Specific intracellular signature of SARS-CoV-2 infection using confocal Raman microscopy Research Published on 2022-07-252022-10-05 Journal: Communications Chemistry [Category] 바이오마커, 진단, [키워드] Accuracy amino-acid analyzed biochemical Biomarkers Cell contribute COVID-19 Data analysis demonstrated Factor identify infected cell Infection Interaction Microscopy Modification molecular non-infected Raman spectroscopy RNA virus RNA viruses SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2-infected cells significant increase Specific Spread target therapeutic intervention viral pathogenesis virus Virus-host interactions virus-infected cell [DOI] 10.1038/s42004-022-00702-7 [Article Type] Research
Reverse genetics systems for SARS-CoV-2SARS-CoV-2에 대한 역유전학 시스템Review Published on 2022-07-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antiviral drug BAC biosafety level Biosafety Level 2 BSL2 BSL3 caused characterization clone coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CPER feature functions genetics highlighting impair knowledge level 2 level 3 Limited mechanism mutant operation outbreak pandemic performed platform public health Research researcher researchers reverse genetics systems. SARS-CoV-2 severe acute respiratory syndrome Coronavirus TAR toolkit Viral particles viral pathogenesis Viral protein viral replication virus wild-type [DOI] 10.1002/jmv.27738 PMC 바로가기 [Article Type] Review
The 2022 outbreak and the pathobiology of the monkeypox virusArticle Published on 2022-07-012022-11-15 Journal: Journal of autoimmunity [Category] 진단, [키워드] addition African Antiviral antiviral therapies body fluids CDC center chills clade clades Congo basin Control disease effective Europe fatigue Fever headache Host hosts identity include individual Infection Infectious disease International lymph nodes mitigate Monkeypox Monkeypox outbreak 2022 muscle aches nasopharyngeal National Nigeria North America outbreak pandemic Pathogenesis Prevalence Prevent prevention raised replicate reported Respiratory droplets returned rodent shown specific treatment Spread Symptom The United States through contact Transmission transmitted Travel USA Vaccine viral pathogenesis virus virus infection virus spread Washington West while WHO World Health Organization [DOI] 10.1016/j.jaut.2022.102855 PMC 바로가기
SARS-CoV-2, SARS-CoV, and MERS-CoV encode circular RNAs of spliceosome-independent originSARS-CoV-2, SARS-CoV 및 MERS-CoV는 spliceosome-independent origin의 원형 RNA를 인코딩합니다.Article Published on 2022-07-012022-09-11 Journal: Journal of medical virology [Category] Coronavirus, COVID19(2023년), SARS, 유전자 메커니즘, 치료기술, 치료제, [키워드] abundance acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplified Analysis approaches assays Autoimmune disease Autoimmune diseases Betacoronavirus betacoronaviruses breakpoint canonical Chain Reaction characterized Circular circular RNA complementary coronavirus Critical cytoplasmic demonstrated distribution Features Fluorescence highlight homologous Host In situ hybridization infected Vero E6 cells junction mechanism MERS-CoV Middle East Middle East respiratory syndrome Coronavirus N gene not limited offer polymerase chain polymerase chain reaction predicted probe respiratory syndrome coronavirus RNA RNA Biology RNA genome RNA genomes RNA sample RNA samples RNA synthesis RNase SARS-CoV SARS-CoV-2 sequenced severe acute respiratory syndrome Coronavirus transcriptional regulatory sequences Transcriptome Vero E6 VERO E6 cells viral pathogenesis viral transcriptome Virology [DOI] 10.1002/jmv.27734 PMC 바로가기 [Article Type] Article
Nucleopore Traffic Is Hindered by SARS-CoV-2 ORF6 Protein to Efficiently Suppress IFN-β and IL-6 SecretionIFN-β 및 IL-6 분비를 효율적으로 억제하기 위해 SARS-CoV-2 ORF6 단백질에 의해 핵공 이동이 방해됨Article Published on 2022-06-112022-09-11 Journal: Viruses [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] Activation addition antagonistic antiviral response block cascade characterized clinical outcomes contributing to counteract Critical cytokine Cytokines cytoplasm cytoplasmic demonstrated ENCODE exacerbated Factors feature Genes Host host immune system IFN-β IL-6 immune immune evasion Infection Inflammation interferon interferon antagonist interleukin interleukin-6 MAVS MDA-5 mechanism Messenger RNA movement mRNA multifunctional NF-κB not affected novel SARS-CoV-2 virus nuclear translocation nucleus occurred ORF6 pro-inflammatory cytokine pro-inflammatory cytokines Protein reported RIG-I SARS-CoV-2 SARS-CoV-2 ORF6 protein SARS-CoV-2 virus Signaling STAT suppresse suppression the SARS-CoV-2 Traffic transcription factor transcription factors transcriptional viral infection viral pathogenesis virulence virus [DOI] 10.3390/v14061273 PMC 바로가기 [Article Type] Article
