Inhibitor screening using microarray identifies the high capacity of neutralizing antibodies to Spike variants in SARS-CoV-2 infection and vaccination마이크로어레이를 사용한 억제제 스크리닝은 SARS-CoV-2 감염 및 백신 접종에서 스파이크 변이체에 대한 항체를 중화하는 높은 능력을 식별합니다Article Published on 2022-02-282022-09-11 Journal: Theranostics [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] addition anti-S antibody antibody antibody diversity B.1.1.7 B.1.351 B.1.617.2 Cohort convalescent COVID-19 patient convalescent COVID-19 patients coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 vaccine COVID-19 vaccines D614G Drug development Efficacy FIVE glass slides group help heterogeneous identify Microarray Mutation NAb titers NAbs neutralization Neutralization assay neutralize Neutralizing antibodies neutralizing antibody neutralizing effect P.1 peptide peptide microarray platform Population prevalent protective effect Protein purified reduce responsible S variant SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 spike Screening serum spike spike-ACE2 These data utility vaccination vaccinee vaccinees variant variants were assessed wild-type [DOI] 10.7150/thno.67038 PMC 바로가기 [Article Type] Article
Co-circulation of two SARS-CoV-2 variant strains within imported pet hamsters in Hong Kong홍콩에서 수입된 애완용 햄스터 내에서 두 개의 SARS-CoV-2 변종 균주의 공동 순환Coronaviruses Published on 2022-02-272022-09-10 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] adaptive animal animals aviation binding energy can not coronavirus COVID-19 Delta delta variant epidemiologically Europe European hamster hamsters Hong Kong Human human ACE2 interspecies molecular docking Netherland Netherlands outbreak Outbreaks Patient phylogenetically positive Prevent RBD reported respiratory Respiratory Coronavirus risk SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant SARS-CoV-2 variants severe acute respiratory coronavirus spike mutation spike receptor-binding domain spike variant spike variants strain Strains subsequent suggested susceptible Testing these viruses Transmission triggered variant virus viruses warehouse while zoonosis [DOI] 10.1080/22221751.2022.2040922 PMC 바로가기 [Article Type] Coronaviruses
Durable T-cellular and humoral responses in SARS-CoV-2 hospitalized and community patientsSARS-COV-2 입원 및 지역 사회 환자의 내구성 T- 세포 및 체액 반응Clinical Trial Published on 2022-02-222022-09-10 Journal: PLoS ONE [Category] COVID19(2023년), MERS, SARS, 변종, 임상, 진단, 치료기술, [키워드] age analyzed antibody Antibody Response Antibody responses assays Blood blood sample Blood samples boost Clinical data clinical observations collected Community Comorbidity determine diagnosed ELISA Gender Hospitalized Humoral response IFN-γ IgA IgG IgM IL-2 immune response Immunity immunosorbent impacted infected patients Infection interferon interferon-γ interleukin investigated long-term memory memory response microneutralization mononuclear cell mononuclear cells neutralization Neutralizing antibodies neutralizing antibody pandemic Patient patients peripheral Peripheral blood Peripheral blood mononuclear cells prospective cohort Protein pseudotype Re-infection recruited response Result risk RT-PCR SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 virus secreting serum sample serum samples severity severity of illness specific antibodies specific antibody T-cell Response T-cell responses vaccination variant variants of concern virus-specific T-cell response virus-specific T-cell responses viruses [DOI] 10.1371/journal.pone.0261979 PMC 바로가기 [Article Type] Clinical Trial
Classical and Next-Generation Vaccine Platforms to SARS-CoV-2: Biotechnological Strategies and Genomic VariantsSARS-CoV-2에 대한 고전 및 차세대 백신 플랫폼: 생명공학 전략 및 게놈 변이Review Published on 2022-02-182022-09-10 Journal: International Journal of Environmental Research an [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, [키워드] aAPC Ad5-nCoV adaptive Amino acid amino acids animals antibody applied B.1.1.7 B1.351 BBIBP-CorV bind Biotechnology BNT Cell classical clinical trial consequence CoronaVac coronavirus COVID-19 COVID-19 vaccine COVID-19 vaccines CoVs D614G Deletion demonstrated dendritic cell dendritic cells domain escape Evolution genomic variant genomic variants HCoV HCoV-229E HCoV-HKU1 HCoV-NL63 HCoV-OC43 high affinity HKU1 hosts Human humans inactivated incorporation inhibit INO-4800 JNJ-78436735 lentiviral Lentiviral vector LV-SMENP-DC MERS-CoV molecular mRNA mRNA-1273 mutated nCoV New new SARS-CoV-2 new strain NL63 NVX-CoV2373 vaccine P.1 pathogen Pathogens platform Protein RBD receptor ACE2 Receptor-binding domain recombinant vaccine recombinant vaccines replacement residue responsible RNA vaccines S protein SARS-CoV SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variants strain Strains Strategy subunits technological platforms tested the S protein Transmissibility trials Vaccine vaccine production Vaccines variant variants variety Viral virus-like particles β-coronavirus β-CoV β-CoVs [DOI] 10.3390/ijerph19042392 PMC 바로가기 [Article Type] Review
Computer simulations on oxidative stress-induced reactions in SARS-CoV-2 spike glycoprotein: a multi-scale approachOriginal Article Published on 2022-02-182022-10-30 Journal: Molecular diversity [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] Abstract antioxidant approach attacked caused Computer simulation conducted disease driven by drugs expected help Hydrogen abstraction in silico isoleucine Modification molecular dynamics occur organism oxidative oxidative stress pathogenicity performed reaction Reactions reactive reactive oxygen species reactive oxygen specy Receptor binding domain residue resulting risk factor SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike glycoprotein Spike protein Stress susceptible therapeutic triggered virus [DOI] 10.1007/s11030-021-10373-6 PMC 바로가기 [Article Type] Original Article
