Evaluation of a real-time RT-PCR panel for detection of SARS-CoV-2 in bat guano박쥐 구아노에서 SARS-CoV-2 검출을 위한 실시간 RT-PCR 패널 평가Article Published on 2021-03-012022-09-11 Journal: Journal of veterinary diagnostic investigation : o [Category] SARS, 조류인플루엔자, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus agency animals bat bat guano bats cats causative agent coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 detect evaluated Health Human human disease implicated lead mink natural reservoir North Novel coronavirus outbreak populations Real-time PCR real-time RT-PCR rehabilitation facility respiratory resulting risk RT-rtPCR SARS-CoV-2 SARS-CoV-2 viral RNA severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 transfer transmitted Viral virus [DOI] 10.1177/1040638721990333 PMC 바로가기 [Article Type] Article
Clinical Usefulness of LabChip Real-time PCR using Lab-On-a-Chip Technology for Diagnosing Malariaarticle Published on 2021-02-282024-09-01 Journal: The Korean Journal of Parasitology [Category] 말라리아, [키워드] lab chip malaria microfluidic Plasmodium Real-time PCR [Article Type] article
Administration of zoledronic acid alleviates osteoporosis in HIV patients by suppressing osteoclastogenesis via regulating RANKL expressionzoledronic acid 투여는 RANKL 발현 조절을 통해 파골세포 형성을 억제함으로써 HIV 환자의 골다공증 완화Research Article Published on 2021-02-262022-09-06 Journal: Molecular Medicine [Category] Communicable Disease, [키워드] 3′ UTR activated activating administration alleviate binding carried demonstrated down-regulated ELISA evaluate expression expression of gene HIV HIV patient HIV patients inhibitory effect Inhibitory effects involved JNK luciferase Luciferase assays mechanism miR-101 miR-145 miRNA modulating osteocalcin osteoclast Osteoclastogenesis Osteoporosis Pathways patients PRKACB quantitative real-time PCR RANKL Real-time PCR restored Result serum signaling pathway signaling pathways significantly Smad3 subject suppressed tenofovir treated Treatment was performed was used western blot Zoledronic acid [DOI] 10.1186/s10020-021-00276-5 PMC 바로가기 [Article Type] Research Article
Genome Sequence of a Minacovirus Strain from a Farmed Mink in The NetherlandsGenome Sequences Published on 2021-02-252022-10-29 Journal: Microbiology Resource Announcements [Category] COVID-19, [키워드] acute respiratory syndrome coronavirus deep sequencing fecal genome sequence mink Neovison Netherland Real-time PCR RT-PCR SARS-CoV-2 strain viral genome sequence was obtained was tested [DOI] 10.1128/MRA.01451-20 PMC 바로가기 [Article Type] Genome Sequences
SARS-CoV-2 Direct Detection Without RNA Isolation With Loop-Mediated Isothermal Amplification (LAMP) and CRISPR-Cas12Medicine Published on 2021-02-172022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] clinical sample confirmed case COVID-19 COVID-19 outbreak CRISPR-Cas12a current detection Diagnosis diagnostic diagnostic protocol Direct epidemiological equipment evaluated faster LAMP limit of detection Loop-mediated isothermal amplification management Palindromic Patient Protein qRT-PCR Real-time PCR reported RNA RNA extraction SARS-CoV-2 Sensitivity and specificity specificity thermocycler Viral RNA [DOI] 10.3389/fmed.2021.627679 PMC 바로가기 [Article Type] Medicine
