Single-cell sorting based on secreted products for functionally defined cell therapies Reviews Published on 2022-07-222022-10-05 Journal: Microsystems & Nanoengineering [Category] 바이오마커, [키워드] accumulating addition Analysis approach approaches autoimmune disorder Cell Cell therapy cellular cellular therapy Critical defined disease Efficacy exacerbated fibrosis functional Genetic Hematological malignancies in vivo individual cell killing labeling lack Migration molecular neurodegenerative disorder patients proliferation remained reporter robust secreted secretion Single Cell single-cell solid tumor therapeutic therapeutic potential Therapies therapy triggering unmet need while [DOI] 10.1038/s41378-022-00422-x [Article Type] Reviews
Using human iPSC-derived kidney organoids to decipher SARS-CoV-2 pathology on single cell level단일 세포 수준에서 SARS-CoV-2 병리를 해독하기 위해 인간 iPSC 유래 신장 오르가노이드 사용Article Published on 2022-07-192022-09-12 Journal: STAR protocols [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] Cell Cell Biology cells changes in Complete kidney kidney organoid kidney organoids Microbiology organ Organoid organoids pathology protocol reveal SARS-CoV-2 Sequencing analysis Single Cell single cell level single cell suspensions Single-cell RNA sequencing STEM stem cells Transwell virus virus infection [DOI] 10.1016/j.xpro.2022.101612 PMC 바로가기 [Article Type] Article
Can spike fragments of SARS-CoV-2 induce genomic instability and DNA damage in the guppy, Poecilia reticulate? An unexpected effect of the COVID-19 pandemicSARS-CoV-2의 스파이크 조각이 구피 포에실리아 망상체의 게놈 불안정성과 DNA 손상을 유발할 수 있습니까? COVID-19 전염병의 예상치 못한 영향Article Published on 2022-06-152022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, [키워드] animals Brain Can catalase correlated COVID-19 COVID-19 pandemic demonstrated dismutase dispersal DNA damage ecosystem Effect ENA evaluate Frequency Freshwater fish gel electrophoresis genomic genotoxic genotoxicity Impact increase in induce knowledge lipid peroxidation liver malondialdehyde mutagenic Mutagenicity New coronavirus nuclear Olive Organisms parameter peptide peptides Protein raised reduction reported SARS-CoV-2 SARS-CoV-2 particle SARS-CoV-2 particles significantly Single Cell Spike protein Superoxide Superoxide Dismutase tail the Spike Toxicity Viral particles water Water pollution [DOI] 10.1016/j.scitotenv.2022.153988 PMC 바로가기 [Article Type] Article
Proper Selection of In Vitro Cell Model Affects the Characterization of the Neutralizing Antibody Response against SARS-CoV-2체외 세포 모델의 적절한 선택은 SARS-CoV-2에 대한 중화 항체 반응의 특성화에 영향을 미칩니다Article Published on 2022-06-072022-09-11 Journal: Viruses [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] antibody Antibody Response BNT162b2 BNT162b2 mRNA BNT162b2 mRNA vaccine Calu Calu-3 cell line cell lines cellular characterization characterized choice collected correlation COVID-19 COVID-19 vaccine Cytopathic effect cytopathic effects demonstrated Entry mechanism entry mechanisms evaluated in vitro increasingly inhibitory activity lack mechanisms Model mRNA mRNA vaccine natural infection Neutralizing Neutralizing activity occur of BNT162b2 PCR pseudovirus pseudoviruses response SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variants SARS-CoV-2 viral Selection sera Single Cell subject Surrogates TMPRSS2 vaccine-induced antibodies vaccine-induced antibody variants Vero Vero E6 Viral viral entry viral entry pathway virus particles VOCs [DOI] 10.3390/v14061232 PMC 바로가기 [Article Type] Article
