Emergency SARS-CoV-2 variants of concern: rapidly direct RT-qPCR detection without RNA extraction, clinical comparison, cost-effective, and high-throughput우려되는 긴급 SARS-CoV-2 변종: RNA 추출 없이 신속하게 직접 RT-qPCR 검출, 임상 비교, 비용 효율적 및 높은 처리량Article Published on 2022-06-022022-09-11 Journal: Aging (Albany NY) [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 alternative method analytical sensitivity Chain Reaction characterized complex coronavirus coronavirus disease Coronavirus disease 2019 cost-effective diagnosis COVID-19 Diagnosis direct RT-qPCR dominant E gene Emergency Evolution faster gold gold standard high-throughput. labor limit of detection mutations nasopharyngeal swabs Negative predictive value pandemic polymerase chain polymerase chain reaction positive Predictive value protocol real-time reverse transcriptase Real-time reverse transcriptase polymerase chain reaction reverse transcriptase Reverse transcriptase polymerase chain reaction RNA extraction RT-PCR RT-qPCR SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 VoC sensitivity severe acute respiratory syndrome Coronavirus significantly Spike protein thermocyclers variant variants variants of concern [DOI] 10.18632/aging.204095 PMC 바로가기 [Article Type] Article
Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing현장 진료 테스트에서 SARS-CoV-2 바이러스의 신속한 검출을 위한 실시간 역전사효소 루프 매개 등온 증폭에서 이월 오염 제거Article Published on 2022-04-202022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] COVID19(2023년), SARS, 치료기술, [키워드] Accuracy amplification assays carryover contamination COD Cod uracil DNA glycosylase (Cod-UNG) contaminant Contamination COVID-19 detection diagnosis of SARS-CoV-2 diagnostic Diseases DNA eliminated Elimination feasibility glycosylase Infectious diseases isothermal amplification LAMP Loop-mediated isothermal amplification Loop-mediated isothermal amplification (LAMP) on-site testing Pathogens POC point-of-care point-of-care. Prevent problem Rapid reaction real-time reverse transcriptase reverse transcriptase robust rRT-PCR SARS-CoV-2 SARS-CoV-2 virus sensitivity specificity Testing the SARS-CoV-2 the SARS-CoV-2 virus Transmission uracil [DOI] 10.3389/fcimb.2022.856553 PMC 바로가기 [Article Type] Article
Swab test in biological fluids as predictor of COVID-19 transmission risk during surgery: a prospective cross-sectional study from an Italian COVID center수술 중 COVID-19 전염 위험을 예측하는 생물학적 유체의 면봉 검사: 이탈리아 COVID 센터의 전향적 횡단면 연구Article Published on 2022-03-302022-09-11 Journal: BMC Surgery [Category] SARS, 치료기술, [키워드] acute respiratory syndrome biological fluid biological fluids body fluid body fluids Chain Reaction Chest concerning Contagion Contamination coronavirus 2 coronavirus disease Coronavirus disease 2019 COVID COVID-19 COVID-19 pandemic COVID-19 transmission cross-sectional cross-sectional study debate Emergency surgery evaluate Evidence evidence of First wave fluids Italian Laparoscopic surgery nucleic acid Operating rooms Pandemics PCR performed polymerase chain polymerase chain reaction qualitative detection real-time reverse transcriptase respiratory reverse transcriptase risk SARS-CoV-2 SARS-CoV-2 RNA detection SARS-CoV-2 transmission severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 single center surgery surgery. Swab Swab test Thoracic Thoracic Surgery Viral RNA Viral transmission virus would increase [DOI] 10.1186/s12893-022-01571-6 PMC 바로가기 [Article Type] Article
