Utilizing the VirIdAl Pipeline to Search for Viruses in the Metagenomic Data of Bat SamplesArticle Published on 2021-10-062022-10-28 Journal: Viruses [Category] Coronavirus, [키워드] addition Alpha applied approaches bat bats beta coronavirus bioinformatics biological sample collected coronavirus database develop estimates genomes genomic heterogeneity High-throughput sequencing identify impede in viral indicate Insects less loci NGS nucleotide organism plants proportion Protozoa reservoir Sample sequencing data shown utility variety viral pathogen viral pathogens virus viruses [DOI] 10.3390/v13102006 PMC 바로가기 [Article Type] Article
Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNAArticle Published on 2021-10-012022-10-29 Journal: Scientific Reports [Category] COVID-19, [키워드] added biological sample Biological techniques Biomarkers COVID-19 pandemic cultures diagnostic Diseases exacerbate Genome Infectious Bronchitis Virus Influenza virus lab Microbiology Nanoscience and technology nasal Optics and photonics Patient protocol purification risk SARS-CoV-2 throat swab viral outbreak Viral RNA virus virus detection virus particle [DOI] 10.1038/s41598-021-98972-z PMC 바로가기 [Article Type] Article
Developing methods to detect and diagnose chronic traumatic encephalopathy during life: rationale, design, and methodology for the DIAGNOSE CTE Research Project생애 동안 만성 외상성 뇌병증을 탐지하고 진단하는 방법 개발 : 진단 CTE 연구 프로젝트를위한 이론적 근거, 설계 및 방법론Multicenter Study Published on 2021-08-122022-09-10 Journal: Alzheimer's Research & Therapy [Category] MERS, SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] accelerate age anonymized data baseline biological sample biological samples Biomarker Biomarkers Blood blood draw Brain can not chronic Chronic traumatic encephalopathy Clinical research cognitive Cognitive function College football Community Concussion Consensus Contact COVID-19 pandemic detect develop Developing diagnose diagnosed Diagnosis diagnostic Diagnostic Criteria disease Donor Encephalopathy Enrollment Epidemiology evaluated examined facilitate finding Follow-up Football Genetic Head trauma imaging impacts in vivo life Lumbar puncture magnetic resonance imaging male participants Measures mechanisms methodology MRI MRS multicenter National Football League network neurodegenerative Neurodegenerative disease Neuroimaging neurologic neurological Neurological disorders Neuropsychiatric objective organized participant Participation positron emission Positron emission tomography reliability Remote assessment Repetitive head impacts Research Research diagnostic criteria resource Result risk Risk factors Saliva Sample size self-report measure Spectroscopy Subconcussion supplementary material syndrome Tau TES trauma Traumatic brain injury Traumatic encephalopathy syndrome Treatment Validity [DOI] 10.1186/s13195-021-00872-x PMC 바로가기 [Article Type] Multicenter Study
SARS Cov2 outbreak management on a landing helicopter dock: An observational retrospective studyObservational Study Published on 2021-08-012022-10-04 Journal: Infectious diseases now [Category] 임상, [키워드] Analysis Asymptomatic Austere environment biological sample Course COVID-19 demographic data described diagnosed Diagnosis disease French healthy infected patient information landing Landing helicopter dock male management measure median age outbreak Patient performed polymerase chain presymptomatic RANK remained retrospective Retrospective study role SARS CoV2 symptomatic [DOI] 10.1016/j.idnow.2021.06.303 PMC 바로가기 [Article Type] Observational Study
PD-L1 Dysregulation in COVID-19 PatientsImmunology Published on 2021-06-072022-11-01 Journal: Frontiers in Immunology [Category] COVID-19, MERS, SARS, [키워드] Adaptive immune response ARDS biological sample blood sample caused CD4+ T cells clinical study consequence COVID-19 COVID-19 pandemic COVID-19 pathogenesis COVID-19 patient cultures dysregulated dysregulated immune response dysregulation effective epithelial cell Evidence healthy donors healthy individuals hospitalized COVID-19 patient Host immune response identify Immune cell immune checkpoint molecules in vitro Infection inhibitors innate immune response Ligand manifestation Manifestations model system modulation Monocytes Neutrophils Patient PD-L1 Prognosis prognostic prognostic biomarker reached RNA expression SARS-CoV-2 Serum level subset T cell therapeutic approaches tissue tissue biopsy upregulation variety [DOI] 10.3389/fimmu.2021.695242 PMC 바로가기 [Article Type] Immunology
Leveraging natural history biorepositories as a global, decentralized, pathogen surveillance networkOpinion Published on 2021-06-032022-10-29 Journal: PLoS Pathogens [Category] COVID-19, Fulltext, MERS, SARS, 조류인플루엔자, [키워드] America Americas approach association backbone biological sample biorepositories Biorepository Community coronavirus 2 ecological effective growth highlight human infection increasingly Infectious disease International lack natural history pandemic pathogen Predictive Prevent public health replicated Research researchers respiratory reveal risk SARS-CoV-2 specimen Surveillance surveillance network utility vertebrates zoonotic zoonotic spillover [DOI] 10.1371/journal.ppat.1009583 PMC 바로가기 [Article Type] Opinion
