Improving RT-LAMP detection of SARS-CoV-2 RNA through primer set selection and combination프라이머 세트 선택 및 조합을 통한 SARS-CoV-2 RNA의 RT-LAMP 검출 개선Article Published on 2022-04-012022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] adoption approach assays breadth Combination conditions Detection sensitivity Diagnostic method Effect evaluate expand identify Improving isothermal amplification knowledge LAMP Loop-mediated isothermal amplification molecular nucleic acid nucleic acid testing observé primer primer set primer sets prominence RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA sensitivity Surveillance targets Transcription utility while [DOI] 10.1371/journal.pone.0254324 PMC 바로가기 [Article Type] Article
Importance of sample input volume for accurate SARS-CoV-2 qPCR testing정확한 SARS-CoV-2 qPCR 검사를 위한 시료 투입량의 중요성Article Published on 2022-03-222022-09-11 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 causative agent Concentration Consistency coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 sample Ct-value false-negative Importance Inconclusive initial input nucleic acid nucleic acid testing Occurrence outcome pandemic Poisson Poisson distribution Probability purified RNA qPCR qPCR assay qPCR test Quantitative random resulting reverse transcription PCR RNA RNA molecule robustness Sample Sample input. SARS-CoV-2 SARS-CoV-2 testing Sensitivity and specificity severe acute respiratory syndrome Coronavirus significantly Viral load Volume was used [DOI] 10.1016/j.aca.2022.339585 PMC 바로가기 [Article Type] Article
Diagnostic techniques for COVID-19: A mini-reviewCOVID-19 진단 기술: 미니 리뷰Review Published on 2022-03-012022-09-11 Journal: Journal of virological methods [Category] SARS, 변종, 진단, 치료기술, 치료제, [키워드] Accuracy antigen detection Asymptomatic carrier Asymptomatic carriers auxiliary Combination Computed tomography country COVID-19 COVID-19 diagnostic assay COVID-19 diagnostic assays COVID-19 patient COVID-19 patients CT imaging Diagnosis diagnosis of COVID-19 diagnostic diagnostics Early detection early diagnosis early stage immunological Immunological methods IMPROVE in some indicate Isolation nucleic acid nucleic acid testing nucleic acid tests pandemic positive result presence of antibody qPCR quantitative PCR reported respiratory infectious disease resulting SARS-CoV-2 SARS-CoV-2 virus Serological testing serum antibodies serum antibody Spread symptomatic patient Symptomatic patients the SARS-CoV-2 virus Viral load virus virus detection virus genome Wuhan Wuhan, China [DOI] 10.1016/j.jviromet.2021.114437 PMC 바로가기 [Article Type] Review
Low rate of RNAemia in blood donations collected during the first wave of COVID-19 in FranceArticle Published on 2022-03-012023-07-07 Journal: Transfusion [Category] COVID19(2023년), [키워드] Blood donors nucleic acid testing plasma RNAemia SARS-CoV-2 [DOI] 10.1111/trf.16812
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots오픈 소스 피펫팅 로봇을 활용한 효율적인 SARS-CoV-2 정량적 역전사효소 PCR 타액 진단 전략Article Published on 2022-02-112022-09-11 Journal: Journal of visualized experiments : JoVE [Category] COVID19(2023년), SARS, 치료기술, [키워드] automated Automation average Chain Reaction Clemson University clinical testing Community comparable complex Concentration COVID-19 pandemic diagnostic diagnostic testing efficient epidemiological handling Health Health crisis introduced invasive less Mortality multiplex RT-qPCR nasopharyngeal swabs nucleic acid nucleic acid testing Participants PCR physically polymerase chain polymerase chain reaction Population population surveillance Quantitative reagent Reporting Research reverse transcriptase robot RT-qPCR Saliva SARS-CoV-2 Sensitivity and specificity shown Strategy Supply chain Surveillance thermocycler thermocyclers transcriptase Transmission Viral RNA [DOI] 10.3791/63395 PMC 바로가기 [Article Type] Article