Epidemiological and Clinical Comparative Study for COVID-19 Patients in Babylon Province, Iraq이라크 바빌론 주 코로나19 환자에 대한 역학 및 임상 비교 연구Article Published on 2022-02-282022-09-11 Journal: Archives of Razi Institute [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus age caused Chain Reaction chart China city clinical Clinical diagnosis Clinical signs clinical symptom Clinical symptoms Comparative coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Diagnosis Epidemiology Female patients high temperature hospital indication individual intensity Iraq Laboratory male Nasopharyngeal swab nasopharyngeal swabs outbreak Patient pneumonia. polymerase chain polymerase chain reaction provided public health ranged real-time reverse transcriptase Real-time reverse transcriptase polymerase chain reaction Respiratory droplets Respiratory illness Retrospective study reverse transcriptase SARS-CoV-2 SARS-CoV-2 detection severe acute respiratory syndrome Coronavirus Shortness of breath shown sore throat specimen spread to Swab Symptom Symptoms temperature transmitted virus [DOI] 10.22092/ARI.2021.356550.1869 PMC 바로가기 [Article Type] Article
Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance긴급 SARS-CoV-2 우려 변종: 신속한 탐지 및 감시를 위한 새로운 멀티플렉스 실시간 RT-PCR 분석Research Article Published on 2022-02-232022-09-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 변종, 치료기술, 치료제, [키워드] ΔHV 69/70 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Alpha analysis applied assays B.1.1.7 B.1.351 B.1.617.2 Beta caused circulating clinical sample clinical samples coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 pandemic COVID-19 patient Critical cross-reactivity Delta detect detection diagnostic difficulty E484K E484R effective Efficiency Emergency ENhance Epidemic epidemiological epidemiological surveillance Evolution feasibility FIVE Gamma gold gold standard Government infection control issue K417N K417T L452R Laboratory lineages Local local government multiplex Multiplex PCR Mutation mutations N501Y novel omicron outbreak P.1 P681H P681R PCR PCR assay Previous studies previous study primer probe probes provided public health measures Rapid real-time reverse transcriptase real-time RT-PCR receptor Receptor-binding domain reduced reported respiratory reverse transcriptase RT-PCR S gene SAR-CoV-2 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants Sequencing severe acute respiratory syndrome Coronavirus specificity Spike protein Spread Surveillance susceptibility Taiwan targets the receptor-binding domain the spike protein time intervals Transmissibility treatment regimens understanding upper respiratory Vaccine Vaccines variant variants variants of concern variants of SARS-CoV-2 viruses VoC VOC genotyping VOCs whole-genome sequencing [DOI] 10.1128/spectrum.02513-21 PMC 바로가기 [Article Type] Research Article
Centrifugal Microfluidic Method for Enrichment and Enzymatic Extraction of Severe Acute Respiratory Syndrome Coronavirus 2 RNA중증 급성 호흡기 증후군 코로나바이러스 2 RNA의 농축 및 효소 추출을 위한 원심 미세 유체 방법Article Published on 2022-02-222022-09-11 Journal: Analytical Chemistry [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification analyst analyzed applicability buffer capsid Centrifugal Chain Reaction comparable complex controls coronavirus coronavirus 2 demonstrated development Diagnosis diagnostics downstream effective ENhance enrichment exposure to extraction imperative in viral inhibit Intervention Isolation lysed nasopharyngeal Nasopharyngeal swab nucleic acid nucleic acids Particle pathogen detection pathogenic Pathogens platform polymerase chain polymerase chain reaction positive purification real-time reverse transcriptase real-time RT-PCR removal respiratory reverse transcriptase Reverse transcriptase polymerase chain reaction RNA RNA extraction robust RT-PCR Saliva Sample SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA SARS-CoV-2 virion severe acute respiratory syndrome Coronavirus Surveillance Transmission Transport media Treatment tube Viral RNA viral transport media virus [DOI] 10.1021/acs.analchem.1c05215 PMC 바로가기 [Article Type] Article
Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of ConcernB.1.1.7 우려 변이체와 관련된 SARS-CoV-2의 스파이크 단백질에서 N501Y 돌연변이 검출을 위한 실시간 RT-PCR 대립형질 판별 분석Research Article Published on 2022-02-162022-09-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 allelic Amino acid B.1.1.7 B.1.351 B.1.617.2 caused characterized circulating concern coronavirus coronavirus disease Coronavirus disease 2019 costly COVID-19 Critical current decrease demonstrated described detection discrimination displaying effort example focus help identify Intensive labor Lineage mutant Mutation N501Y N501Y mutation negative nucleotide P.1 pandemic PCR assay polymorphism positive public health real-time reverse transcriptase real-time RT-PCR respiratory reverse transcriptase reverse transcription PCR RT-PCR RT-PCR assay Sample Sanger sequencing SARS-CoV-2 SARS-CoV-2 lineage sequenced Sequencing several variant several variants severe acute respiratory syndrome Coronavirus single nucleotide Single nucleotide polymorphism SNP specimen spike gene Spread Surveillance tested the Spike turnaround times variants of concern virus VoC VOCs whole-genome whole-genome sequencing wild type [DOI] 10.1128/spectrum.00681-21 PMC 바로가기 [Article Type] Research Article
SARS-CoV-2 is not found in the sweat of COVID-19 positive patientsSARS-CoV-2는 COVID-19 양성 환자의 땀에서 발견되지 않습니다Article Published on 2022-02-012022-09-11 Journal: Irish Journal of Medical Science [Category] COVID19(2023년), SARS, 치료기술, [키워드] alcohol Care Chain Reaction conducted COVID-19 COVID-19 positive patient forehead hospital Human Laboratory pandemic Patient polymerase chain polymerase chain reaction Precaution Prospective Study real-time reverse transcriptase reverse transcriptase Reverse transcriptase polymerase chain reaction RT-PCR SARS-CoV-2 SARS-CoV-2 virus skin Swab swabs Sweat sweating the SARS-CoV-2 virus transmission of SARS-CoV-2 transmission route Transmission. transport medium transported Viral transport medium virus was done [DOI] 10.1007/s11845-021-02537-y PMC 바로가기 [Article Type] Article
The application of a deep learning system developed to reduce the time for RT-PCR in COVID-19 detectionCOVID-19 탐지에서 RT-PCR 시간을 줄이기 위해 개발 된 딥 러닝 시스템의 적용Article Published on 2022-01-242022-09-01 Journal: Scientific Reports [Category] SARS, 진단, 치료기술, [키워드] alternative method an area AUROC Biotechnology Chain Reaction characteristic Computational biology and bioinformatics COVID-19 Critically ill critically ill patients dataset deep learning diagnose diagnostic Health care help IMPROVE Medical research memory PCR tests polymerase chain polymerase chain reaction real-time reverse transcriptase Real-time reverse transcriptase polymerase chain reaction receiver operating characteristic reduce resource reverse transcriptase Reverse transcriptase polymerase chain reaction RT-PCR RT-PCR diagnosis RT-PCR tests sensitivity Short-Term Memory specificity speed tested [DOI] 10.1038/s41598-022-05069-2 PMC 바로가기 [Article Type] Article
Performance Characteristics of the Abbott BinaxNOW SARS-CoV-2 Antigen Test in Comparison to Real-Time Reverse Transcriptase PCR and Viral Culture in Community Testing Sites during November 20202020년 11월 커뮤니티 테스트 사이트에서 Real-Time Reverse Transcriptase PCR 및 바이러스 배양과 비교한 Abbott BinaxNOW SARS-CoV-2 항원 테스트의 성능 특성Article Published on 2022-01-192022-09-11 Journal: Journal of Clinical Microbiology [Category] SARS, 치료기술, [키워드] Abbott Abbott BinaxNOW SARS-CoV-2 Ag Card Administered analyzed Antigen antigen test antigen tests Cell culture collected Community COVID-19 demographic variable Demographic variables disease highest infected individuals less limitation nucleic acid nucleic acid amplification nucleic acid amplification tests participant PCR performance Pima positive Positive predictive value Predictive value questionnaire real-time reverse transcriptase Result reverse transcriptase rRT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 test sensitivity Site specimens swab specimen Symptom symptom onset symptomatic symptomatic individual symptomatic individuals Symptoms Test Testing upper respiratory viral culture viral culture. virus [DOI] 10.1128/JCM.01742-21 PMC 바로가기 [Article Type] Article