High sensitivity SARS-CoV-2 detection using graphene oxide-multiplex qPCRArticle Published on 2022-11-222022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome amplification applied coronavirus detect detection diagnoses gold standard Graphene Graphene oxide high sensitivity limit of detection molecular multiplex Multiplex qPCR pandemic pathogen detection polymerase chain primer qPCR qPCR assay reduce reference gene RNase P RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 gene SARS-CoV-2. sensitivity single-stranded DNA ssDNA target virus [DOI] 10.1016/j.aca.2022.340533 PMC 바로가기
Tracking SARS-CoV-2 in rivers as a tool for epidemiological surveillanceArticle Published on 2022-11-202022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] activities analysis Bacteria Caldera can be used characterized collected Concentration Contamination contributed correlated correlation COVID-19 COVID-19 cases discharge epidemiological epidemiological situation epidemiological surveillance evaluate fecal health authorities Human Human tracers impacted limit microbiologically negatively affected normalization normalized not affected plant quantified receiving reflected reported river Rivers impacted by wastewater RNase P RT-qPCR Salta SARS-CoV-2 SARS-CoV-2 surveillance significant increase similarity Support Surveillance target Viral concentration water quality water quality. [DOI] 10.1016/j.scitotenv.2022.157707 PMC 바로가기
Adsorption of SARS-CoV-2 onto granular activated carbon (GAC) in wastewater: Implications for improvements in passive samplingArticle Published on 2022-11-152022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, [키워드] activated acute respiratory syndrome Adsorption Adsorption capability anticipated approaches calculated Characteristics Concentration coronavirus DI water experiment feasible granular Granular activated carbon hybrid improvement interval Laboratory limitations occurred offer Passive Passive samplers potential benefit reached reproducibility RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA sensitivity Surveillance viral pathogen virus wastewater Wastewater surveillance. [DOI] 10.1016/j.scitotenv.2022.157548 PMC 바로가기
Wastewater surveillance allows early detection of SARS-CoV-2 omicron in North Rhine-Westphalia, GermanyArticle Published on 2022-11-102022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] Analysis approach close monitoring collected COVID-19 COVID-19 surveillance demonstrated detection Detection workflow dominant variant Epidemiology evaluate fraction Germany identify Initially K417N Laboratory Local MONITOR mutant Mutation N501Y NGS North omicron Omicron, B.1.1.529 PCR primer public health purified Regulation required resulting RT-qPCR SARS-CoV-2 SARS-CoV-2 epidemiology SARS-CoV-2 monitoring SARS-CoV-2 RNA SARS-CoV-2 variant Sequencing state subsequent Support Surveillance Treatment variant variant of concern was performed wastewater Wastewater-based epidemiology (WBE). [DOI] 10.1016/j.scitotenv.2022.157375 PMC 바로가기
One-step colorimetric isothermal detection of COVID-19 with AI-assisted automated result analysis: A platform model for future emerging point-of-care RNA/DNA disease diagnosisResearch article Published on 2022-11-012022-10-05 Journal: Talanta [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] Accuracy Analysis analyzed approach automated Colorimetric RT-LAMP Complete Concordance COVID-19 COVID-19 testing cycle threshold Detection limit diagnosis of COVID-19 Disease diagnosis DNA downstream ENhance exhibited faster feasibility Interpretation isothermal LAMP-DETR limit machine learning majority observation offer pandemic pathogen performed Perspective platform point-of-care RT-LAMP RT-qPCR SARS-CoV-2 sensitivity specificity [DOI] 10.1016/j.talanta.2022.123375 [Article Type] Research article
