Rapid detection of equine coronavirus by reverse transcription loop-mediated isothermal amplificationShort Communication Published on 2015-02-122022-11-01 Journal: Journal of virological methods [Category] Coronavirus, MERS, [키워드] addition analyzed assays collected conducted coronavirus cross-react Detection limit Diagnosis equine Equine coronavirus equipment Fecal sample greater Infection intestinal isothermal amplification Laboratory less outbreak pathogen performed polymerase chain qRT-PCR qRT-PCR assays Quantitative Quantitative RT-PCR Rapid real-time RT-PCR resources respiratory pathogen reverse transcription Reverse transcription loop-mediated isothermal amplification RNA RT-LAMP RT-PCR sensitivity Surveillance tested [DOI] 10.1016/j.jviromet.2015.02.001 PMC 바로가기 [Article Type] Short Communication
The Drosophila MAPK p38c Regulates Oxidative Stress and Lipid Homeostasis in the IntestineDrosophila MAPK p38c는 장의 산화 스트레스와 지질 항상성을 조절합니다Research Article Published on 2014-09-252022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] accumulate Analysis analyzed antimicrobial peptide Atf3 Bacterial infection cassette cellular Control digestive tract Drosophila ENCODE enzyme expressed expressing expression function gene encoding genetic analysis Genome Gut Human Immune homeostasis Immunity implicated induce Infection intestinal intestine involved kinase kinases Lipid lipid metabolism lipids MAPK Mekk1 metabolic genes metabolism midgut mutant mutants observation oxygen pathogen pathway peptide phenotype phosphorylate Phosphorylation physical Protein protein kinase protein kinases Pseudomona reactive oxygen species reduced regulate reveal ROS Signaling signaling pathway Specy Stress substrates susceptible Transcription transcription factor transcription factors up-regulated upregulated variety yeast [DOI] 10.1371/journal.pgen.1004659 PMC 바로가기 [Article Type] Research Article
An evaluation of contaminated complete feed as a vehicle for porcine epidemic diarrhea virus infection of naïve pigs following consumption via natural feeding behavior: proof of conceptResearch Article Published on 2014-08-052022-10-28 Journal: BMC veterinary research [Category] Coronavirus, MERS, [키워드] affected bioassay Clinical sign clinically Complete controls diarrhea Epidemic epidemic diarrhea experiment Feed in vivo Infection initial Initially intestinal lesion naïve negative negative control no evidence of PCR PEDV Porcine positive Result risk risk factor RNA Spread Support Transmission viral shedding virus virus infection vomiting was tested were used [DOI] 10.1186/s12917-014-0176-9 PMC 바로가기 [Article Type] Research Article
Sequence of a Complete Chicken BG Haplotype Shows Dynamic Expansion and Contraction of Two Gene Lineages with Particular Expression Patterns완전한 치킨 BG 일배체형의 시퀀스는 특정 발현 패턴을 가진 두 유전자 계통의 동적 확장 및 수축을 보여줍니다Research Article Published on 2014-06-052022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] Actin allelic Antigen presentation appear association Autoimmune disease Autoimmune diseases B12 brush border Cell cells change chicken chromosome chromosome 2 CNV complex contraction copy number Deletion domain dynamic Evolution expansion expressed expression family fixed Genes genetic association genetic associations Haplotype haplotypes homolog homologue homologues Human humans identify immune immune gene immune genes Immunity implicated Interaction intestinal involved major histocompatibility complex mammal mammals MHC multigene families multigene family myosin Particular pathogen Pathogens pattern polymorphism promoter promoters Recombination Region scrutiny sequence show single gene species tandem repeats the major histocompatibility tissue tissue expression tissues undergo untranslated regions Variation Viral diseases whole genome [DOI] 10.1371/journal.pgen.1004417 PMC 바로가기 [Article Type] Research Article
High-dose dietary zinc oxide mitigates infection with transmissible gastroenteritis virus in pigletsResearch Article Published on 2014-03-282022-10-28 Journal: BMC veterinary research [Category] Coronavirus, MERS, [키워드] added addition Apoptosis cause chloride Concentration control group coronavirus cytokine decrease detectable diarrhea Diseases down-regulation Electrophysiology epithelial evaluate the effect fecal Fecal consistency Final gastroenteritis group groups humoral immune response IFN in vivo incidence Infection interferon intestinal intestinal tract mechanism mitigate Morphometry mucosal immune OAS oral physiological PROTECT protective effect receiving reduce response Result secretion serum antibody response severity shown SLC30A1 SLC30A5 SLC39A4 stimulate TGEV three group transmissible gastroenteritis virus villus virus virus infection was performed Zinc zinc oxide Zinc transporters [DOI] 10.1186/1746-6148-10-75 PMC 바로가기 [Article Type] Research Article
