Effect of selected wastewater characteristics on estimation of SARS-CoV-2 viral load in wastewater폐수 내 SARS-CoV-2 바이러스 부하 추정에 대한 선택된 폐수 특성의 영향Article Published on 2022-01-012022-09-11 Journal: Environmental research [Category] SARS, 치료기술, [키워드] Adaptive neuro-fuzzy inference system model ammonia amplification approach association Chain Reaction characteristic Characteristics chemical oxygen demand Concentration COVID-19 COVID-19 infections dependent on determine droplet droplet digital PCR Effect Epidemiology flow rate highest Increasing Inference investigated observation Other oxygen oxygen demand parameter parameters permanganate physico-chemical properties physico-chemical property plant plants polymerase chain polymerase chain reaction quantification ranged RNA RNA concentrations robust SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 viral load selected Surveillance System the SARS-CoV-2 total solids Treatment Viral RNA was recorded wastewater Wastewater-based epidemiology [DOI] 10.1016/j.envres.2021.111877 PMC 바로가기 [Article Type] Article
Impact of Full Vaccination with mRNA BNT162b2 on SARS-CoV-2 Infection: Genomic and Subgenomic Viral RNAs Detection in Nasopharyngeal Swab and Saliva of Health Care WorkersArticle Published on 2021-08-142022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] analysed Analysis Asymptomatic B.1.1.7 variant baseline BNT162b2 Concordance coronavirus Course cumulative detection droplet digital PCR eight expression Frequency HCW HCWs Health care worker hospitals identify Impact incidence individual individuals infected individuals Infection investigated majority MONITOR mRNA BNT162b2 Mutation N501Y NPs performed positive provided real time real time RT-PCR real-time RT-PCR receiving resulting RNA RT-PCR Saliva saliva specimen SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 surveillance specimens subgenomic subjects symptomatic symptomatic cases tested Transmission vaccination Vaccine vaccinee variants Viral load Viral mutations Viral RNA was measured worker [DOI] 10.3390/microorganisms9081738 PMC 바로가기 [Article Type] Article
Application of One-Step Reverse Transcription Droplet Digital PCR for Dengue Virus Detection and Quantification in Clinical SpecimensArticle Published on 2021-04-012023-08-30 Journal: Diagnostics [Category] E형 간염, [키워드] dengue virus droplet digital PCR virus detection Virus quantification [DOI] 10.3390/diagnostics11040639 PMC 바로가기 [Article Type] Article
Droplet digital PCR of viral DNA/RNA, current progress, challenges, and future perspectivesReview Published on 2021-03-112022-11-01 Journal: Journal of medical virology [Category] COVID-19, [키워드] addition Concentration digital PCR DNA droplet droplet digital PCR Efficacy feature in viral include inhibitor microfluidic molecular PCR PCR amplification Perspective Poisson distribution polymerase chain proportion quantification reproducibility RNA Sensitivity and specificity standard curve Tolerance virus [DOI] 10.1002/jmv.26846 PMC 바로가기 [Article Type] Review
Analysis of viral load in different specimen types and serum antibody levels of COVID-19 patientsCOVID-19 환자의 다양한 검체 유형 및 혈청 항체 수준의 바이러스 부하 분석Research Published on 2021-01-072022-09-10 Journal: Journal of Translational Medicine [Category] 진단, 치료기술, 치료법, [키워드] affected anal swab anal swabs anti-S Anti-spike antibody antibody levels be positive best binding domain Blood caused collected correlation Course COVID-19 COVID-19 patient death detection rate digital PCR disease domain droplet digital PCR ELISA Factors False negative false negative results followed by global pandemic had no highest IgG antibodies IgG antibody IgM and IgG information Nasopharyngeal swab nasopharyngeal swabs no correlation Other Patient positive Protein Result Saliva SARS-CoV-2 detection serum antibody serum sample specimen swabs symptoms onset tested Urine urine specimens Viral Viral load was measured [DOI] 10.1186/s12967-020-02693-2 PMC 바로가기 [Article Type] Research
Developing multiplex ddPCR assays for SARS-CoV-2 detection based on probe mix and amplitude based multiplexingArticle Published on 2021-01-012023-06-19 Journal: Expert review of molecular diagnostics [Category] MERS, SARS, [키워드] COVID-19 Diagnosis droplet digital PCR multiplex assays RT-PCR SARS-CoV-2 [DOI] 10.1080/14737159.2021.1865807 PMC 바로가기
Long-Term SARS-CoV-2 Infection Associated with Viral Dissemination in Different Body Fluids Including Bile in Two Patients with Acute CholecystitisCase Report Published on 2020-11-232022-10-29 Journal: International Review of Cell and Molecular Biology [Category] COVID-19, [키워드] assays bile biological sample Body body compartment body fluid Cholecystitis clinical manifestations collected contribute declined different time point digital PCR droplet digital PCR dropped highlight Including Infection nasopharyngeal Nasopharyngeal swab negativity onset of symptoms Patient patients plasma plasma sample provoke qRT-PCR quantification Rectal swabs respiratory sample RNA quantification RT-PCR SARS-CoV-2 SARS-CoV-2 positive patient SARS-CoV-2 RNA Symptom undetectable Urine Viral RNA [DOI] 10.3390/life10110302 PMC 바로가기 [Article Type] Case Report
Evaluation of droplet digital PCR for quantification of SARS-CoV-2 Virus in discharged COVID-19 patients퇴원한 코로나19 환자에서 SARS-CoV-2 바이러스 정량을 위한 비말 디지털 PCR 평가Research Paper Published on 2020-11-152022-09-01 Journal: Aging (Albany NY) [Category] SARS, 진단, 치료기술, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition analyzed be positive blind clinical sample clinical samples coronavirus coronavirus disease COVID-19 COVID-19 diagnosis COVID-19 patient cutoff value detect diagnosis of COVID-19 Digital digital PCR discharge discharged discharged criteria discharged patient discharged patients droplet droplet digital PCR eight gold gold standard help IMPROVE in some infected patient Inpatient Misdiagnosis outbreak Patient PCR positive positive sample qPCR quantification Quantitative quantitative real time PCR real time real time PCR reduce relapsing respiratory retained SARS-CoV-2 selected Sensitivity and specificity significantly Spread synchronous Viral Viral load virus [DOI] 10.18632/aging.104020 PMC 바로가기 [Article Type] Research Paper
Comparing the performance of conventional PCR, RTQ-PCR, and droplet digital PCR assays in detection of Shigellaarticle Published on 2020-06-302024-09-01 Journal: Molecular and Cellular Probes [Category] 세균성이질, [키워드] conventional PCR droplet digital PCR foodborne infections quantitative real-time PCR Shigella spp. detection [Article Type] article
ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimensResearch Article Published on 2020-06-072022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, MERS, SARS, [키워드] Accuracy clinical detection Clinical diagnosis complement convalescent criteria cut off detect diagnose diagnostic digital PCR discharge droplet digital PCR False negative feasibility Follow-up gold standard IMPROVE likelihood ratio limit of detection limitation negative RT-PCR NLR NPV patients patients of COVID-19 PCR positive PPV real time reduce reported RT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA sensitivity significantly lower specificity specimen Transmission Viral load was used [DOI] 10.1080/22221751.2020.1772678 PMC 바로가기 [Article Type] Research Article