PEDV Infection Generates Conformation-Specific Antibodies That Can Be Effectively Detected by a Cell-Based ELISAArticle Published on 2021-02-152022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] Analysis analyzed antibodies Antigen antiserum baculovirus Can cause cell-based ELISA contagious coronavirus disease ELISA enzyme-linked immunosorbent Epidemic epidemic diarrhea exhibited expressing full-length spike protein generate Genotype indicated Infection insect cell membrane protein PEDV produced sensitivity SEROLOGICAL STUDY serum serum samples specificity spike Spike-specific antibody staining trimeric spike viral disease virulent virus [DOI] 10.3390/v13020303 PMC 바로가기 [Article Type] Article
The rapid diagnosis and effective inhibition of coronavirus using spike antibody attached gold nanoparticles †Chemistry Published on 2021-01-182022-10-31 Journal: Nanoscale Advances [Category] COVID-19, [키워드] ACE2 acute respiratory syndrome aggregates aggregation angiotensin-converting enzyme 2 anti-spike antibody antibody Antigen baculovirus binding cell receptors Concentration coronavirus coronavirus disease COVID-19 death detect Diagnosis effective Express Gold nanoparticle HEK293T cells human cells Infection inhibit inhibiting Interaction interactions membrane observation probe pseudotyped Raman spectroscopy rapid diagnostic assay reported reporter responsible SARS-CoV-2 SARS-CoV-2 spike protein Spread undergo Vaccine Viral antigen virus virus infection virus particle virus particles [DOI] 10.1039/d0na01007c PMC 바로가기 [Article Type] Chemistry
Simple and rapid plaque assay for recombinant baculoviruses expressing influenza hemagglutininArticle Published on 2021-01-012023-06-23 Journal: Science progress [Category] 신종인플루엔자, [키워드] baculovirus hemagglutinin immunostaining Influenza virus plaque assay. [DOI] 10.1177/00368504211004261
A Novel Purification Procedure for Active Recombinant Human DPP4 and the Inability of DPP4 to Bind SARS-CoV-2Article Published on 2020-11-182022-10-30 Journal: Molecules [Category] COVID-19, [키워드] addition Alter Ammonium baculovirus bind binding binding affinity Cancer COVID-19 Culture Dipeptidyl peptidase 4 Diseases domain DPP4 ELISA full-length spike protein Human human cell human DPP4 hydrophobic Inflammatory insect cell insect cells insulin resistance Interaction MERS MERS virus novel pharmacological target posttranslational modification procedure produced protease Protein protocol purification question RBD recombinant Recombinant protein SARS-CoV-2 SARS-CoV-2 spike protein Spike protein steatosis surface receptor the SARS-CoV-2 the spike protein therapy type 2 diabete understanding virus were measured [DOI] 10.3390/molecules25225392 PMC 바로가기 [Article Type] Article
Parenterally Administered Porcine Epidemic Diarrhea Virus-Like Particle-Based Vaccine Formulated with CCL25/28 Chemokines Induces Systemic and Mucosal Immune Protectivity in PigsArticle Published on 2020-10-022022-10-28 Journal: Viruses [Category] Coronavirus, MERS, [키워드] adjuvant baculovirus CCL25 CCL28 cellular immunity chemokines Clinical sign control group Critical effective Efficacy epidemic diarrhea evaluated expressed expression vector fecal Generation group IgA IgG immune protection immune response Immunity immunized intramuscularly membrane modulate mucosal PEDV pig platform Porcine epidemic diarrhea virus Protective reduced Safe structural protein systemic Vaccine vaccine candidate Vaccines Viral diseases viral shedding virus Virus-like particle VLP VLPs while [DOI] 10.3390/v12101122 PMC 바로가기 [Article Type] Article
Mosquito Cell-Derived Japanese Encephalitis Virus-Like Particles Induce Specific Humoral and Cellular Immune Responses in MiceArticle Published on 2020-03-192023-08-29 Journal: Viruses [Category] E형 간염, [키워드] baculovirus Japanese encephalitis virus Mosquito virus-like particles [DOI] 10.3390/v12030336 PMC 바로가기 [Article Type] Article
Conserved HA-peptides expressed along with flagellin in Trichoplusia ni larvae protects chicken against intranasal H7N1 HPAIV challengeArticle Published on 2020-01-162023-06-22 Journal: Vaccine [Category] 조류인플루엔자, [키워드] Avian Influenza baculovirus Conserved peptides cross-protection flagellin H7 subtype influenza vaccine Pandemic flu. [DOI] 10.1016/j.vaccine.2019.11.006
Expression and purification optimization of an N-terminal Pfs230 transmission-blocking vaccine candidateN-말단 Pfs230 전파 차단 백신 후보의 발현 및 정제 최적화Article Published on 2019-08-012024-08-26 Journal: Protein Expression and Purification [Category] 말라리아, [키워드] baculovirus malaria P. falciparum Pfs230 Sf9 Vaccine [DOI] 10.1016/j.pep.2019.04.001 PMC 바로가기 [Article Type] Article
Rapid Construction and Immunogenicity Testing of a Novel H5 Virus-Like Particle Prototype Vaccine Against Clade 2.3.4.4 H5N8 Highly Pathogenic Avian Influenza VirusArticle Published on 2019-03-012023-06-02 Journal: Avian diseases [Category] 조류인플루엔자, [키워드] Avian Influenza baculovirus duck H5N8 immunogenicity recombinant vaccine Virus-like particle [DOI] 10.1637/11888-042718-Reg.1
Novel coronavirus-like particles targeting cells lining the respiratory tractResearch Article Published on 2018-09-052022-10-28 Journal: PLoS ONE [Category] Coronavirus, [키워드] ACE2 protein baculovirus Cell ciliated cell concerning displaying evaluated expressed expressing expression HCoV-NL63 Major novel Particle platform produced Production purification purified Research respiratory tract structural protein tissue tropism vaccine candidates Viral vector VLP VLPs [DOI] 10.1371/journal.pone.0203489 PMC 바로가기 [Article Type] Research Article