Catching COVID: Engineering Peptide-Modified Surface-Enhanced Raman Spectroscopy Sensors for SARS-CoV-2코로나 잡기: SARS-CoV-2용 펩티드 수정 표면 강화 라만 분광기 센서 엔지니어링Article Published on 2021-09-242022-09-11 Journal: ACS Sensors [Category] SARS, 변종, 치료기술, [키워드] Accuracy ACE2 albumin amplification angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 biomimetic biosensor bovine bovine serum albumin complex COVID-19 Engineering generate globe highlighting IMPROVE limit of detection peptide peptide-based peptides probe provide quantification quantitative assay Raman spectroscopy SARS-CoV-2 selective selectivity sensor SERS serum albumin specificity Spectroscopy Spike protein the spike protein unique variants Viral protein Viral proteins virus viruses [DOI] 10.1021/acssensors.1c01344 PMC 바로가기 [Article Type] Article
Peptides Derived From S and N Proteins of Severe Acute Respiratory Syndrome Coronavirus 2 Induce T Cell Responses: A Proof of Concept for T Cell VaccinesMicrobiology Published on 2021-09-242022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] acute respiratory syndrome administration alleviate CD8 concept coronavirus coronavirus 2 CTL cytotoxic T lymphocyte dose-dependent manner effective effector memory elicit epitope hamster highlight human ACE2 immunization in vivo increase indicated induce interferon-γ mice neutralizing antibody nucleocapsid protein peptide peptide vaccine Peptide vaccines peptides proof proportion Pulmonary pathology reduce respiratory response RNA SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike significantly statistically significant T cell T cell response T cell responses T cells target cell the SARS-CoV-2 transgenic mice tumor necrosis factor-α Vaccine variant viral titer virus [DOI] 10.3389/fmicb.2021.732450 PMC 바로가기 [Article Type] Microbiology
Epitope Profiling Reveals the Critical Antigenic Determinants in SARS-CoV-2 RBD-Based AntigenImmunology Published on 2021-09-212022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, [키워드] analyzed antibodies Antibody Response Antigen B-cell epitope binding caused characterized conserved COVID-19 COVID-19 pandemic cross-reactive antibody determinant diagnostic domain effort epitope facilitate globe humoral IFA immunization immunized immunodominant immunodominant epitope immunogenic in viral Infection information linear mapped mice monoclonal antibody neutralization activity Neutralizing antibodies peptide peptides Profiling Protein public health crisis RBD RBM receptor binding motif Result SARS-CoV SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 S trimer SARS-CoV-2 spike SARS-CoV-2 strains SARS-CoV-2 vaccine sera Spike protein Structural analysis the antibody response the RBD [DOI] 10.3389/fimmu.2021.707977 PMC 바로가기 [Article Type] Immunology
Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptidesSARS-CoV-2 펩타이드와의 복합체에서 HLA-A*02:01의 단백질 정제 및 결정화Article Published on 2021-09-172022-09-11 Journal: STAR protocols [Category] SARS, 치료법, [키워드] Antigen can be used Complete complex crystallization Crystallography expression HLA HLA class I HLA class I molecules Human Leukocyte Antigens immunology leukocyte nucleocapsid protein peptide peptides protein expression Protein expression and purification Protein purification protocol purification SARS-CoV-2 peptide T-cell Response T-cell responses the SARS-CoV-2 understanding viral peptide viral peptides X-ray X-ray crystallography [DOI] 10.1016/j.xpro.2021.100635 PMC 바로가기 [Article Type] Article
Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19광범위하게 지시된 SARS-CoV-2-특이적 CD4+ T 세포 반응에는 급성 및 소실된 COVID-19 환자의 막 및 핵단백질 내에서 자주 검출되는 펩티드 특이성이 포함됩니다.Research Article Published on 2021-09-162022-09-10 Journal: PLoS Pathogens [Category] SARS, 치료기술, 치료법, 치료제, [키워드] assays Author average binding affinity breadth CD4+ T cell Cohort cohort of patient COVID-19 cytokine data set defined dominant elicited ELISPOT ENCODE enzyme epitope Epitopes Ex vivo examined FIVE Frequency HLA HLA binding HLA class II ICS IFN-γ immune response immune responses immunological Immunopathogenesis in vitro include individual individual immune response interferon interferon-γ intracellular cytokine staining M protein membrane MHC class N protein novel nucleoprotein one patient overlapping Patient patients peptide peptides phenotype Protein resolved response response frequency SARS-CoV-2 envelope SARS-CoV-2 genome SARS-COV-2 infection screened Specificities specificity Spike protein structural protein structural proteins subsequent T cell T cell epitope T cell response T cell responses T cells tetramer the N protein the SARS-CoV-2 the spike protein to define Vaccine design Viral Viral proteins vitro cultivation [DOI] 10.1371/journal.ppat.1009842 PMC 바로가기 [Article Type] Research Article
