Longitudinal proteomic profiling provides insights into host response and proteome dynamics in COVID-19 progression세로 프로테옴 프로파일링은 COVID-19 진행에서 숙주 반응 및 프로테옴 역학에 대한 통찰력을 제공합니다Article Published on 2021-06-012022-09-11 Journal: Proteomics [Category] MERS, 바이오마커, 유전자 메커니즘, [키워드] acute phase response biological processe biological processes BNC2 collected coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 disease COVID-19 progression degranulation Disease progression expand high risk host response humoral immune response identify IGLV3-19 interferon involved Keywords lipid metabolism longitudinal longitudinal change longitudinal changes marker metabolism non-severe patients Patient patients with COVID-19 performed Platelet Potential biomarker potential biomarkers Prognosis prognostic prognostic indicator progressed progression Protein proteome proteomic proteomics provide Real-time monitoring SARS-COV-2 infection sera serum severe COVID-19 severity Support the disease [DOI] 10.1002/pmic.202000278 PMC 바로가기 [Article Type] Article
Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection단백질체 분석은 RNA 헬리카제 DDX3X를 SARS-CoV-2 감염에 대한 숙주 표적으로 식별합니다.Article Published on 2021-06-012022-09-11 Journal: Antiviral Research [Category] MERS, SARS, 유전자 메커니즘, 진단, 치료제, [키워드] accumulate aggregate antiviral drugs antiviral therapeutics cellular characterization COVID-19 cytoplasmic DDX3X DEAD RNA helicase develop eIF2α foci G3BP1 granules Health Helicase Host host protein host proteins Host-directed therapies identify infected cell infected cells infected Vero E6 cells Infection involved life cycle metabolism mitigate nucleoprotein Phosphorylation powerful tool Protein proteomic Proteomic analysis proteomics reduce Replication required ribonucleoprotein RNA SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 nucleoprotein SARS-CoV-2 replication scaffold proteins significantly small interfering RNAs small molecule Small molecules Stress stress granule stress granules Structure the SARS-CoV-2 Therapeutic strategies therapeutic strategy translation upstream Vero E6 VERO E6 cells Viral viral replication Viral RNA virus viruses [DOI] 10.1016/j.antiviral.2021.105064 PMC 바로가기 [Article Type] Article
Applications of Deep Mutational Scanning in VirologyReview Published on 2021-05-282022-10-28 Journal: Viruses [Category] COVID-19, [키워드] Activation Analysis application bioinformatics changed changes in ChIP-seq Complete coronavirus 2 CRISPR deep deep mutational scanning Dengue Epigenetic example experiment facilitate Flaviviridae functional genetics Genome Hepatitis human gene Infection Influenza molecular virology next-generation sequencing technology nucleotide probe Protein proteomics Region Research respiratory RNA virus SARS-CoV-2 scanning Viral protein viral replication Virology virus Zika [DOI] 10.3390/v13061020 PMC 바로가기 [Article Type] Review
Human cardiosphere-derived stromal cells exposed to SARS-CoV-2 evolve into hyper-inflammatory/pro-fibrotic phenotype and produce infective viral particles depending on the levels of ACE2 receptor expressionSARS-CoV-2에 노출된 인간 심장권 유래 기질 세포는 ACE2 수용체 발현 수준에 따라 과염증/친섬유증 표현형으로 진화하고 감염성 바이러스 입자를 생성합니다Article Published on 2021-05-252022-09-11 Journal: Cardiovascular Research [Category] SARS, 치료기술, [키워드] ACE2 ACE2 receptor analysed angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme-2 cardiac cardiac complication Cardiac complications cardiac injury Cardiac stromal cells caused Cell cellular Complication conditions COVID-19 demonstrated detect determine electron microscopy explain exposure to expression fibrosis High-resolution Human in vitro infected patients Infection infective Inflammation Injury Innate immunity involved Microscopy Myocardial Myocardium pathway PCR performed phenotype proteomics regulated reported RT-qPCR assay RT-qPCR assays SARS-CoV-2 SARS-COV-2 infection sensitivity Severe case severe cases severe respiratory syndrome stromal cell stromal cells supernatant susceptible these cell These cells transcriptomics undergo viral particle Viral particles virus [DOI] 10.1093/cvr/cvab082 PMC 바로가기 [Article Type] Article
