One-step colorimetric isothermal detection of COVID-19 with AI-assisted automated result analysis: A platform model for future emerging point-of-care RNA/DNA disease diagnosisResearch article Published on 2022-11-012022-10-05 Journal: Talanta [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] Accuracy Analysis analyzed approach automated Colorimetric RT-LAMP Complete Concordance COVID-19 COVID-19 testing cycle threshold Detection limit diagnosis of COVID-19 Disease diagnosis DNA downstream ENhance exhibited faster feasibility Interpretation isothermal LAMP-DETR limit machine learning majority observation offer pandemic pathogen performed Perspective platform point-of-care RT-LAMP RT-qPCR SARS-CoV-2 sensitivity specificity [DOI] 10.1016/j.talanta.2022.123375 [Article Type] Research article
Temporal Dynamics of Nasopharyngeal and Tracheal Severe Acute Respiratory Syndrome Coronavirus 2 Cycle Thresholds in Coronavirus Disease 2019 Patients With TracheostomyArticle Published on 2022-10-292022-11-15 Journal: Clinical Infectious Diseases: An Official Publicat [Category] COVID19(2023년), SARS, 진단, [키워드] analyzed coronavirus 2 COVID cycle cycle threshold cycle thresholds Dynamics nasopharyngeal patients with COVID-19 PCR remained respiratory SARS-CoV-2 Temporal tracheal tracheostomy virus [DOI] 10.1093/cid/ciac316 PMC 바로가기
Comparison of the SARS-CoV-2 BD Veritor Nasal Antigen Test with Nasopharyngeal Reverse Transcription-PCR in Symptomatic PatientsArticle Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, [키워드] Accuracy addition analyzed Antigen asymptomatic patient coronavirus COVID-19 cycle threshold Diagnosis diagnostic evaluated genes encoding immunochromatographic Infection information Interpretation methodology nasopharyngeal oropharyngeal swab Patient performed POC POC test point-of-care point-of-care test point-of-care testing provide rapid antigen test Rapid test Rapid tests reduce reproducibility reverse transcription-PCR RT-PCR Safe saline solution Sample SARS-CoV-2 sensitivity symptom onset symptomatic Symptomatic patients temperature Test tested the SARS-CoV-2 Variation Veritor test [DOI] 10.1128/spectrum.00190-22 PMC 바로가기
[Potential usefulness of CT as a marker of persistence of positive PCR in patients with COVID-19]Article Published on 2022-10-262023-07-10 Journal: Revista Latino-Americana de Enfermagem [Category] COVID19(2023년), [키워드] cycle threshold Persistence in nasopharyngeal secretions SARS-CoV-2 Spain
Reverse Transcription-Loop-Mediated Isothermal Amplification-CRISPR-Cas13a Technology as a Promising Diagnostic Tool for SARS-CoV-2Article Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome caused change clinical samples coronavirus coronavirus disease COVID-19 CRISPR-Ca CRISPR-Cas CRISPR-Cas13 cycle threshold deaths develop Diagnosis diagnostic Diagnostic method early diagnosis effort exhibit highlighting isothermal isothermal amplification maintain Molecular diagnosis nasopharyngeal sample Negative predictive value New coronavirus NPV pandemic performed persist Positive predictive value PPV Prevalence protocol reaction required researcher RNA extraction RNA molecules RT-LAMP SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 virus sensitivity Sensitivity and specificity single nucleotide single-stranded specificity technology viral loads [DOI] 10.1128/spectrum.02398-22 PMC 바로가기
A Multicenter Clinical Diagnostic Accuracy Study of SureStatus, an Affordable, WHO Emergency Use-Listed, Rapid, Point-Of-Care Antigen-Detecting Diagnostic Test for SARS-CoV-2Article Published on 2022-10-262022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] 95% confidence interval access Accuracy acute respiratory syndrome Ag-RDT Ag-RDTs Analysis Antigen-detecting rapid diagnostic tests B.1.1.7 characterized circulating clinical Combination complement coronavirus COVID-19 Critical cycle threshold demonstrated diagnostic Emergency Emergency use evaluated facilitate Factor Germany gold standard highlight independent India multicenter nasopharyngeal Oropharyngeal overcome participant Participants performance performed point-of-care positive post-symptom onset questionnaire raised Rapid Rapid diagnostic test recruited resources reverse transcription-PCR RT-PCR RT-PCR testing SARS-CoV-2 SARS-COV-2 infection sensitivity specificity subgroup subgroups Support SUS Swab symptom duration symptom onset Symptoms Test Transmission variant Viral load was used WHO widespread World Health Organization [DOI] 10.1128/spectrum.01229-22 PMC 바로가기
