Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in ChinaResearch Paper Published on 2016-04-152022-10-28 Journal: Science China. Life sciences [Category] Coronavirus, [키워드] Analysis antigenic antigenicity Antisera Beijing canine Canine coronavirus canine respiratory coronavirus China cloned conserved coronavirus Coronavirus infection cross-react cross-reactivity E. coli Escherichia coli expressed expression Final group HCoV-229E HKU1 human coronaviruses indicated mice N gene NL63 nucleocapsid protein OC43 polyacrylamide gel purified raised Respiratory Coronavirus SDS-PAGE sequenced size exclusion chromatography sodium dodecyl sulfate Swab virus were expressed [DOI] 10.1007/s11427-016-5038-1 PMC 바로가기 [Article Type] Research Paper
Molecular Characterization and Phylogenetic Analysis of Porcine Epidemic Diarrhea Viruses Associated with Outbreaks of Severe Diarrhea in Piglets in Jiangxi, China 2013Research Article Published on 2015-03-192022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] accumulated Amino acid amino acid substitution Analysis analyzed antigenic caused change Characteristics characterization characterized China Chinese Complete contagious death diarrhea Effectiveness epidemic diarrhea epitope Genetic Genetic variation Genome genome sequence hydrophilicity identify identity less Multiple Mutation N gene Neutralizing nucleotide ORF1a/b ORF3 outbreak PEDV proximal ranged S gene strain Strains structural protein unique Vaccine viral disease virus viruses vomiting watery [DOI] 10.1371/journal.pone.0120310 PMC 바로가기 [Article Type] Research Article
A structural view of coronavirus–receptor interactionsArticle Published on 2014-10-142022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] antibody antigenic binding Cell cell entry circulation complex structure complex structures coronavirus CoV crystal structure determinant Ectoenzymes Evolution facilitate Features glycoprotein Glycoproteins highlight host cell host cells hosts infections Interaction membrane multifunctional neutralization neutralize outbreak Particle penetrate penetration platform RBD receptor Receptor-binding domain receptors recognize reported responsible specificity surface receptor Transmission virus virus entry Virus neutralization Virus–receptor [DOI] 10.1016/j.virusres.2014.10.005 PMC 바로가기 [Article Type] Article
Substitution at Aspartic Acid 1128 in the SARS Coronavirus Spike Glycoprotein Mediates Escape from a S2 Domain-Targeting Neutralizing Monoclonal AntibodyResearch Article Published on 2014-07-142022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] acid addition Alanine Amino acid antigenic bind binding Cell epitope escape etiological agent exhibited expression humans in vitro infect Infectious disease Interaction Lip mAb mAbs monoclonal antibody murine mutant Mutation neutralization Neutralizing neutralizing antibody no effect overcome Particles Prevent Protein pseudotyped residue respiratory S protein S2 subunit SARS-CoV SARS-CoV infection SARS-like CoV shown significant decrease SL-CoVs spike Substitution surface Spike the S protein viral entry virion virus wild-type wild-type virus zoonotic [DOI] 10.1371/journal.pone.0102415 PMC 바로가기 [Article Type] Research Article
Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virusResearch Published on 2012-10-012022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] Amino acid Analysis Antigen antigenic B-cell epitope C-terminus cell line conserved Coronaviruses demonstrated diagnostic different elicit ELISA enzyme-linked immunosorbent epidemic diarrhea epitope epitope-based vaccines fusion protein gastroenteritis GST identification information linear M protein membrane monoclonal antibody motif overlapping PEDV protective antibodies Protein provide reactivity Result secreting sequence serum shown Strains virus was obtained were expressed western blot [DOI] 10.1186/1743-422X-9-225 PMC 바로가기 [Article Type] Research
