability addition antigenicity B.1.617.2 BBIBP-CorV BBIBP-CorV vaccine binding affinity blocked changed CoronaVac CoronaVac vaccine coronavirus disease Coronavirus disease 2019 COVID-19 D614G elicited Evolution of SARS-CoV-2 exacerbated host cell host cells Immune escape immune evasion in viral infect Infection Infectivity inhibit inhibitors less mAb mAbs monoclonal antibody Multiple Mutation Neutralizing Neutralizing antibodies neutralizing antibody pandemic polyclonal antibodies polyclonal antibody Protease inhibitor protease inhibitors Protein proteolytic provide pseudovirus receptor S protein SARS-coronavirus SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sera significantly stomatitis susceptible cell target the S protein therapeutic vaccination variable variant variants variants of concern vesicular stomatitis vesicular stomatitis virus Viral viral entry viral infection viral infectivity virus infection VoC was used were assessed [DOI] 10.3389/fimmu.2022.836232 PMC 바로가기 [Article Type] Article
Development of rapid antigen test prototype for detection of SARS-CoV-2 in saliva samples타액 샘플에서 SARS-CoV-2 검출을 위한 신속 항원 검사 프로토타입 개발Original Article Published on 2022-02-252022-09-10 Journal: Upsala Journal of Medical Sciences [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 anti-RBD anti-receptor-binding domain Anti-spike antibody Antigen antigen test Asymptomatic capture Chain Reaction clinical analysis Cohort Combination combinations coronavirus coronavirus disease COVID-19 demonstrated detect detection antibody development diagnostic Diagnostic accuracy Diagnostic method diagnostic methods domain early stage ELISA ENhance enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay evaluated exhibited followed by hospitalized COVID-19 patient hospitalized COVID-19 patients immunized improvement individual Infection interquartile range lateral flow lateral flow assay median time NPV onset of symptom pilot study point-of-care testing polyclonal antibodies polyclonal antibody polymerase chain reaction positive PPV RBD reference method respiratory Result reverse transcription Reverse transcription-polymerase chain reaction rRBD rRT-PCR Saliva saliva samples sample collection SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV-2 spike protein SARS-CoV-2 virus sensitivity severe acute respiratory syndrome Coronavirus specificity Spike protein stratified symptomatic patient tested the median the SARS-CoV-2 validation study virus was used were used [DOI] 10.48101/ujms.v127.8207 PMC 바로가기 [Article Type] Original Article
A SARS-CoV-2 variant elicits an antibody response with a shifted immunodominance hierarchySARS-CoV-2 변이체는 면역 우세 계층이 이동된 항체 반응을 유발합니다Research Article Published on 2022-02-082022-09-10 Journal: PLoS Pathogens [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] (Beta 501Y.V2 affected antibodies antibody Antibody Response B.1.351 B.1.351 variant Beta conserved delta variant determine Donor donors elicit elicited epitope Epitopes Frequency Human humans Immunity immunodominance immunodominant infected with SARS-CoV-2 Infection Influenza isolate isolates less mutant mutated Mutation neutralization Neutralizing activity polyclonal antibodies polyclonal antibody produced RBD Region residue SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sera serum Specificities specificity spike epitopes spike receptor-binding domain susceptibility the antibody response the RBD vaccination variant variants Viral viral isolates viral spike virus viruses while [DOI] 10.1371/journal.ppat.1010248 PMC 바로가기 [Article Type] Research Article
Efficacy of High-Dose Polyclonal Intravenous Immunoglobulin in COVID-19: A Systematic ReviewSystematic Review Published on 2022-01-092022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] 95% CI 95% confidence interval Analysis approach binary outcomes Care carried certainty of evidence CIs Cochrane Library Control Controlled trial coronavirus disease COVID-19 COVID-19 patient COVID-19 patients death Efficacy and safety evaluate Evidence Hospital stay immunosuppressants incidence Intravenous immunoglobulin Intravenous immunoglobulins investigated mean difference measure Meta-analysis methodology Moderate COVID-19 Mortality non-randomized outcome outcome measure participant Patient patients polyclonal polyclonal antibodies Randomized controlled trial RCT recipient reduced reduced risk resolved review risk SARS-CoV-2 Serious Adverse Event significant difference significantly subject Support systematic literature search systematic review therapeutic strategy therapy Treatment was performed [DOI] 10.3390/vaccines10010094 PMC 바로가기 [Article Type] Systematic Review
Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology: Implementation and Clinical Validation신속하고 정량적이며 매우 민감한 Covid-19 혈청학을위한 라벨이없는 플라즈몬 바이오 센서 : 구현 및 임상 검증Article Published on 2021-12-312022-09-10 Journal: Analytical Chemistry [Category] Fulltext, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification anti-SARS-CoV-2 antibody biosensor clinical clinical samples coronavirus COVID-19 COVID-19 pandemic Detection limit diagnostic diagnostic sensitivity diagnostics epidemiological Immunity Immunoglobulin immunological Laboratory management N protein negative sample offer peptide polyclonal antibodies positive quantification Rapid RBD respiratory SARS-CoV-2 sensitive sensitivity serological serological test Serological tests serology severe acute respiratory syndrome Coronavirus specificity Surveillance the disease Treatment vaccination validation with COVID-19 [DOI] 10.1021/acs.analchem.1c03850 PMC 바로가기 [Article Type] Article
Waning antibodies from inactivated SARS-CoV-2 vaccination offer protection against infection without antibody-enhanced immunopathology in rhesus macaque pneumonia models비활성화된 SARS-CoV-2 백신 접종으로 인한 항체 약화는 붉은털 원숭이 폐렴 모델에서 항체 강화 면역병리학 없이 감염에 대한 보호를 제공합니다Letter Published on 2021-11-212022-09-10 Journal: Emerging Microbes & Infections [Category] Coronavirus, SARS, 진단, 치료기술, [키워드] absence acute phase acute respiratory syndrome acute respiratory syndrome coronavirus ADE alveolar anti-SARS-CoV-2 anti-SARS-CoV-2 antibody anti-SARS-CoV-2 IgG antibody Antibody Response Antibody-dependent enhancement bronchioles Bronchoalveolar lavage Bronchoalveolar lavage fluid Cell cellular immunity clinical trial control group coronavirus Coronaviruses elevated epithelial Evidence evidence of humans IL-33 IL-8 immunology immunopathology inactivated Inactivated Vaccines Infection initial MCP-1 MERS-CoV Middle East Middle East respiratory syndrome Coronavirus Mild mild to moderate moderate Mucus murine neutralizing antibody NHP no evidence of observation offer passive transfer Pneumonia polyclonal antibodies polyclonal antibody postvaccination preclinical studies preclinical study pulmonary immunopathology reported respiratory Respiratory disease respiratory diseases respiratory syndrome coronavirus rhesus SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 vaccination SARS-CoV-2 vaccine SARS-CoV-2 vaccines severe acute respiratory syndrome Coronavirus subneutralizing antibodies supported transfer Typical vaccination Vaccine virus virus infection [DOI] 10.1080/22221751.2021.2002670 PMC 바로가기 [Article Type] Letter