flow cytometry – Page 35 – Communicable Diseases Research

Circulating Cytokines and Lymphocyte Subsets in Patients Who Have Recovered from COVID-19

Research Article

Published on 2020-11-262022-10-27
Journal: BioMed Research International [Category] Coronavirus, COVID-19, MERS, SARS,

Immunological imprint of COVID‐19 on human peripheral blood leukocyte populations

인간 말초 혈액 백혈구 개체군에 대한 COVID-19의 면역학적 각인

Original Article

Published on 2020-11-222022-09-01
Journal: Allergy [Category] SARS, 진단, 치료법,

[키워드] activated Activation acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adaptive addition antibody antibody levels B cells CD25 CD3 CD31 CD38 CD4 CD45 CD45RA CD8 Cell cells cellular Cellular immune response cellular immune responses central memory clinical immunology Cohort convalescent patient Convalescent patients coronavirus coronavirus disease Coronavirus disease 2019 correlated COVID‐19 COVID‐19 patient COVID‐19 patients determined by disease domain effector memory elevated ELISA expression flow cytometry FOXP3 healthy control HLA‐DR immune responses immune system immunological immunological change immunological changes immunosorbent Infection infections innate immune system investigated leukocyte population Lymphocytes Mild Mild disease multiparametric flow cytometry neutrophil Neutrophils nucleocapsid pandemic parameters Patient patients Peripheral blood qPCR Quantitative quantitative PCR RBD receptor receptor‐binding domain Recombination reduction reflected regression analysis respiratory responses Result SARS‐CoV‐2 SARS‐CoV‐2 infection SARS‑CoV2 SARS‐CoV‐2 sense sense of taste severe acute respiratory syndrome Coronavirus significantly Spike protein subject T cell T cells T regulatory cell T regulatory cells T‐cell Taste thymic emigrant thymic emigrants transitional B cell transitional B cells triggered Viral
[DOI] 10.1111/all.14647 PMC 바로가기 [Article Type] Original Article

Early changes in immune cell subsets with corticosteroids in patients with solid tumors: implications for COVID-19 management

Immunotherapy Biomarkers

Published on 2020-11-202022-10-30
Journal: Journal for immunotherapy of cancer [Category] COVID-19, SARS,

[키워드] Absolute lymphocyte count absolute neutrophil count activated administration affected ALC ANC antiviral immunity antiviral responses Cancer CD4 cell CD8 cells cellular changes in Combination complex coronavirus Corticosteroid Corticosteroids COVID-19 Critical Cytometry Dexamethasone dose driving effective effector memory elevated enrolled Flow flow cytometry glucocorticoid High-dose hyperinflammation immune Immune cell Immunity immunomodulation Immunotherapy implication include increase in increased mortality Inflammation mediators Inflammatory lymphocyte lymphopenia management marker median Methylprednisolone Neutrophil-to-lymphocyte ratio NLR Patient Patients with cancer performed poor prognosis Prednisone reducing relative Result Retrospective analysis SARS-CoV-2 pandemic significant decrease significantly solid tumor statistically significant subset systemic inflammation T-lymphocytes therapy for COVID-19 Trial variety Wilcoxon signed-rank test
[DOI] 10.1136/jitc-2020-001019 PMC 바로가기 [Article Type] Immunotherapy Biomarkers

Thymosin Alpha 1 Reduces the Mortality of Severe Coronavirus Disease 2019 by Restoration of Lymphocytopenia and Reversion of Exhausted T Cells

티모신 알파 1은 림프구 감소증의 회복과 소진된 T 세포의 회복을 통해 심각한 코로나바이러스 질병 2019의 사망률을 감소시킵니다

Article

Published on 2020-11-192022-09-11
Journal: Clinical Infectious Diseases: An Official Publicat [Category] SARS, 유전자 메커니즘, 치료기술,

[키워드] (PD-1 Alpha benefit Blood CD4+ T cell CD4+ T cells CD4+ T-cell CD8+ CD8+ T cell CD8+ T cells circulation clinical benefit clinical benefits Clinical outcome clinical outcomes conditions COVID-19 COVID-19 patient COVID-19 patients domain elderly patients expression flow cytometry hospital immune immune reconstitution immune response Immunoglobulin Infection less lymphocytopenia marker mechanism mononuclear cell mononuclear cells Mortality mucin Patient PD-1 Peripheral blood Peripheral blood mononuclear cells protein 3 reduced respiratory restoration restored reversed reversion SARS-CoV-2 Severe acute respiratory syndrome severe COVID-19 severe COVID-19 patient severe COVID-19 patients significantly T cell T cells T-cell T-cell exhaustion T-cell receptor thymosin thymosin alpha 1 Treatment treatment for COVID-19 Viral viral infection viral infections was measured Wuhan Wuhan, China
[DOI] 10.1093/cid/ciaa630 PMC 바로가기 [Article Type] Article

Major reduction of NKT cells in patients with severe COVID-19 pneumonia

Article

Published on 2020-11-132023-07-02
Journal: Clinical immunology (Orlando, Fla.) [Category] SARS,

[키워드] CD56bright CD56dim flow cytometry Immunophenotyping analysis SARS-CoV-2
[DOI] 10.1016/j.clim.2020.108630 PMC 바로가기

