Human Coronavirus NL63 Open Reading Frame 3 encodes a virion-incorporated N-glycosylated membrane proteinResearch Published on 2010-01-152022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] accessory protein addition Alpha Amino acid Analysis antiserum C-terminus Cell conserved coronavirus Coronaviruses cytosolic detected digestion ENCODE ERGIC Evidence expression Extracellular FLAG-tagged fluorescence microscopy Frame function Golgi Huh-7 cell Human human coronavirus immunofluorescence In-vitro Infected lysosomal membrane membrane protein N-terminus NL63 nucleocapsid Occurrence ORF pathogenic coronavirus predicted Protein purified reading Respiratory tract infections Result sequence shown structural protein subsequent Topology translation viral particle virion [DOI] 10.1186/1743-422X-7-6 PMC 바로가기 [Article Type] Research
Cleavage of the SARS Coronavirus Spike Glycoprotein by Airway Proteases Enhances Virus Entry into Human Bronchial Epithelial Cells In VitroResearch Article Published on 2009-11-172022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] 16HBE ACE2 ACE2 binding Activation acute respiratory syndrome against SARS-CoV airway Amino acid Analysis arginine residue bronchial bronchial epithelial cell cell entry Chromatography cleavage cleaved coronavirus demonstrated dependent on different Efficacy Efficiency exhibited expand expressing expression Extracellular finding full-length glycoprotein host cell HPLC Human implication in vitro investigated lack liquid mechanism molecular basis mutant not affect performance potential therapeutic agent protease Proteases Protein R667 R797 Receptor binding regulate Respiratory system S-protein SARS-CoV SARS-CoV infection Sequencing shown spike susceptibility susceptible Treatment trimer trypsin viral entry viral envelope virus virus entry [DOI] 10.1371/journal.pone.0007870 PMC 바로가기 [Article Type] Research Article