Limitations of using feline coronavirus spike protein gene mutations to diagnose feline infectious peritonitisResearch Article Published on 2017-10-052022-10-28 Journal: Veterinary Research [Category] Coronavirus, [키워드] Analysis Antigen coronavirus coronavirus spike protein determine diagnose Diagnosis faece Faeces fatal disease Feline infectious peritonitis gene mutation healthy histopathological identification immunohistochemistry implicated IMPROVE Infection limitation mutated Mutation positive Post-mortem Primers purified RNA RT-qPCR S gene Sample sensitivity Sequencing specificity Spread supplementary material tissue tissue sample viral spike was performed [DOI] 10.1186/s13567-017-0467-9 PMC 바로가기 [Article Type] Research Article
Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte culturesArticle Published on 2016-01-292022-10-29 Journal: Scientific Reports [Category] Coronavirus, MERS, [키워드] abnormality absence affected antibody blood cell CD8 Clinical sign Clinical signs coronavirus Coronavirus infection Culture Feline infectious peritonitis Hypothesis Immune cell immunological infect Infection Infectious virus intestinal investigated less leukocyte Mild mutant mutated Mutation mutations neutralising antibody not different occurred raise regulatory T cells Replication reveal SPF these cell Viraemia viral genome Virological virus virus [DOI] 10.1038/srep20022 PMC 바로가기 [Article Type] Article
Identification of the Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoV for the Design of a Genetically Stable VaccineResearch Article Published on 2015-10-292022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] addition against SARS-CoV analyzed biosafety carboxy-terminal region causing Cell culture chimeric contributed coronavirus Coronaviruses CoVs deleted Deletion duplication E gene E protein effective epidemic diarrhea full-length Genetic Host identification immune response in vitro in vivo indicated induce insertion interferon response introduced Live attenuated vaccine manuscript mechanism membrane MERS-CoV mice Middle East molecular mechanism morbidity and mortality motif mutant virus mutated Mutation not affect parental passage pathogenicity PEDV phenotype Protective Protein provided recombinant remained restored resulting reversion Safe SARS-CoV SARS-CoV vaccine sequence stability STABLE syndrome the vaccine therapy transmembrane protein understanding Vaccine vaccine candidate Vaccines variant virulence virulent virus virus virus attenuation virus genome [DOI] 10.1371/journal.ppat.1005215 PMC 바로가기 [Article Type] Research Article
PRO40 Is a Scaffold Protein of the Cell Wall Integrity Pathway, Linking the MAP Kinase Module to the Upstream Activator Protein Kinase CPRO40은 MAP 키나제 모듈을 업스트림 활성제 단백질 키나제 C에 연결하는 세포벽 무결성 경로의 스캐폴드 단백질입니다.Research Article Published on 2014-09-042022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] activator adapter adapters Affinity purification Alzheimer's disease approach ascomycete asexual development bind Cancer Cell cell differentiation Cell fusion cell wall characterized combating conserved Control deletion mutant deletion mutants determinant developmental developmental processe developmental processes disease domain ENCODE environmental cue environmental cues eukaryote eukaryotes eukaryotic Evidence experiment Extensive filamentous fungi fruiting bodies fungal fungi fungus Genetic growth homolog implicated integrity Interaction interaction partner involved kinase MAPK mass spectrometry mitogen mitogen-activated protein kinase module molecular MOST mutant mutated N-terminal Pathogenesis pathogenicity pathway Pathways Phosphorylation polar Protein protein kinase protein kinase C Regulation required scaffold protein Signal transduction Signaling Sordaria macrospora Stress stress response symbiosis the cell upstream vegetative growth wall yeast [DOI] 10.1371/journal.pgen.1004582 PMC 바로가기 [Article Type] Research Article
Structural Basis for the Ubiquitin-Linkage Specificity and deISGylating Activity of SARS-CoV Papain-Like ProteaseResearch Article Published on 2014-05-222022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] 3D structure abrogated activate activated activity acute respiratory syndrome amino acid residue B-cell Basis binding cleaving complex coronavirus coronavirus distal domain DUB ENCODE enzyme function Genome help higher affinity highlight Host host cell hydrolysis identify in viral inhibited innate immune response interact Interaction interferon involved ISG15 mechanism MERS Middle East molecular mutant mutated NFκB nuclear Papain-like protease Pathogenesis PLPro polyprotein post-translational modification Protease activity Protein Proteins provide reduced required residue residues respiratory reveal SARS virus SARS-CoV short circuit signaling molecule signaling pathway significantly similar study specificity stimulated substrate substrates thumb unique viral replication virus was determined X-ray [DOI] 10.1371/journal.ppat.1004113 PMC 바로가기 [Article Type] Research Article