ImmunosuppressiveTherapies Differently Modulate Humoral- and T-Cell-Specific Responses to COVID-19 mRNA Vaccine in Rheumatoid Arthritis PatientsImmunology Published on 2021-09-142022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, SARS, [키워드] Administered adverse event Anti-RBD antibodies Antibody Response biological therapy BNT162b2 vaccine CD4 CD8 Cell cell-mediated response clinical examination clinical impact college correlation COVID-19 dependent on Disease activity DMARD (disease modifying anti-rheumatic drug) enrolled evaluated FACS analysis HCW HCWs Health care identify IFN-γ immune response immunosuppressive immunosuppressive therapies Immunosuppressive treatment indications Local magnitude majority mRNA mRNA vaccine objective Patient patients peptides positive reduced regimen Registered remained response responses against Result Rheumatoid rheumatoid arthritis Safe scored significant increase significantly significantly lower spike-specific T-cell response T cell response T cells T-cell Response the interferon the vaccine therapeutic therapy treated vaccination vaccine administration was performed Whole blood [DOI] 10.3389/fimmu.2021.740249 PMC 바로가기 [Article Type] Immunology
Profiling CD8 + T cell epitopes of COVID-19 convalescents reveals reduced cellular immune responses to SARS-CoV-2 variantsCOVID-19 회복기의 CD8 + T 세포 에피토프를 프로파일링하면 SARS-CoV-2 변이체에 대한 감소된 세포 면역 반응이 나타납니다Article Published on 2021-09-142022-09-11 Journal: Cell Reports [Category] SARS, 변종, [키워드] addition alleles binding CD8 CD8+ CD8+ T cells Cellular immune response cellular immunity circulating variants complex conserved contribute convalescent COVID-19 COVID-19 patient COVID-19 patients crystal structure delta variant determine disease severity epitope epitope peptide Epitopes HLA HLA-A human coronaviruses identify Immunity K417 mutated Mutation new SARS-CoV-2 patients peptides Profiling reduce reduced reveal SARS-CoV-2 SARS-CoV-2 epitope SARS-CoV-2 epitopes SARS-CoV-2 variant SARS-CoV-2 variants severity of COVID-19 significantly Spike protein T cell T cell epitope T cell epitopes T cell response T cell responses T cells the disease the spike protein Transmissibility variants [DOI] 10.1016/j.celrep.2021.109708 PMC 바로가기 [Article Type] Article
Potential Therapeutic Effect of Micrornas in Extracellular Vesicles from Mesenchymal Stem Cells against SARS-CoV-2SARS-CoV-2에 대한 중간엽 줄기세포의 세포외 소포에서 Micrornas의 잠재적 치료 효과Article Published on 2021-09-122022-09-11 Journal: Cells [Category] SARS, 변종, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Alter anti-SARS-CoV-2 anti-viral response binding cellular physiology complementary components conserved coronavirus COVID-19 Cytokine storm Cytopathic effect cytopathic effects differentiation Effect EVs expressed expression extracellular vesicle Extracellular vesicles FIVE human gene human genes immune response immune responses infected cell infected cells Infection inhibiting membrane-bound mesenchymal stem cell micro-RNA microenvironment miR-181a-5p miR-23a-3p miRNA miRNAs mRNAs mutated myriad nucleic acids peptides Potential Proinflammatory response proliferation Proteins Region regulate released rescue respiratory RNAs SARS-CoV-2 SARS-CoV-2 virus sequence severe acute respiratory syndrome Coronavirus STEM suppression tested therapeutic agent therapeutic agents UTR variant variety Vesicle vesicles Viral viral gene viral genome viral infection viral replication [DOI] 10.3390/cells10092393 PMC 바로가기 [Article Type] Article
Recognition of Divergent Viral Substrates by the SARS-CoV-2 Main ProteaseSARS-CoV-2 주요 프로테아제에 의한 다양한 바이러스 기질의 인식Article Published on 2021-09-102022-09-11 Journal: ACS Infectious Diseases [Category] Coronavirus, SARS, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Amino acid amino acid substitution amino acid substitutions antiviral drug catalysis catalytic changes in cleavage cleavage site cleave conserved coronavirus coronavirus disease Coronaviruses COVID-19 crystal structure crystal structures Efficiency ENhance homologous junction lifecycle M pro MPro NSP8 parameters peptide peptides polyprotein protease Proteases provide Recognition recognize Replication residue SARS-CoV-2 severe acute respiratory syndrome Coronavirus Structure structures substrate the SARS-CoV-2 unique viral nonstructural protein viral nonstructural proteins viral replication Virology Visualization [DOI] 10.1021/acsinfecdis.1c00237 PMC 바로가기 [Article Type] Article
Accelerating antibiotic discovery through artificial intelligenceReview Article Published on 2021-09-092022-10-04 Journal: Communications Biology [Category] 신약개발, [키워드] antibiotic Antibiotics Antimicrobials Computational drug computational method computer-aided drug design de novo dimension Drug discovery drug-likeness drug-resistant Efficacy evolutionary arms race form inquiry invasive machine learning molecular offer Organisms pathogen peptides proliferation replaced reproducibility Research Stride therapy traits urgency [DOI] 10.1038/s42003-021-02586-0 PMC 바로가기 [Article Type] Review Article