Sensitivity of SARS-CoV-2 Life Cycle to IFN Effects and ACE2 Binding Unveiled with a Stochastic ModelArticle Published on 2022-02-152022-10-28 Journal: Viruses [Category] COVID-19, COVID19(2023년), Fulltext, SARS, [키워드] ACE2 ACE2 binding antiviral treatments binding caused Cell chain Characteristics component cycle deterministic model Effect fluctuation help IFN Infection infection process inhibitory effect life Markov Markov chain Monte Carlo method mathematical mathematical model Model parameter Probability progeny SARS-CoV-2 sensitivity analysis Single Cell statistical stochastic stochastic processes the ACE2 receptor the SARS-CoV-2 to define turn Type I IFN type I interferon (IFN) Variation virus dynamics [DOI] 10.3390/v14020403 PMC 바로가기 [Article Type] Article
Cell specific peripheral immune responses predict survival in critical COVID-19 patients세포 특이적 말초 면역 반응은 중요한 COVID-19 환자의 생존을 예측합니다Article Published on 2022-02-152022-09-10 Journal: Nature Communications [Category] COVID19(2023년), MERS, SARS, 유전자 메커니즘, [키워드] Accuracy Activation antibody Antigen presentation Applied immunology Blood CD14 CD16 Cell cell cycle cell type cell types cells circulating Clinical management complex COVID-19 COVID-19 patients Critical critical COVID-19 patient critical COVID-19 patients Critically ill critically ill COVID-19 patients dataset Differential gene expression framework identify identifying Immune cell immune cell type independent Infection interferon interferon signaling machine mechanism monocyte Monocytes mononuclear cells Mortality NK-cell Pathogens pathway Peripheral blood peripheral immune response predict predictor prognostic indicator promote Regulation risk stratification SARS-CoV-2 sequence Single Cell single cell sequencing Single-cell RNA sequencing survival systemic immune response transcriptional transcriptomes transcriptomics Trigger triggers viral infection [DOI] 10.1038/s41467-022-28505-3 PMC 바로가기 [Article Type] Article
Subcellular Detection of SARS-CoV-2 RNA in Human Tissue Reveals Distinct Localization in Alveolar Type 2 Pneumocytes and Alveolar MacrophagesResearch Article Published on 2022-02-082022-10-29 Journal: mBio [Category] COVID-19, MERS, [키워드] AGER alveolar Alveolar macrophages amplification AT1 AT2 Autopsy caused Cell Cell culture cell line Cellular imaging coronavirus disease COVID-19 detection diagnostics Drug screening FFPE fixation fluorescent image analysis formalin Human human lung tissue human tissue identify infected patients infected with SARS-CoV-2 Infection Localization lung Lymph node macrophage marker Marco marker gene marker genes novel coronavirus SARS-CoV-2 observé ORF1a paraffin embedding pathology performed Placenta platform pneumocyte probe Region regions RNA RNA FISH SARS-CoV-2 SARS-CoV-2 RNA SFTPC Single Cell specimens subcellular subcellular localization subgenomic mRNAs tissue tissues Type viral life cycle Viral RNA widespread [DOI] 10.1128/mbio.03751-21 PMC 바로가기 [Article Type] Research Article
Myeloid cell interferon responses correlate with clearance of SARS-CoV-2골수 세포 인터페론 반응은 SARS-CoV-2 제거와 관련이 있습니다Article Published on 2022-02-032022-09-10 Journal: Nature Communications [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료제, [키워드] accumulation ACE2 angiotensin angiotensin-converting enzyme 2 Anti-viral appearance BAL baseline Bronchoalveolar lavage CD163 CD68 Cell cell suspension changes in clearance of SARS-CoV-2 correlated COVID-19 patients COVID-19-related death dendritic cells emergence exhibiting expressing expression gene signature heterogeneous HLADR host response host responses Human immune Immune cell immune profiling immunopathology increased risk individual cells infected with SARS-CoV-2 Infection Inflammation Inflammatory Innate immunity interferon interferon response lung Lungs macaque macaques Macrophage macrophages Mutant SARS-CoV-2 myeloid observé plasmacytoid dendritic cell plasmacytoid dendritic cells Population reported response rhesus macaque rhesus macaques RNAseq SARS-CoV-2 SARS-COV-2 infection scRNAseq SIGLEC1 significantly Single Cell strain T-lymphocyte T-lymphocytes transcriptional transcriptional change Transcriptional changes Type type I interferon type I interferon response Viral viral clearance viral dynamics viral infection Viremia while [DOI] 10.1038/s41467-022-28315-7 PMC 바로가기 [Article Type] Article
Promising Assays for Examining a Putative Role of Ribosomal Heterogeneity in COVID-19 Susceptibility and SeverityReview Published on 2022-01-282022-10-29 Journal: International Review of Cell and Molecular Biology [Category] COVID-19, [키워드] analyses Antiviral treatment arise assays for proteins assays for RNA sequence and structure caused Characteristics characterized clinical sample clinical setting component concurrent COVID-19 COVID-19 disease Critical detect droplet Efficacy element environmental cues Examining generate heterogeneity Host host susceptibility identify include Infection Messenger RNA modifications mRNA Nanopore organism post-translational modification protocol responses ribosomal heterogeneity ribosomal protein ribosomal proteins ribosomal RNA ribosomal RNAs ribosome ribosomes RNA genome RNA sequencing role rRNA rRNAs SARS-CoV-2 SARS-CoV-2 coronavirus selected sequence Sequencing severity of symptoms Side effect Single Cell Statistical power structural variations structures substrate Symptoms the disease the SARS-CoV-2 transcript translate translation translation machinery translational Transmissibility unique vaccine immunogenicity vaccine response variant viral infectivity [DOI] 10.3390/life12020203 PMC 바로가기 [Article Type] Review