A HECT Ubiquitin-Protein Ligase as a Novel Candidate Gene for Altered Quinine and Quinidine Responses in Plasmodium falciparum플라스모듐 팔시파룸(Plasmodium falciparum)에서 변경된 퀴닌 및 퀴니딘 반응에 대한 새로운 후보 유전자로서의 HECT 유비퀴틴-단백질 리가제Research Article Published on 2014-05-152022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Altered analysis antimalarial antimalarial drug Asia association candidate candidate gene changes in chromosome chromosome 13 complex conducted contribute contributing to cross cytotoxic activity defend demonstrated drug Efficacy experiment Factor Factors Genetic genetic screens genetic trait identify IMPROVE initial isolate Laboratory Ligase likelihood locus malaria malaria parasite moderate multiple allele multiple alleles mutated novel occur P. falciparum Plasmodium falciparum preservation progeny Quantitative quantitative trait locus quinidine Quinine response responsiveness segment sensitivity strain subset Surveillance susceptibility These data Treatment treatment failure ubiquitin ligase [DOI] 10.1371/journal.pgen.1004382 PMC 바로가기 [Article Type] Research Article
CNNM2 Mutations Cause Impaired Brain Development and Seizures in Patients with HypomagnesemiaCNNM2 돌연변이는 저마그네슘혈증 환자의 뇌 발달 장애 및 발작을 유발합니다Research Article Published on 2014-04-032022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] accompanied Affect Brain brain development brain malformation brain malformations cation channel cause caused Cell cellular clinical variability CNNM2 Combination cRNA cyclin M2 de novo de novo heterozygous missense mutations demonstrated development Diagnosis disease Effect embryonic explain expressing FIVE gene encoding Genetic genetic cause genetic component genetic disease genetic etiology genetic heterogeneity handling HEK293 cell HEK293 cells heterozygous homeostasis human disease hypomagnesemia identify Impaired IMPROVE in vitro activity increase inheritance injection intellectual disability isoform kidney knockdown loss-of-function Magnesium mammalian Mental mental disability mental retardation Missense mutation mutant mutated Mutation neurodevelopmental impairment neurodevelopmental impairments neurological Neurological diseases occur Pathomechanism Pathomechanisms Patient patients with moderate phenotype Phenotypes physiological Physiology Protein reduced Regulation renal rescued Seizure Seizures shown the disease therapeutic drug Treatment TRPM7 unique wild-type zebrafish [DOI] 10.1371/journal.pgen.1004267 PMC 바로가기 [Article Type] Research Article
Residue analysis of a CTL epitope of SARS-CoV spike protein by IFN-gamma production and bioinformatics predictionResearch Article Published on 2012-09-102022-10-28 Journal: BMC Immunology [Category] Coronavirus, Fulltext, MERS, [키워드] acute respiratory syndrome Alanine Analysis ANN antigenic epitopes ARB binding bioinformatics caused cells CTL CTL epitope demonstrated DNA electrostatic ELISA epitope exaggerate FACS functional hydrophobic IFN-gamma immunized mice induce Infectious disease Interaction lysine mutant mutated Novel coronavirus peptide peptides predicted replaced reported residue Result S protein SARS CoV SARS-CoV SARS-CoV S SARS-CoV spike protein severe Spike protein splenocyte steric T cell epitope TCR the binding affinity the mean was used [DOI] 10.1186/1471-2172-13-50 PMC 바로가기 [Article Type] Research Article
Prevention of Cytotoxic T Cell Escape Using a Heteroclitic Subdominant Viral T Cell DeterminantResearch Article Published on 2008-10-242022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, MERS, [키워드] alteration amino acid substitution analysis antigen-specific T cell association Author CD8+ T cell changes in clinical disease complex Complex molecule conformation Consensus Critical CTL cytotoxic determinant Disease progression Diseases elicit Enhancing epitope escape expressed functional Hepatitis histocompatibility HIV immune response immune responses immunodominant Infected Local Major memory MHC class I mice molecular basis motif mouse hepatitis mutated Mutation natural infection occur pathogen peptide performed prevention PROTECT Protective recombinant residue response significantly higher stability Subdominant T cell T cell epitope T cell response tumor immunity Vaccine development viral infection virus virus wildtype [DOI] 10.1371/journal.ppat.1000186 PMC 바로가기 [Article Type] Research Article
The SARS Coronavirus S Glycoprotein Receptor Binding Domain: Fine Mapping and Functional CharacterizationResearch Published on 2005-08-252022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] ACE2 Alanine amino acid residue appear binding binding energy Cell characterization characterized cloned contribute decrease determined eight expressed expression functional Glutamine glycoprotein glycosylation glycosylation site identify immune responses include indicate initiated kDa lack Li mapping mechanism molecular weight Mutagenesis mutated Mutation not affect R426 RBD receptor receptor ACE2 reduced required residue residues S RBD SARS Coronavirus significantly Simultaneous submitted suggested the RBD the receptor-binding domain These data [DOI] 10.1186/1743-422X-2-73 PMC 바로가기 [Article Type] Research