Sample-to-answer, extraction-free, real-time RT-LAMP test for SARS-CoV-2 in nasopharyngeal, nasal, and saliva samples: Implications and use for surveillance testing비인두, 비강 및 타액 샘플에서 SARS-CoV-2에 대한 샘플 대 답변, 추출이 필요 없는 실시간 RT-LAMP 테스트: 감시 테스트에 대한 의미 및 사용Research Article Published on 2022-02-252022-09-12 Journal: PLoS ONE [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] asymptomatic individual Cohort cohort of patient collected COVID-19 COVID-19 pandemic effort extraction followed by Hospitalized include infected individuals Infectious disease isothermal isothermal amplification nasal nasal swabs nasopharyngeal nasopharyngeal swabs nucleic acid nucleic acid test per day performed positive protocol provide reagents Result Rice RT-LAMP RT-qPCR Saliva SARS-CoV-2 scaled Scaling Surveillance surveillance testing thermocycler Threats university viral inactivation Viral RNA [DOI] 10.1371/journal.pone.0264130 PMC 바로가기 [Article Type] Research Article
Development of rapid antigen test prototype for detection of SARS-CoV-2 in saliva samples타액 샘플에서 SARS-CoV-2 검출을 위한 신속 항원 검사 프로토타입 개발Original Article Published on 2022-02-252022-09-10 Journal: Upsala Journal of Medical Sciences [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 anti-RBD anti-receptor-binding domain Anti-spike antibody Antigen antigen test Asymptomatic capture Chain Reaction clinical analysis Cohort Combination combinations coronavirus coronavirus disease COVID-19 demonstrated detect detection antibody development diagnostic Diagnostic accuracy Diagnostic method diagnostic methods domain early stage ELISA ENhance enzyme enzyme-linked immunosorbent enzyme-linked immunosorbent assay evaluated exhibited followed by hospitalized COVID-19 patient hospitalized COVID-19 patients immunized improvement individual Infection interquartile range lateral flow lateral flow assay median time NPV onset of symptom pilot study point-of-care testing polyclonal antibodies polyclonal antibody polymerase chain reaction positive PPV RBD reference method respiratory Result reverse transcription Reverse transcription-polymerase chain reaction rRBD rRT-PCR Saliva saliva samples sample collection SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection SARS-CoV-2 spike protein SARS-CoV-2 virus sensitivity severe acute respiratory syndrome Coronavirus specificity Spike protein stratified symptomatic patient tested the median the SARS-CoV-2 validation study virus was used were used [DOI] 10.48101/ujms.v127.8207 PMC 바로가기 [Article Type] Original Article
Food for thought: Eating before saliva collection and interference with SARS‐CoV‐2 detectionResearch Article Published on 2022-02-232022-10-04 Journal: Journal of medical virology [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, [키워드] acute respiratory syndrome children collected coronavirus detection determine diagnostic diagnostics dietary habit eating enzyme fasting food healthy children interfere Interference intrinsic MALDI‐TOF molecular noninvasive nucleic acid oral microbiota performed polymerase chain provided replicate required resolved RT‐PCR Saliva saliva specimens SARS‐CoV‐2 SARS‐CoV‐2 selected specimen unique upper respiratory infection variety viral nucleic acid virus Volume was reduced [DOI] 10.1002/jmv.27660 PMC 바로가기 [Article Type] Research Article
Evaluation of the genetic risk for COVID-19 outcomes in COPD and differences among worldwide populationsResearch Article Published on 2022-02-232022-10-28 Journal: PLoS ONE [Category] COVID-19, COVID19(2023년), MERS, SARS, [키워드] acute respiratory syndrome African allele alleles allelic allelic combination Analysis Asian build Case-control characterized chronic obstructive clinical response clinically Cohort conducted contributing to control group COPD coronavirus COVID-19 COVID-19 infection COVID-19 susceptibility Diseases distribution European European population finding Frequency Genetic genetic background genetic heterogeneity genetic map Genetic predisposition genetic risk genotyped global pandemic groups heterogeneity Hospitalization Infection Italian outcome P-value people with COPD Phenotypes poor prognosis Population populations predisposition Probability Prognosis pulmonary disease recent Respiratory failure respond risk risk allele Risk scores rs657152 Saliva severe COVID-19 severity significant difference SNP Spanish survival susceptibility used to calculate Variability was performed were assessed [DOI] 10.1371/journal.pone.0264009 PMC 바로가기 [Article Type] Research Article
