Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR AssayResearch Article Published on 2018-11-062022-10-27 Journal: BioMed Research International [Category] Coronavirus, [키워드] cause clinical sample Conventional coronavirus described detect detection Detection limit development diarrhea evaluated family Coronaviridae Infection limit of detection much higher Newborn positive rate Rapid real-time RT-PCR required RNA molecule RT-PCR RT-PCR assay vomiting watery [DOI] 10.1155/2018/5035139 PMC 바로가기 [Article Type] Research Article
Porcine transmissible gastroenteritis virus nonstructural protein 2 contributes to inflammation via NF-κB activationResearch Paper Published on 2018-10-292022-10-28 Journal: Virulence [Category] Coronavirus, [키워드] activated activating acute enteritis Affect Amino acid amino acid residue bovine cause caused Cell contribute Critical Culture demonstrated domain down-regulation Eagle epithelial cell line exhibited expression fetal gastroenteritis indicated individual Infection infections Inflammation Inflammatory response inhibitor intestinal intestinal inflammation involved IκB monoclonal Necrosis NF-κB NF-κB NF-κB activation nitric oxide NSP2 nuclear p65 Pathogenesis pro-inflammatory cytokine pro-inflammatory cytokines Protein Regulation Replication reporter reverse transcription significantly suggested TGEV These data tissue transcription factor transmissible gastroenteritis virus Viral protein virus [DOI] 10.1080/21505594.2018.1536632 PMC 바로가기 [Article Type] Research Paper
MicroRNA-221-5p Inhibits Porcine Epidemic Diarrhea Virus Replication by Targeting Genomic Viral RNA and Activating the NF-κB PathwayArticle Published on 2018-10-292022-10-29 Journal: International Journal of Molecular Sciences [Category] Coronavirus, [키워드] 3’ UTR 3′ untranslated region activate activating biological processes cause Critical cytokine signaling demonstrated dose-dependent manner economic loss epidemic diarrhea etiological agent expression Gene Expression genomic RNA in viral Infection inhibit inhibited Innate immunity interferon investigated involved microRNA-221-5p miRNA miRNAs MX1 NF-κB NF-κB NF-κB pathway noncoding RNA nuclear nuclear translocation overexpression p65 pathway PEDV porcine epidemic diarrhea virus (PEDV) previous study Regulation Regulatory Replication reported RNA Signaling target targeting targets therapeutic strategy upregulating UTR viral genome viral replication virus [DOI] 10.3390/ijms19113381 PMC 바로가기 [Article Type] Article
White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in batsArticle Published on 2018-10-192022-10-29 Journal: Scientific Reports [Category] Coronavirus, [키워드] anti-viral response antibody average bat bats cause Contact coronavirus correlated cytokine disease severity down-regulate exhibited expression Increased inoculated intestine Level molecular response Myotis pathology pathway persistence Protein Replication severity syndrome systemic effect tested transcripts viral presence Viral RNA viral shedding virus [DOI] 10.1038/s41598-018-33975-x PMC 바로가기 [Article Type] Article
Human genetic variation in GLS2 is associated with development of complicated Staphylococcus aureus bacteremiaGLS2의 인간 유전적 변이는 복잡한 황색 포도구균 균혈증의 발병과 관련이 있습니다Research Article Published on 2018-10-052022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] activate age allele Analysis association Bacteremia Bacteria Bacterial Blood cells bloodstream Bonferroni correction candidate gene capture cause causes Cell coding variant complex Complication Control correlated CRISPR CRISPR-Cas9 cumulative cumulative effect death eight ENCODE evaluated examined exhibited Exome expression expression level Factor Frequency gene-based analysis gene-expression Genetic genetic factors genetic risk factor Genetic variant genetic variants Genetic variation Genome GLS2 Glutamine growth healthy controls Hospitalization Host Human Human genome immune response individual Infection infections Interaction key factor leukocyte leukocyte activation Logistic regression analysis Macrophage macrophage cells metabolism Microarray missense variant nitric oxide plasma concentration Protein regulate Regulatory Replication risk S . aureus sequence Sequencing Sex significantly significantly lower Staphylococcus aureus susceptible the strongest These cells Transcription variant variants Variation variations WES White blood cell White blood cells wild-type cells [DOI] 10.1371/journal.pgen.1007667 PMC 바로가기 [Article Type] Research Article
Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10Research Articles Published on 2018-09-212022-10-28 Journal: Bioscience Reports [Category] Coronavirus, [키워드] A549 cells addition airway epithelial cell antiviral genes antiviral response cause cells cellular processe CXCL10 dendritic cells determine Factor fibroblast cells Genes high mortality rate Host Human IL6 IL8 independent infected cell Infection interactions knowledge lung MERS-CoV Middle East Middle East respiratory syndrome Coronavirus monoclonal antibody monocyte-derived macrophage motif murine N-terminal domain nucleocapsid nucleocapsid protein overexpressed overexpression Patient PCR Protein regulate regulated reported residue Respiratory disease respiratory syndrome coronavirus secretion selected Severity of disease TNF transcriptional transcriptionally transiently transfected translational up-regulate up-regulated was used [DOI] 10.1042/BSR20181059 PMC 바로가기 [Article Type] Research Articles
Transmissible gastroenteritis virus infection decreases arginine uptake by downregulating CAT-1 expressionResearch Article Published on 2018-09-202022-10-28 Journal: Veterinary Research [Category] Coronavirus, MERS, [키워드] Activation amino acid transporter Analysis arginine cationic cause coronavirus decrease diarrhea downregulating Effect epithelial cell line evaluated expression growth factor hpi HPLC infect Infection intestinal intestinal epithelial cells investigated involved mechanism phospho-protein Post-infection receptor reduced target TGEV transmissible gastroenteritis virus [DOI] 10.1186/s13567-018-0591-1 PMC 바로가기 [Article Type] Research Article
Recombinant Infectious Bronchitis Viruses Expressing Chimeric Spike Glycoproteins Induce Partial Protective Immunity against Homologous Challenge despite Limited Replication In VivoVaccines and Antiviral Agents Published on 2018-09-122022-10-30 Journal: Journal of Virology [Category] Coronavirus, MERS, [키워드] avian infectious bronchitis virus BeauR bronchitis cause cellular challenge Characteristics characterized chimeric ciliary Clinical sign Clinical signs conducted conformational contagious coronavirus cross-protection determine expressing glycoprotein Heterologous homologous IBV IMPROVE in vitro in vivo induce Limited Live attenuated vaccines M41 mucosal tissue Partial partial protection PROTECT protective immunity recombinant recombinant vaccine reduction in reductions in replicated Replication Respiratory disease responsible rIBV S1 S1 and S2 S2 subunit serotype serum serum antibody single vaccination spike strain surface protein the S1 subunit tracheal Trial vaccination Vaccine Viral load viral RNA load virulent virus [DOI] 10.1128/JVI.01473-18 PMC 바로가기 [Article Type] Vaccines and Antiviral Agents
Crystal structure of the post‐fusion core of the Human coronavirus 229E spike protein at 1.86 Å resolutionResearch Papers Published on 2018-09-102022-10-27 Journal: Acta crystallographica. Section D [Category] Coronavirus, MERS, [키워드] addition Adults appear cause comparison Complete conserved coronavirus coronavirus 229E crystal structure electrostatic electrostatic interaction facilitate HCoV‐229E heptad repeat host cell host membranes Human human coronavirus hydrophobic hydrophobic interaction immune systems individual inhibit inhibitor Interaction involved MERS Mild Mortality opposite peptide polar post‐fusion core preserved Protein representing reveal S1 domain S2 domain SARS severe complication small molecule Spike protein structures subsequent surface potential upper respiratory infection virus was determined weakened X‐ray structure [DOI] 10.1107/S2059798318008318 PMC 바로가기 [Article Type] Research Papers
SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell deathArticle Published on 2018-09-052022-11-01 Journal: Cell Death & Disease [Category] Coronavirus, MERS, [키워드] activate addition Apoptotic cell death autophagy caspase-1 cause clinical manifestation contributing to dysfunction Effect Factor Genes helping implicated interact lysosomal lysosomal membrane membranes molecular mechanism necrotic cell death NLRP3 inflammasome assembly nucleus occur oligomerization Open reading frame pathophysiologic pathway Pathways Potassium Protein reading SARS-CoV SARS-CoV infection severe lung pathology target TFEB Transcription translocate Trigger while [DOI] 10.1038/s41419-018-0917-y PMC 바로가기 [Article Type] Article