Effect of not vortexing nasopharyngeal and throat swabs on SARS-CoV-2 nucleic acid detection: A pilot studySARS-CoV-2 핵산 검출에 대한 비인두 및 인후 면봉 검사의 효과: 파일럿 연구Article Published on 2022-03-012022-09-11 Journal: Journal of virological methods [Category] SARS, 치료기술, [키워드] Analysis appear assays automated cellular cellular material Ct values cycle threshold Effect eight IMPROVE Laboratory nasopharyngeal nucleic acid nucleic acid amplification nucleic acid tests PCR pilot study positive processing Qualitative reduction in respiratory virus Sample Sample processing SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 nucleic acid SARS-CoV-2 RNA SARS-CoV-2 testing semi-quantitative sensitivity submitted Support Swab swab sample swab samples swabs tested throat swab transport medium Viral transport medium Vortex Vortex. Vortexing VTM while [DOI] 10.1016/j.jviromet.2022.114468 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 Virus Amplification Using a Crumpled Graphene Field-Effect Transistor Biosensor구겨진 그래핀 전계 효과 트랜지스터 바이오센서를 사용한 SARS-CoV-2 바이러스 증폭 검출Article Published on 2021-12-242022-09-11 Journal: ACS Sensors [Category] SARS, 치료기술, [키워드] amplification biosensor cause of death caused Cell clinical sample clinical samples COVID-19 COVID-19 pandemic Critical crumpled graphene FET biosensor death demonstrated detect detection develop diagnose diagnostic diagnostic testing disease DNA double-stranded Double-stranded DNA electrical flat flat graphene FET biosensor. gold gold standard Graphene IMPROVE isothermal amplification LAMP Loop-mediated isothermal amplification maintain NPV point-of-care positive PPV public health reducing reverse transcriptase RT-LAMP SARS-CoV-2 SARS-CoV-2 virus sensitivity single-stranded DNA specificity Spread the SARS-CoV-2 virus the United State transcriptase transistor transport medium United States Viral transport medium VTM VTM clinical samples [DOI] 10.1021/acssensors.1c01937 PMC 바로가기 [Article Type] Article
A Cold Chain-Independent Specimen Collection and Transport Medium Improves Diagnostic Sensitivity and Minimizes Biosafety Challenges of COVID-19 Molecular Diagnosis콜드 체인 독립적인 표본 수집 및 운송 매체는 진단 감도를 개선하고 COVID-19 분자 진단의 생물학적 안전성 문제를 최소화합니다Research Article Published on 2021-12-082022-09-10 Journal: Microbiology Spectrum [Category] SARS, 진단, 치료기술, [키워드] Accessibility adoption Asymptomatic biosafety bottleneck capture Care challenge Cold cold chain Collection Community contagious COVID-19 COVID-19 infection COVID-19 patient COVID-19 patients COVID-19 testing COVID-19 transmission diagnostic diagnostic sensitivity diagnostics feasibility highlight identify inactivated India lack limit maintain medium microbial molecular Molecular diagnosis molecular diagnostics neutralize pandemic patients Prevent qPCR qRT-PCR quantitative PCR sample collection SARS-CoV-2 sensitivity significantly lower specimen specimen collection sustained symptomatic temperature threshold Transport transport medium unique unmet need urban population utility Viral Viral transmission Viral transport medium Virology virus VTM [DOI] 10.1128/Spectrum.01108-21 PMC 바로가기 [Article Type] Research Article
Vitality of Proteinase K in rRTPCR Detection of SARS-CoV2 Bypassing RNA ExtractionSARS-CoV2 우회 RNA 추출의 rRTPCR 검출에서 Proteinase K의 활력Cellular and Infection Microbiology Published on 2021-11-032022-09-11 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 치료기술, [키워드] acid Chain Reaction COVID – 19 demonstrated detect detection extraction followed by group heat inactivation inoculated molecular negative sample nucleic acid nucleic acids ORF1 ORF1ab gene polymerase chain polymerase chain reaction positive processed Proteinase K reagent real time PCR real-time polymerase chain reaction RNA RNA extraction SARS-CoV-2 detection SARS-CoV2 sensitivity temperature Template tested three groups treated Treatment Viral vitality VTM were used [DOI] 10.3389/fcimb.2021.717068 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
RNA-extraction-free diagnostic method to detect SARS-CoV-2: an assessment from two states, IndiaOriginal Paper Published on 2021-11-022022-11-01 Journal: Epidemiology and Infection [Category] COVID-19, [키워드] 95% CI 95% confidence interval acute respiratory syndrome agreement approach blinded buffer can be used collected coronavirus disease detect Diagnostic method diagnostic test dry swab evaluated exhibited heat-inactivated heat-inactivation in viral incubated independent India Infection Kolkata medium nasopharyngeal oropharyngeal swab pandemic Performance-evaluation polymerase chain processed protocol reference method RNA extraction RNA-dependent RNA polymerase RNA-extraction-free method SARS-CoV-2 sensitivity Sensitivity and specificity Swab tested Transport Treatment viral gene VTM [DOI] 10.1017/S0950268821002302 PMC 바로가기 [Article Type] Original Paper
