Sensitive and Specific Detection of SARS-CoV-2 Antibodies Using a High-Throughput, Fully Automated Liquid-Handling Robotic System처리량이 완전 자동화된 액체 처리 로봇 시스템을 사용한 SARS-CoV-2 항체의 민감하고 특이적인 검출Original Research Published on 2020-08-202022-09-01 Journal: Slas Technology [Category] MERS, SARS, 진단, 치료기술, [키워드] addition agglutination analyst anti–spike protein antibody Antibody detection Antigen automated battle can be used Community Contact contact with control patient Coronaviral coronavirus disease Coronavirus disease 2019 CoV-NL63 COVID-19 cross-react detection generate global pandemic helping immune complex immunoassay individual infected patients Infection infection prevalence infections liquid handling pandemic Patient PCR PCR-confirmed COVID-19 patient platform probe protocol qualitative detection quarantine reduce responsible SARS-CoV-2 sensitive sensitivity serological assays serum Specific specificity Spike protein STAR System tested total antibodies total antibody Treatment virus [DOI] 10.1177/2472630320950663 PMC 바로가기 [Article Type] Original Research
Impact of comprehensive molecular testing to reduce antibiotic use in community-acquired pneumonia (RADICAP): a randomised, controlled, phase IV clinical trial protocol지역사회획득 폐렴(RADICAP)에서 항생제 사용을 줄이기 위한 포괄적인 분자 테스트의 영향: 무작위, 통제된, IV상 임상 시험 프로토콜Article Published on 2020-08-202022-09-11 Journal: BMJ Open [Category] MERS, 임상, 진단, [키워드] 1:1 48 hour 48 hours Admission adverse events antibiotic Antibiotic therapy Antibiotic treatment antibiotic use Antibiotics antimicrobial resistance assigned both group both groups causative agent clinical trial ClinicalTrials community-acquired pneumonia De-escalation death Device Diagnosis diagnostic Diagnostic microbiology effective eligible Endpoint Escalation Ethical approval Ethics ethics committee Health health problem hospital hospitalised Hypothesis Immunosuppressed Impact include intensive care intensive care unit admission International intravenous mechanical ventilation Medical devices Medicine molecular molecular diagnostics null hypothesis Open-label parallel group Patient patients Pneumonia primary endpoint Probability protocol randomisation randomised Randomly readmission receive reduce reducing registration number respiratory respiratory infections Result resulting secondary Secondary endpoints Side effects Spanish stability subject [DOI] 10.1136/bmjopen-2020-038957 PMC 바로가기 [Article Type] Article
The Psychosocial Effects of the COVID-19 Pandemic on Youth Living with HIV in Western KenyaOriginal Paper Published on 2020-08-202022-10-27 Journal: AIDS and Behavior [Category] Coronavirus, [키워드] administer Adolescent age Care COVID-19 Depression depressive symptom Differentiated care Effect feasibility Follow-up group had more Health HIV Kenya majority medication mental health Mild mild depression offer Offering participant PHQ-9 protocol Psychosocial question reported Support Symptoms with HIV Youth [DOI] 10.1007/s10461-020-03005-x PMC 바로가기 [Article Type] Original Paper
The Psychosocial Effects of the COVID-19 Pandemic on Youth Living with HIV in Western Kenya서부 케냐에서 HIV에 감염된 청소년에 대한 COVID-19 팬데믹의 심리사회적 영향Original Paper Published on 2020-08-202022-09-10 Journal: AIDS and Behavior [Category] 치료제, [키워드] administer Adolescent age being Care COVID-19 Depression depressive depressive symptom Depressive symptoms Differentiated care Effect feasibility financial support Follow-up group had more Health Health care HIV Kenya majority Medical care medication mental health mental health care Mild mild depression offer Offering participant PHQ-9 protocol Psychosocial question reported Support Symptoms telephone with HIV Youth [DOI] 10.1007/s10461-020-03005-x PMC 바로가기 [Article Type] Original Paper
COVID-19 Intubation Safety: A Multidisciplinary, Rapid-Cycle Model of ImprovementArticle Published on 2020-08-182022-10-27 Journal: American journal of medical quality : the official [Category] COVID-19, [키워드] Analysis can be used Care checklist Compliance COVID-19 COVID-19 pandemic COVID-19 patient demonstrated develop ENhance Health improvement Improvements intubation intubations Model Personal protective equipment process improvement protocol protocols Quality improvement risk Safe Safety SARS-CoV-2 [DOI] 10.1177/1062860620949141 PMC 바로가기 [Article Type] Article
COVID-19 Intubation Safety: A Multidisciplinary, Rapid-Cycle Model of ImprovementArticle Published on 2020-08-182022-10-27 Journal: American journal of medical quality : the official [Category] Coronavirus, COVID-19, MERS, SARS, [키워드] Analysis can be used Care checklist Compliance COVID-19 COVID-19 pandemic COVID-19 patient demonstrated develop ENhance Health improvement Improvements intubation intubations Model Personal protective equipment process improvement protocol protocols Quality improvement risk Safe Safety SARS-CoV-2 [DOI] 10.1177/1062860620949141 PMC 바로가기 [Article Type] Article