Evaluation of the impact of pre-analytical conditions on sample stability for the detection of SARS-CoV-2 RNAArticle Published on 2022-11-012022-11-15 Journal: Journal of virological methods [Category] SARS, 치료기술, 치료제, [키워드] collected Community condition conditions cycle threshold Demand Diagnosis diagnostics False negative increase in independent Laboratory MOST PBS postal service provide RNA RNA extraction RT-qPCR Sample Sample stability. SARS-CoV-2 SARS-CoV-2 E gene SARS-CoV-2 RNA sensitivity stability Swab temperature tested Transport media transported Variation Viral load VTM [DOI] 10.1016/j.jviromet.2022.114607 PMC 바로가기
Fate of SARS-CoV-2 coronavirus in wastewater treatment sludge during storage and thermophilic anaerobic digestionArticle Published on 2022-11-012022-11-15 Journal: Environmental research [Category] SARS, 치료기술, [키워드] addition ambient anaerobic Bovine coronavirus conducted COVID-19 outbreak Detection limit digestion droplet droplet digital PCR Final inactivated knowledge Laboratory Max 6) other coronaviruses Particle PCR question raised reported RNA RT-qPCR SARS-CoV-2 SARS-CoV-2 coronavirus SARS-CoV-2 detection SARS-CoV-2 particle SARS-CoV-2 particles SARS-CoV-2 stability Sludge Stability. Stool stools temperature Treatment virus were used [DOI] 10.1016/j.envres.2022.114057 PMC 바로가기
Pandemic driven innovation: A pilot evaluation of an alternative respiratory pathogen collection deviceArticle Published on 2022-11-012022-11-16 Journal: The American journal of emergency medicine [Category] SARS, 유전자 메커니즘, [키워드] absence All participant amplification communicable disease COVID-19 demonstrated determine diagnostic enrolled evaluated faster favor feedback fused gold standard identify inhibitor Innovation Interpretation invasive mechanism nasopharyngeal Nasopharyngeal swab nucleic acids participant Patient PCR PCR cycle PCR testing pilot study polymerase chain positive producing real-time reverse transcriptase receiving respiratory respiratory pathogen respiratory virus Result resulting RNase P RNAseP RT-qPCR Self-collection respiratory pathogen device. specimen study participant TaqPath tested threshold [DOI] 10.1016/j.ajem.2022.08.047 PMC 바로가기
SARS-CoV-2 infection and replication kinetics in different human cell types: The role of autophagy, cellular metabolism and ACE2 expressionArticle Published on 2022-11-012022-11-16 Journal: Life sciences [Category] SARS, 유전자 메커니즘, [키워드] A549 ACE2 ACE2 expression angiotensin ATP synthase autophagy BEAS-2B Brain Caco-2 can be used Cell cell line cell lines cellular cellular metabolism COVID-19 COX endothelial energy metabolism evaluated expression level HEK-293 human cell human cell line in vitro intestine intrinsically investigated kidney liver mechanism metabolism Mitochondria mitochondrial metabolism modified cell line molecular mechanism mRNA expression NDUFS2 overexpressed overexpressing overexpression Protein Replication replication kinetics respiratory tract RT-qPCR SARS-COV-2 infection SARS-CoV-2 replication SH-SY5Y susceptibility susceptible cell TMPRSS2 TMPRSS2 expression TMPRSS2. uninfected cell various tissue viral infection Viral load viral replication were assessed western blot [DOI] 10.1016/j.lfs.2022.120930 PMC 바로가기
A precise review on NAATs-based diagnostic assays for COVID-19: A motion in fast POC molecular testsArticle Published on 2022-11-012022-11-15 Journal: European Journal of Clinical Investigation [Category] SARS, 치료기술, [키워드] addressed advance approved assist authorization Care Comparative coronavirus disease COVID-19 diagnostic Diagnostic method disease Emergency expand false-negative global pandemic Health limitation mitigate molecular molecular diagnostic assays molecular test NAAT New nucleic acid amplification nucleic acid amplification techniques parameter platform POC POC testing point-of-care Point-of-care testing. polymerase chain RT-qPCR SARS-CoV-2 Sensitivity and specificity suggested [DOI] 10.1111/eci.13853 PMC 바로가기