Structural Bases of Coronavirus Attachment to Host Aminopeptidase N and Its Inhibition by Neutralizing AntibodiesResearch Article Published on 2012-08-022022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] acute respiratory syndrome aminopeptidase aminopeptidase N antibody antigenic Antiviral APN attachment Author base binding blocked Cancer Cancer therapy Cell cell entry cell entry receptor cell membrane colds complex coronavirus CoV CoVs crystal structure determinant determine dimeric drugs ectodomain engage enteric diseases facilitate gastroenteritis Host host cells Human immune immune response in some Infection infections Infectious disease information inhibition Interaction membrane-bound neutralization Neutralizing antibodies neutralizing antibody Prevent Protein RBDs receptor Receptor binding receptor binding motif related reported residue respiratory diseases shown specificity spike glycoprotein Strains subsequent subset TGEV the RBD the receptor-binding domain the S protein unique Vaccines virus virus virus particle viruses [DOI] 10.1371/journal.ppat.1002859 PMC 바로가기 [Article Type] Research Article
Regulatory T Cells in Arterivirus and Coronavirus Infections: Do They Protect Against Disease or Enhance it?Review Published on 2012-05-152022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] Activation adaptive Against antigenic arterivirus, porcine reproductive and respiratory syndrome virus CD25 CD28 CD4 CD8 Cell cells chronic inflammation Coronaviral coronavirus cytokine described develop disease Express expression form hallmark homeostasis host response IL-10 Immune homeostasis immune response immune tolerance induction Infection interleukin 2 lactate dehydrogenase lactate dehydrogenase-elevating virus maintain mean mouse hepatitis virus natural Other Pathogenesis PROTECT receptor Regulatory Regulatory T cell reproductive responsible subset syndrome T cell T cell subset T helper TGF-β the disease transcription factor Type viral persistence virus [DOI] 10.3390/v4050833 PMC 바로가기 [Article Type] Review
Antigenic characterization of severe acute respiratory syndrome-coronavirus nucleocapsid protein expressed in insect cells: The effect of phosphorylation on immunoreactivity and specificityArticle Published on 2007-05-112022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Analysis Antigen antigenic antigenicity assay baculovirus coronavirus correlation cross-reactivity determine diagnostic E. coli expressed facilitate immunoreactivity insect cell insect cells involved mAb molecular MOST N protein nucleocapsid nucleocapsid protein pathology phosphorylated Phosphorylation produced Protein protein phosphatase 1 reduced Research SARS infection SARS-CoV SARS-CoV N SARS-CoV N protein serum Severe acute respiratory syndrome specificity treated were expressed [DOI] 10.1016/j.virusres.2007.03.019 PMC 바로가기 [Article Type] Article
Mutational dynamics of the SARS coronavirus in cell culture and human populations isolated in 2003Research Article Published on 2004-09-062022-10-28 Journal: BMC Infectious Diseases [Category] Coronavirus, [키워드] 2003 3CLpro adjusted Analysis antigenic antigenic protein appear arising calculated Cell culture China Co-infection Complete Contact Culture cultured isolate Deletion different estimate FIVE found functional Gene Genes Genetic Genome greater group growth hotel human population human populations human tissue human-to-human transmission in vitro in vivo isolate isolates molecular Mutation mutation rate Non-synonymous mutation North nucleotide substitution ORF ORFs passage pathogen Patient per day polymerase Proteins related respiratory Result RNA virus SARS Coronavirus SARS-CoV selection pressure sequence sequenced severe Singapore Taiwan the epidemic the SARS epidemic tissue sample Transmission transmission patterns undergo Vero Cell viral genome was used whole genome sequence [DOI] 10.1186/1471-2334-4-32 PMC 바로가기 [Article Type] Research Article
Molecular analysis of the S1 subunit of the spike glycoprotein of respiratory and enteric bovine coronavirus isolatesArticle Published on 2002-01-302022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Allelic variation alteration Amino acid amino acid change amino acid substitution Analysis analyzed antigenic Bovine coronavirus Characteristics classical cleavage site Clustering compared diarrhea different Genetic group hydrophobicity isolate isolates led to nucleotide Phylogenetic analysis polymorphism predicted Probability Protein residue Respiratory and enteric BoCV S glycoprotein S1 subunit secondary structure sequence Sequencing significant changes in spike glycoprotein strain Strains the S1 subunit two subgroups variations [DOI] 10.1016/S0168-1702(02)00004-7 PMC 바로가기 [Article Type] Article