Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics
디지털 PCR은 SARS-CoV-2 진단을 위한 RT-qPCR Cq 값의 해석을 보강할 수 있습니다.
Article
Published on
Journal: Methods (San Diego, Calif.) [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, 진단,
Journal: Methods (San Diego, Calif.) [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, 진단,
[키워드] acute health crisis
acute respiratory disease
Analysis
bile salt
bile salts
caused
common knowledge
Compound
coronavirus
coronavirus disease
Coronavirus disease 2019
coronavirus SARS-CoV-2
COVID-19
Cq value
Cq value validity
Cq values
cut-off value
cycle threshold
demonstrated
diagnostics
Digital
digital PCR
disagreement
disease
dPCR
Effect
Efficiency
effort
exacerbated
Health
Health crisis
highlight
in urine
include
individual
individuals
infectious
inhibiting
inhibitor
inhibitors
Interpretation
investigated
knowledge
MIQE and dMIQE guidelines
molecular
parameters
PCR
performed
positive result
qPCR
quantification
quantitative PCR
Reporting
Respiratory disease
reverse transcription
Reverse transcription quantitative PCR
RNA extraction
RT-qPCR
Saliva
Sample
Sample matrix effects
SARS-CoV-2
separated
shown
Standard
Standard material
susceptible
Swab
test results
the disease
These data
Transmission
Urine
Validity
Variability
variable
Viral
viral burden
Viral RNA
Virus quantification
[DOI] 10.1016/j.ymeth.2021.08.006 PMC 바로가기 [Article Type] Article
[DOI] 10.1016/j.ymeth.2021.08.006 PMC 바로가기 [Article Type] Article