Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnosticsArticle Published on 2022-06-282023-07-09 Journal: Proceedings of the National Academy of Sciences of [Category] COVID19(2023년), [키워드] CRISPR-Cas12a Nucleic acid detection one-pot assay. PC linker RPA [DOI] 10.1073/pnas.2202034119 PMC 바로가기
CLIPON: A CRISPR-Enabled Strategy that Turns Commercial Pregnancy Test Strips into General Point-of-Need Test DevicesArticle Published on 2022-03-142023-07-07 Journal: Angewandte Chemie (International ed. in English) [Category] COVID19(2023년), [키워드] CRISPR-Cas12a Nucleic acid detection Point-of-Care Diagnostic Pregnancy Strips SARS-CoV-2 [DOI] 10.1002/anie.202115907
Instrument-free, CRISPR-based diagnostics of SARS-CoV-2 using self-contained microfluidic system자급식 미세유체 시스템을 사용하여 기기가 필요 없는 CRISPR 기반 SARS-CoV-2 진단Article Published on 2022-03-012022-09-11 Journal: Biosensors & bioelectronics [Category] MERS, SARS, 진단, 치료기술, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 cleavage clinically coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CRISPR CRISPR-Cas12a Critical detect Device diagnostics Diseases electricity hand Hand warmer Infectious diseases instrument Instrument-free molecular diagnostics isothermal amplification lateral flow molecular Nasopharyngeal swab operation other infectious diseases platform point-of-care Rapid reagent respiratory SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA Self-contained microfluidic chip. sensitivity severe acute respiratory syndrome Coronavirus specificity Treatment [DOI] 10.1016/j.bios.2021.113865 PMC 바로가기 [Article Type] Article
A smartphone-based visual biosensor for CRISPR-Cas powered SARS-CoV-2 diagnosticsCRISPR-Cas 기반 SARS-CoV-2 진단을 위한 스마트폰 기반 시각 바이오센서Article Published on 2022-01-012022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 aggregation applied changes clinical clinical samples clinically color coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CRISPR CRISPR-Ca CRISPR-Cas12a cross-reactivity Degradation detect develop diagnostics evidenced eye gold Gold nanoparticle Gold nanoparticles imperative limit of detection nucleic acid nucleic acids pandemic positive provide provided pseudovirus pseudoviruses qPCR RNA robust SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 pseudoviruses selective single-stranded DNA Smartphone Smartphone-based diagnostics the SARS-CoV-2 transcribed triggered vectors Visual Visual biosensor. [DOI] 10.1016/j.bios.2021.113646 PMC 바로가기 [Article Type] Article
Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay디지털 웜 스타트 CRISPR 분석을 사용하여 임상 샘플에서 SARS-CoV-2의 민감한 정량적 검출Article Published on 2021-07-152022-09-11 Journal: Biosensors & bioelectronics [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification ChIP clinical sample clinical samples clinically coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CRISPR CRISPR-Cas12a demonstrated detect Digital Digital warm-start CRISPR assay early diagnosis facilitate initiated Nucleic acid quantification overcome Premature quantification Quantitative RNA extraction room temperature Saliva SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA sensitive severe acute respiratory syndrome Coronavirus swab sample temperature Treatment [DOI] 10.1016/j.bios.2021.113218 PMC 바로가기 [Article Type] Article
Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimensComparative Study Published on 2021-04-012023-06-19 Journal: Journal of virological methods [Category] MERS, SARS, [키워드] clinical specimen CRISPR-Cas12a performance SARS-CoV-2 [DOI] 10.1016/j.jviromet.2021.114092 PMC 바로가기 [Article Type] Comparative Study
SARS-CoV-2 Direct Detection Without RNA Isolation With Loop-Mediated Isothermal Amplification (LAMP) and CRISPR-Cas12Medicine Published on 2021-02-172022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] clinical sample confirmed case COVID-19 COVID-19 outbreak CRISPR-Cas12a current detection Diagnosis diagnostic diagnostic protocol Direct epidemiological equipment evaluated faster LAMP limit of detection Loop-mediated isothermal amplification management Palindromic Patient Protein qRT-PCR Real-time PCR reported RNA RNA extraction SARS-CoV-2 Sensitivity and specificity specificity thermocycler Viral RNA [DOI] 10.3389/fmed.2021.627679 PMC 바로가기 [Article Type] Medicine
An Update on Molecular Diagnostics for COVID-19COVID-19에 대한 분자 진단에 대한 업데이트Review Published on 2020-11-102022-08-31 Journal: Frontiers in Cellular and Infection Microbiology [Category] MERS, SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] Accuracy acute respiratory syndrome coronavirus affected affecting anatomical approach approaches arising assistance Asymptomatic carrier Asymptomatic carriers Care caused changed China Chinese patient Chinese patients Collecting Complete contagious contagious disease coronavirus coronavirus 2 coughing COVID-19 crisis CRISPR CRISPR-Cas12a Critical detect Diagnosis diagnostic diagnostic procedure diagnostic procedures diagnostics disease effective flu-like symptoms genome sequence house humans IgA IgG IgM imperative Infection Infectious disease lateral flow majority market Molecular diagnosis Other outbreak pandemic Particle pathogen pathogen detection Pathogens Patient POC Point positive respiratory Respiratory system reverse transcription-PCR RT-PCR SARS-CoV-2 scenario serum antibodies serum antibody severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 severe pneumonia SHERLOCK shown sneezing Spread Symptom Transmission virus widespread [DOI] 10.3389/fcimb.2020.560616 PMC 바로가기 [Article Type] Review