Honeysuckle Aqueous Extracts Induced let-7a Suppress EV71 Replication and Pathogenesis In Vitro and In Vivo and Is Predicted to Inhibit SARS-CoV-2Article Published on 2021-02-162022-10-28 Journal: Viruses [Category] COVID-19, [키워드] let-7a accompanied aqueous attenuate cell line cells Chinese demonstrated dengue virus endogenous EV71 Evidence examined expressed expression Extract honeysuckle Human in vitro in vivo indicate induce induced ingestion innate response inoculated investigated Lonicera japonica luciferase activity luciferase reporter plasmid mice miRNA miRNA expression miRNAs non-coding RNA overexpressed overlapped pathogen invasion Pathogenesis Plaque assay predicted Prevent profile protein expression Real-time PCR Replication reporter RNA RNA genome RT-PCR SARS-CoV-2 sequence suppress suppressive function survival TCM upregulated upregulation Viral load viral replication Viral RNA virus replication virus titer western blotting [DOI] 10.3390/v13020308 PMC 바로가기 [Article Type] Article
Pooled testing with replication as a mass testing strategy for the COVID-19 pandemicsCOVID-19 전염병에 대한 대규모 테스트 전략으로 복제를 사용한 통합 테스트Article Published on 2021-02-102022-09-01 Journal: Scientific Reports [Category] MERS, 진단, 치료기술, [키워드] Applied mathematics approach average conjunction COVID-19 COVID-19 pandemic detect extraction filament group Infection Mild nasopharyngeal swabs Oropharyngeal outbreak Pandemics Patient present reactants Real-time PCR reduce reduction Replication RNA Sequencing Swab swabs tested viral infection virus [DOI] 10.1038/s41598-021-83104-4 PMC 바로가기 [Article Type] Article
Verification and Validation of SARS-CoV-2 Assay Performance on the Abbott m 2000 and Alinity m SystemsAbbott m 2000 및 Alinity m 시스템에서 SARS-CoV-2 분석 성능 검증 및 검증Comparative Study Published on 2021-02-102022-09-10 Journal: Journal of Clinical Microbiology [Category] MERS, SARS, 진단, 치료기술, [키워드] 2019-nCoV 95% CI 95% confidence interval Abbott average CDC collected Control correlated COVID-19 diagnostic limit of detection linearity nasopharyngeal NP swab performance performed positive positive sample R 2 Real-time PCR Reference materials reproducibility RNA detection RT-PCR Saline SARS-CoV-2 specimen swab specimen System TaqPath tested Thermo Thermo Fisher validation verification were used [DOI] 10.1128/JCM.03119-20 PMC 바로가기 [Article Type] Comparative Study
COVID-19 Home Deaths without Medical Assistance in Northeastern BrazilArticles Published on 2021-02-102022-10-28 Journal: The American Journal of Tropical Medicine and Hygi [Category] COVID-19, MERS, [키워드] age assistance Autopsy average Brazil cardiovascular disease caused Characteristics Comorbidity conducted confirmed case cough COVID-19 COVID-19 in patient death diabete diagnosed Dyspnea epidemiological Evidence fatigue Fever health system health systems home lack male nasopharyngeal swabs Neurological disease pandemic Patient Prevalence reached Real-time PCR reported retrospective statistically Symptom the disease verbal Wuhan, China [DOI] 10.4269/ajtmh.20-1210 PMC 바로가기 [Article Type] Articles
Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 PatientsCell and Developmental Biology Published on 2021-02-052022-10-31 Journal: Frontiers in Cell and Developmental Biology [Category] COVID-19, [키워드] activated acute respiratory syndrome co-culture Concentration conditions Contact coronavirus coronavirus disease COVID-19 COVID-19 patient COVID-19 patients cytokine dental dental pulp stem cells deregulated DPSC DPSCs form GM-CSF granulocyte healthy subject Human hyper-inflammation IFNγ IL-10 IL-12 IL-13 IL-17A IL-18 IL-1β IL-2 IL-21 IL-22 IL-23 IL-27 IL-4 IL-5 IL-6 IL-9 immune response immunomodulation in vitro interleukin investigated Macrophage mechanism mesenchymal stem cell Mesenchymal stem cells modulate modulation mononuclear cell MSC MSCs Multi-organ damage not affected Patient PBMC PBMCs peripheral Peripheral blood Pneumonia Production promote Pulp Real-time PCR Result SARS-CoV-2 STEM stem cell subset supernatant tissue regeneration TNFα [DOI] 10.3389/fcell.2020.609204 PMC 바로가기 [Article Type] Cell and Developmental Biology