The University of Padua salivary-based SARS-CoV-2 surveillance program minimized viral transmission during the second and third pandemic waveResearch Article Published on 2022-02-232022-10-28 Journal: BMC Medicine [Category] COVID-19, COVID19(2023년), Fulltext, MERS, SARS, [키워드] activated Active surveillance age Asymptomatic Cohort conducted Contact tracing Contagion correlated cumulative distribution effective employee greater incidence individual Infection Laboratory limit mild symptoms molecular NPs older subject pandemic Participants performed positive Prevent Prospective Study Result Saliva salivary Salivary testing SARS-CoV-2 SARS-CoV-2 spread SARS-CoV-2 surveillance serum antibody significantly lower Spread subject subjects Surveillance Swab Symptoms Transport university University employees Veneto viral loads Viral transmission [DOI] 10.1186/s12916-022-02297-1 PMC 바로가기 [Article Type] Research Article
Evaluation of Swab-Seq as a scalable, sensitive assay for community surveillance of SARS-CoV-2 infectionArticle Published on 2022-02-232022-10-29 Journal: Scientific Reports [Category] COVID-19, MERS, [키워드] addition asymptomatic individual clinically collected Community developmental diagnostic Effectiveness effort evaluated expand gold standard independent individual Intensive lab less Modification nasal swab nasopharyngeal Next-generation sequencing noninvasive NP swab polymerase chain protocol qRT-PCR qRT-PCR assay Quantitative repeated resource reverse transcription RNA RNA sequencing Saliva SARS-COV-2 infection SARS-CoV-2 pandemic Scientific community stability subsequent Surveillance Swab symptomatic and asymptomatic utility viral infection [DOI] 10.1038/s41598-022-06901-5 PMC 바로가기 [Article Type] Article
Centrifugal Microfluidic Method for Enrichment and Enzymatic Extraction of Severe Acute Respiratory Syndrome Coronavirus 2 RNA중증 급성 호흡기 증후군 코로나바이러스 2 RNA의 농축 및 효소 추출을 위한 원심 미세 유체 방법Article Published on 2022-02-222022-09-11 Journal: Analytical Chemistry [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 amplification analyst analyzed applicability buffer capsid Centrifugal Chain Reaction comparable complex controls coronavirus coronavirus 2 demonstrated development Diagnosis diagnostics downstream effective ENhance enrichment exposure to extraction imperative in viral inhibit Intervention Isolation lysed nasopharyngeal Nasopharyngeal swab nucleic acid nucleic acids Particle pathogen detection pathogenic Pathogens platform polymerase chain polymerase chain reaction positive purification real-time reverse transcriptase real-time RT-PCR removal respiratory reverse transcriptase Reverse transcriptase polymerase chain reaction RNA RNA extraction robust RT-PCR Saliva Sample SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA SARS-CoV-2 virion severe acute respiratory syndrome Coronavirus Surveillance Transmission Transport media Treatment tube Viral RNA viral transport media virus [DOI] 10.1021/acs.analchem.1c05215 PMC 바로가기 [Article Type] Article
Evaluation of SARS-CoV-2 rapid antigen diagnostic tests for saliva samplesResearch Article Published on 2022-02-222022-10-31 Journal: Heliyon [Category] COVID-19, [키워드] Abbott Antigen cassette Cell culture comparable complement COVID-19 detect Detection limit diagnostic diagnostic test evaluated facilitate feasibility healthcare worker Hospitalized immunochromatographic test individuals with COVID-19 Infectious virus Infectivity limit of detection live SARS-CoV-2 Live virus Nasopharyngeal samples nine nucleocapsid protein positive Protein purified quantitative PCR Rapid Rapid antigen diagnostic test response reverse transcription RT-qPCR Saliva saliva test SARS-CoV-2 SARS-CoV-2 nucleocapsid SARS-CoV-2 positive individuals sensitivity Seven specificity Test tested virus was performed [DOI] 10.1016/j.heliyon.2022.e08998 PMC 바로가기 [Article Type] Research Article
Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of conceptArticle Published on 2022-02-182022-10-29 Journal: Scientific Reports [Category] COVID-19, COVID19(2023년), SARS, [키워드] Analysis coronavirus disease COVID-19 G protein human saliva information Lentiviral particle molecular test nasopharyngeal near-infrared spectroscopy optical Patient PCR-negative point-of-care pseudotyped Quantitative analysis reduce required saline solution Saliva SARS-CoV-2 SARS-CoV-2 viral loads screening strategy specificity symptomatic cases the SARS-CoV-2 the spike protein Transmission vesicular stomatitis virus viral infection [DOI] 10.1038/s41598-022-06393-3 PMC 바로가기 [Article Type] Article
Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screeningArticle Published on 2022-02-182022-10-04 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, [키워드] 95%CI analyzed collected demonstrated Diagnosis diagnostic Disease prevention dominant Epidemiology evaluated event Genetic variant Health authority individual Infectious-disease diagnostics isothermal amplification Mutation Outpatient Participants performance Population screening positive primer quantitative PCR reference standard required RT-LAMP RT-qPCR Saliva salivary SARS-CoV-2 SARS-CoV-2 detection SARS-COV-2 infection secure specificity Surveillance Swab symptomatic and asymptomatic tested transfer unique [DOI] 10.1038/s41598-022-04826-7 PMC 바로가기 [Article Type] Article