Proteomics of extracellular vesicles in plasma reveals the characteristics and residual traces of COVID-19 patients without underlying diseases after 3 months of recovery혈장 내 세포외 소포체 단백질체학, 3개월 회복 후 기저질환 없는 코로나19 환자의 특성 및 잔류 흔적 밝혀Article Published on 2021-05-252022-09-11 Journal: Cell Death & Disease [Category] SARS, 바이오마커, 유전자 메커니즘, 진단, 치료기술, [키워드] addition Alanine alanine aminotransferase alkaline phosphatase ALP ALT association bleeding cellular change characteristic Characteristics Clinical characteristics Coagulation controls coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient Ct value defined differentially expressed discharge dysfunction EVs extracellular vesicle Extracellular vesicles functions healthy subject healthy subjects Identified immune response Immunity Increasing Inflammation inflammatory reaction Intervention involved kidney mass mass spectrometry metabolic process molecular organ organ dysfunction organ function oxygen Patient phosphatase plasma Prevent Protein proteome proteomics recovered COVID-19 patient Recovered COVID-19 patients recovered patient recovered patients Reinfection reported reveal serum electrolyte significantly lower situation Spectrometry Stress suffered underlying disease underlying diseases unique was used [DOI] 10.1038/s41419-021-03816-3 PMC 바로가기 [Article Type] Article
Heterogeneity of SARS-CoV-2 virus produced in cell culture revealed by shotgun proteomics and supported by genome sequencingResearch Paper Published on 2021-05-202022-10-28 Journal: Analytical and bioanalytical chemistry [Category] COVID-19, SARS, [키워드] Abstract amplification Analysis benefit Cell culture cell line change complementary consensus genome COVID-19 detectable diagnostic evade evaluated Evolution Genome sequencing heterogeneity immune response in-depth analysis induce marker mass spectrometry methodology molecular mutations pandemic Patient performed produced Production protein level proteomics purified Quality control SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 virus Sequencing Spread subject supplementary material supported Tandem mass spectrometry the SARS-CoV-2 genome the SARS-CoV-2 virus translated turn variant variants variations Vero E6 viral particle Viral particles while [DOI] 10.1007/s00216-021-03401-9 PMC 바로가기 [Article Type] Research Paper
Immune dysregulation and autoreactivity correlate with disease severity in SARS-CoV-2-associated multisystem inflammatory syndrome in children면역 조절 장애 및 자가 반응성은 소아의 SARS-CoV-2 관련 다기관 염증 증후군의 질병 중증도와 상관관계가 있습니다Article Published on 2021-05-112022-09-12 Journal: Immunity [Category] MERS, SARS, 진단, [키워드] alarmin alarmins Analysis Antigen Antigen presentation Asymptomatic Autoimmunity CD8 Cell characterized children correlated Course COVID-19 cytotoxicity defined diagnose disease severity dysfunction dysregulation elevated Evidence expression flow cytometry healthy IgG individual Infection Inflammation Inflammatory life-threatening memory T Mild MIS-C Patient pediatric plasmablasts predict proteomics receptor SARS-CoV-2 SARS-COV-2 infection serum Single-cell RNA sequencing skewed syndrome T cell TCR TRBV11-2 [DOI] 10.1016/j.immuni.2021.04.003 PMC 바로가기 [Article Type] Article
A rigorous evaluation of optimal peptide targets for MS-based clinical diagnostics of Coronavirus Disease 2019 (COVID-19)Research Published on 2021-05-102022-11-01 Journal: Clinical Proteomics [Category] COVID-19, [키워드] amplifying Analysis analyzed assays automated bioinformatics Care Cell culture clinical samples collected coronavirus coronavirus 2 COVID-19 COVID-19 infection status Cutoff dataset detect detection Diagnosis diagnosis of SARS-CoV-2 diagnostic diagnostics Evidence global pandemic highest human host in vitro Infection Limits of detection mass spectrometry membrane protein molecular nucleocapsid pandemic Patient peptide Peptide-detection Proteins proteomics quantitation respiratory Result SARS-CoV-2 seeking sequence specificity Spread sudden supplementary material target the SARS-CoV-2 virus upper respiratory tract viral peptide Viral proteome Viral RNA Visualization with COVID-19 Workflows [DOI] 10.1186/s12014-021-09321-1 PMC 바로가기 [Article Type] Research
Human Gut Microbiome and Liver Diseases: From Correlation to CausationReview Published on 2021-05-082022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] addition Affect benefit causes Chronic Liver Diseases cirrhosis contribute COVID-19 derivative DNA sequencing Evidence fibrosis Germ-free animal Gut gut microbiota help Hepatocellular carcinoma host immunity Human immune system Inflammation Interaction liver Liver disease liver diseases Liver injury mechanism metabolite metabolites metabolomics microbe microbial Microbiome microbiota molecular multiple organs Pathogenesis phenotype proteomics Specific stages steatohepatitis systemic disorder Therapies translocation virus [DOI] 10.3390/microorganisms9051017 PMC 바로가기 [Article Type] Review
Short chain fatty acids delay the development of hepatocellular carcinoma in HBx transgenic miceOriginal Research Published on 2021-05-012023-06-03 Journal: Neoplasia (New York, N.Y.) [Category] B형 간염, [키워드] chronic liver disease Hepatitis B x Pathogenesis proteomics Ras signaling [DOI] 10.1016/j.neo.2021.04.004 PMC 바로가기 [Article Type] Original Research