Ursodeoxycholic acid ameliorates cell migration retarded by the SARS-CoV-2 spike protein in BEAS-2B human bronchial epithelial cellsUrsodeoxycholic acid는 BEAS-2B 인간 기관지 상피 세포에서 SARS-CoV-2 스파이크 단백질에 의해 지연된 세포 이동을 개선합니다Article Published on 2022-06-012022-09-11 Journal: Biomedicine & pharmacotherapy = Biomédecine & phar [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] ACE2 ACE2 expression airway analyzed angiotensin angiotensin converting enzyme angiotensin converting enzyme 2 basal layer BEAS-2B Beas-2B bronchial epithelial cell BEAS-2B cell Beas-2B cells binding bronchial epithelial cell caused Cell Cell migration cell proliferation cells changes in conditions contribute control cells coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 damage damaged differentiation DNA ENhance enzyme epithelial epithelial cell flow cytometry followed by Gene Expression greater HEK293T Hela immunostaining inhibitory Interaction investigated mechanism Migration overexpression Plasmid Prevent Protective RBD receptor Receptor-binding domain reduced repair repair mechanism Repair mechanisms restoration restored RT-PCR SARS- CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein significantly Single Cell SP binding Spike protein syndrome the receptor-binding domain the SARS-CoV-2 the spike protein transfection Treatment Ursodeoxycholic acid Ursodeoxycholic acid (UDCA). virus infection was increased was measured western blotting wound healing [DOI] 10.1016/j.biopha.2022.113021 PMC 바로가기 [Article Type] Article
Rapid and Multiplexed Detection of Single Cells of Salmonella, Escherichia coli O157, and Shigella flexneri in Ground Beef by Flow Cytometryarticle Published on 2022-04-302024-09-01 Journal: Foodborne Pathogens and Disease [Category] 세균성이질, [키워드] Escherichia coli O157 flow cytometry Multiplexed detection Salmonella Shigella flexneri Single Cell [Article Type] article
Analyzing single cell transcriptome data from severe COVID-19 patients중증 코로나19 환자의 단세포 전사체 데이터 분석Article Published on 2022-04-212022-09-11 Journal: STAR protocols [Category] MERS, 바이오마커, 유전자 메커니즘, 진단, [키워드] bioinformatics Cell cell type cell types cellular Complete COVID-19 COVID-19 severity database detect disease-associated enrichment analysis Gene Expression Genomics Health Sciences identify immunology in silico in vitro in vivo marker gene Molecular biology protocol Regulatory resources RNAseq severe COVID-19 severe COVID-19 patient Single Cell single-cell Transcriptome transcriptomics upregulated upregulation [DOI] 10.1016/j.xpro.2022.101379 PMC 바로가기 [Article Type] Article
Normalizing and denoising protein expression data from droplet-based single cell profiling Research Published on 2022-04-192022-10-05 Journal: Nature Communications [Category] 변종, [키워드] protein expression Single Cell [DOI] 10.1038/s41467-022-29356-8 [Article Type] Research
Clinical and translational values of spatial transcriptomics Reviews Published on 2022-04-012022-10-05 Journal: Signal Transduction and Targeted Therapy [Category] 바이오마커, [키워드] alterations Analysis bioinformatics Biomarkers cellular interaction clinical Clinical practice Combination disadvantage evaluate Gene Expression highlight human disease human tissue Inflammation intercellular Interpretation knowledge molecular oncology Pathogenesis protocol RNA sequencing scRNA-seq Sequencing Single Cell spatiotemporal transcriptomics translate translational [DOI] 10.1038/s41392-022-00960-w [Article Type] Reviews
Multiple early factors anticipate post-acute COVID-19 sequelae여러 초기 요인으로 인해 급성 COVID-19 후유증이 예상됩니다.Article Published on 2022-03-032022-09-11 Journal: Cell [Category] MERS, SARS, 바이오마커, 유전자 메커니즘, 진단, 치료기술, [키워드] antibodies association Auto-antibodies CD8 chronic condition chronic conditions Clinical data Computational biology convalescence convalescent COVID-19 COVID-19 diagnosis COVID-19 patient detectability diabetes Diagnosis disease early disease Epstein-Barr virus exhibited exhibiting Factor immune immune states immune system Immunity immunological immunology initial Long COVID metabolomics multi Multi-omics Multiple PASC Patient proteomics resolved risk factor RNAemia SARS-CoV-2 severity Single Cell single-cell single-cell CITE-seq single-cell RNA-seq single-cell secretome single-cell TCR-seq Symptoms T cell T cells Transcriptome Treatment strategies type 2 diabetes unique Viremia virus [DOI] 10.1016/j.cell.2022.01.014 PMC 바로가기 [Article Type] Article