Multiplex and on-site PCR detection of swine diseases based on the microfluidic chip systemMethodology Article Published on 2021-03-122022-10-28 Journal: BMC veterinary research [Category] Coronavirus, [키워드] Accuracy amplified biological sample clearance clinical sample Concentration decrease Degradation detect detection system develop disease disease detection Efficiency eight experiment experiments Infection lab Laboratory lowest Microfluidic chips Multiplex and on-site detection nucleic acid pathogenic microorganism PCR performed positive quantitative PCR quarantine raised reduce Result resulting risk specificity Swine disease virus [DOI] 10.1186/s12917-021-02825-w PMC 바로가기 [Article Type] Methodology Article
Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols towards Downstream Isolation and AnalysisCOVID-19 시대의 세포외 소포 분석: 다운스트림 분리 및 분석을 위한 혈청 비활성화 프로토콜에 대한 통찰력Technical Note Published on 2021-03-042022-09-11 Journal: Cells [Category] SARS, 진단, 치료기술, 치료제, [키워드] accompanied adopted Analysis Assessing best biochemical biological sample biological samples biosafety Blotting categories Community complementary COVID-19 COVID-19 crisis crisis Depletion downstream EVs Extracellular vesicles force handling heat inactivation human serum inactivation increase in insight introduced Isolation Laboratory Microarrays miRNA nanoparticle outbreak PCR pre-analytical protocols protocol purity reported SARS-CoV-2 SARS-CoV-2-positive individual serum serum specimen Treatment ultracentrifugation Variability virus inactivation western blotting while [DOI] 10.3390/cells10030544 PMC 바로가기 [Article Type] Technical Note
Inactivation and Elimination of SARS-CoV-2 in Biosamples Using Simple Fixatives and UltrafiltrationProtocol Published on 2021-02-272022-10-30 Journal: Methods and Protocols [Category] COVID-19, [키워드] accelerated affected Analysis biocontainment biological sample BSL-3 BSL2 cause Cell Cell culture Complete conducted COVID-19 cut-off downstream analysis effective Effectiveness eliminating Elimination Ethanol Ex vivo fixative formalin handling Health host cell in vitro in vivo model inactivated inactivation individual Infection infection risk kDa Laboratory limit lung tissue lysates media membrane membrane filter molecular weight neutral buffered formalin pandemic paraformaldehyde pathogenesis of SARS-CoV-2 pathogenicity pathological analysis plaque forming units reduce Research respiratory Safe SARS-CoV-2 SARS-CoV-2 infected cell significantly simple standard procedure subsequent tissue Treatment Ultrafiltration undetectable viral inoculum virus [DOI] 10.3390/mps4010018 PMC 바로가기 [Article Type] Protocol
Impact of intravenous lidocaine on clinical outcomes of patients with ARDS during COVID-19 pandemia (LidoCovid): A structured summary of a study protocol for a randomised controlled trialCOVID-19 대유행 중 ARDS 환자의 임상 결과에 대한 정맥내 리도카인의 영향(LidoCovid): 무작위 대조 시험을 위한 연구 프로토콜의 구조화된 요약Letter Published on 2021-02-112022-09-10 Journal: Trials [Category] MERS, 임상, [키워드] 1:1 24 hour 24 hours acute renal failure acute respiratory distress acute respiratory distress syndrome Admission adverse event adverse events age albumin All participant All participants Anti-inflammatory effects approved aPTT ARDS Atrial fibrillation Bacterial Berlin Beta Bicarbonate biological sample biological samples blinded Blinding blockers blood count bolus bronchospasm cardiac rhythm Cardiogenic shock Care care providers Child bearing clinical Clinical data Clinical outcome clinical study collected Commission comparator complete blood count Complication computer-generated Concentration conducted Consumption control group cough COVID-19 COVID-19 pneumonia Covid-19 subgroup CRP D-dimers delirium diagnostic Disease progression disorders dissemination distribution Distribution ratio dosage Effect element ethics committee evaluate the effect evaluated Evolution experimental group extubation ferritin fibrinogen FiO2 followed by following form France French Gas exchange HCG Hospitalized hypnotics ICU IL-6 Ileus Impact incidence include inclusion criteria Inflammation Inflammatory marker inflammatory markers intensive care intensive care unit interval intervention group intravenous Intubated investigator LDH Length lidocaine Local local anaesthetics Local anesthetics Lymphocytes moderate moderate to severe Modification molecular mechanisms of pharmacological action Mortality NaCl number objective Older opioids outcome Pandemia PaO2 PaO2/FiO2 ratio parallel group participant Patient patients with ARDS PCT Phase 3 Placebo placebo-controlled Platelets Pneumonia pneumopathy Pneumothorax Prevent Primary outcome protection Protective protocol QTc random randomisation randomised randomised controlled trial randomize Randomized Randomly receive Recovery time recruitment Registered Renal Dialysis renal failure report respiratory distress RT-PCR Sample size SARS-CoV2 infection secondary endpoint Secondary endpoints sedated Severe acute respiratory syndrome severe ARDS Shock Social Security Social security system sore throat status Stratification stroke Study protocol supplementary material surgical syndrome tetraplegia thromboembolic Thromboembolic event timepoint Toxicity Treatment Trial Trial registration triglycerides troponin two group two groups university vasopressors Ventilation ventilator ventilator-free day Ventilator-free days website women written Informed Consent [DOI] 10.1186/s13063-021-05095-x PMC 바로가기 [Article Type] Letter