Rapid adaptation and continuous performance evaluation of SARS-CoV-2 envelope gene (E-gene) real-time RT-PCR assays to support the hospital surge in test demandArticle Published on 2021-03-012023-07-12 Journal: Journal of medical virology [Category] SARS, [키워드] E-gene real-time RT-PCR SARS-CoV-2 [DOI] 10.1002/jmv.26660
Comparative performance of SARS-CoV-2 real-time PCR diagnostic assays on samples from Lagos, Nigeria나이지리아 라고스의 샘플에 대한 SARS-CoV-2 실시간 PCR 진단 분석의 비교 성능Comparative Study Published on 2021-02-042022-09-10 Journal: PLoS ONE [Category] MERS, 진단, 치료기술, [키워드] affected approvals assays Comparative Ct value cut-off value determine Diagnosis diagnostic diagnostics E-gene Emergency use expected Factors FIVE house identify indication Infection Laboratory Lagos Nasopharyngeal swab nasopharyngeal swab sample Nasopharyngeal swab samples negative sample Nigeria PCR assays positive sample Qiagen qPCR assays Real-time PCR reported reverse transcriptase RNA SARS-CoV-2 SARS-COV-2 infection seeking Spread tested Testing the SARS-CoV-2 transcriptase Viral Viral RNA viral RNA extracted virus was done was performed [DOI] 10.1371/journal.pone.0246637 PMC 바로가기 [Article Type] Comparative Study
Analysis of COVID-19-Related RT-qPCR Test Results in Hungary: Epidemiology, Diagnostics, and Clinical OutcomeMedicine Published on 2021-01-262022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] age and sex clinical conducted Coronavirus infection Coronavirus infections COVID-19 COVID-19 case diagnose disease E-gene epidemiological Epidemiology first test first-wave Hungary ICU admission immunologically IMPROVE in-hospital mortality Infection initially asymptomatic patient intensive care Mortality naïve no difference outcome pandemic patients PCR diagnostics PCR positivity PCR test performed positive required Result Retrospective analysis RT-qPCR RT-qPCR test SARS-CoV-2 statistical analysis Surveillance survived Test tested Testing [DOI] 10.3389/fmed.2020.625673 PMC 바로가기 [Article Type] Medicine
Detection of severe acute respiratory coronavirus virus 2 (SARS-CoV-2) in outpatients: A multicenter comparison of self-collected saline gargle, oral swab, and combined oral–anterior nasal swab to a provider collected nasopharyngeal swab외래 환자에서 중증 급성 호흡기 코로나바이러스 바이러스 2(SARS-CoV-2) 검출: 자가 채취 식염수 가글, 구강 면봉 및 구강-전 비강 면봉을 제공자에서 수집한 비인두 면봉과 다기관 비교Multicenter Study Published on 2021-01-132022-09-10 Journal: Infection Control and Hospital Epidemiology [Category] MERS, SARS, 진단, 치료기술, [키워드] 95% CI adjusted Canada Characteristics collected coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 Cycle threshold value demonstrated detection disadvantage Disadvantages E-gene median multicenter nasal swab Nasopharyngeal swab oral swab oral swabs overcome populations Positive test reference standard respiratory Respiratory Coronavirus SARS-CoV-2 SARS-CoV-2 detection sensitivity specimen Spread Swab the SARS-CoV-2 Toronto virus were assessed [DOI] 10.1017/ice.2021.2 PMC 바로가기 [Article Type] Multicenter Study
Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNAEditor's Choice Published on 2020-09-052022-10-29 Journal: Journal of Medical Microbiology [Category] COVID-19, SARS, [키워드] alleviate average bypassing CDC clinical sample Combination conducted COVID-19 detect diagnostic diagnostics Dilution E-gene enzyme evaluated FAM FDA implementation intervals isothermal amplification Laboratory medium Nasopharyngeal swab nasopharyngeal swabs nucleocapsid primer required resource Result reverse transcription RNA extraction kit RNA purification RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 RNA SARS-CoV-2 virus screened Sensitivity and specificity specimen TCID the SARS-CoV-2 thermocycler thermocyclers Transport tube universal transport media UTM validation virus detection [DOI] 10.1099/jmm.0.001238 PMC 바로가기 [Article Type] Editor's Choice