SCovid: single-cell atlases for exposing molecular characteristics of COVID-19 across 10 human tissuesSCovid: 10개의 인간 조직에서 COVID-19의 분자 특성을 노출하기 위한 단일 세포 지도책AcademicSubjects/SCI00010 Published on 2022-01-072022-09-10 Journal: Nucleic Acids Research [Category] 유전자 메커니즘, 진단, [키워드] accompanied acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Analysis carried caused cell types Characteristics collected comprehensive resource coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 dataset datasets DEGs differentially expressed gene Epidemic expressed gene human tissue human tissues independent information molecular multiple-organ failure provide respiratory SARS-CoV-2 severe acute respiratory syndrome Coronavirus Single Cell single cells single-cell single-cell dataset tissues virus [DOI] 10.1093/nar/gkab881 PMC 바로가기 [Article Type] AcademicSubjects/SCI00010
Delayed induction of type I and III interferons mediates nasal epithelial cell permissiveness to SARS-CoV-2Article Published on 2021-12-072022-10-04 Journal: Nature Communications [Category] SARS, 임상, 진단, [키워드] Air-liquid interface airway Antiviral Cell change Critical Culture Delayed epithelial epithelial cell epithelial cell types host response IFN IFN response IFNβ induce Infection initiate innate immune response Innate immunity Interaction interferon interferon-stimulated gene interferons Mucosal immunology nasal nasal epithelium outcome paracrine Pathogenesis proteomics provided recombinant IFN response restrict SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication SARS-CoV-2 viral replication Single Cell Single-cell RNA sequencing tissue Transcription Transmission type I viral gene expression widespread [DOI] 10.1038/s41467-021-27318-0 PMC 바로가기 [Article Type] Article
Clonal Hematopoiesis of Indeterminate Potential Is Associated with Increased Age-Independent Morbidity and Mortality in Patients with COVID-19- the Beyond DNA COVID-19 ProjectConference abstract Published on 2021-11-242022-10-05 Journal: Blood [Category] 변종, [키워드] accounted affected age age and sex allele approved assays association ASXL1 ATAC-seq baseline Blood blueprint carried ChIP clinically collected common Comorbidities comparable Coverage COVID-19 COVID-19 diagnosis COVID-19 disease severity COVID-19 patient CRP CRS cytokine cytokine levels Cytokine release syndrome Cytokines database death defined described Detection limit DNA download elevated ELISA assay entire cohort figure Fisher Follow-up funding Genes grade 3 group had more hazard healthy individual Hematopoiesis Hospitalization hypoxemic hypoxic respiratory failure identify IL6 Increased increased mortality indeterminate indicated individuals Infection Inflammation Inflammatory inflammatory cascade Inflammatory marker IRB male Mayo Clinic MCP-1 median median age mononuclear cell morbidity Mortality MPL Mutation mutations neutropenia no difference observé Older pathophysiology Patient patients patients with COVID-19 PBMC Peripheral blood Potential proteomic study reached regression models remained Research resolved Respiratory failure RNA scale Sequencing severe disease severity Sex SF3B1 Single Cell ssDNA subsequent survival survival analysis TET2 the Kaplan-Meier method the WHO TNF-a TP53 two groups variables variant was performed WHO Wilcoxon rank sum with COVID-19 [DOI] 10.1182/blood-2021-151548 [Article Type] Conference abstract
COVID‐19 and vertical transmission: assessing the expression of ACE2/TMPRSS2 in the human fetus and placenta to assess the risk of SARS‐CoV‐2 infectionResearch Article Published on 2021-11-182022-10-28 Journal: BJOG : an international journal of obstetrics and [Category] COVID-19, MERS, SARS, [키워드] Abstract ACE2 Adults amniotic analysed Analysis children concerning COVID‐19 COVID‐19 infection COVID‐19 dataset epithelial explain expressed expression fetal fetal infection finding gastrointestinal tract help immunohistochemistry indicate Infection intestine kidney lung performed Placenta presenting Protein protein expression protein level Proteins provide Repository required Retrospective analysis risk SARS‐CoV‐2 infection SARS‑CoV2 SARS‐COV2 scRNAseq significant level Single Cell susceptibility susceptible tissue tissues TMPRSS2 Transmission unlikely vertical transmission virus women [DOI] 10.1111/1471-0528.16974 PMC 바로가기 [Article Type] Research Article
Cross-Neutralization of Emerging SARS-CoV-2 Variants of Concern by Antibodies Targeting Distinct Epitopes on Spike스파이크의 독특한 에피토프를 표적으로 하는 항체에 의한 우려의 신흥 SARS-CoV-2 변이체의 교차 중화Clinical Trial Published on 2021-11-162022-09-10 Journal: mBio [Category] MERS, SARS, 변종, 비임상, 임상, 진단, [키워드] (Alpha) acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 affected Alpha antibody B.1.1.7 B.1.617.2 binding breath concern convalescent coronavirus Critical disrupt distinct domain Donor epitope Epitopes Human Humoral immunity immune memory Immunity induce Infectious disease K417 mAb mAbs monoclonal antibodies monoclonal antibody Mutation natural infection neutralize Neutralizing Neutralizing antibodies neutralizing antibody P.1 pandemic prototype RBD Receptor binding domain Region respiratory SARS-CoV-2 SARS-CoV-2 spike Single Cell spike Spike protein target targeting the SARS-CoV-2 the spike protein Transmissibility vaccination variant variants variants of concern Viral Viral variants VOCs [DOI] 10.1128/mBio.02975-21 PMC 바로가기 [Article Type] Clinical Trial