Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoproteinSARS-CoV-2의 전사체 및 프로테옴의 특성화는 스파이크 당단백질에서 푸린 유사 절단 부위의 세포 통과 유도된 프레임 내 결실을 보여줍니다Research Published on 2020-07-282022-08-13 Journal: Genome Medicine [Category] SARS, 유전자 메커니즘, [키워드] 24 nt in-frame deletion analysed Analysis animal challenge model animal challenge models Cell cleavage clinical samples contribute Coronaviruses deleted detection determine Direct RNA sequencing exit expected Frame functional furin furin cleavage site furin site furin-like cleavage site genomic glycoprotein growth Health impacted in-frame deletion infected cells mass spectrometry morbidity morbidity and mortality mutate Novel coronavirus ORF-centric pipeline Pathogenesis peptide peptides phosphopeptide phosphoproteome predicted Protein proteome proteomic Region Research respiratory respiratory pathogen Result reveal S glycoprotein SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 protein SARS-CoV-2 proteins significantly spike glycoprotein subgenomic mRNA subgenomic mRNAs subunit tandem Tandem mass spectrometry the SARS-CoV-2 genome Transcription Transcriptome transcriptomic unique Vaccine variant Variation Vero E6 Vero E6 cell VERO E6 cells viable deletion Viral viral genome sequence viral peptide viral peptides viral stock viral stocks viral transcript viral transcriptome viral transcripts virulence virus virus entry virus-infected cells zoonosis [DOI] 10.1186/s13073-020-00763-0 PMC 바로가기 [Article Type] Research
Genome structure and transcriptional regulation of human coronavirus NL63Research Published on 2004-11-172022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] 2003 Analysis Cell culture coding codon usage compared Coronaviral coronavirus detect experimental analysis expression level GC content Genome genome structure HCoV-229E HCoV-NL63 HCoV-OC43 human coronavirus humans increase indicate indicated infect Inspection leader sequence mRNA mRNAs N proteins NL63 Northern blot analysis nucleocapsid nucleotide ORF3 other coronaviruses Pathogenesis pathogenicity performed powerful tool reflected Replication Result RNA genome SARS Coronavirus SARS-CoV sequenced significantly subgenomic mRNA subgenomic mRNAs Transcription transcriptional regulation viral evolution virus [DOI] 10.1186/1743-422X-1-7 PMC 바로가기 [Article Type] Research