Post-mortem viral dynamics and tropism in COVID-19 patients in correlation with organ damage장기 손상과 상관관계가 있는 COVID-19 환자의 사후 바이러스 역학 및 친화성Original Article Published on 2020-08-202022-09-10 Journal: Virchows Archiv [Category] MERS, 진단, 치료기술, 치료법, 치료제, [키워드] age alveolar damage amplification Autopsy bile both lungs Brain bronchial glands bronchopneumonia can not changes correlation COVID-19 COVID-19 patient Ct values cycle threshold death Deceased diagnosed with COVID-19 Diffuse alveolar damage E-gene Gallbladder immunohistochemistry Immunohistochemistry bowel Infarction infected individual infected individuals intestinal epithelium intestine less lung Lungs males median moderate nucleic acid nucleocapsid protein ORF1a/b organ organ damage Patient PCR persistence Pneumocytes positive Post-mortem Potential primer Primers Protein respiratory respiratory tract RNA SARS-CoV-2 SARS-CoV-2 RNA significant increase structural protein supplementary material Swab swabs throat swab thrombosis tissue damage Viral viral dynamics Viral RNA virus [DOI] 10.1007/s00428-020-02903-8 PMC 바로가기 [Article Type] Original Article
Genetic Diversity Among SARS-CoV2 Strains in South America may Impact Performance of Molecular DetectionArticle Published on 2020-07-172022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] Affect annealing binding binding site Brazil China conserved coronavirus 2 database death detection Diversity E-gene Ecuador gene E gene N gene RdRp Impact in silico analysis individual infecting Molecular diagnosis nucleotide PCR performance primer-probe set RdRp gene reported respiratory Result SARS-CoV-2 SARS-CoV-2 genome SARS-CoV2 sensitivity South South America Spread strain Variation virus whole genome sequence Wuhan [DOI] 10.3390/pathogens9070580 PMC 바로가기 [Article Type] Article
Evaluation of the commercially available LightMix® Modular E-gene kit using clinical and proficiency testing specimens for SARS-CoV-2 detectionArticle Published on 2020-05-272022-11-01 Journal: Journal of Clinical Virology [Category] COVID-19, MERS, SARS, [키워드] Algorithm analytical sensitivity assays Characteristics correlation CoV COVID-19 Culture cycle diagnostic Dilution E-gene evaluate evaluated Evaluation infection control LightMix E-gene limit of detection log management objective Patient PCR performance performed Rapid required Result RT-PCR assays SARS-CoV-2 SARS-CoV-2 detection sensitivity specimen TCID threshold threshold value Variation were used Wuhan [DOI] 10.1016/j.jcv.2020.104476 PMC 바로가기 [Article Type] Article
Comparison of seven commercial RT-PCR diagnostic kits for COVID-19Article Published on 2020-05-082022-10-04 Journal: Journal of Clinical Virology [Category] MERS, SARS, 진단, [키워드] acute respiratory syndrome Altona Diagnostics BGI clinical sample clinical samples clinical sensitivity coronavirus COVID-19 COVID-19 in patient cross-reactivity detection rate diagnostic diagnostics E-gene evaluate expected Final globe human coronavirus in vitro diagnostics individual limit of detection measure molecular nCOV-2019 Novel coronavirus PCR PCR efficiency positive PrimerDesign R-Biopharm real-time reverse transcriptase reduce respiratory viral infection RNA RT-PCR RT-PCR kit SARS-CoV-1 SARS-CoV-2 selected serial dilution Seven specificity Spread Variation Viral RNA [DOI] 10.1016/j.jcv.2020.104412 PMC 바로가기 [Article Type] Article
Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay KitVirology Published on 2020-04-082022-10-30 Journal: Journal of Clinical Microbiology [Category] COVID-19, MERS, SARS, [키워드] acute respiratory syndrome Analysis CDC center clinical Cluster Comparative Control coronavirus country Course cross-reactivity described detection division E-gene effort Euro evaluated information Laboratory N2 N2 set other respiratory virus performance performed prevention primer primer-probe set probe protocol public health crisis rapid increase SARS-CoV-2 SARS-CoV-2 testing Seattle sensitive assay Seven spread to tested The United States University of Washington Virology virus Washington [DOI] 10.1128/JCM.00557-20 PMC 바로가기 [Article Type] Virology