Dynamics of NK, CD8 and Tfh cell mediated the production of cytokines and antiviral antibodies in Chinese patients with moderate COVID‐19

NK, CD8 및 Tfh 세포의 역학은 중등도 COVID-19 환자에서 사이토카인 및 항바이러스 항체 생성을 매개합니다

Original Article

Published on 2020-11-032022-09-12
Journal: Journal of Cellular and Molecular Medicine [Category] MERS, SARS, 진단,

[키워드] abnormal increase acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 analysed antibody antiviral antibody B‐cell benefit caused CD226 CD4 CD8 Cell cell count cellular change Chinese patient contribute control patient control patients convalescent convalescent patient coronavirus coronavirus disease Coronavirus disease 2019 COVID‐19 COVID‐19 cytokine cytokine level cytokine levels Cytokines decrease demonstrated Dynamics effector effector memory electrochemiluminescence immunoassay enrolled expression flow cytometry iCoS immune responses increase in Inflammatory cytokine Inflammatory cytokines inhibitory innate immune response lymphocyte maintenance memory moderate NK NK cell NK cells Patient patients with moderate peripheral lymphocyte Pneumonia Prevent recent respiratory RT‐PCR SARS‐CoV‐2 SARS‐CoV‐2‐specific antibodies SARS‐CoV‐2‐specific antibody severe acute respiratory syndrome Coronavirus Severe case severe cases significantly higher significantly increased subset T cell T cells T‐cell Tfh TIM‐3
[DOI] 10.1111/jcmm.16044 PMC 바로가기 [Article Type] Original Article

A Novel Human Skin Tissue Model To Study Varicella-Zoster Virus and Human Cytomegalovirus

수두-대상포진 바이러스와 인간 거대세포바이러스를 연구하기 위한 새로운 인간 피부 조직 모델

Article

Published on 2020-10-272023-05-31
Journal: Journal of Virology [Category] Fulltext, 대상포진, 두창, 수두, 홍역,

[키워드] crawl-out assay flow cytometry histology Human Cytomegalovirus SCID-Hu mouse skin organ culture Varicella-Zoster Virus
[DOI] 10.1128/JVI.01082-20 PMC 바로가기

Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine

Research

Published on 2020-10-232022-10-28
Journal: Virology Journal [Category] Coronavirus,

[키워드] β-propiolactone assessment Binary-ethylenimine cause caused CD4 CD8 cellular cellular immunity characterized control group demonstrated diarrhea Effect effective ELISA evaluated experimental group flow cytometry formaldehyde gastroenteritis group highest histopathological Histopathology Humoral immunity IFN-γ IgG IL-4 immunization inactivated Inactivated vaccine indicated induce Infection lymphocyte macroscopic mice Mortality pathological change pathology performed Peripheral blood positive rate produced proliferation reagent Result spleen subset T lymphocyte TGEV the disease transmissible gastroenteritis virus Transmissible gastroenteritis virus (TGEV) Vaccine virus vomiting
[DOI] 10.1186/s12985-020-01433-8 PMC 바로가기 [Article Type] Research

Rapid response flow cytometric assay for the detection of antibody responses to SARS-CoV-2

SARS-CoV-2에 대한 항체 반응 검출을 위한 신속한 반응 유세포 분석법

Original Article

Published on 2020-10-202022-09-10
Journal: European Journal of Clinical Microbiology & Infect [Category] MERS, SARS, 변종, 진단, 치료기술,

[키워드] 293T cell ACE-2 adopted angiotensin antibodies Antibody Response Antibody responses approach blueprint CLIA coronavirus detect diagnostic early phase ELISA evaluated expressing flow cytometric flow cytometry highest IgG and IgM IgM antibody Laboratory nucleic acid pandemic patient sera PCR-confirmed provide quantification Quantitative Rapid robust SARS-CoV-2 SARS-CoV-2 spike protein SARS-CoV-2-infected patients SARS-CoV-2-specific antibodies sensitivity sera Serological assay serological test Serological tests serology specific antibodies specificity specimen Spike protein Spike-specific antibody spike-specific antibody response Spike-specific antibody responses Spread variant Viral viral infection viral infections were used while Zoonotic coronavirus
[DOI] 10.1007/s10096-020-04072-7 PMC 바로가기 [Article Type] Original Article

Silent hypoxia: higher NO in red blood cells of COVID-19 patients

Research Article

Published on 2020-10-162022-10-28
Journal: BMC Pulmonary Medicine [Category] COVID-19, Fulltext, MERS, SARS,

[키워드] acute respiratory distress acute respiratory syndrome ARDS blood sample cause control group coronavirus coronavirus disease COVID-19 COVID-19 patient COVID-19 patients Cytokine storm Cytokines death Diffuse alveolar damage disease evaluated FACS flow cytometry Future Gas exchange healthy control hypoxia hypoxic hypoxic patients infected case intensity interstitial thickening Intracellular Iran mechanism nitric oxide NO Non-COVID-19 non-COVID-19 patient Pathogenesis Patient phenotype pilot study pulmonary involvement RBC recruited red blood cell Respiratory failure Result SARS-CoV-2 Serum level severe COVID-19 severe COVID-19 patients significant difference significantly spread to staining subject subjects syndrome Tehran therapy USA Ventilation vessels was measured
[DOI] 10.1186/s12890-020-01310-8 PMC 바로가기 [Article Type] Research Article