Development of a recombinant Newcastle disease virus-vectored vaccine for infectious bronchitis virus variant strains circulating in EgyptResearch Article Published on 2019-02-112022-10-28 Journal: Veterinary Research [Category] Coronavirus, [키워드] analyses avirulent bronchitis cause chick circulating circumvent clinical disease Clinical sign comparable Complete develop development disease Express expressing growth IBV immunofluorescence in vitro Infectious Bronchitis Virus Lineage live-attenuated vaccine Middle East mutate newcastle disease virus parental prevalent provided recombine reduced reduction in resulting S glycoprotein S protein S protein expression significantly Single-dose SPF strain supplementary material tracheal vaccination Vaccine vaccine candidate variant viral shedding virulent virus viruses were expressed white wild type [DOI] 10.1186/s13567-019-0631-5 PMC 바로가기 [Article Type] Research Article
Broad antifungal resistance mediated by RNAi-dependent epimutation in the basal human fungal pathogen Mucor circinelloides기초 인간 곰팡이 병원체 Mucor circinelloides에서 RNAi 의존성 후성 돌연변이에 의해 매개되는 광범위한 항진균제 내성Research Article Published on 2019-02-112022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] adaptation Analysis antifungal agent antifungal agents antifungal drug resistance cause clinically demonstrated develop drug drug resistance drug target drugs enzyme Enzymes Evolution FK506 fungal Fungal infection fungi Genes high mortality IMPROVE induce Infection mechanism Mucor Mucormycosis Mutation novel passage pathogen Pathogenesis Proteins Rapamycin resistance mechanism response restored reversion RNA RNA expression RNA interference RNAi RNAi pathway selected sensitivity small RNA sRNA strain target gene treat Treatment variety [DOI] 10.1371/journal.pgen.1007957 PMC 바로가기 [Article Type] Research Article
Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle trackingArticle Published on 2019-02-042022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] addition Analysis cause Cell cell membrane Characteristics contagious cytoplasm disease epidemic diarrhea host cells infection mechanism live cells mechanism microtubule organizing center PEDV performed provided Real-time analysis Transport Vero cells virus [DOI] 10.1038/s41598-018-37789-9 PMC 바로가기 [Article Type] Article
Effect of Pullet Vaccination on Development and Longevity of ImmunityArticle Published on 2019-02-022022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] Administered administration age Antibody Response Antibody titer cause ciliostasis Clinical signs determine development disease Effect evaluated group Histopathology homologous IBV Immunity Infectious Bronchitis Virus Infectious laryngotracheitis virus information interfere Live attenuated vaccine Longevity newcastle disease virus occur pathogen PROTECT protective immunity Respiratory disease respiratory pathogen respiratory tract serum tracheal vaccination Vaccination program Vaccine Vaccines variety Viral load virus [DOI] 10.3390/v11020135 PMC 바로가기 [Article Type] Article
Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation포유류 Hbs1L 결핍은 Pelota 고갈 및 80S 모노솜 축적과 관련된 선천적 기형 및 발달 지연을 유발합니다Research Article Published on 2019-02-012022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] 4EBP1 accompanied accumulation addition affecting aggregation Anomaly baseline biallelic mutation biallelic mutations binding cause caused Cell cells Characteristics characterized coding region conditions control mechanisms controls Critical deficiency demonstrated Depletion developmental delay downstream Effect Efficiency element expression facial fibroblast fibroblasts functional growth Hbs1L human cell human cells humans hypotonia implicated increase in inhibition involved knockdown lack lead Loss mechanism mice monosome mouse model mRNA mRNA level mTOR mTOR expression murine Mutation ortholog pathway Patient pelo phenotype phenotypic Phosphorylation polysome proteasomal proteasome Protein protein expression Quality control reduced Regulation reported rescued restored resulting ribosomal RNA ribosome ribosomes RNA RT-PCR Sequencing Support the patient These data tissues transcript translation translational upregulation utilization western blot Western blot analyses Western blot analysis yeast [DOI] 10.1371/journal.pgen.1007917 PMC 바로가기 [Article Type] Research Article
