Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2 in patients with COVID-19Article Published on 2022-01-012023-06-19 Journal: Clinical microbiology and infection : the official [Category] MERS, SARS, [키워드] COVID-19 Infective viral shedding SARS CoV-2 subgenomic RNA virus culture [DOI] 10.1016/j.cmi.2021.08.009 PMC 바로가기
Viable Severe Acute Respiratory Syndrome Coronavirus 2 Isolates Exhibit Higher Correlation With Rapid Antigen Assays Than Subgenomic RNA or Genomic RNAMicrobiology Published on 2021-11-122022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] acute respiratory syndrome Ag-RDT Ag-RDTs analytical sensitivity antigen-detection assays Cell Cell culture cohort study coronavirus coronavirus 2 COVID-19 Culture cycle threshold detect Detection sensitivity effective evaluated exhibited genomic genomic RNA gold immunoassay individuals Infection Infectivity isolate Isolation log negative controls Patient patients with SARS-CoV-2 PCR performed post-symptom onset Rapid Rapid diagnostic test respiratory respiratory sample RNA rRT-PCR SARS-CoV-2 sensitivity specificity Spread subgenomic RNA symptom onset tested Viable [DOI] 10.3389/fmicb.2021.718497 PMC 바로가기 [Article Type] Microbiology
Nucleic Acid-Based Treatments Against COVID-19: Potential Efficacy of Aptamers and siRNAsMicrobiology Published on 2021-11-082022-10-31 Journal: Frontiers in Microbiology [Category] COVID-19, [키워드] acting Against antiviral therapy approach approved aptamer aptamer–siRNA chimera candidate cleave COVID-19 disease drug effective Effectiveness genomic Infection inhibit limitations Pathogenesis Potential reduce Replication RNA interference siRNA siRNAs Stage subgenomic RNA the disease the SARS-CoV-2 these compound Treatment treatments for COVID-19 viral infections viral life cycle viral loads Virus neutralization virus-neutralizing [DOI] 10.3389/fmicb.2021.758948 PMC 바로가기 [Article Type] Microbiology
Correlation of SARS-CoV-2 Subgenomic RNA with Antigen Detection in Nasal Midturbinate Swab Specimens비강 중비갑개 면봉 표본에서 항원 검출과 SARS-CoV-2 서브게놈 RNA의 상관관계Expedited Published on 2021-11-012022-09-10 Journal: Emerging Infectious Diseases [Category] MERS, SARS, 치료기술, 치료법, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Antigen antigen detection coronavirus coronavirus disease COVID-19 detectable detection diagnostics identify nasal nasal midturbinate nasopharyngeal Nasopharyngeal swab nasopharyngeal swab specimen Nasopharyngeal swab specimens nucleocapsid outpatients remained respiratory respiratory infections RNA SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 subgenomic RNA swab specimen swab specimens symptomatic virus replication viruses zoonoses [DOI] 10.3201/eid2711.211135 PMC 바로가기 [Article Type] Expedited
Severe Acute Respiratory Syndrome Coronavirus 2 Total and Subgenomic RNA Viral Load in Hospitalized PatientsArticle Published on 2021-10-282023-06-19 Journal: The Journal of Infectious Diseases [Category] MERS, SARS, [키워드] RT-qPCR SARS-CoV-2 subgenomic RNA [DOI] 10.1093/infdis/jiab215 PMC 바로가기
Severe Acute Respiratory Syndrome Coronavirus 2 Normalized Viral Loads and Subgenomic RNA Detection as Tools for Improving Clinical Decision Making and Work ReincorporationObservational Study Published on 2021-10-282023-06-19 Journal: The Journal of Infectious Diseases [Category] MERS, SARS, [키워드] COVID-19 Healthcare workers normalized viral loads SARS-CoV-2 subgenomic RNA [DOI] 10.1093/infdis/jiab394 PMC 바로가기 [Article Type] Observational Study
Dynamics of Viral Shedding and Symptoms in Patients with Asymptomatic or Mild COVID-19무증상 또는 가벼운 Covid-19 환자의 바이러스 쉐딩 및 증상의 역학Article Published on 2021-10-222022-09-10 Journal: Viruses [Category] MERS, SARS, 치료기술, [키워드] Asymptomatic asymptomatic patient asymptomatic patients Cell culture change Community conducted Course COVID-19 COVID-19 adult patient COVID-19 patient decrease Diagnosis disease course Dynamics early disease enrolled exhibited genomic RNA in viral individual Isolation loss of infectivity Mild presymptomatic prospective cohort study RNA Saliva saliva samples SARS-CoV-2 shedding subgenomic RNA Symptom symptom onset symptomatic symptomatic patient Symptomatic patients the disease viable culture Viral Viral load viral shedding virus were measured [DOI] 10.3390/v13112133 PMC 바로가기 [Article Type] Article
Tracking the Transcription Kinetic of SARS-CoV-2 in Human Cells by Reverse Transcription-Droplet Digital PCRArticle Published on 2021-10-022022-10-28 Journal: Pathogens [Category] COVID-19, [키워드] Caco-2 cells Cell contribute coronavirus COVID-19 patients detect develop Digital Digital droplet PCR drug help Human human cells Infection Invasion mechanism ORF PCR Postinfection Remdesivir RNA SARS-CoV-2 SARS-COV-2 infection subgenomic RNA target target cells Therapeutic treatment Transcription transcriptional treat understanding viral gene viral RNA synthesis viral transcription virus replication [DOI] 10.3390/pathogens10101274 PMC 바로가기 [Article Type] Article
A Convenient and Biosafe Replicon with Accessory Genes of SARS-CoV-2 and Its Potential Application in Antiviral Drug DiscoverySARS-CoV-2의 보조 유전자와 항바이러스 약물 발견에 대한 잠재적 응용을 포함하는 편리하고 생물학적으로 안전한 복제품Article Published on 2021-10-012022-09-11 Journal: Virologica Sinica [Category] SARS, 치료제, [키워드] absence Accessory Analysis anti-SARS-CoV-2 antiviral drug Antiviral drug screening antiviral drugs application BAC Bacterial bacterial artificial chromosome benefit biosafety level Biosafety Level 3 BSL3 can be used cause cDNA Cell chromosome Containment costly Deletion disease drug effective drug effective drugs exonuclease expression genetics Genome genome replication Infection infectious inhibition assay Inhibition assays inhibitors Laboratory level 3 Live virus luciferase reporter gene methyltransferase Nsp12 nsp14 pandemic of COVID-19 pathogenicity Potential profile Replication replicon reporter gene Research research and development reverse genetics RNA RNA polymerase S protein SARS-CoV-2 sgRNA subgenomic RNA subgenomic RNAs this system transfection viral cDNA virion assembly virus wild-type virus [DOI] 10.1007/s12250-021-00385-9 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Subgenomic RNAs: Characterization, Utility, and PerspectivesSARS-COV-2 Subgenomic RNA : 특성화, 유용성 및 관점Review Published on 2021-09-242022-09-10 Journal: Viruses [Category] SARS, 유전자 메커니즘, [키워드] characterization Comprehensive COVID-19 COVID-19 pandemic etiologic agent marker Research RNA RNA genome root SARS-CoV-2 SARS-CoV-2 pathogenesis sgRNA sgRNAs subgenomic RNA subgenomic RNAs Viral viral replication [DOI] 10.3390/v13101923 PMC 바로가기 [Article Type] Review