Evaluation of the Visby medical COVID-19 point of care nucleic acid amplification testShort communication Published on 2021-11-012022-10-05 Journal: Clinical biochemistry [Category] 진단, 치료기술, [키워드] acute respiratory syndrome analytical sensitivity calculated Characteristics CIconfidence interval claimed collected Concordance confidence interval coronavirus COVID-19 COVID-19 test Cpcrossing point Critical crossing point demonstrated diagnostic Emergency use authorization EUA EUAemergency use authorization evaluated experiment FDAFood and Drug Administration Food and Drug Administration horseradish peroxidase HRPhorseradish peroxidase in viral inactivated institutional review board IRBinstitutional review board Laboratory LIGHT limit of detection LoDlimit of detection Mayo Clinic NAATnucleic acid amplification test nasopharyngeal negative percent agreement NPAnegative percent agreement NPnasopharyngeal nucleic acid amplification nucleic acid amplification test overall concordance Patient Patient care platform POC POCpoint-of-care point of care point-of-care positive positive percent agreement positive sample PPApositive percent agreement qualitative detection Rapid rapid SARS-CoV-2 testing reducing reference method resulting reverse transcription quantitative polymerase chain reaction Roche Roche Diagnostics RT-qPCR RT-qPCRreverse transcription quantitative polymerase chain reaction SARS-CoV-2 SARS-CoV-2 nucleic acid SARS-CoV-2severe acute respiratory syndrome coronavirus 2 serial dilution severe acute respiratory syndrome coronavirus 2 specimen Swab TaqMan Transmission Transport media viral transfer media virus Visby VTM VTMviral transfer media was tested WHOWorld Health Organization widespread World Health Organization [DOI] 10.1016/j.clinbiochem.2021.11.007 [Article Type] Short communication
An ultrafast SARS-CoV-2 virus enrichment and extraction method compatible with multiple modalities for RNA detectionRNA 검출을 위한 다중 양식과 호환되는 초고속 SARS-CoV-2 바이러스 농축 및 추출 방법Randomized Controlled Trial Published on 2021-10-022022-09-11 Journal: Analytica chimica acta [Category] MERS, SARS, 임상, 진단, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus affinity Affinity nanoparticles alternative method alternative methods amplification Analysis Chain Reaction characterization characterized clinical samples clinically comparable compatibility Concordance coronavirus Coronavirus-2 COVID-19 pandemic demonstrated diagnoses Dilution dilutions double-blind study Enzymatic extraction form isothermal amplification Laboratory LAMP Loop-mediated amplification (LAMP) Loop-mediated isothermal amplification miniaturization nasopharyngeal swabs Particle pathogenicity polymerase polymerase chain polymerase chain reaction Polymerase chain reaction (PCR) positive recombinase polymerase amplification Recombinase polymerase amplification (RPA) respiratory reverse transcription reverse transcription real-time polymerase chain reaction RNA RNA eluate RNA isolation RNA virus RPA RT-PCR Saliva SARS-CoV-2 SARS-CoV-2 viral SARS-CoV-2 virus Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus-2 Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) solution Stage the stage transmission rate Viral viral titer viral titers viral transport media VTM zoonotic [DOI] 10.1016/j.aca.2021.338846 PMC 바로가기 [Article Type] Randomized Controlled Trial
Evaluation of the SARS-CoV-2 Inactivation Efficacy Associated With Buffers From Three Kits Used on High-Throughput RNA Extraction PlatformsCellular and Infection Microbiology Published on 2021-09-162022-10-31 Journal: Frontiers in Cellular and Infection Microbiology [Category] COVID-19, MERS, SARS, [키워드] acid addition biosafety Clinical diagnosis clinical samples Combination Culture demonstrated diagnostic Efficacy evaluated extraction guanidinium heat high throughput inactivation infectious dose isopropanol Laboratory laboratory-acquired infection lysis buffer operator PCR platform Qiagen Rapid reduced reduction Result RNA RNA extraction SARS-CoV-2 shown TCID tested the SARS-CoV-2 Thermo Fisher tissue titre Treatment viral RNA extraction Viral transport medium virus Volume VTM [DOI] 10.3389/fcimb.2021.716436 PMC 바로가기 [Article Type] Cellular and Infection Microbiology
Sensitivity of Rapid Antigen Testing and RT-PCR Performed on Nasopharyngeal Swabs versus Saliva Samples in COVID-19 Hospitalized Patients: Results of a Prospective Comparative Trial (RESTART)Article Published on 2021-09-092022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] Antigen applied approach assays Basel biosensor collected Comparative conducted COVID-19 COVID-19 diagnosis Diagnosis diagnostic duration of symptom exhibited help Hospitalized hospitalized patient hospitalized patients intensive care investigated IQR mitigate much lower nasopharyngeal Nasopharyngeal swab Non-invasive Patient Prospective prospective observational study Rapid rapid antigen test rapid antigen testing RAT RATs Result RT-PCR RT-PCR testing Saliva salivary Sample SARS-CoV-2 SARS-CoV-2 diagnosis sensitivity shortage of reagent specificity specimen Standard Q step Swab swabs Switzerland Test Testing the median Viral transport medium VTM was used [DOI] 10.3390/microorganisms9091910 PMC 바로가기 [Article Type] Article
Sample collection and transport strategies to enhance yield, accessibility, and biosafety of COVID-19 RT-PCR testingEditor's Choice Published on 2021-09-062022-10-29 Journal: Journal of Medical Microbiology [Category] COVID-19, SARS, [키워드] biosafety buffer collected combined use COVID-19 COVID-19 testing diagnostic diagnostics Direct eNAT ENhance evaluate Gap inactivation nasal nasal swab nasal swabs nasopharyngeal Non-invasive NP swab oral oral swab oral swabs patients point-of-care polymerase chain positive Result RT-PCR RT-PCR testing Safe Saliva sample collection SARS-CoV-2 sensitivity significantly more specimen Swab swabs Transport viral transport media VTM widespread [DOI] 10.1099/jmm.0.001380 PMC 바로가기 [Article Type] Editor's Choice