Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) ProteinArticle Published on 2022-01-272022-10-31 Journal: Cells [Category] COVID-19, [키워드] Cryptococcus neoformans activate biochemical cleavage site comparable cryptococcal infection Cryptococcus Efficiency fluorogenic peptide furin Infection peptide protease Protein proteolytic cleavage PRRA S protein S1 and S2 S1/S2 cleavage site S1/S2 site SARS-CoV-2 SARS-CoV-2 disease sequence spike spike (S) protein the SARS-CoV-2 unique [DOI] 10.3390/cells11030437 PMC 바로가기 [Article Type] Article
CD4+ T Cell Immune Specificity Changes After Vaccination in Healthy And COVID-19 Convalescent Subjects건강하고 COVID-19 회복기 대상에서 백신 접종 후 CD4+ T 세포 면역 특이성 변화Immunology Published on 2022-01-192022-09-12 Journal: Frontiers in Immunology [Category] 진단, 치료기술, [키워드] Activation adaptive Adaptive immunity administration analyzed Anti-RBD IgG anti-S Antibody Response BNT162b2 vaccine CD4+ CD4+ T cell change changes in contrary convalescent individual correlation COVID-19 detectable develop elicit first dose healthy donor IgA IgA titer Immune profile immune response Immunity increase in lymphocyte Patient peptide positive positive individuals pre-vaccination promoted reactivity S1 and S2 SARS-CoV-2 vaccination second dose second vaccination T cell vaccination Vaccine vaccine administration [DOI] 10.3389/fimmu.2021.755891 PMC 바로가기 [Article Type] Immunology
SARS-CoV-2 vaccines elicit durable immune responses in infant rhesus macaquesSARS-CoV-2 백신은 유아 붉은털 원숭이에서 지속적인 면역 반응을 이끌어냅니다.Article Published on 2022-01-132022-09-12 Journal: Science Immunology [Category] MERS, SARS, 진단, [키워드] ACE2 Adults adverse effects antibody binding blocking activity cellular response Cellular responses COVID-19 decrease domain eight elicit elicited group Health IFN-γ IgG IL-17 immune function immune response immune responses Immunity immunized immunogenic immunogenicity Impact Inclusion Infant Infants intramuscularly limit Lipid nanoparticle Lipid nanoparticles magnitude memory B cell memory B cells mitigate Moderna mRNA-1273 vaccine mRNA neutralizing antibody titers novel vaccines peaking pediatric prefusion Prevent proof proof of concept Protein purified RBD rhesus macaque S protein S-2P S1 and S2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 vaccine SARS-CoV-2 vaccines severe complication severe complications squalene T cell T cell response the SARS-CoV-2 TLR7 TLR7/8 TNF-α Transmission transmission of SARS-CoV-2 two groups Vaccine [DOI] 10.1126/sciimmunol.abj3684 PMC 바로가기 [Article Type] Article
Generation of Spike-Extracellular Vesicles (S-EVs) as a Tool to Mimic SARS-CoV-2 Interaction with Host CellsSARS-CoV-2와 숙주 세포의 상호 작용을 모방하는 도구로 스파이크-세포외 소포(S-EV) 생성Article Published on 2022-01-032022-09-12 Journal: Cells [Category] SARS, 진단, [키워드] Analysis anti-ACE2 antibody biogenesis bronchial epithelial cell Cell cells characterized clinical trials co-expressed coding Colchicine collected COVID-19 drugs embryonic kidney endothelial EVs expressing Extracellular vesicles generate Generation host-virus interaction internalization mechanism Microscopy MIMIC non-transfected reduced S1 and S2 Safe SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein significantly spike Spike protein subunits the SARS-CoV-2 therapeutic Vesicle virus [DOI] 10.3390/cells11010146 PMC 바로가기 [Article Type] Article
Humoral response to spike S1 and S2 and nucleocapsid proteins on microarray after SARS-CoV-2 infectionSARS-CoV-2 감염 후 마이크로어레이의 스파이크 S1 및 S2 및 뉴클레오캡시드 단백질에 대한 체액 반응Article Published on 2022-01-012022-09-11 Journal: Journal of medical virology [Category] MERS, SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Anti-S1 anti-S2 antibody Antibody avidity Antibody Response Antigen Asymptomatic clinical evolution Comorbidities coronavirus coronavirus disease Coronavirus disease 2019 coronavirus disease 2019) COVID-19 Deceased disease severity dynamic evaluated functional groups humoral immune response Humoral response IgA IgG IgG antibodies IgG response median Microarray Mild moderate natural infection not differ nucleocapsid nucleocapsid protein Nucleocapsid proteins observation positive correlation presence of comorbidities Protein quantification respiratory response S1 and S2 SARS-CoV-2 SARS-COV-2 infection Seroconversion seroreversion serum sample serum samples severe acute respiratory syndrome Coronavirus severe coronavirus disease significantly spike subunit subunits Test ViraChip® Viral Viral RNA Volunteer volunteers [DOI] 10.1002/jmv.27290 PMC 바로가기 [Article Type] Article