[Study on Reentry Evaluation Mode for Blood Donors Used to be HBV Reactive in Jiangsu Province]Article Published on 2022-02-012023-06-12 Journal: Zhongguo shi yan xue ye xue za zhi [Category] B형 간염, [키워드] donor reentry electrochemiluminescence immunoassay hepatitis B surface antigen nucleic acid testing Risk assessment [DOI] 10.19746/j.cnki.issn.1009-2137.2022.01.044
Challenges Faced in Large-Scale Nucleic Acid Testing during the Sudden Outbreak of the B.1.617.2 (Delta)B.1.617.2(델타)의 갑작스러운 발병 동안 대규모 핵산 테스트에서 직면한 과제Article Published on 2022-01-292022-09-10 Journal: International Journal of Environmental Research an [Category] 변종, [키워드] acid analyzed approach B.1.617.2 challenge challenges China Community conducted Containment cooperation country COVID-19 Delta delta variant diagnostic testing Efficiency effort eight empirical evidence Evidence Government group Health IMPROVE infection risk involved local government local governments medical resource nucleic acid nucleic acid testing outbreak participant Perspective positive professionals public health Purposive sampling qualitative analysis qualitative research respond selected Seven Spread sudden Support Testing the B.1.617.2 variant virus Volunteer [DOI] 10.3390/ijerph19031573 PMC 바로가기 [Article Type] Article
Monitoring the SARS-CoV-2 pandemic: screening algorithm with single nucleotide polymorphism detection for the rapid identification of established and emerging variantsSARS-CoV-2 대유행 모니터링: 확립된 변이체 및 신규 변이체의 신속한 식별을 위한 단일 염기 다형성 검출을 통한 스크리닝 알고리즘Article Published on 2022-01-012022-09-11 Journal: Clinical microbiology and infection : the official [Category] SARS, 변종, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Algorithm Alpha applied assessment Beta Chain Reaction Concordance coronavirus coronavirus disease Coronavirus disease 2019 Delta determine disease E484K Epsilon Eta evaluate evaluated failure flexible Gamma gene mutations H655Y identify include L452R Lineage Local melting melting curve analysis monitoring Multiplex PCR N501Y nucleic acid testing P681R pangolin Pangolin lineage PCR performed polymerase chain reaction polymorphism positive sample S-gene mutation S-gene mutations SARS-CoV-2 SARS-CoV-2 lineage SARS-CoV-2 viral RNA Sequencing Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Severe acute respiratory syndrome coronavirus 2 variants single nucleotide Single nucleotide polymorphism SNP SNPs target Testing Testing algorithm the SARS-CoV-2 variant variants Viral RNA was performed WGS whole-genome sequencing [DOI] 10.1016/j.cmi.2021.09.007 PMC 바로가기 [Article Type] Article
Performance of a high-throughput, automated enzyme immunoassay for the detection of SARS-CoV-2 antigen, including in viral “variants of concern”: Implications for clinical use바이러스 "관심 있는 변이체"를 포함하여 SARS-CoV-2 항원 검출을 위한 고처리량 자동화 효소 면역분석의 성능: 임상 사용에 대한 시사점Article Published on 2022-01-012022-09-11 Journal: Journal of Clinical Virology [Category] SARS, 변종, 치료기술, [키워드] alternative Antigen antigen test automated Clinical use Cohort cohorts of patients collected complement diagnostic Direct EIA enzyme enzyme immunoassay Epidemic epidemiological evaluated French immunoassay in viral Laboratory NPs NPSs nucleic acid nucleic acid testing Patient performance positive provided reported retrospective cohort RT-PCR SARS CoV SARS CoV-2 SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 RNA SARS-CoV-2 viral second wave sensitivities sensitivity specificity subject subjects Support tested thresholds variant variants variants of concern viral antigens [DOI] 10.1016/j.jcv.2021.105048 PMC 바로가기 [Article Type] Article
Smaller reaction volume of triplex taqman real‐time reverse transcription‐PCR assays for diagnosing coronavirus disease 2019Research Article Published on 2021-12-032023-06-30 Journal: Journal of Clinical Laboratory Analysis [Category] SARS, [키워드] COVID‐19 nucleic acid testing RT‐PCR SARS‐CoV‐2 [DOI] 10.1002/jcla.24137 PMC 바로가기 [Article Type] Research Article