Broadly targeted multiprobe QPCR for detection of coronaviruses: Coronavirus is common among mallard ducks ( Anas platyrhynchos )
Article
[키워드] C t, threshold cycle
amplified
Ana
avian coronavirus
competing
conserved
Coronaviruses
CoV
CoVs
decrease
detect
detection
duck
fatal disease
fecal samples
found
Frame
FRET, fluorescence resonance energy transfer
groups
host range
Human
LNA, locked nucleic acid
NQPCR, nuclease-based real-time reverse transcription PCR
Nuclease-based probes
ORF, open reading frame
PCR, polymerase chain reaction
Primers
probe
probes
provide
QPCR, quantitative real-time reverse transcription PCR
Real-time PCR
reduced
reservoirs
respiratory sample
reverse transcription PCR
RNA, Ribonucleic acid
RT-PCR, reverse transcriptase polymerase chain reaction
sensitivity
sequence
Split probe strategy
Spread
Strains
TaqMan
targets
TD, touch down temperature protocol
TET, tetrachloro-6-carboxyfluorescein
Transmission
variant
viral evolution
viral nucleic acid
[DOI] 10.1016/j.jviromet.2009.04.022 PMC 바로가기 [Article Type] Article
[DOI] 10.1016/j.jviromet.2009.04.022 PMC 바로가기 [Article Type] Article