Differential Infectivity of Original and Delta Variants of SARS-CoV-2 in Children Compared to AdultsArticle Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome Adults age aliquot B.1.617.2 Canada characteristic children circulating circulating SARS-CoV-2 variants coronavirus COVID-19 Culture cycle threshold Delta delta variant demographics determine dose driver implicated in viral Infection infectious titers infective Infectivity mechanism Mutation Nasopharyngeal swab nasopharyngeal swab sample original pediatric positive predominant provided RT-PCR SARS-CoV-2 SARS-CoV-2 RT-PCR screened statistical analysis susceptible TCID TCID50 TCID50. tissue Transmission variant variants of concern variety Viral RNA VoC [DOI] 10.1128/spectrum.00395-22 PMC 바로가기
Clinical predictors and RT-PCR profile of prolonged viral shedding in patients with SARS-CoV-2 Omicron variant in Shanghai: A retrospective observational studyArticle Published on 2022-10-242022-11-16 Journal: Frontiers in Public Health [Category] COVID19(2023년), SARS, 변종, [키워드] acute respiratory syndrome Admission Analysis clearance clinical Comorbidity Coronary heart disease coronavirus Ct values cycle threshold determinant diabetes equation evaluate Frame Generalized group hospitalized patient hypertension Infection male material median age multivariable analysis N gene nasopharyngeal nucleocapsid protein omicron ORF ORF 1ab gene Patient patients with SARS-CoV-2 positive predictor predictors prolonged SARS-CoV-2 RNA shedding. prolonged viral shedding proportion receiving remained retrospective Retrospective study RT-PCR SARS-CoV-2 SARS-CoV-2 Omicron variant SARS-CoV-2 RNA significantly single-center tested the timing vaccination Vaccinations Vaccine variant viral clearance viral RNA clearance Viral RNA shedding [DOI] 10.3389/fpubh.2022.1015811 PMC 바로가기
Measurement of Severe Acute Respiratory Syndrome Coronavirus 2 Antigens in Plasma of Pediatric Patients With Acute Coronavirus Disease 2019 or Multisystem Inflammatory Syndrome in Children Using an Ultrasensitive and Quantitative ImmunoassayArticle Published on 2022-10-122022-11-15 Journal: Clinical Infectious Diseases: An Official Publicat [Category] COVID19(2023년), SARS, 치료기술, [키워드] 24 hour acute COVID-19 acute respiratory syndrome Antigen antigenemia Blood blood collection blood draw CDC criteria children Concentration contribute Control coronavirus coronavirus 2 coronavirus disease correlated COVID-19 COVID-19 patient cycle threshold detection diagnostic disease severity enrolled highest hospital immunoassay Inflammatory Inpatient measurement Meso Scale Discovery MIS-C nasopharyngeal Nasopharyngeal swab negative RT-PCR Pathogenesis Patient patients Pediatric patient plasma positive quantified ranged recent respiratory RT-PCR SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 nucleocapsid SARS-CoV-2 RT-PCR SARS-CoV-2 spike sensitivity serology syndrome tested the patient ultrasensitive immunoassay. undetectable was collected [DOI] 10.1093/cid/ciac160 PMC 바로가기
Comparison and Harmonization of Different Semi-Automated and Automated qRT-PCR Assays in the Assessment of SARS-CoV-2Article Published on 2022-10-122022-11-15 Journal: Viruses [Category] COVID19(2023년), SARS, 치료기술, [키워드] Analysis Applying assays assessment automated carried comparability Concentration COVID-19 cycle threshold Cycle threshold value detectable diagnostics FIVE harmonization highlight identify infected individuals Isolation Laboratory lowest mandatory oropharyngeal swab Patient qRT-PCR qRT-PCR assay Qualitative Quantitative quantitative reverse transcriptase-polymerase chain reaction RIDA SARS-CoV-2 SARS-CoV-2 qRT-PCR sensitivity significant difference significantly the median Viral load [DOI] 10.3390/v14102239 PMC 바로가기