Computational study and design of effective siRNAs to silence structural proteins associated genes of Indian SARS-CoV-2 strains인도 SARS-CoV-2 균주의 구조 단백질 관련 유전자를 침묵시키는 효과적인 siRNA의 계산 연구 및 설계Article Published on 2022-06-012022-09-11 Journal: Computational biology and chemistry [Category] COVID19(2023년), SARS, 치료법, 치료제, [키워드] absence acute respiratory disease Ago2 Algorithm animal models antiviral drug antiviral drugs Antiviral treatment binding candidate collected complementary Consensus COVID-19 deaths docking Effect effective envelope protein feature free energy GC content gene silencing Genes Genome Health Organization heat capacity Human Human genome in vitro in vivo India melting temperature membrane protein molecular docking analysis nucleic acid nucleocapsid nucleotide nucleotides outbreak pandemic parameter pathogenic coronavirus pathway Post-transcriptional gene silencing Protein public health Region reported Respiratory disease RNA RNA interference RNAi SARS-CoV-2 SARS-CoV-2 strain SARS-CoV-2 strains selected sequence sequenced siRNA siRNA molecule siRNA molecules siRNAs structural protein structural proteins Surface glycoprotein target target gene target mRNA temperature Therapeutic treatment triggered viral pathogenesis WHO World Health Organization [DOI] 10.1016/j.compbiolchem.2022.107687 PMC 바로가기 [Article Type] Article
An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2SARS-CoV-2의 비오틴화된 상호작용자를 식별하기 위한 항체 기반 근접 라벨링 프로토콜Article Published on 2022-05-022022-09-12 Journal: STAR protocols [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] Analysis approach biotinylated Complete Host identify labeling mass mass spectrometry mechanism Microbiology molecular interaction Molecular/Chemical Probes non-specific peptide Protein Biochemistry Proteins proteomics protocol SARS-CoV-2 SARS-CoV-2 protein viral pathogenesis Viral proteins virus [DOI] 10.1016/j.xpro.2022.101406 PMC 바로가기 [Article Type] Article
Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts서브게놈 및 게놈 SARS-CoV-2 전사체의 수준을 측정하기 위한 새로운 RT-ddPCR 분석Article Published on 2022-05-012022-09-11 Journal: Methods (San Diego, Calif.) [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] arising clinical disease Clinical significance contribute coronavirus COVID-19 Digital digital PCR discontinuous transcription droplet droplet digital PCR expression genomic genomic RNA gRNA locus novel other coronavirus other coronaviruses positive-sense Quantitative assays recent Regulation Replication replication of SARS-CoV-2 reverse transcription RNA SARS-CoV-2 SARS-CoV-2 infected patient SARS-CoV-2 infected patients severity sgRNA sgRNAs subgenomic mRNA subgenomic mRNAs subgenomic RNA synthesis transcript Transcription transcripts viral pathogenesis viral transcription Viral transcription/replication. [DOI] 10.1016/j.ymeth.2021.04.011 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Point Mutation and Deletion Spectra and Their Association with Different Disease OutcomesSARS-CoV-2 점 돌연변이 및 삭제 스펙트럼과 다양한 질병 결과와의 연관성Article Published on 2022-04-272022-09-11 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, [키워드] Amino acid amino acid substitution amino acid substitutions Amplicon amplicons Analysis association average complexity consensus sequence coronavirus COVID-19 COVID-19 pandemic COVID-19 severity Deletion deletions develop diagnostic disease diversity index Effects functional Genetic variant genetic variants Haplotype infected patients isolate isolates location Mild mild COVID-19 symptoms Mild disease Mild symptom mild symptoms moderate moderate or severe disease mutant mutant spectra mutant spectrum Mutation mutations nasopharyngeal Nsp12 nsp12 (polymerase) Patient patients with moderate phenotypic Point Point mutation polymerase Region RNA RNA virus RNA viruses SARS-CoV-2 selective pressures Sequencing severe COVID-19 severe disease severity significantly significantly higher Spain spike three-dimensional structure ultradeep sequencing. viral pathogenesis virus [DOI] 10.1128/spectrum.00221-22 PMC 바로가기 [Article Type] Article
Glycosylation of viral proteins: Implication in virus–host interaction and virulenceArticle Published on 2022-04-182023-08-30 Journal: Virulence [Category] E형 간염, [키워드] glycosylation viral nonstructural protein viral pathogenesis viral structural protein Virus life cycle [DOI] 10.1080/21505594.2022.2060464 PMC 바로가기