A novel isothermal method for amplification of long specific amplicon from linear templateArticle Published on 2022-02-172022-10-29 Journal: Scientific Reports [Category] COVID-19, [키워드] amplification analyzed applied Biological techniques can be used Cre recombinase CREA diagnostic DNA downstream evaluated generate genomic isothermal limit linear nucleic acid amplification Pathogens RCA Recombination RNA RNA templates SARS-CoV-2 spike sequence subsequent Surveillance Variation [DOI] 10.1038/s41598-022-06785-5 PMC 바로가기 [Article Type] Article
Increased Expression of Tim-3 Is Associated With Depletion of NKT Cells In SARS-CoV-2 InfectionTim-3의 발현 증가는 SARS-CoV-2 감염에서 NKT 세포의 고갈과 관련이 있습니다Immunology Published on 2022-02-162022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition Analysis Apoptosis binding caspase caused CD147 CD26 Cell circulating coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patients CTLA-4 DCs decrease demonstrated dendritic cell dendritic cells Depletion disease severity dysfunction expressed expression expression level Genes healthy individuals IFN-γ IL-10 IL-12 IL-4 immune responses Immunopathogenesis implication Increased increased expression LAG-3 large cohort mechanism mitochondrial mitochondrial gene mitochondrial genes natural killer NKT NKT cell NKT cells outcome patients with COVID-19 PD-1 positively correlated Prognosis progression stage promote receptor receptors respiratory SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike scRNA-seq secrete secreted Sequencing analysis severe acute respiratory syndrome Coronavirus severity and prognosis Single-cell RNA sequencing stages therapeutic strategy TIM-3 up-regulated [DOI] 10.3389/fimmu.2022.796682 PMC 바로가기 [Article Type] Immunology
Randomized, Double Blind, Placebo Controlled, Clinical Trial to Study Ashwagandha Administration in Participants Vaccinated Against COVID-19 on Safety, Immunogenicity, and Protection With COVID-19 Vaccine–A Study ProtocolMedicine Published on 2022-02-162022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] administration Against All adverse event antibody titres approval Ashwagandha Ayurveda benefit clinical commenced Controlled COVID-19 COVID-19 infection COVID-19 vaccine double-blind effective Ethics exploratory study healthy volunteer immunoadjuvant immunogenicity immunological India Indian people interim analysis margin Measures Medicine National offered outcome measure Oxford-AstraZeneca parallel-group participant peer-reviewed placebo-controlled populations power calculation protection protective immune response Quality of life Randomized RBD-specific IgG receiving recruited recruitment regimen Registration reported Result Safety SARS-CoV-2 spike second dose secondary Two-arm vaccinated against COVID-19 vaccination Vaccine was obtained Withania somnifera [DOI] 10.3389/fmed.2022.761655 PMC 바로가기 [Article Type] Medicine
Quantum dots assembly enhanced and dual-antigen sandwich structured lateral flow immunoassay of SARS-CoV-2 antibody with simultaneously high sensitivity and specificitySARS-CoV-2 항체의 양자점 조립이 강화되고 이중 항원 샌드위치 구조의 측면 유동 면역분석이 동시에 높은 감도와 특이성을 나타냄Article Published on 2022-02-152022-09-12 Journal: Biosensors & bioelectronics [Category] Coronavirus, COVID19(2023년), MERS, SARS, 진단, [키워드] amplification antibody Antigen applied approach characteristic chemiluminescence immunoassay CLIA COVID-19 COVID-19 negative dendritic Diagnosis Dual-antigen sandwich immunoassay False negative rate false positive rate false-negative False-positive fluorescent groups human SARS-CoV-2 hydrophobic indicated Interpretation lateral flow immunoassay LFIA limitations Misinterpretation over overcome Patient PCR POC point-of-care point-of-care testing polymerase chain Population screening positive public health Quantitative Quantum dot Quantum dots assembly Receptor binding domain reduce resource risk robust SARS-CoV-2 antibody SARS-CoV-2 spike sensitivity Sensitivity and specificity serum signal signal amplification specificity statistical analysis viruses [DOI] 10.1016/j.bios.2021.113810 PMC 바로가기 [Article Type] Article
Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoprotein합성 비리온은 SARS-CoV-2 스파이크 당단백질의 지방산 결합 적응 면역원성을 나타냅니다.Article Published on 2022-02-142022-09-10 Journal: Nature Communications [Category] COVID19(2023년), MERS, SARS, 변종, 유전자 메커니즘, 진단, 치료기술, 치료법, 치료제, [키워드] adaptation adaptive binding binding pocket Bioinspired materials Cell conserved convalescent COVID-19 COVID-19 progression Dexamethasone Donor fatty Fatty acid fatty acids FDA-approved drug FFAs free fatty acid function global public health glycoprotein heterogenous Host immune response immunogenicity Immunoglobulin immunoglobulins Inflammation Inflammatory integrin Live virus mechanism mimicking modulate molecular motif Mutant SARS-CoV-2 neutralization activity Neutralizing open Pathogenesis preparation pro-inflammatory proinflammatory protocol RBD Receptor binding Receptor binding domain Regulation Regulatory responsible reveal SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike SARS-CoV-2 spike glycoprotein synthetic the SARS-CoV-2 Variability Viral virion vitamin K wild-type [DOI] 10.1038/s41467-022-28446-x PMC 바로가기 [Article Type] Article