CXCR2 Signaling Protects Oligodendrocytes and Restricts Demyelination in a Mouse Model of Viral-Induced DemyelinationResearch Article Published on 2010-06-282022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] activating affected Apoptosis apoptotic cell BCL-2 binding caspase 3 Cell chemokine chronic infection cleavage cleaved clinical recovery CNS Culture CXCL1 CXCL2 CXCR2 decrease Demyelination disease elevated expression finding functional highlight increase in independent Infected Infection Inflammatory initiated inoculation Ligand Lineage mice Model modulation mouse mouse hepatitis virus Multiple Neuroinflammation neutralization neutralizing antiserum Oligodendrocyte pathology receptor sclerosis severity Signaling significantly susceptible sustained transcript viral infection [DOI] 10.1371/journal.pone.0011340 PMC 바로가기 [Article Type] Research Article
A randomized, double-blind, placebo-controlled study of an RNAi-based therapy directed against respiratory syncytial virusClinical Trial Published on 2010-05-112022-10-06 Journal: Proceedings of the National Academy of Sciences of [Category] 진단, [키워드] acquisition Administered Adult Adults Analysis antibody antiviral activity Antiviral effect assays clinical evidence concentrations decrease different double-blind Effectiveness enrolled exhibiting Factors healthy subject Human increase in independent Infected infected children Infection intranasal mechanism mRNA multiple logistic regression murine model nasal nasal washe nucleocapsid number of infected PCR Placebo placebo-controlled study placebo-controlled trial Proinflammatory cytokine proportion Protein protein expression Randomized recipient recipients representing respiratory syncytial virus RNA RSV RSV infection safety profile saline placebo significantly lower siRNA subjects tested therapeutic Therapeutic approach therapy treat uninfected unique viral culture was measured wild-type [DOI] 10.1073/pnas.0912186107 PMC 바로가기 [Article Type] Clinical Trial
Infidelity of SARS-CoV Nsp14-Exonuclease Mutant Virus Replication Is Revealed by Complete Genome SequencingResearch Article Published on 2010-05-062022-10-29 Journal: PLoS Pathogens [Category] Coronavirus, [키워드] accumulate acute respiratory syndrome Author bioinformatics clone compared competent Complete complex consensus genome coronavirus coronavirus exoribonuclease Coronaviruses Critical Culture decrease deep sequencing demonstrated Effect Evolution examined experiment Frequency full-length genome Genetic Genome genome replication genome sequence growth Impact in vitro inactivation increase in Infidelity lack less limit mechanism MOST murine hepatitis virus mutant mutant virus Mutation nonstructural protein nonstructural protein 14 nsp14 nsp14-ExoN Nsps passage Pathogenesis powerful tool quasispecy recognize Replication replication fidelity required resulting Revealed RNA genome RNA genomes RNA virus SARS-coronavirus SARS-CoV sequence stability the Coronaviridae to define total population unique viral growth viral replication viral RNA genome viral system virus virus wild-type SARS-CoV [DOI] 10.1371/journal.ppat.1000896 PMC 바로가기 [Article Type] Research Article
Broadly targeted multiprobe QPCR for detection of coronaviruses: Coronavirus is common among mallard ducks ( Anas platyrhynchos )Article Published on 2009-05-042022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] Ct, threshold cycle amplified Ana avian coronavirus competing conserved Coronaviruses CoV CoVs decrease detect detection duck fatal disease fecal samples found Frame FRET, fluorescence resonance energy transfer groups host range Human LNA, locked nucleic acid NQPCR, nuclease-based real-time reverse transcription PCR Nuclease-based probes ORF, open reading frame PCR, polymerase chain reaction Primers probe probes provide QPCR, quantitative real-time reverse transcription PCR Real-time PCR reduced reservoirs respiratory sample reverse transcription PCR RNA, Ribonucleic acid RT-PCR, reverse transcriptase polymerase chain reaction sensitivity sequence Split probe strategy Spread Strains TaqMan targets TD, touch down temperature protocol TET, tetrachloro-6-carboxyfluorescein Transmission variant viral evolution viral nucleic acid [DOI] 10.1016/j.jviromet.2009.04.022 PMC 바로가기 [Article Type] Article
The SARS Coronavirus S Glycoprotein Receptor Binding Domain: Fine Mapping and Functional CharacterizationResearch Published on 2005-08-252022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] ACE2 Alanine amino acid residue appear binding binding energy Cell characterization characterized cloned contribute decrease determined eight expressed expression functional Glutamine glycoprotein glycosylation glycosylation site identify immune responses include indicate initiated kDa lack Li mapping mechanism molecular weight Mutagenesis mutated Mutation not affect R426 RBD receptor receptor ACE2 reduced required residue residues S RBD SARS Coronavirus significantly Simultaneous submitted suggested the RBD the receptor-binding domain These data [DOI] 10.1186/1743-422X-2-73 PMC 바로가기 [Article Type] Research
The Severe Acute Respiratory Syndrome (SARS)-coronavirus 3a protein may function as a modulator of the trafficking properties of the spike proteinHypothesis Published on 2005-02-102022-10-28 Journal: Virology Journal [Category] Coronavirus, [키워드] addition biochemically Cell Coronaviruses cytoplasmic cytoplasmic tail decrease different domain Effect examined expressed expression genetics Genome homologue Hypothesis Impact in vitro in vivo infected cell Infection Interaction mechanism methodology modulate motif Open reading frame other coronaviruses pathway presentation Protein Replication reported respiratory resulting RNA SARS-CoV severe shown significantly Spike protein Testing the spike protein undergo unique viral infection viral pathogenesis Viral protein viral spread [DOI] 10.1186/1743-422X-2-5 PMC 바로가기 [Article Type] Hypothesis