Heterozygous De Novo and Inherited Mutations in the Smooth Muscle Actin ( ACTG2 ) Gene Underlie Megacystis-Microcolon-Intestinal Hypoperistalsis Syndrome평활근 액틴(ACTG2) 유전자의 이형 접합체 및 유전 돌연변이는 거대포낭-소결장-장 연동운동부전 증후군의 기초가 됩니다.Research Article Published on 2014-03-272022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] abdominal surgeries abdominal surgery ACTG2 Actin Actins affected affecting arginine arginine residues bladder catheterization codon Cohort cohort of patient component components contractile protein contractile proteins CpG de novo mutation de novo mutations dependent on described description diagnosed dinucleotide disease disorder DNA ENCODE enteric smooth muscle Families Finnish followed by food gastrointestinal problem gastrointestinal problems Genes heterozygous heterozygous mutation Hypoperistalsis identify initial intestinal intestinal pseudo-obstruction intestine less majority megacystis megacystis-microcolon-intestinal hypoperistalsis syndrome microcolon Missense mutation Missense mutations missense variant MMIHS muscle muscle contractile units muscle disease Muscle function Mutation mutations obstruction organ parenteral nutrition Patient patients phenotype powerful tool provide radiologist remained responsible Sanger sequencing Smooth smooth muscle smooth muscle disease Spectrum subjects syndrome the disease total parenteral nutrition Treatment unique urinary catheterization variant visceral whole-exome sequencing γ2 enteric actin [DOI] 10.1371/journal.pgen.1004258 PMC 바로가기 [Article Type] Research Article
Extreme Population Differences in the Human Zinc Transporter ZIP4 (SLC39A4) Are Explained by Positive Selection in Sub-Saharan Africa인간 아연 수송체 ZIP4(SLC39A4)의 극단적인 인구 차이는 사하라 사막 이남의 아프리카에서 양성 선택으로 설명됩니다Research Article Published on 2014-02-202022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] absence accompanied acrodermatitis acrodermatitis enteropathica African allele allele frequencies allele frequency Amino acid Author biological function Cell characterized classical computer simulations Concentration deficiency derived allele difference different directional disease phenotype disease risk DNA Evidence explained expression extreme finding fixation found functional Genetic Health HeLa cells homeostasis hotspot Human human population human populations implication intestinal investigated isoform leucine Local locus membrane Mutation natural selection Neanderthal Non-synonymous overexpression pathogen Pathogens polymorphism Population positive Positive selection Protein Recombination reduced reducing regulated Regulation Research Selection selective sweep significantly SLC39A4 sub-Saharan Africa surface protein Trace element transporter valine variant West Zinc zinc deficiency [DOI] 10.1371/journal.pgen.1004128 PMC 바로가기 [Article Type] Research Article
A Multigene Approach for Comparing Genealogy of Betacoronavirus from Cattle and HorsesResearch Article Published on 2013-11-172022-10-29 Journal: The Scientific World Journal [Category] Coronavirus, [키워드] analysed Bovine coronavirus Brazil cattle cause caused coronavirus coronavirus strain cow distribution equine Fecal sample found Gene genealogy Hemagglutinin-esterase horse infections intestinal morbidity and mortality N gene neonatal diarrhea Newborn partial sequence Protein Research sequence spike glycoprotein Transmission viruses [DOI] 10.1155/2013/349702 PMC 바로가기 [Article Type] Research Article
The evolution of codon usage in structural and non-structural viral genes: The case of Avian coronavirus and its natural host Gallus gallusArticle Published on 2013-09-302022-11-01 Journal: Virus research [Category] Coronavirus, [키워드] Avian coronavirus Gallus gallus 100% conserved Alpha Amino acid CAI cellular coded codon codon adaptation index codon usage compared coronavirus different driving effective Evolution GC Gene Genes Genetic glycoprotein Host host gene immune intestinal kidney less Lineage lung molecular molecular basis Mutation natural selection non-structural proteins nucleocapsid oviduct Papain-like protease predicted Protein protein A relative Replication respiratory tract significantly spike spike gene subunit synonymous Topology viral spike virus Virus–host Vitamin D receptor was measured were used [DOI] 10.1016/j.virusres.2013.09.033 PMC 바로가기 [Article Type] Article
Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronavirusesResearch Published on 2013-08-212022-10-28 Journal: Veterinary Research [Category] Coronavirus, MERS, [키워드] addition Cell culture circulating compared coronavirus Culture cultures death different epithelial evaluated Feline infectious peritonitis growth healthy Infection infectious inoculated intestinal intestinal epithelial cells lack mutant no infection observation replicated replication capacity Research serotype significantly more strain Strains susceptible susceptible cell Suspension SV40 telomerase tissue tissue homogenates virulent virus was done [DOI] 10.1186/1297-9716-44-71 PMC 바로가기 [Article Type] Research