Exposure of Candida albicans β (1,3)-glucan is promoted by activation of the Cek1 pathwayCek1 경로의 활성화에 의해 칸디다 알비칸스 β(1,3)-글루칸의 노출이 촉진됩니다Research Article Published on 2019-01-312022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] activate activated Activation addition AIDS antigenic approach biogenesis bloodstream Cancer patients Candida Candida albican Candida albicans cascades cause Cdc42 Cell cell wall cell wall synthesis decrease detect disrupt Disruption downstream drug exhibit exposure Fungal infection fungal infections fungal pathogens glucan glycosylated GTPase homozygous Hyperactivation immune immune cells immune function immune system increase independent innate immune cell Innate immune cells Intracellular kinase kinases lab lead leads mannan MAPK masking mitogen Mortality mutant Mutation Pathogens pathway Patient Phosphatidylserine polysaccharide promoted protein kinase receptor receptors regulate remodeling responsible Signaling signaling cascade significantly increased synthesis the cell up-regulated upregulated upregulation upstream virulence virulence factor weakened were used wild-type wild-type cell wild-type cells [DOI] 10.1371/journal.pgen.1007892 PMC 바로가기 [Article Type] Research Article
Heme peroxidase HPX-2 protects Caenorhabditis elegans from pathogensHeme peroxidase HPX-2는 Caenorhabditis elegans를 병원체로부터 보호합니다Research Article Published on 2019-01-292022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] addition Author Caenorhabditis elegan Caenorhabditis elegans catalytic cause changes in characterization complexity component conditions contribute Defense demonstrated Disruption DNA domain downstream elegan elegans ENCODE enzyme exhibited expression fluorescent function generate genes encoding greater heme Host host defense host tissue hydrogen hydrogen peroxide hypodermis indicated Infection Infectious disease Infectious diseases information Innate immunity intestinal involved labeling limit mammalian mechanism microbe microbial microbial infection morbidity morbidity and mortality Morphological mutant NADPH NADPH oxidase observation organ oxygen pathogen pathogenic Pathogens pathway peroxidase peroxidases pharynx previous study PROTECT Protective Reactions reactive oxygen species regulated required residue RNA interference RNA-seq analysis RNAi ROS ROS production shown survival therapy tissue worm [DOI] 10.1371/journal.pgen.1007944 PMC 바로가기 [Article Type] Research Article
Single Chain Fragment Variable (scFv) Antibodies Targeting the Spike Protein of Porcine Epidemic Diarrhea Virus Provide Protection against Viral Infection in PigletsArticle Published on 2019-01-142022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] Administered administration antibody Antigen cause chain cloned contagious coronavirus death diarrhea effective epidemic diarrhea epitope expression fragment higher affinity IFA Infection inhibit intestinal investigated lesions less mild clinical symptoms Mortality Neonatal neutralizing antibody passive immunization PEDV plaque reduction neutralization Plasmid Porcine epidemic diarrhea virus protection protein expression scFv screened selected shown significantly significantly increased single chain fragment variable (scFv) spike (S) protein Support survival rate targeting the Spike the spike protein Treatment variable viral shedding virus [DOI] 10.3390/v11010058 PMC 바로가기 [Article Type] Article
Pathogenicity and immunogenicity of attenuated porcine epidemic diarrhea virus PC22A strain in conventional weaned pigsResearch Article Published on 2019-01-112022-10-28 Journal: BMC veterinary research [Category] Coronavirus, MERS, [키워드] adjuvant Administered age antibody Antibody Response cause caused Cell culture cells diarrhea Efficacy elicit epidemic diarrhea evaluated fecal genetics greater group IgA IgG inactivated induce inoculated intestinal Live attenuated vaccine mortality rate mucosal immune mucosal immunity multiple dose naïve Neonatal Neutralizing PEDV porcine epidemic diarrhea virus (PEDV) post inoculation pregnant priming regimen response Result resulting RNA Safe serum subunit Subunit vaccine supplementary material tested The United States vaccination Vaccine vaccine candidates Viral RNA Viral RNA shedding virulent virus Weaned pigs [DOI] 10.1186/s12917-018-1756-x PMC 바로가기 [Article Type] Research Article
Comparative features of infections of two Massachusetts (Mass) infectious bronchitis virus (IBV) variants isolated from Western Canadian layer flocksResearch Article Published on 2018-12-102022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] bronchitis cause characterized Comparative cross protection distribution elicited evaluated Evidence feature genetic difference Genetic variations Genome host response host responses IBV Infection Infectious Bronchitis Virus isolate isolates lung M41 Macrophage Macrophage response macrophages mass Massachusett morbidity Mortality nucleotide nucleotide sequence Open reading frame ORF ORFs Pathogenesis pathogenicity recruit recruitment replicate replicated required respiratory tract Result resulting Sequencing serotype significant difference similarity supplementary material tissue Tissue distribution tonsils tracheal vaccinal Vaccines variant variants viral replication virus whole genome Whole genome sequencing [DOI] 10.1186/s12917-018-1720-9 PMC 바로가기 [Article Type] Research Article