Direct-RT-qPCR Detection of SARS-CoV-2 without RNA Extraction as Part of a COVID-19 Testing Strategy: From Sample to Result in One HourArticle Published on 2020-08-182022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] 2019-nCoV absence acute respiratory syndrome Antiviral approach BSL-2 comparable condition coronavirus Coronavirus disease 2019 costly COVID-19 COVID-19 infection COVID-19 testing Critical Cycling detect detection diagnostic diagnostics effective enzyme extraction General population heat inactivation help hour Implementing include involved Laboratory lack less limit of detection Local media non-infectious one-step direct RT-PCR pandemic Part performed point-of-care point-of-care testing protective immunity protocol required Result RNA RNA extraction RNA isolation RT-qPCR Sample SARS-CoV-2 screening tests sensitivity Sensitivity and specificity Seven Spread suggested Swab Testing the disease time-consuming transfer translate Vaccines virus [DOI] 10.3390/diagnostics10080605 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Detection for Diagnosis Purposes in the Setting of a Molecular Biology Research LabArticle Published on 2020-08-182022-10-30 Journal: Methods and Protocols [Category] COVID-19, [키워드] amplification Analysis Asymptomatic carrier automated Biology clinical sample composed contagious COVID-19 COVID-19 case Critical detection Diagnosis diagnostic false-negative growth heterogeneous Isolation lab lack Molecular biology nucleic acid extraction PCR platform protocol public health systems reagent Research researchers reverse transcription RTqPCR SARS-CoV-2 SARS-CoV-2 detection setting specimens the SARS-CoV-2 virus viral nucleic acids [DOI] 10.3390/mps3030059 PMC 바로가기 [Article Type] Article
Multi-centre, three arm, randomized controlled trial on the use of methylprednisolone and unfractionated heparin in critically ill ventilated patients with pneumonia from SARS-CoV-2 infection: A structured summary of a study protocol for a randomised controlled trialLetter Published on 2020-08-172022-10-29 Journal: Trials [Category] Coronavirus, MERS, [키워드] 24 hour absolute reduction Administered administration AIFA airway allocation sequence Alpha Anticoagulant anticoagulant therapy Anticoagulant treatment approval Arm Aspergillus assigned assumption average bleeding bleeding event blinded Block randomisation block size BMI Brain Breastfeeding Candida checked childbearing age chronic clinical clinical condition clinically combined use conjunctival contributed corpus Corticosteroid COVID-19 criteria Critical Critically ill cytomegalovirus D-dimer level death defined Delay detect diagnostic discharge dissemination dosage drug dysfunction effective element eligible patient enoxaparin enrolled EudraCT exclusion criteria expected hematemesis hematoma heparin-induced thrombocytopenia Herpe high risk High-dose history hospital hospital discharge Hypothesis ICU ICU free day ICU Patients impairing Inclusion inclusion criteria indicated Infection initial Inpatient intensive care unit interval Intervention Intra-articular intramuscular intravenously invasive Invasive mechanical ventilation investigator Italian limit LMWH low molecular weight low-molecular weight heparin macroscopic major bleeding mechanical ventilation medical intervention Methylprednisolone Mortality mortality rate Myocardial Non-invasive non-invasive ventilation number objective occur ophthalmic organ dysfunction PaO parallel group participant Patient performed Pharyngeal swab Pneumonia positive positive pressure pregnancy test Presence Prophylactic prophylactic dose Prophylaxis protocol random randomisation randomised randomised controlled trial randomization Randomized Randomized controlled trial Randomly Ratio receive recent recruited recruitment Rectal Bleeding red blood cell reduce reducing Registered required risk of death Safety Sample size SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 pneumonia secondary secure severe disease sick significantly sodium status steroid stratified stroke study drug Study protocol subcutaneously surgical syndrome therapeutic therapeutic doses therapy Treatment treatment group unfractionated heparin Venous Thromboembolism ventilated patient Ventilation website Weighting Withdrawal women [DOI] 10.1186/s13063-020-04645-z PMC 바로가기 [Article Type] Letter
Whole Genome Sequencing of SARS-CoV-2: Adapting Illumina Protocols for Quick and Accurate Outbreak Investigation during a PandemicArticle Published on 2020-08-172022-10-31 Journal: Genes [Category] COVID-19, MERS, [키워드] acute respiratory syndrome Analysis bioinformatics can be used coronavirus COVID-19 COVID-19 pandemic Degradation Genome Genomics Genotype genotyping hospital identify Illumina IMPROVE Infection Infection prevention investigation Laboratory manuscript network outbreak problem protocol protocols reagents SARS-CoV-2 SARS-CoV2 Sequencing South Africa Spread Transmission viral genomes [DOI] 10.3390/genes11080949 PMC 바로가기 [Article Type] Article