Site-Specific Glycosylation Patterns of the SARS-CoV-2 Spike Protein Derived From Recombinant Protein and Viral WA1 and D614G StrainsChemistry Published on 2021-11-192022-10-31 Journal: Frontiers in Chemistry [Category] COVID-19, [키워드] ACE2 receptor Analysis analyzed Cell cell substrate characterized complex core Critical D614G D614G variant defined domain downstream Eclipse Efficiency glycan glycan shield glycans glycosylated glycosylation glycosylation pattern HEK293 high-mannose Host Host–pathogen interaction hydrophilic in viral Infection Interaction less Local microheterogeneity monomer Mutation N-glycan N-glycosylation N-glycosylation site O-glycosylation Orbitrap pattern predicted produced Protein RBD recent recombinant Recombinant protein reported reveal S1 and S2 S1 domain SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein spike Spike protein Spike proteins strain substrate the SARS-CoV-2 Vero Vero cells Vero E6 virus WA1 WA1 strain [DOI] 10.3389/fchem.2021.767448 PMC 바로가기 [Article Type] Chemistry
Assessment of Anti-SARS-CoV-2 Antibodies Post-Coronavac Vaccination in the Amazon Region of BrazilCommunication Published on 2021-10-122022-10-28 Journal: Vaccines [Category] COVID-19, [키워드] 95% CI age anti-SARS-CoV-2 antibody antibody reactivity assessment Brazil CoronaVac COVID-19 cross-sectional distribution dose Effectiveness evaluated Frequency greater group humoral immune response immune system in both groups individual Neutralizing Neutralizing antibodies neutralizing antibody Older Prevalence Receptor binding Region S1 and S2 SARS-CoV-2 Seropositivity significantly tested total antibody two groups Vaccine Volunteer [DOI] 10.3390/vaccines9101169 PMC 바로가기 [Article Type] Communication
Perceptions and knowledge of telemedicine in Ecuadorian practicing physicians: an instrument adaptation, validation and translation from English to SpanishResearch Published on 2021-10-022022-10-28 Journal: BMC Public Health [Category] Coronavirus, MERS, SARS, [키워드] Analysis Care changes in collected comparative fit index Consensus country COVID-19 pandemic criteria Cronbach’s alpha cross disease domain domains EFA English evaluate Exploratory factor analysis facilitate Factor Factors groups healthcare healthcare professionals internal consistency Isolation knowledge Likert scale measure Measures Non-COVID-19 Pathologies Patient care Perception perceptions physician practitioners Prevent quarantine question questionnaire random reducing reliability Research Result risk S1 and S2 selected separated similarity Spanish Spread supplementary material Telemedicine tested translated translation was measured was performed [DOI] 10.1186/s12889-021-11826-1 PMC 바로가기 [Article Type] Research
Recombinant Protein Expression and Purification of N, S1, and RBD of SARS-CoV-2 from Mammalian Cells and Their Potential ApplicationsArticle Published on 2021-09-302022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] acute respiratory syndrome Antigen application cells cleaved coronavirus coronavirus disease country COVID-19 demonstrated diagnostic diagnostics enzyme-linked immunosorbent assay expression human serum identify immunogenic induce infected with COVID-19 mammalian N protein Neutralizing antibodies nucleocapsid pandemic Patient plasmids Potential Protein purified RBD RBD of SARS-CoV-2 RBD proteins reached Receptor binding domain recombinant S1 and S2 SARS-CoV-2 serological Serological assay severe acute respiratory syndrome coronavirus 2 Spike protein structural protein the receptor-binding domain the S1 subunit the SARS-CoV-2 Viral antigen was used [DOI] 10.3390/diagnostics11101808 PMC 바로가기 [Article Type] Article
Targeting novel coronavirus SARS-CoV-2 spike protein with phytoconstituents of Momordica charantiaMomordica Charantia의 식물성 분열제를 갖는 소설 코로나 바이러스 SARS-COV-2 스파이크 단백질 표적Research Published on 2021-09-272022-08-31 Journal: Journal of Ovarian Research [Category] SARS, 변종, 치료제, [키워드] active site ADME Affect binding binding affinity binding energy Bitter Bitter melon component Compound COVID-19 docking Docking studies docking study domain drug effort Erythrodiol evaluate hydrophilic hydrophobic hydrophobic interactions identify in-silico infection rate Interaction Melon Momordica Momordica Charantia novel coronavirus SARS-CoV-2 PyMol reduce Result reveal S1 and S2 S1 and S2 domains SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2 viral infection SARS-CoV-2 virus selection pressure Spike protein Spike proteins SPR strong interactions surface plasmon surface plasmon resonance targeting tested the SARS-CoV-2 the SARS-CoV-2 virus the spike protein treat vaccination variant Viral viral infections was used were used [DOI] 10.1186/s13048-021-00872-3 PMC 바로가기 [Article Type] Research